Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fmgV19j02r
(891 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 364 e-101
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 364 e-101
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 364 e-101
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 109 5e-25
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 79 6e-16
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 70 4e-13
SPBC21D10.06c |map4||cell agglutination protein Map4|Schizosacch... 27 3.6
SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subuni... 27 4.7
SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 27 4.7
SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal protein... 26 8.3
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 364 bits (895), Expect = e-101
Identities = 166/210 (79%), Positives = 187/210 (89%)
Frame = -1
Query: 891 LDAILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPANITTEVKS 712
+D+I PPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGV+KPG IV FAPA +TTEVKS
Sbjct: 230 IDSIEPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVIKPGMIVTFAPAGVTTEVKS 289
Query: 711 VEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPG 532
VEMHHE+L +PGDNVGFNVKNVSVK++RRG V GDSKN+PP G A FTAQVI+LNHPG
Sbjct: 290 VEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNHPG 349
Query: 531 QISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLC 352
QIS GY+PVLDCHTAHIACKFAE+ EK+DRR+GK E +PK +KSGDA I +VPSKP+C
Sbjct: 350 QISAGYSPVLDCHTAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMC 409
Query: 351 VESFQEFPPLGRFAVRDMRQTVAVGVIKAV 262
VE+F ++ PLGRFAVRDMRQTVAVGVIKAV
Sbjct: 410 VEAFTDYAPLGRFAVRDMRQTVAVGVIKAV 439
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 364 bits (895), Expect = e-101
Identities = 166/210 (79%), Positives = 187/210 (89%)
Frame = -1
Query: 891 LDAILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPANITTEVKS 712
+D+I PPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGV+KPG IV FAPA +TTEVKS
Sbjct: 230 IDSIEPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVIKPGMIVTFAPAGVTTEVKS 289
Query: 711 VEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPG 532
VEMHHE+L +PGDNVGFNVKNVSVK++RRG V GDSKN+PP G A FTAQVI+LNHPG
Sbjct: 290 VEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNHPG 349
Query: 531 QISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLC 352
QIS GY+PVLDCHTAHIACKFAE+ EK+DRR+GK E +PK +KSGDA I +VPSKP+C
Sbjct: 350 QISAGYSPVLDCHTAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMC 409
Query: 351 VESFQEFPPLGRFAVRDMRQTVAVGVIKAV 262
VE+F ++ PLGRFAVRDMRQTVAVGVIKAV
Sbjct: 410 VEAFTDYAPLGRFAVRDMRQTVAVGVIKAV 439
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 364 bits (895), Expect = e-101
Identities = 166/210 (79%), Positives = 187/210 (89%)
Frame = -1
Query: 891 LDAILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPANITTEVKS 712
+D+I PPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGV+KPG IV FAPA +TTEVKS
Sbjct: 230 IDSIEPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVIKPGMIVTFAPAGVTTEVKS 289
Query: 711 VEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPG 532
VEMHHE+L +PGDNVGFNVKNVSVK++RRG V GDSKN+PP G A FTAQVI+LNHPG
Sbjct: 290 VEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNHPG 349
Query: 531 QISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLC 352
QIS GY+PVLDCHTAHIACKFAE+ EK+DRR+GK E +PK +KSGDA I +VPSKP+C
Sbjct: 350 QISAGYSPVLDCHTAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMC 409
Query: 351 VESFQEFPPLGRFAVRDMRQTVAVGVIKAV 262
VE+F ++ PLGRFAVRDMRQTVAVGVIKAV
Sbjct: 410 VEAFTDYAPLGRFAVRDMRQTVAVGVIKAV 439
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 109 bits (262), Expect = 5e-25
Identities = 66/213 (30%), Positives = 112/213 (52%), Gaps = 2/213 (0%)
Frame = -1
Query: 891 LDAILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPANITTEVKS 712
LD++ R + P +P+ YK +GT+ G++E G +K + V+ P N T EV +
Sbjct: 455 LDSMTHLERKVNAPFIMPIASKYK--DLGTILEGKIEAGSIKKNSNVLVMPINQTLEVTA 512
Query: 711 V-EMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHP 535
+ + E + ++ GD V V+ +++ GYV +KN P F AQ+ +L P
