Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fmgV17e22f
         (742 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC688.04c |gst3||glutathione S-transferase |Schizosaccharomyce...    29   0.92 
SPAC1039.03 |||esterase/lipase |Schizosaccharomyces pombe|chr 1|...    29   0.92 
SPCC550.03c |||RNA helicase involved in mRNA catabolism|Schizosa...    27   2.1  
SPAC23D3.13c |||guanyl-nucleotide exchange factor|Schizosaccharo...    26   4.9  
SPBC21D10.09c |||ubiquitin-protein ligase E3 |Schizosaccharomyce...    26   6.5  
SPAC4A8.07c |||sphingoid long chain base |Schizosaccharomyces po...    26   6.5  

>SPAC688.04c |gst3||glutathione S-transferase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 242

 Score = 28.7 bits (61), Expect = 0.92
 Identities = 19/76 (25%), Positives = 38/76 (50%), Gaps = 1/76 (1%)
 Frame = +1

Query: 310 LYLLAELKT-ISLKVTTVDMQKPPPDFRTNFEATHPPILIDNGLAILENEKIERHIMKSV 486
           +++L ELK    +KV      + PP +         PI++D+G+  +E+  I  H+++  
Sbjct: 16  VWMLEELKVPYEIKVYDRVDGRAPPAYTKLSPLGKSPIVVDDGVTYIESAAILEHLVRKY 75

Query: 487 PGGHNLFVQDKEVASL 534
             G +    +++VA L
Sbjct: 76  --GPSFKPSEEDVAEL 89


>SPAC1039.03 |||esterase/lipase |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 341

 Score = 28.7 bits (61), Expect = 0.92
 Identities = 21/57 (36%), Positives = 25/57 (43%), Gaps = 3/57 (5%)
 Frame = -1

Query: 664 PLGTWSPCVLEVHR--CVRDELVTP-LISVHLCEPAPASVLNTGSRLETRPPCPARI 503
           P G W PC L  H    V   + T    + H+CE A   V+N   RL    P PA I
Sbjct: 96  PEGGW-PCFLWFHGGGWVLGNINTENSFATHMCEQAKCVVVNVDYRLAPEDPFPACI 151


>SPCC550.03c |||RNA helicase involved in mRNA
           catabolism|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1213

 Score = 27.5 bits (58), Expect = 2.1
 Identities = 16/51 (31%), Positives = 24/51 (47%)
 Frame = +1

Query: 517 KEVASLIENLYSKLKLVLVRKDEQKSAALRAHLGRIDGLLERRETRFLTGD 669
           KEV  LIE+L SK     +RK   K+  +R     I  L +  +   + G+
Sbjct: 101 KEVPELIEDLNSKNSSSFLRKPSSKNEFVRGSTSNIPFLADDSDVDAIAGE 151


>SPAC23D3.13c |||guanyl-nucleotide exchange
           factor|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1616

 Score = 26.2 bits (55), Expect = 4.9
 Identities = 13/39 (33%), Positives = 22/39 (56%)
 Frame = +1

Query: 454 EKIERHIMKSVPGGHNLFVQDKEVASLIENLYSKLKLVL 570
           +KIERHI  S+     L + D     ++E+L++ L  V+
Sbjct: 67  KKIERHIYISLNSIQLLAINDALSPDILESLFNSLNAVI 105


>SPBC21D10.09c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 1610

 Score = 25.8 bits (54), Expect = 6.5
 Identities = 21/82 (25%), Positives = 35/82 (42%)
 Frame = +1

Query: 400 EATHPPILIDNGLAILENEKIERHIMKSVPGGHNLFVQDKEVASLIENLYSKLKLVLVRK 579
           E T   IL       L N+ ++ H++      H+L      +    ++LYS L ++L  K
Sbjct: 690 EETIKNILFTTLEYFLTNDWVDNHLIILCASLHSLKAHLPTIFDENKSLYSLLPVILFSK 749

Query: 580 DEQKSAALRAHLGRIDGLLERR 645
            E     + AH   I  LL+ +
Sbjct: 750 PED-DGHVAAHFESIFSLLKEK 770


>SPAC4A8.07c |||sphingoid long chain base |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 458

 Score = 25.8 bits (54), Expect = 6.5
 Identities = 14/44 (31%), Positives = 23/44 (52%), Gaps = 1/44 (2%)
 Frame = +1

Query: 508 VQDKEVASLIENLYSKLKLVLVR-KDEQKSAALRAHLGRIDGLL 636
           + + E   +  + +S  ++VL R KD  KS A    +G  DG+L
Sbjct: 124 IWESEAEPVFSSAHSICEVVLTRRKDHAKSIAKNLDVGSYDGIL 167


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,037,392
Number of Sequences: 5004
Number of extensions: 62440
Number of successful extensions: 186
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 182
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 186
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 351258950
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)