Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fmgV1a16r
(926 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 341 9e-95
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 341 9e-95
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 341 9e-95
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 103 3e-23
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 64 4e-11
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 55 1e-08
SPBP35G2.13c |swc2||chromatin remodeling complex subunit Swc2 |S... 27 2.8
SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subuni... 27 5.0
SPBC21D10.06c |map4||cell agglutination protein Map4|Schizosacch... 26 6.6
SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal protein... 26 8.7
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 341 bits (838), Expect = 9e-95
Identities = 158/214 (73%), Positives = 181/214 (84%)
Frame = -3
Query: 903 LKLLDAILPPARPTDKPPASFPCKTYTKXGGIGTVPVGRVETGVLKPGTIVVFAPANITT 724
L+ +D+I PPARPTDKP P + K GGIGTVPVGRVETGV+KPG IV FAPA +TT
Sbjct: 227 LEAIDSIEPPARPTDKP-LRLPLQDVYKIGGIGTVPVGRVETGVIKPGMIVTFAPAGVTT 285
Query: 723 EVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVL 544
EVKSVEMHHE+L +PGDNVGFNVKNVSVK++RRG V GDSKN+PP G A FTAQVI+L
Sbjct: 286 EVKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIIL 345
Query: 543 NHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPS 364
NHPGQIS GY+PVLDCHTAHIACKFAE+ EK+DRR+GK E +PK +KSGDA I +VPS
Sbjct: 346 NHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPS 405
Query: 363 KPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAV 262
KP+CVE+F ++ PLGRFAVRDMRQTVAVGVIKAV
Sbjct: 406 KPMCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAV 439
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 341 bits (838), Expect = 9e-95
Identities = 158/214 (73%), Positives = 181/214 (84%)
Frame = -3
Query: 903 LKLLDAILPPARPTDKPPASFPCKTYTKXGGIGTVPVGRVETGVLKPGTIVVFAPANITT 724
L+ +D+I PPARPTDKP P + K GGIGTVPVGRVETGV+KPG IV FAPA +TT
Sbjct: 227 LEAIDSIEPPARPTDKP-LRLPLQDVYKIGGIGTVPVGRVETGVIKPGMIVTFAPAGVTT 285
Query: 723 EVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVL 544
EVKSVEMHHE+L +PGDNVGFNVKNVSVK++RRG V GDSKN+PP G A FTAQVI+L
Sbjct: 286 EVKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIIL 345
Query: 543 NHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPS 364
NHPGQIS GY+PVLDCHTAHIACKFAE+ EK+DRR+GK E +PK +KSGDA I +VPS
Sbjct: 346 NHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPS 405
Query: 363 KPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAV 262
KP+CVE+F ++ PLGRFAVRDMRQTVAVGVIKAV
Sbjct: 406 KPMCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAV 439
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 341 bits (838), Expect = 9e-95
Identities = 158/214 (73%), Positives = 181/214 (84%)
Frame = -3
Query: 903 LKLLDAILPPARPTDKPPASFPCKTYTKXGGIGTVPVGRVETGVLKPGTIVVFAPANITT 724
L+ +D+I PPARPTDKP P + K GGIGTVPVGRVETGV+KPG IV FAPA +TT
Sbjct: 227 LEAIDSIEPPARPTDKP-LRLPLQDVYKIGGIGTVPVGRVETGVIKPGMIVTFAPAGVTT 285
Query: 723 EVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVL 544
EVKSVEMHHE+L +PGDNVGFNVKNVSVK++RRG V GDSKN+PP G A FTAQVI+L
Sbjct: 286 EVKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIIL 345
Query: 543 NHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPS 364
NHPGQIS GY+PVLDCHTAHIACKFAE+ EK+DRR+GK E +PK +KSGDA I +VPS
Sbjct: 346 NHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPS 405
Query: 363 KPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAV 262
KP+CVE+F ++ PLGRFAVRDMRQTVAVGVIKAV
Sbjct: 406 KPMCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAV 439
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 103 bits (248), Expect = 3e-23
Identities = 66/217 (30%), Positives = 112/217 (51%), Gaps = 2/217 (0%)
Frame = -3
Query: 903 LKLLDAILPPARPTDKPPASFPCKTYTKXGGIGTVPVGRVETGVLKPGTIVVFAPANITT 724
L+ LD++ R + P F +K +GT+ G++E G +K + V+ P N T
Sbjct: 452 LEYLDSMTHLERKVNAP---FIMPIASKYKDLGTILEGKIEAGSIKKNSNVLVMPINQTL 508
Query: 723 EVKSV-EMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIV 547
EV ++ + E + ++ GD V V+ +++ GYV +KN P F AQ+ +
