Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fmgV18n04f
(715 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC17G9.11c |pyr1||pyruvate carboxylase|Schizosaccharomyces pom... 164 1e-41
SPAC56E4.04c |cut6||acetyl-CoA carboxylase|Schizosaccharomyces p... 86 5e-18
SPBC1709.08 |cft1||cleavage factor one Cft1 |Schizosaccharomyces... 31 0.22
SPBC405.01 |ade1|min4, SPBC4C3.02c|phosphoribosylamine-glycine l... 31 0.22
SPBC342.02 |||glutaminyl-tRNA synthetase |Schizosaccharomyces po... 30 0.38
SPCC1450.11c |cek1||serine/threonine protein kinase Cek1|Schizos... 29 0.87
SPAC22G7.06c |ura1||carbamoyl-phosphate synthase |Schizosaccharo... 28 1.5
SPCC162.08c |nup211||nuclear pore complex associated protein|Sch... 27 3.5
SPAC688.08 |srb8|med12|mediator complex subunit Srb8 |Schizosacc... 26 6.1
SPAC24H6.12c |uba3||NEDD8 activating enzyme|Schizosaccharomyces ... 26 6.1
SPAC6F6.04c |||membrane transporter |Schizosaccharomyces pombe|c... 26 6.1
SPBC1773.14 |arg7||argininosuccinate lyase |Schizosaccharomyces ... 25 8.1
SPAC27D7.11c |||But2 family protein|Schizosaccharomyces pombe|ch... 25 8.1
SPAC27D7.10c |||But2 family protein|Schizosaccharomyces pombe|ch... 25 8.1
SPAC140.02 |gar2||GAR family|Schizosaccharomyces pombe|chr 1|||M... 25 8.1
SPAPB18E9.02c |ppk18||serine/threonine protein kinase Ppk18 |Sch... 25 8.1
SPBC1709.13c |||lysine methyltransferase |Schizosaccharomyces po... 25 8.1
SPBP22H7.05c |||ATPase with bromodomain protein|Schizosaccharomy... 25 8.1
SPBC428.11 |||homocysteine synthase |Schizosaccharomyces pombe|c... 25 8.1
SPCC1223.14 ||SPCC297.01|chorismate synthase |Schizosaccharomyce... 25 8.1
>SPBC17G9.11c |pyr1||pyruvate carboxylase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1185
Score = 164 bits (398), Expect = 1e-41
Identities = 83/209 (39%), Positives = 122/209 (58%), Gaps = 3/209 (1%)
Frame = +1
Query: 94 NNIRYNHAQIKEKVQRTQISKVLIANRGEIACRVMRTAKKLGVRTVAVYSDADRHAMHVE 273
N ++ Q + + + +KVL+ANR EIA RV RTA +L + TVA+YS DR +MH +
Sbjct: 15 NTNPFSKLQDRSSLLGEKFTKVLVANRSEIAIRVFRTAHELSMHTVAIYSYEDRLSMHRQ 74
Query: 274 MADEAYHIGPAPSTQ---SYLNASKILEVAKKSNSQAIHPGYGFLSENVEFCEKCASEDI 444
ADE+Y IG +YL +I+ +AK++ + +HPGYGFLSEN EF K +
Sbjct: 75 KADESYPIGKVGQYSPVGAYLAIDEIVSIAKRTGANLVHPGYGFLSENAEFARKVNEAGM 134
Query: 445 IFIGPPPSAIRDMGIKSTSKAIMSAAGVPIVRGYHGEDQNIERLKAEAQRIGFPLMIKAV 624
F+GP P I +G K+ ++AI GVP+V G G ++ E +A + G P++IKA
Sbjct: 135 QFVGPSPEVIDSLGDKTKARAIAIRCGVPVVPGTPGPVEHYEEAEAFVKEYGLPVIIKAA 194
Query: 625 RGGGGKGMRIAMTESDFLPQLESAKRESL 711
GGGG+GMR+ + E A+ E+L
Sbjct: 195 MGGGGRGMRVVRSADTLKESFERARSEAL 223
>SPAC56E4.04c |cut6||acetyl-CoA carboxylase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2280
Score = 85.8 bits (203), Expect = 5e-18
