Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fmgV16d09f
         (785 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC2G5.05 |||transketolase |Schizosaccharomyces pombe|chr 2|||M...   116   5e-27
SPAC26F1.03 |pda1||pyruvate dehydrogenase e1 component alpha sub...    44   3e-05
SPBC24C6.09c |||phosphoketolase |Schizosaccharomyces pombe|chr 2...    38   0.002
SPBC725.04 |||oxalyl-CoA decarboxylase |Schizosaccharomyces pomb...    27   3.0  
SPAC227.18 |lys3|SPAC2F7.01|saccharopine dehydrogenase [NAD+, L-...    26   7.0  

>SPBC2G5.05 |||transketolase |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 685

 Score =  116 bits (278), Expect = 5e-27
 Identities = 80/232 (34%), Positives = 119/232 (51%), Gaps = 16/232 (6%)
 Frame = +3

Query: 135 NKLRIDSIVATNASKSGHPTSCASMAEIMSVLFFHTMRYKISAPRDASADRFILSKGHAA 314
           N +R  ++  T  +KSGHP +   +A    VLF   M++  + P+  + DRFILS GHA 
Sbjct: 15  NTIRTLAVDTTAHAKSGHPGAPMGLAPAAHVLFSRIMKFNPAHPKWLNRDRFILSNGHAC 74

Query: 315 PILYAAWAEAGL-FPLDELKNLRKLDSDLEGHP---TPRLNFVDVGTGSLGQGLAVAAGM 482
            + Y      G    +++LK  R++ S   GHP    P LN ++ G G LGQG+A A G+
Sbjct: 75  VLQYIMCHLLGYKLTIEDLKQFRQVGSKTPGHPETHNPDLN-IETGAGPLGQGIASAVGL 133

Query: 483 AYVGKYFDQAPYR----------VYCLVGDGEAAEGSIWESLHFASHYKLDNLVVIFDVN 632
           A +GK    A Y            +C +GDG   EG   E+   A H KL NL+ ++D N
Sbjct: 134 A-IGKAHSAAVYNKPGFDLFSNYTFCFLGDGCLQEGVSSEACSLAGHLKLSNLIAVWDNN 192

Query: 633 RLGQSEPTSLQHQLEVYDARLKAFGLNSL-VVDGH-DVTELVKAFDEAASVT 782
           ++     TS+    +V + R +A+G N + V +G  D+  + K F EA S T
Sbjct: 193 KITIDGATSMSFDEDV-EKRFEAYGWNIVRVANGDTDLDGIEKGFREAMSCT 243


>SPAC26F1.03 |pda1||pyruvate dehydrogenase e1 component alpha
           subunit Pda1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 409

 Score = 43.6 bits (98), Expect = 3e-05
 Identities = 31/115 (26%), Positives = 54/115 (46%)
 Frame = +3

Query: 438 GTGSLGQGLAVAAGMAYVGKYFDQAPYRVYCLVGDGEAAEGSIWESLHFASHYKLDNLVV 617
           G G +G  + + AG+ +  KY ++ P   + L GDG + +G  +E+ + A   KL  L V
Sbjct: 180 GNGIVGAQIPLGAGIGFAQKYLEK-PTTTFALYGDGASNQGQAFEAFNMA---KLWGLPV 235

Query: 618 IFDVNRLGQSEPTSLQHQLEVYDARLKAFGLNSLVVDGHDVTELVKAFDEAASVT 782
           IF          TS +    + +   +   +  L+V+G DV  +++A   A   T
Sbjct: 236 IFACENNKYGMGTSAERSSAMTEFYKRGQYIPGLLVNGMDVLAVLQASKFAKKYT 290


>SPBC24C6.09c |||phosphoketolase |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 825

 Score = 37.5 bits (83), Expect = 0.002
 Identities = 40/135 (29%), Positives = 58/135 (42%), Gaps = 12/135 (8%)
 Frame = +3

Query: 288 FILSKGHAAP-ILYAAWAEAGLFPL--------DELKNLRKLDSDLEGHPTPRLNFVDVG 440
           F++  GH AP IL A + E  L P         + L NL    S   G P+  +N    G
Sbjct: 125 FVVGPGHGAPAILSALFLEDSLGPFYPRYQFTKEGLNNLINTFSLPGGFPS-HVNAEVPG 183

Query: 441 TGSLGQGLAVAAGMAYVGKYFDQAPYRVYCLVGDGEAAEG---SIWESLHFASHYKLDNL 611
               G  L  A  ++Y G   D+    V C+VGDGEA  G   + W +  F    +   +
Sbjct: 184 AIHEGGELGYALSVSY-GAVLDRPDLIVTCVVGDGEAETGPTATSWHAHKFLDPAESGAV 242

Query: 612 VVIFDVNRLGQSEPT 656
           + + ++N    SE T
Sbjct: 243 IPVLELNGYKISERT 257


>SPBC725.04 |||oxalyl-CoA decarboxylase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 574

 Score = 27.1 bits (57), Expect = 3.0
 Identities = 26/100 (26%), Positives = 45/100 (45%)
 Frame = +3

Query: 333 WAEAGLFPLDELKNLRKLDSDLEGHPTPRLNFVDVGTGSLGQGLAVAAGMAYVGKYFDQA 512
           W   G   +D  + L ++      HP  RL+   + T  +G G A+A+  A+     D+ 
Sbjct: 390 WVSEGANTMDRGRQLLEVT-----HPRGRLDAGTMSTMGVGMGYAIASAFAHSS---DK- 440

Query: 513 PYRVYCLVGDGEAAEGSIWESLHFASHYKLDNLVVIFDVN 632
                 +V +G++A G     L  A   +LD LV++ + N
Sbjct: 441 -----IVVVEGDSAFGFSAMELETAIRNQLDLLVIVINNN 475


>SPAC227.18 |lys3|SPAC2F7.01|saccharopine dehydrogenase [NAD+,
           L-lysine forming] |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 368

 Score = 25.8 bits (54), Expect = 7.0
 Identities = 14/52 (26%), Positives = 22/52 (42%)
 Frame = -1

Query: 647 GLPQTVNIEDDDQVVQLVVAGKVQRLPDAALGCLSVAHQAIHPVGRLVEVLP 492
           GL   +    DD   ++   G       +A+ CL  AHQ +HP  +   + P
Sbjct: 116 GLLYDLEFLQDDNGRRVAAFGYHAGFAGSAISCLVWAHQLLHPNKQFPAIRP 167


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,778,681
Number of Sequences: 5004
Number of extensions: 53347
Number of successful extensions: 158
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 149
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 155
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 381366860
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)