Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fmgV16p08f
         (785 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC186.08c |||L-lactate dehydrogenase |Schizosaccharomyces pomb...    41   2e-04
SPAC4H3.10c |pyk1||pyruvate kinase |Schizosaccharomyces pombe|ch...    36   0.009
SPAC4G8.12c |||alpha-1,2-mannosyltransferase |Schizosaccharomyce...    29   1.00 
SPCC1902.01 |gaf1|SPCC417.01c|transcription factor Gaf1 |Schizos...    28   1.7  
SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyce...    28   1.7  
SPAC105.03c |||transcription factor |Schizosaccharomyces pombe|c...    27   4.0  
SPAC13A11.06 ||SPAC3H8.01|pyruvate decarboxylase |Schizosaccharo...    26   5.3  
SPBC1709.01 |chs2|SPBC1734.17|chitin synthase homolog Chs2|Schiz...    26   7.0  
SPAC1F8.07c |||pyruvate decarboxylase |Schizosaccharomyces pombe...    26   7.0  
SPAC31A2.05c |mis4||cohesin loading factor Mis4|Schizosaccharomy...    25   9.3  

>SPAC186.08c |||L-lactate dehydrogenase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 330

 Score = 40.7 bits (91), Expect = 2e-04
 Identities = 38/133 (28%), Positives = 53/133 (39%)
 Frame = +1

Query: 337 EGVVMELADCALPLLAGVLPTANPEEAFKDVAAAFLVGAMPRKEGMERKDLLAANVRIFK 516
           EG  M+L   A PL           +  KD  A  +     +K G  R DLL AN+ IFK
Sbjct: 59  EGEAMDLNHAA-PLSHETRVYLGDYKDCKDATAVVITAGKNQKPGETRMDLLKANISIFK 117

Query: 517 EQGQALDKVARKDVKVLVVGNPANTNALICSKYAPSIPKENFTAMTRLDQNRAQSQLAAK 696
           E  + + K   KD  +LV  NP +       K      +    + T +D  R Q  +   
Sbjct: 118 EILREVTKYT-KDAILLVATNPVDVLTYATLKLTGFPAERVIGSGTIIDTARFQYLIGKL 176

Query: 697 IGVPVKDVKRVII 735
            G+  + V   II
Sbjct: 177 YGLDPQSVNADII 189


>SPAC4H3.10c |pyk1||pyruvate kinase |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 509

 Score = 35.5 bits (78), Expect = 0.009
 Identities = 20/53 (37%), Positives = 26/53 (49%)
 Frame = +1

Query: 529 ALDKVARKDVKVLVVGNPANTNALICSKYAPSIPKENFTAMTRLDQNRAQSQL 687
           A+      D K +VV + +   A +CSKY PSIP      +TR  Q   QS L
Sbjct: 394 AIGASIESDAKAIVVLSTSGNTARLCSKYRPSIP---IVMVTRCPQRARQSHL 443



 Score = 25.4 bits (53), Expect = 9.3
 Identities = 17/58 (29%), Positives = 29/58 (50%)
 Frame = +1

Query: 532 LDKVARKDVKVLVVGNPANTNALICSKYAPSIPKENFTAMTRLDQNRAQSQLAAKIGV 705
           ++KV  K++KV V     N N  I SK   ++PK +       ++++A  +   K GV
Sbjct: 162 IEKVDDKNLKVRV-----NNNGKISSKKGVNLPKTDVDLPALSEKDKADLRFGVKNGV 214


>SPAC4G8.12c |||alpha-1,2-mannosyltransferase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 533

 Score = 28.7 bits (61), Expect = 1.00
 Identities = 37/165 (22%), Positives = 54/165 (32%), Gaps = 1/165 (0%)
 Frame = +2

Query: 5   YVVHTYIFMFYLCIKQSRNSTEFYFFKACVSSSFSF-IV*ECKTSDNTFKLA*RDNYTGI 181
           +V+  YI+    C K++ N+ +  F   C+  S SF  V  C   D  F       +   
Sbjct: 193 FVIAPYIYFSVRCFKKNVNNPKDIFLHLCIFVSVSFATVLACILEDYKFYGVFVITFWNN 252

Query: 182 SKWXXXXXXXXXXXXXXXHTHFFIKLRLEQFLDLSNLSSSTFLILRL*WVYLKVLSWSWL 361
            K+                THFF  + L        L      + +L W   K  +W WL
Sbjct: 253 LKYNSQIENLSQHGLHSRLTHFFTNMPL--------LCGPLIFVPKL-WDVRKPATWLWL 303

Query: 362 TVLCHFWLGFFLQQIXXXXXXXXXXXXXXXXCPERKVWRGRIFLL 496
             L  F L  F  Q                 C  R  W   +F++
Sbjct: 304 --LPVFILSLFPHQEPRFLLPAASIFIVNSGCLVRSYWIKFLFVM 346


>SPCC1902.01 |gaf1|SPCC417.01c|transcription factor Gaf1
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 855

 Score = 27.9 bits (59), Expect = 1.7
 Identities = 24/62 (38%), Positives = 31/62 (50%), Gaps = 2/62 (3%)
 Frame = -1

Query: 470 PSFLGIAPTRKAAA--TSLKASSGFAVGRTPAKSGRAQSANSMTTPSSTPIIGAISRRWR 297
           P F  + P RK +A  T+L  SS       P  + RA SANS+T+P   P   A S + R
Sbjct: 516 PQFSAV-PHRKVSAQDTNLMGSSPGMYNHMPYLN-RATSANSITSPGVLPEGMAASLKKR 573

Query: 296 KT 291
            T
Sbjct: 574 TT 575


>SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1036

 Score = 27.9 bits (59), Expect = 1.7
 Identities = 23/89 (25%), Positives = 37/89 (41%), Gaps = 5/89 (5%)
 Frame = -1

Query: 575 PTTRTFTSLRATLSKAWPCSLKMRTLAARRSFLSIPSFLGIAPTRKAA-----ATSLKAS 411
           P+  T + L  +   + P S    T A   S   + S      T  ++     +T   ++
Sbjct: 521 PSYNTSSVLPTSSVSSTPLSSANSTTATSASSTPLTSVNSTTATSASSTPFGNSTITSSA 580

Query: 410 SGFAVGRTPAKSGRAQSANSMTTPSSTPI 324
           SG     T   SG    + S+TTP+STP+
Sbjct: 581 SGSTGEFTNTNSGNGDVSGSVTTPTSTPL 609


>SPAC105.03c |||transcription factor |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 708

 Score = 26.6 bits (56), Expect = 4.0
 Identities = 11/30 (36%), Positives = 17/30 (56%)
 Frame = -3

Query: 690 SKLGLSPVLIETSHGSEIFFWNRWSILRTY 601
           +K  LSP+L+   + S I +W+    LR Y
Sbjct: 337 AKHKLSPILLTAMYASSIIYWDADKELRNY 366


>SPAC13A11.06 ||SPAC3H8.01|pyruvate decarboxylase
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 571

 Score = 26.2 bits (55), Expect = 5.3
 Identities = 8/22 (36%), Positives = 15/22 (68%)
 Frame = -1

Query: 629 GIDGAYLEHIRAFVLAGLPTTR 564
           G+ G+Y EH+    + G+P+T+
Sbjct: 87  GVAGSYAEHVPVVHIVGMPSTK 108


>SPBC1709.01 |chs2|SPBC1734.17|chitin synthase homolog
           Chs2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 926

 Score = 25.8 bits (54), Expect = 7.0
 Identities = 14/40 (35%), Positives = 22/40 (55%), Gaps = 1/40 (2%)
 Frame = -3

Query: 765 QGTGLMNGSPDD-YSLNIFNRYSNFSSKLGLSPVLIETSH 649
           Q T  +N + DD Y  +  N Y +F+S++  SPV    +H
Sbjct: 122 QPTHTVNSNSDDGYENSSKNEYLDFNSEISASPVNEPMTH 161


>SPAC1F8.07c |||pyruvate decarboxylase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 594

 Score = 25.8 bits (54), Expect = 7.0
 Identities = 10/26 (38%), Positives = 16/26 (61%)
 Frame = -1

Query: 629 GIDGAYLEHIRAFVLAGLPTTRTFTS 552
           GI GAY E++   +++G P T   +S
Sbjct: 89  GIGGAYAENLPVILVSGSPNTNDLSS 114


>SPAC31A2.05c |mis4||cohesin loading factor Mis4|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1583

 Score = 25.4 bits (53), Expect = 9.3
 Identities = 14/41 (34%), Positives = 20/41 (48%)
 Frame = -1

Query: 377 SGRAQSANSMTTPSSTPIIGAISRRWRKTGC*GPKTAPDAI 255
           + R+ S N   TPSS+P   + SR +       PK + D I
Sbjct: 147 TNRSSSDNGFLTPSSSPRSPSCSRVFNAVQLCSPKKSKDDI 187


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,232,430
Number of Sequences: 5004
Number of extensions: 65875
Number of successful extensions: 207
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 201
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 207
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 381366860
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)