Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fmgV16l16f
         (690 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC167.02 |ptb1||geranylgeranyltransferase II beta subunit |Sch...   142   3e-35
SPAC17G6.04c |cpp1||protein farnesyltransferase beta subunit Cpp...    68   1e-12
SPAC2E1P5.04c |cwg2|orb7|geranylgeranyltransferase I beta subuni...    47   2e-06
SPAC13G7.01c |erg7|SPAC4G9.21c|lanosterol synthase Erg7 |Schizos...    33   0.029
SPAC23C4.10 |sec2||guanyl-nucleotide exchange factor Sec2 |Schiz...    30   0.27 
SPBC3B9.04 |||mitochondrial methyltransferase |Schizosaccharomyc...    28   1.5  
SPBC577.06c |||phosphatidylinositol kinase |Schizosaccharomyces ...    28   1.5  
SPAPB1E7.02c |mcl1|slr3|DNA polymerase alpha accessory factor Mc...    27   1.9  
SPCC320.12 ||SPCC330.17c|mitochondrial inner membrane peptidase ...    27   3.4  

>SPAC167.02 |ptb1||geranylgeranyltransferase II beta subunit
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 311

 Score =  142 bits (345), Expect = 3e-35
 Identities = 58/127 (45%), Positives = 85/127 (66%)
 Frame = +2

Query: 308 IKVLAMYDRLDAVDIEGIVRYIVSLQQEDGSFYGDKWGEIDTRFSFCAVMCLSLLQRLDA 487
           ++VLAM D L  VD + +  YI+ LQ EDGS  GD+WGEID RF +  + CL++L +LD 
Sbjct: 88  VQVLAMLDSLHVVDKDKVASYIIGLQNEDGSMKGDRWGEIDARFLYSGINCLAILGKLDY 147

Query: 488 IDINKTVEFVLSCMNFDGGFGSKPGSESHAGLIYCCVGTLSICKRMDALHADELAWWLCE 667
           ++ N  V++++ C NFDGGFG  PG+ESH  +++ CV  L I  ++D +  + L WW+ E
Sbjct: 148 LNKNTAVDWLMKCYNFDGGFGLCPGAESHGAMVFTCVAALKILNKLDLIDEELLGWWISE 207

Query: 668 RQLPSGG 688
           RQ+  GG
Sbjct: 208 RQVKGGG 214



 Score = 48.0 bits (109), Expect = 1e-06
 Identities = 30/117 (25%), Positives = 51/117 (43%)
 Frame = +2

Query: 338 DAVDIEGIVRYIVSLQQEDGSFYGDKWGEIDTRFSFCAVMCLSLLQRLDAIDINKTVEFV 517
           D +D E IV +++S   E G F      +     +  AV  L++L  L  +D +K   ++
Sbjct: 50  DQIDKERIVSFLLSCLTESGGFACYPGHDDHITNTVYAVQVLAMLDSLHVVDKDKVASYI 109

Query: 518 LSCMNFDGGFGSKPGSESHAGLIYCCVGTLSICKRMDALHADELAWWLCERQLPSGG 688
           +   N DG        E  A  +Y  +  L+I  ++D L+ +    WL +     GG
Sbjct: 110 IGLQNEDGSMKGDRWGEIDARFLYSGINCLAILGKLDYLNKNTAVDWLMKCYNFDGG 166



 Score = 43.6 bits (98), Expect = 3e-05
 Identities = 24/78 (30%), Positives = 39/78 (50%), Gaps = 2/78 (2%)
 Frame = +2

Query: 458 CLS--LLQRLDAIDINKTVEFVLSCMNFDGGFGSKPGSESHAGLIYCCVGTLSICKRMDA 631
           C+S  LL++ D ID  + V F+LSC+   GGF   PG + H       V  L++   +  
Sbjct: 40  CMSFWLLKKKDQIDKERIVSFLLSCLTESGGFACYPGHDDHITNTVYAVQVLAMLDSLHV 99

Query: 632 LHADELAWWLCERQLPSG 685
           +  D++A ++   Q   G
Sbjct: 100 VDKDKVASYIIGLQNEDG 117



 Score = 37.9 bits (84), Expect = 0.001
 Identities = 23/86 (26%), Positives = 45/86 (52%), Gaps = 1/86 (1%)
 Frame = +2