Sbjct: 513 IYDEADEEISSSICGDQVRLRVRGDD-SDVQTGYVLTSTKN-PVHATTRFIAQIAILELP 570
Query: 534 GQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPL 355
++ GY+ V+ HTA FA++ K+D+ T + ++ P G I L P+
Sbjct: 571 SILTTGYSCVMHIHTAVEEVSFAKLLHKLDK-TNRKSKKPPMFATKGMKIIAELETQTPV 629
Query: 354 CVESFQEFPPLGRFAVRDMRQTVAVG-VIKAVN 259
C+E F+++ +GRF +RD TVAVG V+K ++
Sbjct: 630 CMERFEDYQYMGRFTLRDQGTTVAVGKVVKILD 662
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 79.4 bits (187), Expect = 6e-16
Identities = 64/209 (30%), Positives = 99/209 (47%), Gaps = 1/209 (0%)
Frame = -1
Query: 891 LDAILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPANITTEVKS 712
LD ++PP +P KPLRL + DVY+ TV GRVE G ++ ++ + VK+
Sbjct: 391 LDQLVPPEKPYRKPLRLSIDDVYRSPRSVTV-TGRVEAGNVQVNQVLYDVSSQEDAYVKN 449
Query: 711 VEMHHEALQE-AVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHP 535
V + + AV GD V + ++ V +LR G + + + NP + F A++ +
Sbjct: 450 VIRNSDPSSTWAVAGDTVTLQLADIEVNQLRPGDILSNYE-NPVRRVRSFVAEIQTFDIH 508
Query: 534 GQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPL 355
G I +G T VL H+ + K+ K + + S K I L PL
Sbjct: 509 GPILSGSTLVL-----HLGRTVTSVSLKIVTVNNKRSR-HIASRKRALVRISFLDGLFPL 562
Query: 354 CVESFQEFPPLGRFAVRDMRQTVAVGVIK 268
C+ +E P LGRF +R TVA G++K
Sbjct: 563 CLA--EECPALGRFILRRSGDTVAAGIVK 589
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 70.1 bits (164), Expect = 4e-13
Identities = 54/203 (26%), Positives = 91/203 (44%), Gaps = 2/203 (0%)
Frame = -1
Query: 873 PARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGT--IVVFAPANITTEVKSVEMH 700
P R TD P + ++DV+ I G GTV GRVE G LK G +V +++ T V +EM
Sbjct: 245 PERKTDVPFLMAIEDVFSISGRGTVVTGRVERGTLKKGAEIEIVGYGSHLKTTVTGIEMF 304
Query: 699 HEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISN 520
+ L AV GDN G ++++ ++L+RG + P F A +L +
Sbjct: 305 KKQLDAAVAGDNCGLLLRSIKREQLKRGMIVAQPGTVAPH--QKFKASFYILTK--EEGG 360
Query: 519 GYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESF 340
T +D + + + +++ ++ T + K + GD + P+ +E
Sbjct: 361 RRTGFVDKYRPQLYSRTSDVTVEL---THPDPNDSDKMVMPGDNVEMICTLIHPIVIEKG 417
Query: 339 QEFPPLGRFAVRDMRQTVAVGVI 271
Q RF VR+ TV ++
Sbjct: 418 Q------RFTVREGGSTVGTALV 434
>SPBC21D10.06c |map4||cell agglutination protein
Map4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 948
Score = 27.1 bits (57), Expect = 3.6
Identities = 14/39 (35%), Positives = 22/39 (56%)
Frame = +2
Query: 719 TSVVMLAGAKTTMVPGFNTPVSTLPTGTVPIPPILYTSC 835
+SVV+ + +T V + + VST TGTV +P +C
Sbjct: 86 SSVVLYSAKETVTVSSYWSLVSTSVTGTVYVPYTSSVAC 124
>SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subunit a
Pol2 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 2199
Score = 26.6 bits (56), Expect = 4.7
Identities = 12/25 (48%), Positives = 15/25 (60%)
Frame = +1
Query: 625 QFLDGHVLYVETYIVSRYSFLESFV 699
+F DGH+L ETY+ LES V
Sbjct: 527 KFFDGHLLASETYVGGHVESLESGV 551
>SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 646
Score = 26.6 bits (56), Expect = 4.7
Identities = 23/89 (25%), Positives = 39/89 (43%), Gaps = 5/89 (5%)
Frame = -1
Query: 891 LDAILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPANITTEVKS 712
+ I PP + PLR L D + G + + R+ G +K G V+ EV+
Sbjct: 230 IQKIPPPKGSENAPLRCLLIDSWYNSYQGVIGLVRIMEGFIKKGGKVMSVNTGRKYEVQQ 289
Query: 711 VEMHHEALQEA--VPGDNVGF---NVKNV 640
V + + + E + VG+ N+KN+
Sbjct: 290 VGIMYPDMTEVSRLRAGQVGYIIWNMKNI 318
>SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 670
Score = 25.8 bits (54), Expect = 8.3
Identities = 24/85 (28%), Positives = 42/85 (49%), Gaps = 2/85 (2%)
Frame = +2
Query: 218 SAALVTLPPPASLKLTALMTPTATVCLMSRTAKRPRGGNSWKDSTH--RGLEGTKLTMAA 391
S +VTLPPPAS ++ T T T + S ++ G+ + +++ + ++++
Sbjct: 183 STDIVTLPPPAS-STSSFSTITNTSMIPSSSSFTTTTGSPYYNTSSFLPSSVISSASLSS 241
Query: 392 SPDLMDFGLTSVDLPVRRSTFSLIS 466
S L +TS PV S+ SL S
Sbjct: 242 SSVLPTSIITSTSTPVTVSSSSLSS 266
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,740,092
Number of Sequences: 5004
Number of extensions: 79156
Number of successful extensions: 255
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 237
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 251
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 448490560
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -