Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fmgV18e05r
(923 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 374 e-104
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 374 e-104
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 374 e-104
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 114 1e-26
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 85 1e-17
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 75 2e-14
SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 29 1.2
SPBC21D10.06c |map4||cell agglutination protein Map4|Schizosacch... 27 3.7
SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subuni... 27 4.9
SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal protein... 26 8.6
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 374 bits (920), Expect = e-104
Identities = 171/217 (78%), Positives = 193/217 (88%)
Frame = -3
Query: 912 GKCLIEALDAILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPAN 733
GK L+EA+D+I PPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGV+KPG IV FAPA
Sbjct: 223 GKTLLEAIDSIEPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVIKPGMIVTFAPAG 282
Query: 732 ITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQV 553
+TTEVKSVEMHHE+L +PGDNVGFNVKNVSVK++RRG V GDSKN+PP G A FTAQV
Sbjct: 283 VTTEVKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQV 342
Query: 552 IVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNL 373
I+LNHPGQIS GY+PVLDCHTAHIACKFAE+ EK+DRR+GK E +PK +KSGDA I +
Sbjct: 343 IILNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKM 402
Query: 372 VPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAV 262
VPSKP+CVE+F ++ PLGRFAVRDMRQTVAVGVIKAV
Sbjct: 403 VPSKPMCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAV 439
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 374 bits (920), Expect = e-104
Identities = 171/217 (78%), Positives = 193/217 (88%)
Frame = -3
Query: 912 GKCLIEALDAILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPAN 733
GK L+EA+D+I PPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGV+KPG IV FAPA
Sbjct: 223 GKTLLEAIDSIEPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVIKPGMIVTFAPAG 282
Query: 732 ITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQV 553
+TTEVKSVEMHHE+L +PGDNVGFNVKNVSVK++RRG V GDSKN+PP G A FTAQV
Sbjct: 283 VTTEVKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQV 342
Query: 552 IVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNL 373
I+LNHPGQIS GY+PVLDCHTAHIACKFAE+ EK+DRR+GK E +PK +KSGDA I +
Sbjct: 343 IILNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKM 402
Query: 372 VPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAV 262
VPSKP+CVE+F ++ PLGRFAVRDMRQTVAVGVIKAV
Sbjct: 403 VPSKPMCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAV 439
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 374 bits (920), Expect = e-104
Identities = 171/217 (78%), Positives = 193/217 (88%)
Frame = -3
Query: 912 GKCLIEALDAILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPAN 733
GK L+EA+D+I PPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGV+KPG IV FAPA
Sbjct: 223 GKTLLEAIDSIEPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVIKPGMIVTFAPAG 282
Query: 732 ITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQV 553
+TTEVKSVEMHHE+L +PGDNVGFNVKNVSVK++RRG V GDSKN+PP G A FTAQV
Sbjct: 283 VTTEVKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQV 342
Query: 552 IVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNL 373
I+LNHPGQIS GY+PVLDCHTAHIACKFAE+ EK+DRR+GK E +PK +KSGDA I +
Sbjct: 343 IILNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKM 402
Query: 372 VPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAV 262
VPSKP+CVE+F ++ PLGRFAVRDMRQTVAVGVIKAV
Sbjct: 403 VPSKPMCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAV 439
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 114 bits (275), Expect = 1e-26
Identities = 69/220 (31%), Positives = 116/220 (52%), Gaps = 2/220 (0%)
Frame = -3
Query: 912 GKCLIEALDAILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPAN 733
G L+E LD++ R + P +P+ YK +GT+ G++E G +K + V+ P N
Sbjct: 448 GPSLLEYLDSMTHLERKVNAPFIMPIASKYK--DLGTILEGKIEAGSIKKNSNVLVMPIN 505
Query: 732 ITTEVKSV-EMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQ 556
T EV ++ + E + ++ GD V V+ +++ GYV +KN P F AQ
Sbjct: 506 QTLEVTAIYDEADEEISSSICGDQVRLRVRGDD-SDVQTGYVLTSTKN-PVHATTRFIAQ 563
Query: 555 VIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVN 376
+ +L P ++ GY+ V+ HTA FA++ K+D+ T + ++ P G I
Sbjct: 564 IAILELPSILTTGYSCVMHIHTAVEEVSFAKLLHKLDK-TNRKSKKPPMFATKGMKIIAE 622
Query: 375 LVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVG-VIKAVN 259
L P+C+E F+++ +GRF +RD TVAVG V+K ++
Sbjct: 623 LETQTPVCMERFEDYQYMGRFTLRDQGTTVAVGKVVKILD 662
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 85.0 bits (201), Expect = 1e-17
Identities = 67/216 (31%), Positives = 103/216 (47%), Gaps = 1/216 (0%)
Frame = -3
Query: 912 GKCLIEALDAILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPAN 733
G L+ ALD ++PP +P KPLRL + DVY+ TV GRVE G ++ ++ +
Sbjct: 384 GPTLLSALDQLVPPEKPYRKPLRLSIDDVYRSPRSVTV-TGRVEAGNVQVNQVLYDVSSQ 442
Query: 732 ITTEVKSVEMHHEALQE-AVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQ 556
VK+V + + AV GD V + ++ V +LR G + + + NP + F A+
Sbjct: 443 EDAYVKNVIRNSDPSSTWAVAGDTVTLQLADIEVNQLRPGDILSNYE-NPVRRVRSFVAE 501
Query: 555 VIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVN 376
+ + G I +G T VL H+ + K+ K + + S K I
Sbjct: 502 IQTFDIHGPILSGSTLVL-----HLGRTVTSVSLKIVTVNNKRSR-HIASRKRALVRISF 555
Query: 375 LVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIK 268
L PLC+ +E P LGRF +R TVA G++K
Sbjct: 556 LDGLFPLCLA--EECPALGRFILRRSGDTVAAGIVK 589
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 74.5 bits (175), Expect = 2e-14
Identities = 58/214 (27%), Positives = 99/214 (46%), Gaps = 3/214 (1%)
Frame = -3
Query: 903 LIEALDAILP-PARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGT--IVVFAPAN 733
L+EA+D+ + P R TD P + ++DV+ I G GTV GRVE G LK G +V ++
Sbjct: 234 LMEAVDSYITLPERKTDVPFLMAIEDVFSISGRGTVVTGRVERGTLKKGAEIEIVGYGSH 293
Query: 732 ITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQV 553
+ T V +EM + L AV GDN G ++++ ++L+RG + P F A
Sbjct: 294 LKTTVTGIEMFKKQLDAAVAGDNCGLLLRSIKREQLKRGMIVAQPGTVAPH--QKFKASF 351
Query: 552 IVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNL 373
+L + T +D + + + +++ ++ T + K + GD +
Sbjct: 352 YILTK--EEGGRRTGFVDKYRPQLYSRTSDVTVEL---THPDPNDSDKMVMPGDNVEMIC 406
Query: 372 VPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVI 271
P+ +E Q RF VR+ TV ++
Sbjct: 407 TLIHPIVIEKGQ------RFTVREGGSTVGTALV 434
>SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 646
Score = 28.7 bits (61), Expect = 1.2
Identities = 25/93 (26%), Positives = 41/93 (44%), Gaps = 5/93 (5%)
Frame = -3
Query: 903 LIEALDAILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPANITT 724
L E + I PP + PLR L D + G + + R+ G +K G V+
Sbjct: 226 LPEIIQKIPPPKGSENAPLRCLLIDSWYNSYQGVIGLVRIMEGFIKKGGKVMSVNTGRKY 285
Query: 723 EVKSVEMHHEALQEA--VPGDNVGF---NVKNV 640
EV+ V + + + E + VG+ N+KN+
Sbjct: 286 EVQQVGIMYPDMTEVSRLRAGQVGYIIWNMKNI 318
>SPBC21D10.06c |map4||cell agglutination protein
Map4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 948
Score = 27.1 bits (57), Expect = 3.7
Identities = 14/39 (35%), Positives = 22/39 (56%)
Frame = +2
Query: 719 TSVVMLAGAKTTMVPGFNTPVSTLPTGTVPIPPILYTSC 835
+SVV+ + +T V + + VST TGTV +P +C
Sbjct: 86 SSVVLYSAKETVTVSSYWSLVSTSVTGTVYVPYTSSVAC 124
>SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subunit a
Pol2 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 2199
Score = 26.6 bits (56), Expect = 4.9
Identities = 12/25 (48%), Positives = 15/25 (60%)
Frame = +1
Query: 625 QFLDGHVLYVETYIVSRYSFLESFV 699
+F DGH+L ETY+ LES V
Sbjct: 527 KFFDGHLLASETYVGGHVESLESGV 551
>SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 670
Score = 25.8 bits (54), Expect = 8.6
Identities = 24/85 (28%), Positives = 42/85 (49%), Gaps = 2/85 (2%)
Frame = +2
Query: 218 SAALVTLPPPASLKLTALMTPTATVCLMSRTAKRPRGGNSWKDSTH--RGLEGTKLTMAA 391
S +VTLPPPAS ++ T T T + S ++ G+ + +++ + ++++
Sbjct: 183 STDIVTLPPPAS-STSSFSTITNTSMIPSSSSFTTTTGSPYYNTSSFLPSSVISSASLSS 241
Query: 392 SPDLMDFGLTSVDLPVRRSTFSLIS 466
S L +TS PV S+ SL S
Sbjct: 242 SSVLPTSIITSTSTPVTVSSSSLSS 266
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,833,930
Number of Sequences: 5004
Number of extensions: 81618
Number of successful extensions: 259
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 240
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 255
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 467341524
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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