Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fmgV14l09f
(599 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC1604.21c |ptr3|uba1, SPBC211.09|ubiquitin activating enzyme ... 130 1e-31
SPAC4C5.04 |rad31|uba4|SUMO E1-like activator enzyme Rad31|Schiz... 60 2e-10
SPBC16H5.03c |fub2|uba2|SUMO E1-like activator enzyme Fub2|Schiz... 59 6e-10
SPAC323.06c |uba5||NEDD8 activating enzyme |Schizosaccharomyces ... 37 0.003
SPAC1A6.10 ||SPAC30D11.15c|Moeb/ThiF domain|Schizosaccharomyces ... 36 0.006
SPBC6B1.05c |||ubiquitin-like conjugating enzyme|Schizosaccharom... 30 0.22
SPAC23D3.13c |||guanyl-nucleotide exchange factor|Schizosaccharo... 27 1.6
SPAC212.11 |tlh1||RecQ type DNA helicase|Schizosaccharomyces pom... 27 2.8
SPBCPT2R1.08c |tlh2||RecQ type DNA helicase Tlh1|Schizosaccharom... 27 2.8
SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 27 2.8
SPBC1711.04 |||methylenetetrahydrofolate reductase |Schizosaccha... 26 4.8
SPBC4B4.04 |||translation initiation factor eIF2A |Schizosacchar... 25 8.5
>SPBC1604.21c |ptr3|uba1, SPBC211.09|ubiquitin activating enzyme
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1012
Score = 130 bits (315), Expect = 1e-31
Identities = 66/122 (54%), Positives = 89/122 (72%)
Frame = +3
Query: 231 EDEIDESLYSRQLYVLGHDAMRRMASSDVLISGLGGLGVEIAKNVILGGVKSVTLHDDKN 410
++ IDE LYSRQLYVLGH+AM++M+ S+VLI G GLGVEIAKNV L GVKSVTL+D +
Sbjct: 12 QNTIDEGLYSRQLYVLGHEAMKQMSQSNVLIIGCKGLGVEIAKNVCLAGVKSVTLYDPQP 71
Query: 411 CTVADLSSQFYLSETVIGQNRALTSCEQLSELNHYVPTTAYTGPLTEDFLRKFRVVVLTG 590
+ DLSSQ++L+E IG RA + +L+ELN YVP + L+ ++L+ F+ VV+T
Sbjct: 72 TRIEDLSSQYFLTEDDIGVPRAKVTVSKLAELNQYVPVSV-VDELSTEYLKNFKCVVVTE 130
Query: 591 AS 596
S
Sbjct: 131 TS 132
>SPAC4C5.04 |rad31|uba4|SUMO E1-like activator enzyme
Rad31|Schizosaccharomyces pombe|chr 1|||Manual
Length = 307
Score = 60.5 bits (140), Expect = 2e-10
Identities = 42/130 (32%), Positives = 66/130 (50%), Gaps = 3/130 (2%)
Frame = +3
Query: 207 MANNGARVEDEIDESLYSRQLYVLGHDAMRRMASSDVLISGLGGLGVEIAKNVILGGVKS 386
M N+ E EI +LY RQ+ + G +A + + S VL+ L EIAKN++L G+
Sbjct: 1 MGNHNINAE-EI--ALYDRQIRLWGFNAQQALKQSRVLLITASPLANEIAKNLVLSGIGK 57
Query: 387 VTLHDDKNCTVADLSSQFYLSETVIGQNRALTSCEQLSELNHYV---PTTAYTGPLTEDF 557
+ + D D+ QF++ + IGQ RA ++L ELN V T+ + E
Sbjct: 58 LCVLDSMTVYEKDVEEQFFIEASDIGQLRANVFKKKLHELNPLVEIDTDTSLISEIDEGK 117
Query: 558 LRKFRVVVLT 587
+ KF +V+ T
Sbjct: 118 ISKFSMVIAT 127
>SPBC16H5.03c |fub2|uba2|SUMO E1-like activator enzyme
