Bombyx BLAST Result against bee

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fmgV18d21r
         (846 letters)

Database: bee 
           438 sequences; 146,343 total letters

Searching......................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

DQ026034-1|AAY87893.1|  569|Apis mellifera nicotinic acetylcholi...    23   3.5  
DQ026033-1|AAY87892.1|  569|Apis mellifera nicotinic acetylcholi...    23   3.5  
DQ325132-1|ABD14146.1|  189|Apis mellifera complementary sex det...    22   8.2  
DQ325131-1|ABD14145.1|  189|Apis mellifera complementary sex det...    22   8.2  

>DQ026034-1|AAY87893.1|  569|Apis mellifera nicotinic acetylcholine
           receptor alpha4subunit protein.
          Length = 569

 Score = 23.0 bits (47), Expect = 3.5
 Identities = 22/75 (29%), Positives = 41/75 (54%), Gaps = 2/75 (2%)
 Frame = -3

Query: 235 ISAIAILYVIPPSNC--QVFHTVS*IVNLDVFFPVVL*INTHSSL*RRV*NKYIN*KKLV 62
           IS + +L    PS+   +V  ++S +++L VFF +V+ I   +SL   +  KY+    ++
Sbjct: 258 ISFLTVLTFYLPSDSGEKVTLSISILISLHVFFLLVVEIIPPTSLVVPLLGKYLI-FAMI 316

Query: 61  CIIISCDIYELCLNV 17
            + IS  +  + LNV
Sbjct: 317 LVSISICVTVVVLNV 331


>DQ026033-1|AAY87892.1|  569|Apis mellifera nicotinic acetylcholine
           receptor alpha4subunit protein.
          Length = 569

 Score = 23.0 bits (47), Expect = 3.5
 Identities = 22/75 (29%), Positives = 41/75 (54%), Gaps = 2/75 (2%)
 Frame = -3

Query: 235 ISAIAILYVIPPSNC--QVFHTVS*IVNLDVFFPVVL*INTHSSL*RRV*NKYIN*KKLV 62
           IS + +L    PS+   +V  ++S +++L VFF +V+ I   +SL   +  KY+    ++
Sbjct: 258 ISFLTVLTFYLPSDSGEKVTLSISILISLHVFFLLVVEIIPPTSLVVPLLGKYLI-FAMI 316

Query: 61  CIIISCDIYELCLNV 17
            + IS  +  + LNV
Sbjct: 317 LVSISICVTVVVLNV 331


>DQ325132-1|ABD14146.1|  189|Apis mellifera complementary sex
           determiner protein.
          Length = 189

 Score = 21.8 bits (44), Expect = 8.2
 Identities = 7/20 (35%), Positives = 13/20 (65%)
 Frame = -2

Query: 839 SNYNNNNKTLFXPHMYRVSK 780
           +NYNNN K L+  ++  + +
Sbjct: 107 NNYNNNYKKLYKNYIINIEQ 126


>DQ325131-1|ABD14145.1|  189|Apis mellifera complementary sex
           determiner protein.
          Length = 189

 Score = 21.8 bits (44), Expect = 8.2
 Identities = 7/20 (35%), Positives = 13/20 (65%)
 Frame = -2

Query: 839 SNYNNNNKTLFXPHMYRVSK 780
           +NYNNN K L+  ++  + +
Sbjct: 107 NNYNNNYKKLYKNYIINIEQ 126


  Database: bee
    Posted date:  Oct 23, 2007  1:17 PM
  Number of letters in database: 146,343
  Number of sequences in database:  438
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 224,000
Number of Sequences: 438
Number of extensions: 4437
Number of successful extensions: 27
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 27
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 27
length of database: 146,343
effective HSP length: 57
effective length of database: 121,377
effective search space used: 27188448
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)