BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fmgV18e23f
(692 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC342.02 |||glutaminyl-tRNA synthetase |Schizosaccharomyces po... 27 3.4
SPAC869.05c |||sulfate transporter |Schizosaccharomyces pombe|ch... 26 4.5
SPBP8B7.23 |||ubiquitin-protein ligase E3 |Schizosaccharomyces p... 26 4.5
SPAC644.11c |||pyruvate dehydrogenase |Schizosaccharomyces pombe... 26 5.9
SPBC21D10.11c |nfs1||iron-sulfur cluster assembly protein Nfs1|S... 26 5.9
SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal protein... 25 7.9
>SPBC342.02 |||glutaminyl-tRNA synthetase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 811
Score = 26.6 bits (56), Expect = 3.4
Identities = 18/85 (21%), Positives = 35/85 (41%), Gaps = 1/85 (1%)
Frame = +3
Query: 174 PGGHSALFSKRARRILRQPG*S*IYRFSITSTW*FHIPHFSKLWRCTCLSYPQHSGQTCR 353
PGG++ L +R + L+ G + RF HI H + + ++ C
Sbjct: 241 PGGNTQLIPERMKEHLQATGGGVVTRFPPEPNGYLHIGHSKAI--AVNFGFARYHNGVCY 298
Query: 354 RRYPESQPH-QQTVHSEISTGLLQW 425
R+ ++ P ++ + E L+ W
Sbjct: 299 LRFDDTNPEAEEERYFESIKDLVAW 323
>SPAC869.05c |||sulfate transporter |Schizosaccharomyces pombe|chr
1|||Manual
Length = 840
Score = 26.2 bits (55), Expect = 4.5
Identities = 14/42 (33%), Positives = 21/42 (50%)
Frame = +1
Query: 244 SIGFQSPVPGDFIYRTSLNSGGVPASPILNIADRLVADAIRR 369
++ QSP PG FI+R + AS + + R + D RR
Sbjct: 578 NLEIQSPPPGVFIFRLQESFTYPNASRVSTMISRRIKDLTRR 619
>SPBP8B7.23 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 673
Score = 26.2 bits (55), Expect = 4.5
Identities = 16/62 (25%), Positives = 33/62 (53%), Gaps = 4/62 (6%)
Frame = +1
Query: 31 ESTGQTTQARSSYTTSEIMWGHRFVPLVTYNRERQ----GYQVDYSKFDETTQVDTPLCS 198
EST + S+ +T++ +WG R + + + + Q G++VD+ D+ Q+ P +
Sbjct: 598 ESTDEVNTDESTLSTAKTVWGTRALANIQNDTDDQDDLDGWKVDW---DKVAQLSAPSKN 654
Query: 199 AK 204
+K
Sbjct: 655 SK 656
>SPAC644.11c |||pyruvate dehydrogenase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 425
Score = 25.8 bits (54), Expect = 5.9
Identities = 14/27 (51%), Positives = 16/27 (59%)
Frame = -1
Query: 407 GYFTVDGLLVRLALRIASATSLSAMLR 327
G F D L +RLA RI +LS MLR
Sbjct: 44 GLFLRDELPIRLARRIQDLQNLSPMLR 70
>SPBC21D10.11c |nfs1||iron-sulfur cluster assembly protein
Nfs1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 498
Score = 25.8 bits (54), Expect = 5.9
Identities = 17/72 (23%), Positives = 30/72 (41%)
Frame = +1
Query: 121 NRERQGYQVDYSKFDETTQVDTPLCSAKELDEFYGSQADRRSIGFQSPVPGDFIYRTSLN 300
N RQ V F ++++D P S K ++ YG Q P+P + + +L+
Sbjct: 30 NNVRQSLGVYGRSFMTSSRMDKPSMSNKPREQMYGL---GNMTAVQEPIPENSLKTVTLD 86
Query: 301 SGGVPASPILNI 336
AS + +
Sbjct: 87 QAQTAASTVTGL 98
>SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 670
Score = 25.4 bits (53), Expect = 7.9
Identities = 10/26 (38%), Positives = 17/26 (65%)
Frame = +1
Query: 19 EGTIESTGQTTQARSSYTTSEIMWGH 96
EG +TG T Q+ +++T+S+ GH
Sbjct: 117 EGISSTTGSTFQSMTTFTSSQTNSGH 142
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,677,593
Number of Sequences: 5004
Number of extensions: 51145
Number of successful extensions: 132
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 129
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 132
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 321951680
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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