BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fmgV19e03r
(842 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC13C5.02 |dre4||DNA replication protein Dre4|Schizosaccharomy... 30 0.36
SPAC4G9.07 |mug133||S. pombe specific UPF0300 family protein 2|S... 27 2.5
SPAC1782.03 |||microfibrillar-associated protein family protein|... 27 4.4
SPAC821.05 |||translation initiation factor eIF3h|Schizosaccharo... 27 4.4
SPAC27F1.04c |nuf2||spindle pole body protein Nuf2|Schizosacchar... 26 5.8
SPBC1709.06 |dus2||tRNA dihydrouridine synthase Dus2 |Schizosacc... 26 7.7
SPAC4F8.13c |rng2||IQGAP|Schizosaccharomyces pombe|chr 1|||Manual 26 7.7
>SPAC13C5.02 |dre4||DNA replication protein Dre4|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 411
Score = 30.3 bits (65), Expect = 0.36
Identities = 20/71 (28%), Positives = 31/71 (43%)
Frame = -1
Query: 533 LGTPIKKVVTSEIPCIDKLQKSLVMGSTKNKGEILYPGDVKIKVAEPKGLNALHLEDVIY 354
L PI+K ++ D Q V N EI ++ ++ EP GL E V+Y
Sbjct: 130 LRLPIRKQISK-----DSSQSQNVDSGKTNHEEIHESRHLQTEIEEPSGLEESSEESVLY 184
Query: 353 KTLVYDKKEDE 321
Y+K ++E
Sbjct: 185 SEEFYEKSDEE 195
>SPAC4G9.07 |mug133||S. pombe specific UPF0300 family protein
2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 513
Score = 27.5 bits (58), Expect = 2.5
Identities = 10/26 (38%), Positives = 18/26 (69%)
Frame = +3
Query: 189 LLCNYFEQSLESALYVAINSYIYSYC 266
+L +Y+ L+SAL+ A++SY+ C
Sbjct: 253 MLYHYYPSFLQSALWTAVSSYLEERC 278
>SPAC1782.03 |||microfibrillar-associated protein family
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 355
Score = 26.6 bits (56), Expect = 4.4
Identities = 16/54 (29%), Positives = 26/54 (48%)
Frame = -1
Query: 590 LTPSEFQQLVRDVRVIEASLGTPIKKVVTSEIPCIDKLQKSLVMGSTKNKGEIL 429
L P +F V R T + K VTS+ + +++ L++ TKN E+L
Sbjct: 126 LLPPKFTSKVIKNRAKANEEDTEVLKKVTSQKILEETIKRELLLKETKNNNELL 179
>SPAC821.05 |||translation initiation factor
eIF3h|Schizosaccharomyces pombe|chr 1|||Manual
Length = 357
Score = 26.6 bits (56), Expect = 4.4
Identities = 18/53 (33%), Positives = 26/53 (49%), Gaps = 11/53 (20%)
Frame = -1
Query: 836 IISSDHKQFCLLHCISAYPVP---------FEDC--NLTVLQDYKNTFDVPVG 711
+I + H CLLH +S P P EDC NL + + + N F+V +G
Sbjct: 193 VIHNSHLATCLLHSLSEPPTPASTLTAEAALEDCESNLPLTETFSN-FEVSLG 244
>SPAC27F1.04c |nuf2||spindle pole body protein
Nuf2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 441
Score = 26.2 bits (55), Expect = 5.8
Identities = 16/71 (22%), Positives = 36/71 (50%)
Frame = -1
Query: 644 EKHITLDKSMKGTDHVCSLTPSEFQQLVRDVRVIEASLGTPIKKVVTSEIPCIDKLQKSL 465
E++ + +S+ +CS +E+ ++ + ++ + ++ S + I+KLQ S+
Sbjct: 181 ERNEEMFQSLIEDKRLCSQVRTEYDRIRMEASELKIRYHN-VDSLMASTLEEIEKLQSSI 239
Query: 464 VMGSTKNKGEI 432
V K KG+I
Sbjct: 240 VHSPEKLKGKI 250
>SPBC1709.06 |dus2||tRNA dihydrouridine synthase Dus2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 479
Score = 25.8 bits (54), Expect = 7.7
Identities = 12/30 (40%), Positives = 19/30 (63%), Gaps = 2/30 (6%)
Frame = -1
Query: 566 LVRDVRVIEASL--GTPIKKVVTSEIPCID 483
LV +++ +L GTP+++VV I CID
Sbjct: 34 LVWGPEIVDKALLSGTPVERVVNDRINCID 63
>SPAC4F8.13c |rng2||IQGAP|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1489
Score = 25.8 bits (54), Expect = 7.7
Identities = 12/37 (32%), Positives = 18/37 (48%)
Frame = +1
Query: 85 NLQYILCAIQKYIISKMFFNNFRNIYIANNLPTIIYC 195
N+ L I + ++F +IY NLP +IYC
Sbjct: 103 NINKFLDFIHGIGLPEIFHFELTDIYEGKNLPKVIYC 139
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,317,841
Number of Sequences: 5004
Number of extensions: 65237
Number of successful extensions: 174
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 168
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 174
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 416455520
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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