Fub2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 628
Score = 58.8 bits (136), Expect = 6e-10
Identities = 29/90 (32%), Positives = 50/90 (55%)
Frame = +3
Query: 285 DAMRRMASSDVLISGLGGLGVEIAKNVILGGVKSVTLHDDKNCTVADLSSQFYLSETVIG 464
+A+R S+ VL+ G GG+G E+ KN+++ GVK V + D +++L+ QF + +
Sbjct: 18 EALRNFKSAKVLLVGAGGIGCELLKNLLMSGVKEVHIIDLDTIDLSNLNRQFLFRKKHVK 77
Query: 465 QNRALTSCEQLSELNHYVPTTAYTGPLTED 554
Q +A+ + + S N V AY + ED
Sbjct: 78 QPKAIVAAKTASSFNPNVKLEAYHANIKED 107
>SPAC323.06c |uba5||NEDD8 activating enzyme |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 500
Score = 36.7 bits (81), Expect = 0.003
Identities = 22/85 (25%), Positives = 39/85 (45%)
Frame = +3
Query: 255 YSRQLYVLGHDAMRRMASSDVLISGLGGLGVEIAKNVILGGVKSVTLHDDKNCTVADLSS 434
Y RQ+ + + + S V + +G E KN+IL G+ S + DD + +
Sbjct: 10 YDRQVRLWKAEGQNAIEKSHVCLLYANTVGCEALKNLILPGIGSFAVVDDTSVDFSMDGM 69
Query: 435 QFYLSETVIGQNRALTSCEQLSELN 509
F++ G++RA + L +LN
Sbjct: 70 NFFIQYDQEGKSRARCTASLLQQLN 94
>SPAC1A6.10 ||SPAC30D11.15c|Moeb/ThiF domain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 485
Score = 35.5 bits (78), Expect = 0.006
Identities = 19/106 (17%), Positives = 46/106 (43%)
Frame = +3
Query: 213 NNGARVEDEIDESLYSRQLYVLGHDAMRRMASSDVLISGLGGLGVEIAKNVILGGVKSVT 392
+ G ++ + +R G D M R+ +S V++ G GG+G + + GV+ +
Sbjct: 95 SKGVPYDENLIREQLARNYAFFGEDGMERLRNSFVIVVGCGGVGSWVINMLARSGVQKIR 154
Query: 393 LHDDKNCTVADLSSQFYLSETVIGQNRALTSCEQLSELNHYVPTTA 530
+ D +++ L+ + +G + L + + + ++ A
Sbjct: 155 IVDFDQVSLSSLNRHSIATLQDVGTPKTLAIKKAIKKFAPWIEVDA 200
>SPBC6B1.05c |||ubiquitin-like conjugating
enzyme|Schizosaccharomyces pombe|chr 2|||Manual
Length = 649
Score = 30.3 bits (65), Expect = 0.22
Identities = 14/35 (40%), Positives = 22/35 (62%)
Frame = +3
Query: 297 RMASSDVLISGLGGLGVEIAKNVILGGVKSVTLHD 401
R+ +S L+ G G LG +A+N++ GV+ VT D
Sbjct: 332 RIQNSKCLLLGAGTLGCGVARNLLSWGVRHVTFVD 366
>SPAC23D3.13c |||guanyl-nucleotide exchange
factor|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1616
Score = 27.5 bits (58), Expect = 1.6
Identities = 10/30 (33%), Positives = 19/30 (63%)
Frame = +2
Query: 29 CCFKQFEENFFLIVNATKILAKLCAFVPDV 118
CC K+ E + ++ +N+ ++LA A PD+
Sbjct: 64 CCAKKIERHIYISLNSIQLLAINDALSPDI 93
>SPAC212.11 |tlh1||RecQ type DNA helicase|Schizosaccharomyces
pombe|chr 1||Partial|Manual
Length = 1887
Score = 26.6 bits (56), Expect = 2.8
Identities = 31/109 (28%), Positives = 49/109 (44%), Gaps = 7/109 (6%)
Frame = +3
Query: 93 NCARSCPMSSAEVAD-----NSVDPPAKKR--KLNTGEAHSNNSAMANNGARVEDEIDES 251