Identities = 68/214 (31%), Positives = 107/214 (50%), Gaps = 39/214 (18%)
Frame = +1
Query: 142 TQISKVLIANRGEIACRVMRTAKKLGVRT-----------VAVYSDADRHAMHVEMADEA 288
T I+ +LIAN G A + +R+ +K T +A D +A ++ MAD+
Sbjct: 67 TVITSILIANNGIAAVKEIRSIRKWAYETFNNERAIKFTVMATPDDLKVNADYIRMADQY 126
Query: 289 YHIGPAPSTQSYLNASKILEVAKKSNSQAIHPGYGFLSENVEFCE--KCASEDIIFIGPP 462
+ + +Y N I+++A++ N A+ G+G SEN + E +S+ I+FIGPP
Sbjct: 127 VEVPGGSNNNNYANVELIVDIAERMNVHAVWAGWGHASENPKLPEMLSASSKKIVFIGPP 186
Query: 463 PSAIRDMGIKSTSKAIMSAAGVPIVRGYHG---------EDQNI---------------- 567
SA+R +G K +S + +A VP + + G E+ NI
Sbjct: 187 GSAMRSLGDKISSTIVAQSARVPCM-SWSGNELDQVRIDEETNIVTVDDDVYQKACIRSA 245
Query: 568 ERLKAEAQRIGFPLMIKAVRGGGGKGMR-IAMTE 666
E A A++IG+P+MIKA GGGGKG+R + TE
Sbjct: 246 EEGIAVAEKIGYPVMIKASEGGGGKGIRQVTSTE 279
>SPBC1709.08 |cft1||cleavage factor one Cft1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1441
Score = 30.7 bits (66), Expect = 0.22
Identities = 16/40 (40%), Positives = 21/40 (52%)
Frame = -2
Query: 219 SQFLCSSHDPTCYLPSVCYQYFADLCSLNFLFDLCMVVPY 100
S +CSSH PT L +D C L FL D+ ++PY
Sbjct: 155 SSNICSSHTPTQLLVDPD----SDCCLLRFLTDMMAIIPY 190
>SPBC405.01 |ade1|min4, SPBC4C3.02c|phosphoribosylamine-glycine
ligase |Schizosaccharomyces pombe|chr 2|||Manual
Length = 788
Score = 30.7 bits (66), Expect = 0.22
Identities = 24/80 (30%), Positives = 33/80 (41%)
Frame = +1
Query: 454 GPPPSAIRDMGIKSTSKAIMSAAGVPIVRGYHGEDQNIERLKAEAQRIGFPLMIKAVRGG 633
GP A R G K SK M +P + N + K+ F ++IKA
Sbjct: 99 GPSALAARMEGSKVFSKDFMHRNNIPTA--VYKSFSNYDHAKSFLDTCTFDVVIKADGLA 156
Query: 634 GGKGMRIAMTESDFLPQLES 693
GKG+ I T+ + LES
Sbjct: 157 AGKGVIIPKTKKEAFEALES 176
>SPBC342.02 |||glutaminyl-tRNA synthetase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 811
Score = 29.9 bits (64), Expect = 0.38
Identities = 12/32 (37%), Positives = 22/32 (68%)
Frame = +2
Query: 536 LEDTMVKIRTLRD*KLKPKELVSRL*SRLSGG 631
+E+++++ R +RD K +PKE + R+ LS G
Sbjct: 386 IEESLLEFRNMRDGKYQPKEAILRMKQDLSDG 417
>SPCC1450.11c |cek1||serine/threonine protein kinase
Cek1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1338
Score = 28.7 bits (61), Expect = 0.87
Identities = 18/69 (26%), Positives = 32/69 (46%)
Frame = +1
Query: 184 ACRVMRTAKKLGVRTVAVYSDADRHAMHVEMADEAYHIGPAPSTQSYLNASKILEVAKKS 363
A ++ + L + ++ S RH HV +++ + IG +P +Q N S L K S
Sbjct: 451 ATNTPQSDRSLDINDLSRSSSYSRHLSHVSLSNPDFAIG-SPMSQDSSNYSSPLHRRKAS 509
Query: 364 NSQAIHPGY 390
+S P +
Sbjct: 510 DSNFSDPRF 518
>SPAC22G7.06c |ura1||carbamoyl-phosphate synthase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2244
Score = 27.9 bits (59), Expect = 1.5
Identities = 31/171 (18%), Positives = 66/171 (38%)
Frame = +1