Sbjct: 509 EVTAIYDEADEEISSSICGDQVRLRVRGDD-SDVQTGYVLTSTKN-PVHATTRFIAQIAI 566
Query: 546 LNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVP 367
L P ++ GY+ V+ HTA FA++ K+D+ T + ++ P G I L
Sbjct: 567 LELPSILTTGYSCVMHIHTAVEEVSFAKLLHKLDK-TNRKSKKPPMFATKGMKIIAELET 625
Query: 366 SKPLCVESFQEFPPLGRFAVRDMRQTVAVG-VIKAVN 259
P+C+E F+++ +GRF +RD TVAVG V+K ++
Sbjct: 626 QTPVCMERFEDYQYMGRFTLRDQGTTVAVGKVVKILD 662
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 63.7 bits (148), Expect = 4e-11
Identities = 60/213 (28%), Positives = 94/213 (44%), Gaps = 1/213 (0%)
Frame = -3
Query: 903 LKLLDAILPPARPTDKPPASFPCKTYTKXGGIGTVPVGRVETGVLKPGTIVVFAPANITT 724
L LD ++PP +P KP Y + TV GRVE G ++ ++ +
Sbjct: 388 LSALDQLVPPEKPYRKPLRLSIDDVYRSPRSV-TV-TGRVEAGNVQVNQVLYDVSSQEDA 445
Query: 723 EVKSVEMHHEALQE-AVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIV 547
VK+V + + AV GD V + ++ V +LR G + + +N P + F A++
Sbjct: 446 YVKNVIRNSDPSSTWAVAGDTVTLQLADIEVNQLRPGDILSNYEN-PVRRVRSFVAEIQT 504
Query: 546 LNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVP 367
+ G I +G T VL H+ + K+ K + + S K I L
Sbjct: 505 FDIHGPILSGSTLVL-----HLGRTVTSVSLKIVTVNNKRSR-HIASRKRALVRISFLDG 558
Query: 366 SKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIK 268
PLC+ +E P LGRF +R TVA G++K
Sbjct: 559 LFPLCLA--EECPALGRFILRRSGDTVAAGIVK 589
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 55.2 bits (127), Expect = 1e-08
Identities = 56/220 (25%), Positives = 93/220 (42%), Gaps = 6/220 (2%)
Frame = -3
Query: 912 NASLKLLDA----ILPPARPTDKPPASFPCKTYTKXGGIGTVPVGRVETGVLKPGTIV-- 751
N+ KL++A I P R TD P + G GTV GRVE G LK G +
Sbjct: 229 NSITKLMEAVDSYITLPERKTDVP-FLMAIEDVFSISGRGTVVTGRVERGTLKKGAEIEI 287
Query: 750 VFAPANITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAA 571
V +++ T V +EM + L AV GDN G ++++ ++L+RG + P
Sbjct: 288 VGYGSHLKTTVTGIEMFKKQLDAAVAGDNCGLLLRSIKREQLKRGMIVAQPGTVAPH--Q 345
Query: 570 DFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGD 391
F A +L + T +D + + + +++ ++ T + K + GD
Sbjct: 346 KFKASFYILTK--EEGGRRTGFVDKYRPQLYSRTSDVTVEL---THPDPNDSDKMVMPGD 400
Query: 390 AAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVI 271
+ P+ +E Q RF VR+ TV ++
Sbjct: 401 NVEMICTLIHPIVIEKGQ------RFTVREGGSTVGTALV 434
>SPBP35G2.13c |swc2||chromatin remodeling complex subunit Swc2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 316
Score = 27.5 bits (58), Expect = 2.8
Identities = 12/35 (34%), Positives = 17/35 (48%)
Frame = -3
Query: 924 KLTENASLKLLDAILPPARPTDKPPASFPCKTYTK 820
K+ +N + L + PP RPT + P K Y K
Sbjct: 95 KIQKNRAANLQRTLQPPKRPTPSAASEVPKKKYKK 129
>SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subunit a
Pol2 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 2199
Score = 26.6 bits (56), Expect = 5.0
Identities = 12/25 (48%), Positives = 15/25 (60%)
Frame = +1
Query: 625 QFLDGHVLYVETYIVSRYSFLESFV 699
+F DGH+L ETY+ LES V
Sbjct: 527 KFFDGHLLASETYVGGHVESLESGV 551
>SPBC21D10.06c |map4||cell agglutination protein
Map4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 948
Score = 26.2 bits (55), Expect = 6.6
Identities = 13/32 (40%), Positives = 20/32 (62%)
Frame = +2
Query: 719 TSVVMLAGAKTTMVPGFNTPVSTLPTGTVPIP 814
+SVV+ + +T V + + VST TGTV +P
Sbjct: 86 SSVVLYSAKETVTVSSYWSLVSTSVTGTVYVP 117
>SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 670
Score = 25.8 bits (54), Expect = 8.7
Identities = 24/85 (28%), Positives = 42/85 (49%), Gaps = 2/85 (2%)
Frame = +2
Query: 218 SAALVTLPPPASLKLTALMTPTATVCLMSRTAKRPRGGNSWKDSTH--RGLEGTKLTMAA 391
S +VTLPPPAS ++ T T T + S ++ G+ + +++ + ++++
Sbjct: 183 STDIVTLPPPAS-STSSFSTITNTSMIPSSSSFTTTTGSPYYNTSSFLPSSVISSASLSS 241
Query: 392 SPDLMDFGLTSVDLPVRRSTFSLIS 466
S L +TS PV S+ SL S
Sbjct: 242 SSVLPTSIITSTSTPVTVSSSSLSS 266
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,865,059
Number of Sequences: 5004
Number of extensions: 82134
Number of successful extensions: 257
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 238
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 248
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 469338710
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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