N + P+S +E+++ +SV P KK NT + ++ + DE
Sbjct: 1637 NTTSTTPVSLSELSEITLFPSSVSPTWKKSFGNANTNLKYGLEDMSLSHRRGHKRTYDEH 1696
Query: 252 LYSRQLYVLGHDAMRRMASSDVLISGLGGLGVEIAKNVILGGVKSVTLH 398
L + Q V HD M R+ S +SG+ G+G+ I G V S T+H
Sbjct: 1697 LNNVQQGV-NHD-MNRVHGSVGGMSGIVGIGIGIGDGDGDGDVDSRTIH 1743
>SPBCPT2R1.08c |tlh2||RecQ type DNA helicase Tlh1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1919
Score = 26.6 bits (56), Expect = 2.8
Identities = 31/109 (28%), Positives = 49/109 (44%), Gaps = 7/109 (6%)
Frame = +3
Query: 93 NCARSCPMSSAEVAD-----NSVDPPAKKR--KLNTGEAHSNNSAMANNGARVEDEIDES 251
N + P+S +E+++ +SV P KK NT + ++ + DE
Sbjct: 1637 NTTSTTPVSLSELSEITLFPSSVSPTWKKSFGNANTNLKYGLEDMSLSHRRGHKRTYDEH 1696
Query: 252 LYSRQLYVLGHDAMRRMASSDVLISGLGGLGVEIAKNVILGGVKSVTLH 398
L + Q V HD M R+ S +SG+ G+G+ I G V S T+H
Sbjct: 1697 LNNVQQGV-NHD-MNRVHGSVGGMSGIVGIGIGIGDGDGDGDVDSRTIH 1743
>SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit
Arp9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 523
Score = 26.6 bits (56), Expect = 2.8
Identities = 11/20 (55%), Positives = 13/20 (65%)
Frame = -2
Query: 433 EERSATVQFLSSCKVTDFTP 374
EE++ F S CKV DFTP
Sbjct: 87 EEKNMGSSFHSECKVDDFTP 106
>SPBC1711.04 |||methylenetetrahydrofolate reductase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 320
Score = 25.8 bits (54), Expect = 4.8
Identities = 16/69 (23%), Positives = 25/69 (36%)
Frame = +3
Query: 309 SDVLISGLGGLGVEIAKNVILGGVKSVTLHDDKNCTVADLSSQFYLSETVIGQNRALTSC 488
SDV+I G+ + N++ G + +KN A L + IG+
Sbjct: 244 SDVIICGVPSANYKFPSNLVRDGAVCICFSSEKNFDAASLKEHAGIYVPSIGKVTIAMLL 303
Query: 489 EQLSELNHY 515
L L Y
Sbjct: 304 RNLIRLTSY 312
>SPBC4B4.04 |||translation initiation factor eIF2A
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 576
Score = 25.0 bits (52), Expect = 8.5
Identities = 15/47 (31%), Positives = 23/47 (48%), Gaps = 2/47 (4%)
Frame = +3
Query: 315 VLISGLGGLGVEIAKNVILGGVKSVTLHDDKNCTVADLS--SQFYLS 449
+L++G G L I +K +T + NCT + S SQF L+
Sbjct: 324 ILLAGFGNLQGSIDIFDAANNMKKITTVEAANCTYCEFSPDSQFLLT 370
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,535,877
Number of Sequences: 5004
Number of extensions: 51598
Number of successful extensions: 171
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 168
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 171
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 262236260
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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