Query: 184 ACRVMRTAKKLGVRTVAVYSDADRHAMHVEMADEAYHIGPAPSTQSYLNASKILEVAKKS 363
A R +RT + GV+T+ V + + + + AD Y + +L++ ++
Sbjct: 1036 AVRAVRTLRDRGVKTIMVNYNPETVSTDYDEADRLYF--------ENIGLETVLDIYEQE 1087
Query: 364 NSQAIHPGYGFLSENVEFCEKCASEDIIFIGPPPSAIRDMGIKSTSKAIMSAAGVPIVRG 543
+S I G + N E++ +G P I G ++ K + + +
Sbjct: 1088 SSSGIIIAMGGQTAN-NIALPLHRENVKILGTSPEMID--GAENRFKFSRMLDDIGVDQP 1144
Query: 544 YHGEDQNIERLKAEAQRIGFPLMIKAVRGGGGKGMRIAMTESDFLPQLESA 696
E + + +G+P++++ G M ++SD L+ A
Sbjct: 1145 KWKELTSFDEADKFCDTVGYPVLVRPSYVLSGAAMNTVYSQSDLHSYLQQA 1195
>SPCC162.08c |nup211||nuclear pore complex associated
protein|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1837
Score = 26.6 bits (56), Expect = 3.5
Identities = 14/48 (29%), Positives = 28/48 (58%)
Frame = +1
Query: 16 IAKKRLTLKMHYLRYLYRNSPLTTLQNNIRYNHAQIKEKVQRTQISKV 159
I R+ +++Y + L RNS LQ+N RY+ + + K+ ++S++
Sbjct: 51 IKNDRIVTEVNYEQQL-RNSEKKLLQSNERYDLLEDERKLLENELSQI 97
>SPAC688.08 |srb8|med12|mediator complex subunit Srb8
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1233
Score = 25.8 bits (54), Expect = 6.1
Identities = 12/29 (41%), Positives = 18/29 (62%), Gaps = 1/29 (3%)
Frame = -3
Query: 698 FADSNCGRKSDSVI-AILIPFPPPPRTAL 615
++ + GR++D+VI PF PPPR L
Sbjct: 101 YSSTLLGRQNDAVIDEPFAPFRPPPRVTL 129
>SPAC24H6.12c |uba3||NEDD8 activating enzyme|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 444
Score = 25.8 bits (54), Expect = 6.1
Identities = 19/99 (19%), Positives = 43/99 (43%), Gaps = 1/99 (1%)
Frame = +1
Query: 124 KEKVQRTQISKVLIANRGEIACRVMRTAKKLGVRTVAVYSDADRHAMHVEMADEAYHIGP 303
+E ++ SK+LI G + C +++ G R ++V D + + + +
Sbjct: 36 EETLKSAFSSKILIIGAGGLGCEILKDLALSGFRDLSV---IDMDTIDITNLNRQFLFNE 92
Query: 304 APSTQSYLNASKILEVAKKSNSQAIHPGYGFLSE-NVEF 417
+ + N + + + K+ S + P YG + + +EF
Sbjct: 93 SNIDEPKANVAASM-IMKRIPSTVVTPFYGKIQDKTIEF 130
>SPAC6F6.04c |||membrane transporter |Schizosaccharomyces pombe|chr
1|||Manual
Length = 489
Score = 25.8 bits (54), Expect = 6.1
Identities = 12/28 (42%), Positives = 17/28 (60%)
Frame = +1
Query: 280 DEAYHIGPAPSTQSYLNASKILEVAKKS 363
D++Y G PST +Y+N E+ KKS
Sbjct: 454 DKSYIDGVMPSTDTYMNEKTSDELDKKS 481
>SPBC1773.14 |arg7||argininosuccinate lyase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 461
Score = 25.4 bits (53), Expect = 8.1
Identities = 13/33 (39%), Positives = 19/33 (57%)
Frame = +2
Query: 200 ELQRNWESGQWLFILMPTDMLCMLKWQMRLTTL 298
++QR W SGQ F+L P+D + RLT +
Sbjct: 70 QIQREWNSGQ--FVLEPSDEDVHTANERRLTEI 100
>SPAC27D7.11c |||But2 family protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 463
Score = 25.4 bits (53), Expect = 8.1
Identities = 12/29 (41%), Positives = 14/29 (48%)
Frame = -2
Query: 303 RPNVVSLICHFNMHSMSVGIRINSHCPDS 217
RPN ++ I FN S N CPDS
Sbjct: 402 RPNRIAEIGRFNCSSSGCDYATNVTCPDS 430
>SPAC27D7.10c |||But2 family protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 383
Score = 25.4 bits (53), Expect = 8.1
Identities = 12/29 (41%), Positives = 14/29 (48%)
Frame = -2
Query: 303 RPNVVSLICHFNMHSMSVGIRINSHCPDS 217
RPN ++ I FN S N CPDS
Sbjct: 322 RPNRIAEIGRFNCSSSGCDYATNVTCPDS 350
>SPAC140.02 |gar2||GAR family|Schizosaccharomyces pombe|chr
1|||Manual
Length = 500
Score = 25.4 bits (53), Expect = 8.1
Identities = 12/33 (36%), Positives = 19/33 (57%)
Frame = +3
Query: 129 ESSKNTDQQSIDSKQRGDSMSGHENCKEIGSQD 227
ESS +++ S S + GDS S ++ E S+D
Sbjct: 203 ESSSDSESSSDSSSESGDSDSSSDSESESSSED 235
>SPAPB18E9.02c |ppk18||serine/threonine protein kinase Ppk18
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1316
Score = 25.4 bits (53), Expect = 8.1
Identities = 11/30 (36%), Positives = 15/30 (50%)
Frame = +1
Query: 457 PPPSAIRDMGIKSTSKAIMSAAGVPIVRGY 546
P P R +GI + S I S G P ++ Y
Sbjct: 535 PKPFLSRQIGISTLSSNISSGKGTPSIQDY 564
>SPBC1709.13c |||lysine methyltransferase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 547
Score = 25.4 bits (53), Expect = 8.1
Identities = 10/28 (35%), Positives = 19/28 (67%)
Frame = +1
Query: 61 LYRNSPLTTLQNNIRYNHAQIKEKVQRT 144
+YR+S + LQ++I Y ++E +Q+T
Sbjct: 378 IYRDSQINILQDSITYVKNYMEESLQKT 405
>SPBP22H7.05c |||ATPase with bromodomain protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1201
Score = 25.4 bits (53), Expect = 8.1
Identities = 10/30 (33%), Positives = 16/30 (53%)
Frame = -2
Query: 675 EIRFGHCDSHTLSSAPPDSLDHKRETNSLG 586
++ FGH D T+ S P + + NS+G
Sbjct: 346 QLHFGHIDEKTIRSTDPFANRENLDFNSIG 375
>SPBC428.11 |||homocysteine synthase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 429
Score = 25.4 bits (53), Expect = 8.1
Identities = 11/26 (42%), Positives = 14/26 (53%)
Frame = +1
Query: 562 NIERLKAEAQRIGFPLMIKAVRGGGG 639
+ ER+ A G PLM+ GGGG
Sbjct: 166 DFERIAEVAHAAGVPLMVDNTFGGGG 191
>SPCC1223.14 ||SPCC297.01|chorismate synthase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 395
Score = 25.4 bits (53), Expect = 8.1
Identities = 12/45 (26%), Positives = 22/45 (48%)
Frame = +3
Query: 108 QPCTNQRESSKNTDQQSIDSKQRGDSMSGHENCKEIGSQDSGCLF 242
+PC ++ E+ SI + + + SG E CK GS+ + +
Sbjct: 248 EPCFDKLEAKLAHAMMSIPATKSFEIGSGREGCKVAGSKHNDLFY 292
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,975,477
Number of Sequences: 5004
Number of extensions: 63171
Number of successful extensions: 224
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 215
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 221
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 333194204
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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