Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fmgV15d07r
(963 letters)
Database: bee
438 sequences; 146,343 total letters
Searching......................................................done
Score E
Sequences producing significant alignments: (bits) Value
AB253416-1|BAE86927.1| 580|Apis mellifera alpha-glucosidase pro... 203 2e-54
D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein. 199 3e-53
AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase pro... 198 5e-53
AB253415-1|BAE86926.1| 588|Apis mellifera alpha-glucosidase pro... 144 8e-37
AF213012-1|AAG43568.1| 492|Apis mellifera acetylcholinesterase ... 25 1.4
AB181702-1|BAE06051.1| 628|Apis mellifera acetylcholinesterase ... 25 1.4
AF469010-1|AAL93136.1| 678|Apis mellifera cGMP-dependent protei... 24 2.4
DQ069332-1|AAZ32217.1| 296|Apis mellifera RNA polymerase II lar... 23 5.4
>AB253416-1|BAE86927.1| 580|Apis mellifera alpha-glucosidase protein.
Length = 580
Score = 203 bits (495), Expect = 2e-54
Identities = 102/282 (36%), Positives = 158/282 (56%)
Frame = -3
Query: 856 RAMMTEVYASIQDVVRYFGEGDLKGAQIPFNFDLITDVDASSSAADIKRAVDKFLTYKPV 677
R ++TE Y ++Y+ G + +PFNF I D++ S+A+D K+ +D+++ P
Sbjct: 287 RMILTEAYTEFNLTIKYYKSG----STVPFNFMFIADLNNQSTASDFKQLIDRWVANVPN 342
Query: 676 DQTANWVVGNHDNSRMATRYGASLVDGINMLVLLLPGVAVTYMGEEIGLEDGYVSWEDTV 497
NWV GNHDN R+A+R+G D I ML L LPG+ V Y G+EIG+ED + ++++TV
Sbjct: 343 GSVTNWVSGNHDNHRVASRFGRQRGDEIVMLTLTLPGIGVVYNGDEIGMEDRWFTYQETV 402
Query: 496 DPSGCNTNDPIKYVESSRDPERTPFHWNPEKNAGFSTADKTWLPMAEGYETLNVEVQKAS 317
DP+GCN P KY SRDPERTP+ W+ +AGFS +KTWLP+ E Y++LN+ QK
Sbjct: 403 DPAGCNAG-PAKYYLKSRDPERTPYQWDNSTSAGFSQTNKTWLPVNENYKSLNLAAQKRE 461
Query: 316 ERSHLKVYKALSDLRQENTFRYGRYESLALNQDIFVFKRWLNDVIYLVVVNMRDVEHNID 137
SH +K+LS L+++ G E ++ + KR L + +V++N ++
Sbjct: 462 YYSHYVAFKSLSYLKKQPVIANGSLEVDVIDGRVLSVKRELGNDTVIVMMNFSKNPVTVN 521
Query: 136 LTYFENVSGNVAVSIRSVNSPKNEGDTFDAKSLPVVGFEGLV 11
LT + V + V S + G+ S+ + G V
Sbjct: 522 LTKLHPPADLVVYACNVVGSGLSHGNWIYPASMTIPGSNSAV 563
Score = 31.9 bits (69), Expect = 0.009
Identities = 10/25 (40%), Positives = 19/25 (76%)
Frame = -2
Query: 956 LXHIHTKDHNDTYYMVYQWRDVFDE 882
L H++T+D ++TY ++ WR++ DE
Sbjct: 253 LVHLYTRDQSETYDVLRSWRNLMDE 277
>D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein.