Query: 302 TIIKVLAMYDRLDAVDIEGIVRYIVSLQQEDGSFYGDKWGEIDTRFSFCAVMCLSLLQRL 481
           T +  L + ++LD +D E +  +I   Q + G   G      D+ + +  +  L+++ +L
Sbjct: 182 TCVAALKILNKLDLIDEELLGWWISERQVKGGGLNGRPEKLPDSCYGWWDLSPLAIIGKL 241

Query: 482 DAIDINKTVEFVLSCMNFD-GGFGSK 556
           D ID N+ ++F+L   + D GGF  +
Sbjct: 242 DWIDRNQLIDFLLGTQDADSGGFADR 267



 Score = 37.1 bits (82), Expect = 0.002
 Identities = 16/43 (37%), Positives = 24/43 (55%)
 Frame = +3

Query: 171 LLLQKHSDYLASYGLNKDDYEYCMTEYLRMSGIYWSLTAMELM 299
           LL  KH  YL   G   D+ ++ + E+L +S IYWS  +  L+
Sbjct: 4   LLRDKHISYLHDIGNRTDELDFWLKEHLHVSAIYWSCMSFWLL 46


>SPAC17G6.04c |cpp1||protein farnesyltransferase beta subunit
           Cpp1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 382

 Score = 68.1 bits (159), Expect = 1e-12
 Identities = 38/135 (28%), Positives = 73/135 (54%), Gaps = 5/135 (3%)
 Frame = +2

Query: 293 THGTIIKVLAMYDRLDA---VDIEGIVRYIVSLQQEDGSFYGDKWGEIDTRFSFCAVMCL 463
           T+ +I+ +  + D  DA   ++ + +  ++ SL+  DGSF  +  GE D R  + AV C+
Sbjct: 108 TYASILSI-CLCDSTDAYSLIERDRLYDWLFSLKNPDGSFRVNNEGESDARSVYAAV-CV 165

Query: 464 SLLQRLDAID--INKTVEFVLSCMNFDGGFGSKPGSESHAGLIYCCVGTLSICKRMDALH 637
           S L  +   D     T++++  C  ++GG    P +E+H G  +C +  +++   +D L+
Sbjct: 166 SSLVGISMDDPLFEGTLQWLCKCQTYEGGLSGVPYAEAHGGYTFCALAAIALLGGLDNLN 225

Query: 638 ADELAWWLCERQLPS 682
             +L+ WL +RQ P+
Sbjct: 226 EIKLSTWLVQRQDPA 240



 Score = 41.9 bits (94), Expect = 8e-05
 Identities = 34/129 (26%), Positives = 56/129 (43%), Gaps = 5/129 (3%)
 Frame = +2

Query: 317 LAMYDRLDAVDIEGIVRYIVSLQQEDGSFYGDKWGEIDTRFSFCAVMCLSLLQRLDA--- 487
           LA+  +LD+   E  +  +  L+   G F G    +     ++ +++ + L    DA   
Sbjct: 67  LAILGKLDSSVCERAISSVRQLKGPSGGFCGGNGQDEHLLSTYASILSICLCDSTDAYSL 126

Query: 488 IDINKTVEFVLSCMNFDGGFGSKPGSESHAGLIYC--CVGTLSICKRMDALHADELAWWL 661
           I+ ++  +++ S  N DG F      ES A  +Y   CV +L      D L    L  WL
Sbjct: 127 IERDRLYDWLFSLKNPDGSFRVNNEGESDARSVYAAVCVSSLVGISMDDPLFEGTLQ-WL 185

Query: 662 CERQLPSGG 688
           C+ Q   GG
Sbjct: 186 CKCQTYEGG 194


>SPAC2E1P5.04c |cwg2|orb7|geranylgeranyltransferase I beta subunit
           Cwg2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 355

 Score = 47.2 bits (107), Expect = 2e-06
 Identities = 40/153 (26%), Positives = 65/153 (42%), Gaps = 20/153 (13%)
 Frame = +2

Query: 290 GTHGTIIKVLAMYDRLDAVDIEGIVRYIVSLQQEDGSFYG---DKWGEIDTRFSFCAVMC 460
           GT  +I  +L + D L  +D + I  ++   +   G F         + D R  + A   
Sbjct: 99  GTVFSICCLLFLGDNLSRIDRDLIKNFVELCKTSQGHFRSIAVPSCSDQDMRQLYMATTI 158

