Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fmgV16a08f
(801 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC1815.01 |eno101|eno1|enolase|Schizosaccharomyces pombe|chr 2... 276 3e-75
SPBPB21E7.01c |eno102|eno1, SPBPB8B6.07c, eno1|enolase |Schizosa... 256 4e-69
SPBC4B4.10c |mug77|atg5|autophagy associated protein Atg5 |Schiz... 31 0.25
SPCC1442.06 |||20S proteasome component alpha 2|Schizosaccharomy... 28 1.3
SPCC1672.03c |||guanine deaminase |Schizosaccharomyces pombe|chr... 27 2.3
SPCC569.03 |||DUF1773 family protein 4|Schizosaccharomyces pombe... 27 3.1
SPCC290.03c |nup186||nucleoporin Nup186|Schizosaccharomyces pomb... 26 5.4
SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces... 25 9.5
>SPBC1815.01 |eno101|eno1|enolase|Schizosaccharomyces pombe|chr
2|||Manual
Length = 439
Score = 276 bits (676), Expect = 3e-75
Identities = 134/220 (60%), Positives = 165/220 (75%), Gaps = 1/220 (0%)
Frame = +3
Query: 120 MVIKSIKARQIFDSRGNPTVEVDLVTELGLFRAAVPSGASTGVHEALELRDNIKSEYHGK 299
M I+ + ARQI+DSRGNPTVEVDL TE G+ RA VPSGASTG+ EALE+RD K+++ GK
Sbjct: 1 MAIQKVFARQIYDSRGNPTVEVDLTTETGIHRAIVPSGASTGIWEALEMRDGDKTKWGGK 60
Query: 300 GVLTAIKNINELIAPELTKANLEVTQQREIDELMLKLDGTENKSKLGANAILGVSLXXXX 479
GVL A+ N+N +IAP + KANL+VT Q+ DE +LKLDGTENKSKLGANAILGVS+
Sbjct: 61 GVLKAVGNVNNIIAPAVVKANLDVTDQKAADEFLLKLDGTENKSKLGANAILGVSMAICR 120
Query: 480 XXXXXXNVPLYKHLADLAG-NNDIVLPVPAFNVINGGSHAGNKLAMQEFMIFPTGASTFS 656
+PL+K++A+ G VLPVP+FNV+NGGSHAG LA QEFMI PTGA +FS
Sbjct: 121 AGAAQKKLPLWKYIAENFGTKGPYVLPVPSFNVLNGGSHAGGDLAFQEFMILPTGAPSFS 180
Query: 657 EAMRMGSEVYHHLKKIIKEKFGLDSTAVGDEGGFAPTYKT 776
EAMR G+E YH LK I K+++G + VGDEGG AP +T
Sbjct: 181 EAMRWGAETYHTLKSIAKKRYGSSAGNVGDEGGIAPDLQT 220
>SPBPB21E7.01c |eno102|eno1, SPBPB8B6.07c, eno1|enolase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 440
Score = 256 bits (626), Expect = 4e-69
Identities = 123/218 (56%), Positives = 157/218 (72%), Gaps = 1/218 (0%)
Frame = +3
Query: 126 IKSIKARQIFDSRGNPTVEVDLVTELGLFRAAVPSGASTGVHEALELRDNIKSEYHGKGV 305
I+ I +R I+DSRGNPTVEV+L TELG FR+ VPSGASTG EA ELRDN K+++ GKGV
Sbjct: 4 IQKIYSRSIYDSRGNPTVEVELTTELGTFRSMVPSGASTGEWEAKELRDNDKNKWGGKGV 63
Query: 306 LTAIKNINELIAPELTKANLEVTQQREIDELMLKLDGTENKSKLGANAILGVSLXXXXXX 485
A+ N+N +I P L K+++++T QR IDE M+KLDGT +KSKLGAN+I+GVS+
Sbjct: 64 TIAVHNVNNIIGPALVKSDIKITDQRGIDEFMIKLDGTNDKSKLGANSIVGVSMAVARAA 123
Query: 486 XXXXNVPLYKHLADLAGNNDI-VLPVPAFNVINGGSHAGNKLAMQEFMIFPTGASTFSEA 662
+PLY+++ LAG+ +PVP+FNV+NGG HAG LA QEFMI P A TFSE
Sbjct: 124 AAFLKIPLYEYIGKLAGSKTTECIPVPSFNVLNGGRHAGGDLAFQEFMIMPIKAPTFSEG 183
Query: 663 MRMGSEVYHHLKKIIKEKFGLDSTAVGDEGGFAPTYKT 776
+R GSEVYH LK + K+K+G + VGDEGG AP T
Sbjct: 184 LRWGSEVYHTLKALAKKKYGASAGNVGDEGGIAPDLTT 221
>SPBC4B4.10c |mug77|atg5|autophagy associated protein Atg5
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 261
Score = 30.7 bits (66), Expect = 0.25
Identities = 15/45 (33%), Positives = 24/45 (53%)
Frame = -3
Query: 673 PILMASLKVDAPVGKIMNSCMASLFPACDPPLITLNAGTGRTMSL 539
PI+ S + + +G+ +N + LFP+CD LI G T+ L
Sbjct: 192 PIIQTSAPLGSSLGEFLNKRLPDLFPSCDKFLIVKPVIHGITIFL 236
>SPCC1442.06 |||20S proteasome component alpha 2|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 245
Score = 28.3 bits (60), Expect = 1.3
Identities = 22/72 (30%), Positives = 32/72 (44%)
Frame = +3
Query: 156 DSRGNPTVEVDLVTELGLFRAAVPSGASTGVHEALELRDNIKSEYHGKGVLTAIKNINEL 335
D +G +VD ++A +ST LE R N + E V TAI + E
Sbjct: 140 DEKGPSLYQVDPSGTYFAWKATAIGKSSTAAKTFLEKRYNDELELDD-AVHTAILALKET 198
Query: 336 IAPELTKANLEV 371
ELT+ N+E+
Sbjct: 199 FEGELTEDNIEI 210
>SPCC1672.03c |||guanine deaminase |Schizosaccharomyces pombe|chr
3|||Manual
Length = 527
Score = 27.5 bits (58), Expect = 2.3
Identities = 9/12 (75%), Positives = 10/12 (83%)
Frame = +2
Query: 464 PSCC*GWCCQEE 499
PSCC G CC+EE
Sbjct: 490 PSCCGGHCCKEE 501
>SPCC569.03 |||DUF1773 family protein 4|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 396
Score = 27.1 bits (57), Expect = 3.1
Identities = 31/117 (26%), Positives = 58/117 (49%), Gaps = 3/117 (2%)
Frame = +3
Query: 93 LRKSSSVLKMVIKSIKARQIFDSRGNPTV-EVDLVTELGLFRAAVPSGASTGVHEALELR 269
++ +V+K I SIK ++ + +G TV + D+ + G A G T + +
Sbjct: 171 MKGEMAVMKNDIASIKG-EMAEMKGEMTVMKNDIASIKG--EMAEMKGEMTIMKSDI--- 224
Query: 270 DNIKSEY-HGKGVLTAIKNINELIAPELTKANLEVTQQRE-IDELMLKLDGTENKSK 434
D++K E KG +T +KN + + E T EVT ++ I +L K+D + +++
Sbjct: 225 DSVKGEMAEMKGEMTIMKNDIDSVKGETTTLKGEVTAMKDSISQLDRKIDLLDQRTE 281
>SPCC290.03c |nup186||nucleoporin Nup186|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1647
Score = 26.2 bits (55), Expect = 5.4
Identities = 22/98 (22%), Positives = 42/98 (42%)
Frame = +3
Query: 102 SSSVLKMVIKSIKARQIFDSRGNPTVEVDLVTELGLFRAAVPSGASTGVHEALELRDNIK 281
+S V VI++ + G P + ++ LG F + S S + ++ + +
Sbjct: 1088 TSEVTLSVIRARPGLLVKMVDGEPILTQQVIQNLGGFSSEEVSMVSRCIRSRTQIMNMLA 1147
Query: 282 SEYHGKGVLTAIKNINELIAPELTKANLEVTQQREIDE 395
+E H + K +NE +A L + N E T E+ +
Sbjct: 1148 TEIHYAASVGQNKYLNEYVA-SLIRTN-EKTHSTELSQ 1183
>SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 3699
Score = 25.4 bits (53), Expect = 9.5
Identities = 14/54 (25%), Positives = 28/54 (51%), Gaps = 1/54 (1%)
Frame = -2
Query: 356 FCKFRSNEFIDIFDCGQNSLAMIFTLDV-IS*FKSFMNTSGCTRGYSCPEQAKL 198
F + +EF +IF+C ++LA++ +S + +N +G + C AK+
Sbjct: 657 FVEILESEFPNIFECLVDNLALLHIFQFWMSNEVTSVNCTGIVLSFCCDNLAKI 710
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,319,230
Number of Sequences: 5004
Number of extensions: 65517
Number of successful extensions: 152
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 148
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 150
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 388424860
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -