Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fmgV16e21f
(754 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC1683.07 |mal1||alpha-glucosidase Mal1 |Schizosaccharomyces p... 233 2e-62
SPAC23D3.14c |aah2||alpha-amylase homolog Aah2|Schizosaccharomyc... 66 5e-12
SPCC63.02c |aah3||alpha-amylase homolog Aah3|Schizosaccharomyces... 58 1e-09
SPCC11E10.09c ||SPCC188.01c|alpha-amylase homolog |Schizosacchar... 58 1e-09
SPAC27E2.01 |||alpha-amylase homolog |Schizosaccharomyces pombe|... 50 4e-07
SPCC757.12 |||alpha-amylase homolog |Schizosaccharomyces pombe|c... 50 4e-07
SPAC25H1.09 |mde5|meu30, SPAC4A8.01|alpha-amylase homolog Mde5|S... 45 1e-05
SPBC16A3.13 |meu7|aah4|alpha-amylase homolog Aah4|Schizosaccharo... 42 9e-05
SPBC16D10.05 |mok13||alpha-1,3-glucan synthase Mok13|Schizosacch... 41 2e-04
SPAC1527.01 |mok11|SPAC23D3.15|alpha-1,3-glucan synthase Mok11|S... 39 7e-04
SPBC32H8.13c |mok12||alpha-1,3-glucan synthase Mok12|Schizosacch... 36 0.005
SPCC1281.01 |ags1|mok1, SPCC338.01c, SPCC17A7.01|alpha-1,4-gluca... 35 0.014
SPAC458.06 |||phosphoinositide binding protein|Schizosaccharomyc... 30 0.31
SPCC1795.08c |||histone acetyltransferase complex subunit |Schiz... 27 2.2
SPBC29A10.10c |||tRNA-splicing endonuclease positive effector |S... 26 5.0
SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||M... 26 5.0
SPAC19A8.11c |||recombination protein Irc6 |Schizosaccharomyces ... 26 6.6
SPBC428.03c |pho4||thiamine-repressible acid phosphatase Pho4|Sc... 25 8.8
SPBC24C6.03 |||proline-tRNA ligase |Schizosaccharomyces pombe|ch... 25 8.8
>SPBC1683.07 |mal1||alpha-glucosidase Mal1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 579
Score = 233 bits (571), Expect = 2e-62
Identities = 101/207 (48%), Positives = 146/207 (70%), Gaps = 2/207 (0%)
Frame = +2
Query: 137 DWWETSILYQIYPRSFADSDGDGIGDLNGITSKLEYIKELGVGAVWLSPIFKSPMVDFGY 316
+WW + +YQIYP SF DS+GDG GDL GI SK++Y+K L V ++WL PI+ SP+ D GY
Sbjct: 12 NWWRETSVYQIYPASFKDSNGDGFGDLEGIISKVDYLKALNVESIWLCPIYPSPLKDMGY 71
Query: 317 DIANFYEIHHEYGTMEDFEALLKKANELDIKVVLDLVPNHTSNESVWFQEALNG--NEKY 490
D++++ +I YGT+ED + L+K +E D+K+V+DLV NHTS++ WF+E+ + N K
Sbjct: 72 DVSDYKQIDSRYGTLEDLDRLMKALHERDMKLVMDLVLNHTSDQHEWFKESRSSKTNPKR 131
Query: 491 YNYFVWEDGIIDENGNRQPPNNWLSHFRGSAWEYKEEVGKYYLHQFAVGQPDLNYRNQDV 670
YF W+ +E G R PPNNW S+F SAWE+ E +YYLH ++VGQPDLN+ V
Sbjct: 132 DWYF-WKPARYNEKGERLPPNNWRSYFDTSAWEWDEATQEYYLHLWSVGQPDLNWETPKV 190
Query: 671 VDEMKNIIRFWLGKGIAGFRVDAVNCL 751
+ + +I+RFWL +G+ GFR+DA+N +
Sbjct: 191 REAVHDILRFWLDRGVDGFRLDAINMI 217
