Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fmgV19d08r
         (898 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC794.07 |||dihydrolipoamide S-acetyltransferase E2 |Schizosac...   128   1e-30
SPBC776.15c |||dihydrolipoamide S-succinyltransferase, e2 compon...   125   7e-30
SPCC1259.09c |||pyruvate dehydrogenase protein x component|Schiz...    34   0.024
SPBC83.03c |tas3||RITS complex subunit 3 |Schizosaccharomyces po...    27   3.6  
SPCC736.04c |gma12||alpha-1,2-galactosyltransferase Gma12 |Schiz...    27   3.6  
SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal protein...    26   6.3  
SPAC6F6.03c |||ribosome export GTPase|Schizosaccharomyces pombe|...    26   8.4  
SPBC36B7.09 |gcn2|ppk28, ppk28, SPBP18G5.01|eIF2 alpha kinase Gc...    26   8.4  

>SPCC794.07 |||dihydrolipoamide S-acetyltransferase E2
            |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 483

 Score =  128 bits (308), Expect = 1e-30
 Identities = 88/291 (30%), Positives = 152/291 (52%), Gaps = 4/291 (1%)
 Frame = -2

Query: 897  DLSAVKATGRNGRVLKEDMLSHLNINSDD-SNEVVLPGSVRAVEIPVTKAQARVELALED 721
            DLS ++ +G NGR++K D+ +   + +   SNE     + +A     + A A      ED
Sbjct: 202  DLSQIRGSGPNGRIIKVDIENFKPVVAPKPSNEA----AAKATTPAASAADAAAPGDYED 257

Query: 720  KVVPVTGFQRAMVKSMTEAMKI-PHFVFSDEYNVTKLVESRAALKKQAEERGVRLTYMPI 544
              +P++  ++ +   + E+  + PH+  +   N+ K++  RAAL   A+ R  +L+   +
Sbjct: 258  --LPLSNMRKIIASRLAESKNMNPHYYVTVSVNMEKIIRLRAALNAMADGR-YKLSVNDL 314

Query: 543  IIKAASLSLSYLPILNSSLDSSCENLIYKASHNIGVAMDTPNGLVVPVIKNIQIKSILEI 364
            +IKA + +L  +P +N++         YK + +I +A+ TP+GL+ PVI+N     + EI
Sbjct: 315  VIKATTAALRQVPEVNAAWMGDFIRQ-YK-NVDISMAVATPSGLITPVIRNTHALGLAEI 372

Query: 363  AQELNTLQEKGSKGQLGLNDLTGGTFTISNIGIVGGTYTKPVIFPPQVAIGALGKI--QV 190
            +       ++    +L   +  GGTFTISN+G+        +I PPQ  I A+G     V
Sbjct: 373  STLAKDYGQRARNNKLKPEEYQGGTFTISNLGMFPVDQFTAIINPPQACILAVGTTVDTV 432

Query: 189  LPRFDSQGNVVKAHILSVSWSADHRVIDGVTMARFSNHLKNYLEDPYILLL 37
            +P   S+     A I+  + S+DHRV+DG   ARF+  LK  LE+P  ++L
Sbjct: 433  VPDSTSEKGFKVAPIMKCTLSSDHRVVDGAMAARFTTALKKILENPLEIML 483


>SPBC776.15c |||dihydrolipoamide S-succinyltransferase, e2 component
           of oxoglutarate dehydrogenase complex
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 452

 Score =  125 bits (302), Expect = 7e-30
 Identities = 70/203 (34%), Positives = 114/203 (56%), Gaps = 3/203 (1%)
 Frame = -2

Query: 636 DEYNVTKLVESRAALKKQA-EERGVRLTYMPIIIKAASLSLSYLPILNSSLDSSC--ENL 466
           +E +++ +V  R   K +  +E GV++ +M    KA + ++  +P +N S++     + L
Sbjct: 250 NECDMSAVVALRKKYKDEILKETGVKIGFMSFFSKACTQAMKQIPAINGSIEGEGKGDTL 309

Query: 465 IYKASHNIGVAMDTPNGLVVPVIKNIQIKSILEIAQELNTLQEKGSKGQLGLNDLTGGTF 286
           +Y+   ++ +A+ TP GLV PVI+N +  S+LEI   + TL  K   G+L + D+  GTF
Sbjct: 310 VYRDFCDLSIAVATPKGLVTPVIRNAESMSLLEIESAIATLGSKARAGKLAIEDMASGTF 369

Query: 285 TISNIGIVGGTYTKPVIFPPQVAIGALGKIQVLPRFDSQGNVVKAHILSVSWSADHRVID 106
           TISN GI G  Y  P+I  PQ A+  L  I+  P     G VV   ++ ++ + DHR++D
Sbjct: 370 TISNGGIFGSLYGTPIINLPQTAVLGLHAIKERP-VVINGQVVPRPMMYLALTYDHRMVD 428

Query: 105 GVTMARFSNHLKNYLEDPYILLL 37
           G     F   +K Y+EDP  +LL
Sbjct: 429 GREAVTFLRLVKEYIEDPAKMLL 451


>SPCC1259.09c |||pyruvate dehydrogenase protein x
           component|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 456

 Score = 34.3 bits (75), Expect = 0.024
 Identities = 33/124 (26%), Positives = 60/124 (48%), Gaps = 1/124 (0%)
 Frame = -2

Query: 888 AVKATGRNGRVLKEDMLSHLNINSDDSNEVVLPGSVRAVEIPVTKAQARVELALEDKVVP 709
           ++ ATG +GR+LK D+L+H+    D      L   VR +E+         + +  +  VP
Sbjct: 187 SIPATGPHGRLLKGDVLAHVG-KIDKGVPSSLQKFVRNLEVLDFSGVEPKKPSYTEDSVP 245

Query: 708 VTGFQRAMVKSM-TEAMKIPHFVFSDEYNVTKLVESRAALKKQAEERGVRLTYMPIIIKA 532
           V   +  M++++ T  MK  +  F    ++ KL +S  A +K+ +  G+      +I  A
Sbjct: 246 VR--KSTMIETLKTVPMKDEYITFERSISLEKL-KSLLAKRKEKDAFGIDEVVSAMIGDA 302

Query: 531 ASLS 520
            S+S
Sbjct: 303 LSIS 306


>SPBC83.03c |tas3||RITS complex subunit 3 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 549

 Score = 27.1 bits (57), Expect = 3.6
 Identities = 18/71 (25%), Positives = 35/71 (49%), Gaps = 2/71 (2%)
 Frame = -3

Query: 650 TLYSVTSTTSQNWLNRVQHSKSKRKNVGLD*LTCRSS--SKRHL*V*AIYPFSTVVSIAP 477
           +L  V    +++WL+ +++ + KR+ +  + + CRS   S+R L     +P     S A 
Sbjct: 35  SLERVHELFTEHWLSNLKNRREKRQELAEEAVYCRSEMLSQRKLLAAVDFPQQLKNSPAK 94

Query: 476 VKISFTKQVIT 444
            K + T   +T
Sbjct: 95  AKATHTSSGVT 105


>SPCC736.04c |gma12||alpha-1,2-galactosyltransferase Gma12
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 375

 Score = 27.1 bits (57), Expect = 3.6
 Identities = 16/58 (27%), Positives = 27/58 (46%)
 Frame = +2

Query: 632 SSLNTKCGIFMASVIDLTIALWKPVTGTTLSSRANSTLACAFVTGISTALTLPGNTTS 805
           S+++TK    M  +ID   + W PV  +  S++ + TL       +S   TL    T+
Sbjct: 25  SAMDTKLQTKMKEIIDQHTSTWTPVVSSVTSTQTD-TLRVTISEVVSVTATLTETFTA 81


>SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal
           protein|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 670

 Score = 26.2 bits (55), Expect = 6.3
 Identities = 15/39 (38%), Positives = 22/39 (56%)
 Frame = +2

Query: 713 TTLSSRANSTLACAFVTGISTALTLPGNTTSFESSELMF 829
           T  SS+ NS  A A  +  STA+T+  N+T + S+   F
Sbjct: 132 TFTSSQTNSGHASASTSIPSTAITVTANSTIYSSATSSF 170


>SPAC6F6.03c |||ribosome export GTPase|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 537

 Score = 25.8 bits (54), Expect = 8.4
 Identities = 20/59 (33%), Positives = 27/59 (45%), Gaps = 1/59 (1%)
 Frame = -2

Query: 210 ALGKIQVLPRFDSQGNVVKAHIL-SVSWSADHRVIDGVTMARFSNHLKNYLEDPYILLL 37
           A G++     F S   V KA I     W  + RVI   T+ +F   +   L DPY +LL
Sbjct: 53  AAGELVRAAEFQSS-EVPKARIQPDRRWFNNTRVIAQPTLTQFREAMGQKLNDPYQVLL 110


>SPBC36B7.09 |gcn2|ppk28, ppk28, SPBP18G5.01|eIF2 alpha kinase Gcn2
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1576

 Score = 25.8 bits (54), Expect = 8.4
 Identities = 17/60 (28%), Positives = 31/60 (51%)
 Frame = +2

Query: 566 TPRSSACFLSAARDSTSFVTLYSSLNTKCGIFMASVIDLTIALWKPVTGTTLSSRANSTL 745
           TP+SS+   + ++++TS  TL +S+   C I +    D  +   + +   T+S    STL
Sbjct: 190 TPQSSSKKKTNSKETTSLETLPTSIYFDCSISVRDCHDSLVTFNRVLPLYTISHSNLSTL 249


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,633,824
Number of Sequences: 5004
Number of extensions: 76983
Number of successful extensions: 196
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 184
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 192
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 452494940
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)