Query: 461 LSLLQRLDAIDINKTVEFVLSCMNFDGGFGSKPGSESHAGLIYCCVGTLSICKRM----- 625
            SLL      D   +++++ SC  ++GGF   P  E+HAG  +C + + S+  +M     
Sbjct: 159 ASLLD-FSLSDPLCSIQYIKSCQRYEGGFSLLPYGEAHAGATFCALASWSLILKMIPNSS 217

Query: 626 -----------DAL-HADELAWWLCERQLPSGG 688
                      D +   + L  WL  RQL SGG
Sbjct: 218 LNTSNQSYNLMDCVPKVERLIRWLASRQLSSGG 250


>SPAC13G7.01c |erg7|SPAC4G9.21c|lanosterol synthase Erg7
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 721

 Score = 33.5 bits (73), Expect = 0.029
 Identities = 27/87 (31%), Positives = 41/87 (47%), Gaps = 7/87 (8%)
 Frame = +2

Query: 308 IKVLAMYD---RLDAVD--IEGIVRYIVSLQQEDGSFYGDKWGEIDTRFSFCAVMCLSLL 472
           ++    YD   R D ++  IE  + Y+V +Q+ DGS+YG  W    T  +  A   L+  
Sbjct: 536 LRAFTKYDPGYRRDEIENTIENALEYVVKMQRPDGSWYG-SWAICFTYAAMFATGSLASA 594

Query: 473 QRL--DAIDINKTVEFVLSCMNFDGGF 547
            R   +     K  EF+LS    DGG+
Sbjct: 595 GRYYENCPVQKKACEFLLSKQRPDGGW 621


>SPAC23C4.10 |sec2||guanyl-nucleotide exchange factor Sec2
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 527

 Score = 30.3 bits (65), Expect = 0.27
 Identities = 13/31 (41%), Positives = 16/31 (51%)
 Frame = +2

Query: 545 FGSKPGSESHAGLIYCCVGTLSICKRMDALH 637
           F S+P S SHA   YC     S+C  +  LH
Sbjct: 458 FRSRPDSTSHASCTYCVARLRSVCNFVAFLH 488


>SPBC3B9.04 |||mitochondrial methyltransferase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 248

 Score = 27.9 bits (59), Expect = 1.5
 Identities = 17/47 (36%), Positives = 25/47 (53%)
 Frame = +3

Query: 135 KDVVLKEDRPKTLLLQKHSDYLASYGLNKDDYEYCMTEYLRMSGIYW 275
           KDV+  E R K+ LL  +S+ ++ Y    D Y   +T     SGIY+
Sbjct: 29  KDVLNYESRQKSDLLIPNSNSISIYNQIADKYSRKITREEIFSGIYF 75


>SPBC577.06c |||phosphatidylinositol kinase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 1877

 Score = 27.9 bits (59), Expect = 1.5
 Identities = 12/30 (40%), Positives = 16/30 (53%)
 Frame = +3

Query: 186 HSDYLASYGLNKDDYEYCMTEYLRMSGIYW 275
           HS+Y A +GL+   Y   M  Y+  SG  W
Sbjct: 229 HSNYYAGFGLSYYLYLSSMKAYINSSGCLW 258


>SPAPB1E7.02c |mcl1|slr3|DNA polymerase alpha accessory factor
           Mcl1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 815

 Score = 27.5 bits (58), Expect = 1.9
 Identities = 9/27 (33%), Positives = 18/27 (66%)
 Frame = +2

Query: 509 EFVLSCMNFDGGFGSKPGSESHAGLIY 589
           +F ++C++ +G   + P +ES  G+IY
Sbjct: 471 KFEMACLDHEGALYASPATESSPGVIY 497


>SPCC320.12 ||SPCC330.17c|mitochondrial inner membrane peptidase
           Atp23|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 185

 Score = 26.6 bits (56), Expect = 3.4
 Identities = 11/32 (34%), Positives = 15/32 (46%)
 Frame = -2

Query: 581 DQHGFPIQVWNRIHHQSSCN*AQTPQSC*CRW 486
           D H F +  WN + HQ+      +  S  CRW
Sbjct: 93  DDHRFEVD-WNNLRHQACSEIRASSMSGECRW 123


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,898,591
Number of Sequences: 5004
Number of extensions: 59652
Number of successful extensions: 169
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 156
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 168
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 319939482
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)