BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fmgV16d15f
(760 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC16E8.10c |||mitochondrial ribosomal protein subunit S7|Schiz... 52 7e-08
SPBC11C11.03 |ndc80|ndc10, tid3|spindle pole body protein Ndc80|... 29 0.72
SPBC13E7.05 |gpi14||pig-M|Schizosaccharomyces pombe|chr 2|||Manual 28 1.3
SPAC144.05 |||ATP-dependent DNA helicase|Schizosaccharomyces pom... 28 1.7
SPAC4A8.11c |fas2|lsd1|fatty acid synthase alpha subunit Lsd1 |S... 27 2.2
SPBP8B7.09c |||karyopherin|Schizosaccharomyces pombe|chr 2|||Manual 27 2.2
SPBC1709.01 |chs2|SPBC1734.17|chitin synthase homolog Chs2|Schiz... 27 2.2
SPAC1805.06c |hem2||porphobilinogen synthase Hem2 |Schizosacchar... 27 2.9
SPAC32A11.04c |tif212|tif22, SPAC6B12.17c|translation initiation... 27 2.9
SPBC26H8.08c |grn1||GTPase Grn1 |Schizosaccharomyces pombe|chr 2... 27 2.9
SPAC1006.04c |mcp3|mug7|sequence orphan|Schizosaccharomyces pomb... 26 5.1
SPCC306.09c |cap1|cap|adenylyl cyclase-associated protein Cap1|S... 26 6.7
SPAC4G8.03c |||RNA-binding protein|Schizosaccharomyces pombe|chr... 26 6.7
SPAC22G7.06c |ura1||carbamoyl-phosphate synthase |Schizosaccharo... 25 8.9
SPAC631.02 |||bromodomain protein|Schizosaccharomyces pombe|chr ... 25 8.9
>SPAC16E8.10c |||mitochondrial ribosomal protein subunit
S7|Schizosaccharomyces pombe|chr 1|||Manual
Length = 259
Score = 52.4 bits (120), Expect = 7e-08
Identities = 35/143 (24%), Positives = 64/143 (44%)
Frame = +2
Query: 329 DPLVNKVINHVMEKGNKRLARQLVEKAFENIKRKQIERYHLASTPEEKAKIELNPKDILY 508
D V ++N +M G K A ++V A I+++ E NP D+L
Sbjct: 123 DSTVQHLVNLIMRDGKKAKAEKIVATALSIIQKETGE----------------NPIDVLK 166
Query: 509 KAVENCKPLLQLQPIKRGGITYQVPGPITEKRSLFLAIKWLLETTDEKERTVHFPEQFAW 688
+A+ PL++L KR + + P P+ E++ +A++W+L + ++
Sbjct: 167 QAIAEISPLMKLVSAKRFNKSVEFPMPLKERQRRRIALQWILGEC-KSSSPKRLSDRIVK 225
Query: 689 ELLEASNNTGKVVKRKQDLHRQC 757
E++ + T K+K LHR C
Sbjct: 226 EIIAIRSKTSNCFKKKDHLHRMC 248
>SPBC11C11.03 |ndc80|ndc10, tid3|spindle pole body protein
Ndc80|Schizosaccharomyces pombe|chr 2|||Manual
Length = 624
Score = 29.1 bits (62), Expect = 0.72
Identities = 19/79 (24%), Positives = 36/79 (45%)
Frame = +2
Query: 344 KVINHVMEKGNKRLARQLVEKAFENIKRKQIERYHLASTPEEKAKIELNPKDILYKAVEN 523
++IN + A Q++E+ + ++R +++ S + K + N L +E
Sbjct: 283 ELINQIKSAEELDSAIQVLEERYRTMQRDEVKFQSAMSGMKSKMESRTNLMKQLQVNIEE 342
Query: 524 CKPLLQLQPIKRGGITYQV 580
+ LQL KR + YQV
Sbjct: 343 KESQLQLLKEKRDSLKYQV 361
>SPBC13E7.05 |gpi14||pig-M|Schizosaccharomyces pombe|chr 2|||Manual
Length = 815
Score = 28.3 bits (60), Expect = 1.3
Identities = 23/66 (34%), Positives = 34/66 (51%), Gaps = 4/66 (6%)
Frame = -1
Query: 484 LYFGF--FFWCRSQMVSFN--LFPFDIFECFLNELTSQSFVTFFHHMVDDFINERIKVNG 317