>SPAC23D3.14c |aah2||alpha-amylase homolog Aah2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 581
Score = 66.1 bits (154), Expect = 5e-12
Identities = 40/130 (30%), Positives = 71/130 (54%), Gaps = 12/130 (9%)
Frame = +2
Query: 209 GDLNGITSKLEYIKELGVGAVWLSPIFKSPM---VD----FGYDIANFYEIHHEYGTMED 367
G NGI + L+YI+ +G A+W+SPIF++ +D GY N YE++H +GT E+
Sbjct: 66 GTWNGIRNHLDYIQGMGFDAIWISPIFENVEGNDIDGSSYHGYWTTNLYELNHHFGTKEE 125
Query: 368 FEALLKKANELDIKVVLDLVPNHTS----NESVWFQEALNGNE-KYYNYFVWEDGIIDEN 532
F L+++ ++ DI ++LD+ N + E + F++ + N+ +++ W D + N
Sbjct: 126 FMELIQELHKRDIWILLDVAINSMAINGPLEQMSFEKVIPFNDASFFHPHCWVD--YESN 183
Query: 533 GNRQPPNNWL 562
N WL
Sbjct: 184 DIESVQNCWL 193
>SPCC63.02c |aah3||alpha-amylase homolog Aah3|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 564
Score = 58.0 bits (134), Expect = 1e-09
Identities = 41/136 (30%), Positives = 66/136 (48%), Gaps = 20/136 (14%)
Frame = +2
Query: 86 LLFVACSGIIIKNGEVQDWWETSILYQIYPRSFADSDGDGI------------GDLNGIT 229
LLF++ + I + N W I+YQI FA DG G GI
Sbjct: 8 LLFLSSALIHVANAGSNAEWRKRIIYQILTDRFAVDDGSTDNPCDPDANQYCGGTWKGIE 67
Query: 230 SKLEYIKELGVGAVWLSPIFKSPMVDF--------GYDIANFYEIHHEYGTMEDFEALLK 385
+KL+YI+++G A+W+SPI K+ D GY ++ ++ +GT +D +L+
Sbjct: 68 NKLDYIEDMGFNAIWISPIDKNIEGDIDGAGYAYHGYWNTDYESLNEHFGTEDDLVSLIT 127
Query: 386 KANELDIKVVLDLVPN 433
A++ I V+LD + N
Sbjct: 128 AAHKAGIWVMLDSIVN 143
>SPCC11E10.09c ||SPCC188.01c|alpha-amylase homolog
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 478
Score = 58.0 bits (134), Expect = 1e-09
Identities = 46/155 (29%), Positives = 73/155 (47%), Gaps = 22/155 (14%)
Frame = +2
Query: 101 CSGIIIKNGEVQDWWETSILYQIYPRSFA-DSDG---DGIGDL------NGITSKLEYIK 250
CS + K W ++YQ+ FA D D G+L GIT L+YIK
Sbjct: 5 CSILPKKEPSYLKLWRKQVIYQVLTDRFALDEDNFYAKASGNLYLGGTWKGITRNLDYIK 64
Query: 251 ELGVGAVWLSPIFK--SPMVD-----FGYDIANFYEIHHEYGTMEDFEALLKKANELDIK 409
LG A+W+SPI K S D GY + +++ +GT ED + L+ +E ++
Sbjct: 65 SLGCTAIWISPIVKNISETTDCGQAYHGYWAQDMTQLNENFGTEEDLKELVNAIHEKNML 124
Query: 410 VVLDLVPN---HTSNESVWF--QEALNGNEKYYNY 499
++D+V N H ++ V F + N + Y+N+
Sbjct: 125 CMVDIVVNHMGHAGSKPVNFLLYQPFNSGKYYHNW 159
>SPAC27E2.01 |||alpha-amylase homolog |Schizosaccharomyces pombe|chr
1|||Manual
Length = 491
Score = 50.0 bits (114), Expect = 4e-07
Identities = 36/121 (29%), Positives = 59/121 (48%), Gaps = 17/121 (14%)
Frame = +2
Query: 134 QDWWETSILYQIYPRSFADSD------GDGI----GDLNGITSKLEYIKELGVGAVWLSP 283
+D W +YQI FA D G G GI KL+YI+ LG AVW+SP
Sbjct: 21 KDIWRRQCIYQILTDRFALDDHCTTAPSTGRMYLGGTWRGIIQKLDYIQSLGCTAVWISP 80
Query: 284 IFKSPMVDFGYDIA-------NFYEIHHEYGTMEDFEALLKKANELDIKVVLDLVPNHTS 442
I K+ GY A + +++ +GT +D L+ + ++ ++ ++D+V NH +
Sbjct: 81 IVKNIEGVTGYGEAYHGYWAEDLTQLNPHFGTKQDLTELVDQLHKRNMLCMIDIVVNHMA 140
Query: 443 N 445
+
Sbjct: 141 H 141
>SPCC757.12 |||alpha-amylase homolog |Schizosaccharomyces pombe|chr
3|||Manual
Length = 625
Score = 50.0 bits (114), Expect = 4e-07
Identities = 45/187 (24%), Positives = 84/187 (44%), Gaps = 24/187 (12%)
Frame = +2
Query: 71 VCLLSLLFVACSGIIIKNGEVQDWWETSILYQIYPRSFA-DSDG-------DGI---GDL 217
+ L SL A G+ + + W I+YQ+ FA DSD D G
Sbjct: 6 IALFSLF--ALFGLPTSLAKSSEEWRDRIIYQVITDRFAVDSDNTPDCSFDDSSYCGGTW 63
Query: 218 NGITSKLEYIKELGVGAVWLSPIFKSPMVDFGYD--------IANFYEIHHEYGTMEDFE 373
+GI SKL+YI+ +G A+W+SP+ K+ +G D +F +++ +G+ +D
Sbjct: 64 SGIRSKLDYIQGMGFNAIWISPVEKNLEGSYGSDGEAYHGYWNTDFTQLNEHFGSEDDLI 123
Query: 374 ALLKKANELDIKVVLDLVPNHTS----NESVWFQEALNGNE-KYYNYFVWEDGIIDENGN 538
L+ + D+ ++ D + N + +++ + + N+ Y++ + W D N N
Sbjct: 124 DLITDMHNRDMWIMFDALANSMAIPGPTDNISYSNLVPFNDSSYFHPYCWID--YGSNNN 181
Query: 539 RQPPNNW 559
+ W
Sbjct: 182 TDIEDCW 188
>SPAC25H1.09 |mde5|meu30, SPAC4A8.01|alpha-amylase homolog
Mde5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 513
Score = 44.8 bits (101), Expect = 1e-05
Identities = 25/83 (30%), Positives = 43/83 (51%), Gaps = 7/83 (8%)
Frame = +2
Query: 209 GDLNGITSKLEYIKELGVGAVWLSPIFKS-------PMVDFGYDIANFYEIHHEYGTMED 367
G+ GI KL+YI+ +G A+W+SPI K+ GY + Y ++ +GT +D
Sbjct: 62 GNWRGIIDKLDYIQGMGFTAIWISPIIKNIEGRTKYGEAYHGYWPQDLYTLNPHFGTEQD 121
Query: 368 FEALLKKANELDIKVVLDLVPNH 436
L ++ + +++D V NH
Sbjct: 122 LIDLADALHDRGMYLMVDTVVNH 144
>SPBC16A3.13 |meu7|aah4|alpha-amylase homolog
Aah4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 774
Score = 41.9 bits (94), Expect = 9e-05
Identities = 24/87 (27%), Positives = 45/87 (51%), Gaps = 7/87 (8%)
Frame = +2
Query: 209 GDLNGITSKLEYIKELGVGAVWLSPI---FKSPMVD----FGYDIANFYEIHHEYGTMED 367
G+ GI L+YI++LG A+ +SP+ + P G+ N+Y ++ +G ED
Sbjct: 62 GNWQGIVDHLDYIRDLGFTAISISPVVEQLEGPEYHGEAYHGHRPKNYYRLNPHFGNEED 121
Query: 368 FEALLKKANELDIKVVLDLVPNHTSNE 448
+ L + + +++D+ NHT +E
Sbjct: 122 LKELSDALHGKGMYLMVDVAINHTISE 148
>SPBC16D10.05 |mok13||alpha-1,3-glucan synthase
Mok13|Schizosaccharomyces pombe|chr 2|||Manual
Length = 2358
Score = 40.7 bits (91), Expect = 2e-04
Identities = 34/147 (23%), Positives = 66/147 (44%), Gaps = 8/147 (5%)
Frame = +2
Query: 161 YQIYPRSFADSDGDGIGDLNGITSKLEYIKELGVGAVWL--SPIFKSPMVDFGYDIANFY 334
Y IY F + GD+ G+ L+Y++ +G+ +++ +P P Y ++
Sbjct: 90 YDIYETGFRNG-----GDIIGLKDSLDYLEIMGIKVIYIAGTPFLNQPWGADQYSPLDYT 144
Query: 335 EIHHEYGTMEDFEALLKKANELDIKVVLDLVPNHTSNESVWFQEALNGNEKYYNY---FV 505
+ H GT+ + +++ + +VLDL + T + + F++ LN + + V
Sbjct: 145 ILDHHSGTIAQWRDTIEEIHRRGFYLVLDLTIS-TLGDLIGFRKYLNSTTPFSLFEHEAV 203
Query: 506 WEDGII--DEN-GNRQPPNNWLSHFRG 577
W+ +I D N N+ P L F G
Sbjct: 204 WKSNVIYPDWNFTNKYDPKCELPRFWG 230
>SPAC1527.01 |mok11|SPAC23D3.15|alpha-1,3-glucan synthase
Mok11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2397
Score = 39.1 bits (87), Expect = 7e-04
Identities = 25/98 (25%), Positives = 46/98 (46%), Gaps = 2/98 (2%)
Frame = +2
Query: 137 DWWETSILYQIYPRSFADSDGDGIGDLNGITSKLEYIKELGVGAVWL--SPIFKSPMVDF 310
D+ T Y IY F + GD+ G+ L+Y++ +GV AV+ +P P
Sbjct: 83 DFSGTRFEYDIYETEFRNG-----GDIIGVRQSLDYLQGMGVKAVYFAGTPFVNMPWGAD 137
Query: 311 GYDIANFYEIHHEYGTMEDFEALLKKANELDIKVVLDL 424
Y +F + GT+ D+ +++ + + V++DL
Sbjct: 138 QYSPLDFTLLDPHLGTINDWRGTIEEMHSKGMYVIVDL 175
>SPBC32H8.13c |mok12||alpha-1,3-glucan synthase
Mok12|Schizosaccharomyces pombe|chr 2|||Manual
Length = 2352
Score = 36.3 bits (80), Expect = 0.005
Identities = 22/93 (23%), Positives = 45/93 (48%), Gaps = 4/93 (4%)
Frame = +2
Query: 209 GDLNGITS--KLEYIKELGVGAVWLS--PIFKSPMVDFGYDIANFYEIHHEYGTMEDFEA 376
GD+ G+ + L+Y++ +G+ AV+++ P P GY +F + GT+ +
Sbjct: 103 GDIVGLVTPRSLDYLESMGIKAVYIAGTPFQNLPWYPDGYSPLDFTLLDKHTGTLNQWHE 162
Query: 377 LLKKANELDIKVVLDLVPNHTSNESVWFQEALN 475
+ K +E VV+D + S S + +++
Sbjct: 163 AIMKLHERGFYVVVDFTISTLSELSYFVNSSMS 195
>SPCC1281.01 |ags1|mok1, SPCC338.01c, SPCC17A7.01|alpha-1,4-glucan
synthase Ags1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 2410
Score = 34.7 bits (76), Expect = 0.014
Identities = 17/74 (22%), Positives = 38/74 (51%), Gaps = 2/74 (2%)
Frame = +2
Query: 209 GDLNGITSKLEYIKELGVGAVWL--SPIFKSPMVDFGYDIANFYEIHHEYGTMEDFEALL 382
GD+ G+ L+Y++ LG +++ +P P Y ++ + H GT++ + + +
Sbjct: 106 GDIVGLELSLDYLEGLGTQGIYIAGTPFVNMPWGADQYSPLDYTILDHHLGTIDQWRSTI 165
Query: 383 KKANELDIKVVLDL 424
+E + +V+DL
Sbjct: 166 TAMHERGMYLVVDL 179
Score = 26.6 bits (56), Expect = 3.8
Identities = 10/28 (35%), Positives = 16/28 (57%)
Frame = +2
Query: 551 NNWLSHFRGSAWEYKEEVGKYYLHQFAV 634
N WLS G +W Y ++G +HQ+ +
Sbjct: 2209 NFWLSSLNGRSWSYLWDIGN--IHQWQI 2234
>SPAC458.06 |||phosphoinositide binding protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 364
Score = 30.3 bits (65), Expect = 0.31
Identities = 13/32 (40%), Positives = 18/32 (56%)
Frame = +1
Query: 433 SYIKRKCLVPRSSQWQRKILQLFRLGRRNHRR 528
S K PR + W+RK+L+L G+R H R