Length = 567
Score = 199 bits (485), Expect = 3e-53
Identities = 99/277 (35%), Positives = 148/277 (53%), Gaps = 1/277 (0%)
Frame = -3
Query: 850 MMTEVYASIQDVVRYFGEGDLKGAQIPFNFDLITDVDASSSAADIKRAVDKFLTYKPVDQ 671
M+ E Y ++ ++Y+ G A PFNF I +V S+++D K+ VD ++TY P
Sbjct: 283 MLIEAYTNLSMTMKYYDYG----ADFPFNFAFIKNVSRDSNSSDFKKLVDNWMTYMPPSG 338
Query: 670 TANWVVGNHDNSRMATRYGASLVDGINMLVLLLPGVAVTYMGEEIGLEDGYVSWEDTVDP 491
NWV GNHD R+ +R+G I + LLLPGVAV Y G+EIG+ D Y+SWEDT DP
Sbjct: 339 IPNWVPGNHDQLRLVSRFGEEKARMITTMSLLLPGVAVNYYGDEIGMSDTYISWEDTQDP 398
Query: 490 SGCNTNDPIKYVESSRDPERTPFHWNPEKNAGFSTADKTWLPMAEGYETLNVEVQKASER 311
GC Y SRDP RTPF W+ +AGFS++ TWL + E Y+T+N+ +K +
Sbjct: 399 QGCGAGKE-NYQTMSRDPARTPFQWDDSVSAGFSSSSNTWLRVNENYKTVNLAAEKKDKN 457
Query: 310 SHLKVYKALSDLRQENTFRYGRYESLALNQDIFVFKRWLNDVIYL-VVVNMRDVEHNIDL 134
S ++K + L++ F+ + LN ++F F R D L ++N + E +DL
Sbjct: 458 SFFNMFKKFASLKKSPYFKEANLNTRMLNDNVFAFSRETEDNGSLYAILNFSNEEQIVDL 517
Query: 133 TYFENVSGNVAVSIRSVNSPKNEGDTFDAKSLPVVGF 23
F NV + + + NS + + +GF
Sbjct: 518 KAFNNVPKKLNMFYNNFNSDIKSISNNEQVKVSALGF 554
Score = 28.3 bits (60), Expect = 0.11
Identities = 12/27 (44%), Positives = 18/27 (66%)
Frame = -2
Query: 962 DYLXHIHTKDHNDTYYMVYQWRDVFDE 882
+Y I+T D +TY +V ++RDV DE
Sbjct: 250 EYTLKIYTHDIPETYNVVRKFRDVLDE 276
>AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase protein.
Length = 567
Score = 198 bits (484), Expect = 5e-53
Identities = 98/277 (35%), Positives = 148/277 (53%), Gaps = 1/277 (0%)
Frame = -3
Query: 850 MMTEVYASIQDVVRYFGEGDLKGAQIPFNFDLITDVDASSSAADIKRAVDKFLTYKPVDQ 671
M+ E Y ++ ++Y+ G A PFNF I +V S+++D K+ +D ++TY P
Sbjct: 283 MLIEAYTNLSMTMKYYDYG----ADFPFNFAFIKNVSRDSNSSDFKKLIDNWMTYMPPSG 338
Query: 670 TANWVVGNHDNSRMATRYGASLVDGINMLVLLLPGVAVTYMGEEIGLEDGYVSWEDTVDP 491
NWV GNHD R+ +R+G I + LLLPGVAV Y G+EIG+ D Y+SWEDT DP
Sbjct: 339 IPNWVPGNHDQLRLVSRFGEEKARMITTMSLLLPGVAVNYYGDEIGMSDTYISWEDTQDP 398
Query: 490 SGCNTNDPIKYVESSRDPERTPFHWNPEKNAGFSTADKTWLPMAEGYETLNVEVQKASER 311
GC Y SRDP RTPF W+ +AGFS++ TWL + E Y+T+N+ +K +
Sbjct: 399 QGCGAGKE-NYQTMSRDPARTPFQWDDSVSAGFSSSSNTWLRVNENYKTVNLAAEKKDKN 457
Query: 310 SHLKVYKALSDLRQENTFRYGRYESLALNQDIFVFKRWLNDVIYL-VVVNMRDVEHNIDL 134
S ++K + L++ F+ + LN ++F F R D L ++N + E +DL
Sbjct: 458 SFFNMFKKFASLKKSPYFKEANLNTRMLNDNVFAFSRETEDNGSLYAILNFSNEEQIVDL 517
Query: 133 TYFENVSGNVAVSIRSVNSPKNEGDTFDAKSLPVVGF 23
F NV + + + NS + + +GF
Sbjct: 518 KAFNNVPKKLNMFYNNFNSDIKSISNNEQVKVSALGF 554
Score = 28.3 bits (60), Expect = 0.11
Identities = 12/27 (44%), Positives = 18/27 (66%)
Frame = -2
Query: 962 DYLXHIHTKDHNDTYYMVYQWRDVFDE 882
+Y I+T D +TY +V ++RDV DE
Sbjct: 250 EYTLKIYTHDIPETYNVVRKFRDVLDE 276
>AB253415-1|BAE86926.1| 588|Apis mellifera alpha-glucosidase protein.
Length = 588
Score = 144 bits (350), Expect = 8e-37
Identities = 90/286 (31%), Positives = 143/286 (50%), Gaps = 6/286 (2%)
Frame = -3
Query: 850 MMTEVYASIQDVVRYFGEGDLKGAQIPFNFDLITDVDASSSAADIKRAVDKFLTYKPVDQ 671
++TE Y+S+++ ++Y+ G + +PFNF ITD ++SS+ K +D ++ P +
Sbjct: 296 LLTEAYSSLENTLKYYEVG----SNVPFNFKFITDANSSSTPEQFKVIIDNWIKGTPQNN 351
Query: 670 TANWVVGNHDNSRMATRYGASLVDGINMLVLLLPGVAVTYMGEEIGLEDGYVSWEDTVDP 491
NWV+GNHD R+ TRY D + ML ++LPGVAVTY GEEIG+ D
Sbjct: 352 VPNWVMGNHDRVRVGTRY-PGRADHMIMLEMILPGVAVTYYGEEIGMVD----------- 399
Query: 490 SGCNTNDPIKYVESSRDPERTPFHWNPEKNAGFS-----TADKTWLPMAEGYET-LNVEV 329
NT Y RD RTPF W+ NAGFS +K WLP+ Y++ LN+E
Sbjct: 400 ---NTT---IYKYDVRDGCRTPFQWDNSINAGFSKIAENLLEKNWLPVHTSYKSGLNLEQ 453
Query: 328 QKASERSHLKVYKALSDLRQENTFRYGRYESLALNQDIFVFKRWLNDVIYLVVVNMRDVE 149
+K SH +Y L+ LR+ + + G + LN+ + R + +++N
Sbjct: 454 EKKDSISHYHLYTNLTALRKRDVLKKGNFTIEILNKTVLAVVRQSEEEAVSLLINFSKNN 513
Query: 148 HNIDLTYFENVSGNVAVSIRSVNSPKNEGDTFDAKSLPVVGFEGLV 11
+D++ N N + SVNS T + ++ + G ++
Sbjct: 514 TIVDISKLVNKRNNAKIYTSSVNSNLTVNQTVNPVAINIPGDTSII 559
Score = 30.3 bits (65), Expect = 0.027
Identities = 12/24 (50%), Positives = 15/24 (62%)
Frame = -2
Query: 956 LXHIHTKDHNDTYYMVYQWRDVFD 885
L H TKD +TY +V +WRD D
Sbjct: 260 LNHTLTKDQPETYELVKEWRDFVD 283
>AF213012-1|AAG43568.1| 492|Apis mellifera acetylcholinesterase
protein.
Length = 492
Score = 24.6 bits (51), Expect = 1.4
Identities = 7/24 (29%), Positives = 16/24 (66%)
Frame = -2
Query: 947 IHTKDHNDTYYMVYQWRDVFDEXQ 876
I ++ TY+++Y + D+F++ Q
Sbjct: 376 IGNNENEGTYFILYDFNDIFEKDQ 399
Score = 23.0 bits (47), Expect = 4.1
Identities = 9/23 (39%), Positives = 12/23 (52%)
Frame = +2
Query: 371 PGLVSGRESSVFLGVPMERSPFG 439
P V +E VF G+P + P G
Sbjct: 51 PRTVLDKEVHVFYGIPFAKPPIG 73
>AB181702-1|BAE06051.1| 628|Apis mellifera acetylcholinesterase
protein.
Length = 628
Score = 24.6 bits (51), Expect = 1.4
Identities = 7/24 (29%), Positives = 16/24 (66%)
Frame = -2
Query: 947 IHTKDHNDTYYMVYQWRDVFDEXQ 876
I ++ TY+++Y + D+F++ Q
Sbjct: 376 IGNNENEGTYFILYDFNDIFEKDQ 399
Score = 23.0 bits (47), Expect = 4.1
Identities = 9/23 (39%), Positives = 12/23 (52%)
Frame = +2
Query: 371 PGLVSGRESSVFLGVPMERSPFG 439
P V +E VF G+P + P G
Sbjct: 51 PRTVLDKEVHVFYGIPFAKPPIG 73
>AF469010-1|AAL93136.1| 678|Apis mellifera cGMP-dependent protein
kinase foraging protein.
Length = 678
Score = 23.8 bits (49), Expect = 2.4
Identities = 7/24 (29%), Positives = 16/24 (66%)
Frame = +3
Query: 378 LSAVENPAFFSGFQWKGVLSGSLD 449
+S ++ +F GF W+G+ + +L+
Sbjct: 617 ISEIQKHKWFDGFNWEGLRARTLE 640
>DQ069332-1|AAZ32217.1| 296|Apis mellifera RNA polymerase II large
subunit protein.
Length = 296
Score = 22.6 bits (46), Expect = 5.4
Identities = 8/23 (34%), Positives = 15/23 (65%)
Frame = -3
Query: 829 SIQDVVRYFGEGDLKGAQIPFNF 761
+I V+ G+ +++G +IPF F
Sbjct: 241 NISQVIACVGQQNVEGKRIPFGF 263
Database: bee
Posted date: Oct 23, 2007 1:17 PM
Number of letters in database: 146,343
Number of sequences in database: 438
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 270,764
Number of Sequences: 438
Number of extensions: 6300
Number of successful extensions: 31
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 16
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 23
length of database: 146,343
effective HSP length: 58
effective length of database: 120,939
effective search space used: 31686018
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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