LY+ F F +S ++S++ LF F + F+ L SQSF+ V DFIN +
Sbjct: 171 LYYCFVQFMEVKSALISYDRSLFKFYPIDLFVL-LLSQSFICIIAFGVSDFIN--FQKEE 227
Query: 316 RRFRNR 299
R FR +
Sbjct: 228 RNFRGK 233
>SPAC144.05 |||ATP-dependent DNA helicase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1375
Score = 27.9 bits (59), Expect = 1.7
Identities = 28/114 (24%), Positives = 52/114 (45%)
Frame = +2
Query: 89 NVLIFFYRRLKNSSFNSSTRTLSKLQIRNYAKATSFPDYYQNPIFRKEDQVKLIADSDLK 268
N I +Y++L+ S + +S + + NY K + N + K +I + ++
Sbjct: 963 NSRIEYYKQLQEISDSLMPPPVSNISLNNYVKDDEKKQKFLNSVIIK---ASVILEKEIS 1019
Query: 269 NRATVPVKPPTVSETSSVYFDPLVNKVINHVMEKGNKRLARQLVEKAFENIKRK 430
K S+T++V LVN+ I+ + G+ L R+L E+ N +RK
Sbjct: 1020 E------KQDEASQTTNV--AELVNQKISEMNIPGHIHLLRELEEEK-SNTQRK 1064
>SPAC4A8.11c |fas2|lsd1|fatty acid synthase alpha subunit Lsd1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1842
Score = 27.5 bits (58), Expect = 2.2
Identities = 10/38 (26%), Positives = 20/38 (52%)
Frame = +2
Query: 440 RYHLASTPEEKAKIELNPKDILYKAVENCKPLLQLQPI 553
+YH+ P EK + K + + ++NCK ++ P+
Sbjct: 448 QYHIDHCPAEKGETYQLAKTLGQQLIDNCKSVIDAPPV 485
>SPBP8B7.09c |||karyopherin|Schizosaccharomyces pombe|chr 2|||Manual
Length = 978
Score = 27.5 bits (58), Expect = 2.2
Identities = 14/37 (37%), Positives = 22/37 (59%), Gaps = 3/37 (8%)
Frame = +2
Query: 305 SETSSVYFDPLVNKVI---NHVMEKGNKRLARQLVEK 406
+E + VYFDPL+N ++ N V E ++ + LV K
Sbjct: 829 TEENQVYFDPLINSILHFANLVGEPATQKSSIALVSK 865
>SPBC1709.01 |chs2|SPBC1734.17|chitin synthase homolog
Chs2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 926
Score = 27.5 bits (58), Expect = 2.2
Identities = 16/44 (36%), Positives = 23/44 (52%), Gaps = 1/44 (2%)
Frame = +2
Query: 86 SNVLIFFYRR-LKNSSFNSSTRTLSKLQIRNYAKATSFPDYYQN 214
+NVL Y LKN S ++ T +Q Y K S+ D+YQ+
Sbjct: 804 ANVLNSVYNSSLKNFSSSNDTSVYHDVQDHCYRKPQSYEDHYQD 847
>SPAC1805.06c |hem2||porphobilinogen synthase Hem2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 329
Score = 27.1 bits (57), Expect = 2.9
Identities = 18/49 (36%), Positives = 23/49 (46%)
Frame = +2
Query: 587 PITEKRSLFLAIKWLLETTDEKERTVHFPEQFAWELLEASNNTGKVVKR 733
PIT K SL I ++ E D KE P QF W + G +VK+
Sbjct: 26 PIT-KSSLMYPI-FISEIDDAKEAIDSMPNQFRWGVNRLEEFLGPLVKK 72
>SPAC32A11.04c |tif212|tif22, SPAC6B12.17c|translation initiation
factor eIF2 beta subunit|Schizosaccharomyces pombe|chr
1|||Manual
Length = 321
Score = 27.1 bits (57), Expect = 2.9
Identities = 15/54 (27%), Positives = 22/54 (40%)
Frame = +2
Query: 401 EKAFENIKRKQIERYHLASTPEEKAKIELNPKDILYKAVENCKPLLQLQPIKRG 562
+K EN+ R+ I Y T + I I + E C + +Q IK G
Sbjct: 256 QKQIENVLRRYIVEYVTCKTCKSPDTILTKENRIFFMTCEACGSVRSVQAIKTG 309
>SPBC26H8.08c |grn1||GTPase Grn1 |Schizosaccharomyces pombe|chr
2|||Manual
Length = 470
Score = 27.1 bits (57), Expect = 2.9
Identities = 18/54 (33%), Positives = 27/54 (50%), Gaps = 6/54 (11%)
Frame = +2
Query: 413 ENIKRKQIERYHLASTPEEKA------KIELNPKDILYKAVENCKPLLQLQPIK 556
E + K +ER LAS+ EEK KI+L P ++L K V + P++
Sbjct: 176 EGTRSKDVERQVLASSAEEKRLIFVINKIDLVPSEVLNKWVTYLRNFFPTIPMR 229
>SPAC1006.04c |mcp3|mug7|sequence orphan|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 952
Score = 26.2 bits (55), Expect = 5.1
Identities = 16/52 (30%), Positives = 26/52 (50%)
Frame = +2
Query: 335 LVNKVINHVMEKGNKRLARQLVEKAFENIKRKQIERYHLASTPEEKAKIELN 490
LV+K N + +K N ARQL++K+ + + + + EEK I N
Sbjct: 264 LVSKHNNALEDKFNSEAARQLLQKSLSIVASNLKQAENKTISYEEKLSIAQN 315
>SPCC306.09c |cap1|cap|adenylyl cyclase-associated protein
Cap1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 551
Score = 25.8 bits (54), Expect = 6.7
Identities = 17/56 (30%), Positives = 28/56 (50%), Gaps = 5/56 (8%)
Frame = +2
Query: 206 YQNPIFRKEDQV-----KLIADSDLKNRATVPVKPPTVSETSSVYFDPLVNKVINH 358
++NP RK K+ + + K T PVKPP + ++ +F + N+V NH
Sbjct: 361 HKNPNLRKTGPTPGPKPKIKSSAPSKPAETAPVKPPRIELENTKWF--VENQVDNH 414
>SPAC4G8.03c |||RNA-binding protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 780
Score = 25.8 bits (54), Expect = 6.7
Identities = 9/35 (25%), Positives = 21/35 (60%)
Frame = +2
Query: 341 NKVINHVMEKGNKRLARQLVEKAFENIKRKQIERY 445
N ++ HV+E + +++EK F NI + ++++
Sbjct: 658 NYLVQHVLELNIQPYTERIIEKFFGNICKLSLQKF 692
>SPAC22G7.06c |ura1||carbamoyl-phosphate synthase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2244
Score = 25.4 bits (53), Expect = 8.9
Identities = 12/32 (37%), Positives = 18/32 (56%)
Frame = -1
Query: 442 SFNLFPFDIFECFLNELTSQSFVTFFHHMVDD 347
+F++ E ++ELTS+S FFH DD
Sbjct: 1605 NFSVIASSTNEVTISELTSESGCLFFHFEKDD 1636
>SPAC631.02 |||bromodomain protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 727
Score = 25.4 bits (53), Expect = 8.9
Identities = 25/85 (29%), Positives = 41/85 (48%), Gaps = 2/85 (2%)
Frame = +2
Query: 263 LKNRA-TVPVKPPTVSETSSV-YFDPLVNKVINHVMEKGNKRLARQLVEKAFENIKRKQI 436
+K+RA + V + S + SV + P+ ++ N + E+ N A QL A I R +
Sbjct: 554 MKSRARSSSVSRRSRSRSLSVDIYPPITYEMQNELAEQCNYLSADQLSHVA--EILRAAL 611
Query: 437 ERYHLASTPEEKAKIELNPKDILYK 511
HL +T E + + P D+ YK
Sbjct: 612 P--HLRNTDEIEIDVSAMPPDVFYK 634
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,025,272
Number of Sequences: 5004
Number of extensions: 63083
Number of successful extensions: 228
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 223
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 227
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 363302114
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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