Sbjct: 269 SEFKSSTTEPRQTHWKRKLLKLIDSGKRAHWR 300
>SPCC1795.08c |||histone acetyltransferase complex subunit
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 985
Score = 27.5 bits (58), Expect = 2.2
Identities = 16/37 (43%), Positives = 20/37 (54%), Gaps = 1/37 (2%)
Frame = -2
Query: 282 GESQTAPTPN-SLIYSNFEVIPFKSPIPSPSLSAKDL 175
G S+ P SL S E+I KSPIPSP K++
Sbjct: 118 GVSEETPLKETSLELSEEEIITSKSPIPSPETIHKNI 154
>SPBC29A10.10c |||tRNA-splicing endonuclease positive effector
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1944
Score = 26.2 bits (55), Expect = 5.0
Identities = 11/48 (22%), Positives = 24/48 (50%)
Frame = -1
Query: 652 VQVWLSNSELM*VIFSDFFFVFPSAASKVTQPIIRWLPISIFVYDSVF 509
VQ+ S +++ V+FS++F +FP + + + +D +F
Sbjct: 391 VQLLQSLADIFSVLFSEYFMIFPHSDQSLQNKVFELHSTLAIKFDELF 438
>SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr
1|||Manual
Length = 663
Score = 26.2 bits (55), Expect = 5.0
Identities = 12/33 (36%), Positives = 19/33 (57%)
Frame = +2
Query: 275 LSPIFKSPMVDFGYDIANFYEIHHEYGTMEDFE 373
+ P K ++D D+A + EIH E + E+FE
Sbjct: 591 VDPEDKQAVLDAVEDVAEWLEIHGEDASKEEFE 623
>SPAC19A8.11c |||recombination protein Irc6 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 246
Score = 25.8 bits (54), Expect = 6.6
Identities = 15/46 (32%), Positives = 23/46 (50%)
Frame = +2
Query: 560 LSHFRGSAWEYKEEVGKYYLHQFAVGQPDLNYRNQDVVDEMKNIIR 697
LS S E K++ G +YL + G PD+ + D + + N IR
Sbjct: 168 LSQVDKSFSEEKDDAGDFYLPERFKGSPDMQSTDLDNLMKEMNQIR 213
>SPBC428.03c |pho4||thiamine-repressible acid phosphatase
Pho4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 463
Score = 25.4 bits (53), Expect = 8.8
Identities = 12/33 (36%), Positives = 16/33 (48%)
Frame = +2
Query: 473 NGNEKYYNYFVWEDGIIDENGNRQPPNNWLSHF 571
N YY+ V+ED IDEN +W + F
Sbjct: 202 NSLAMYYSCPVYEDNNIDENTTEAAHTSWRNVF 234
>SPBC24C6.03 |||proline-tRNA ligase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 425
Score = 25.4 bits (53), Expect = 8.8
Identities = 11/31 (35%), Positives = 19/31 (61%)
Frame = +1
Query: 433 SYIKRKCLVPRSSQWQRKILQLFRLGRRNHR 525
+++ K + +S +WQ+ +LFRL RN R
Sbjct: 77 AHLSSKEIWEKSGRWQKTGSELFRLHDRNDR 107
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,426,319
Number of Sequences: 5004
Number of extensions: 79523
Number of successful extensions: 255
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 234
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 247
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 359287726
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -