Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fmgV14f09f
(596 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC1683.07 |mal1||alpha-glucosidase Mal1 |Schizosaccharomyces p... 113 2e-26
SPAC23D3.14c |aah2||alpha-amylase homolog Aah2|Schizosaccharomyc... 52 6e-08
SPCC63.02c |aah3||alpha-amylase homolog Aah3|Schizosaccharomyces... 44 1e-05
SPCC11E10.09c ||SPCC188.01c|alpha-amylase homolog |Schizosacchar... 41 1e-04
SPBC16A3.13 |meu7|aah4|alpha-amylase homolog Aah4|Schizosaccharo... 40 2e-04
SPAC25H1.09 |mde5|meu30, SPAC4A8.01|alpha-amylase homolog Mde5|S... 40 3e-04
SPAC27E2.01 |||alpha-amylase homolog |Schizosaccharomyces pombe|... 40 4e-04
SPBC32H8.13c |mok12||alpha-1,3-glucan synthase Mok12|Schizosacch... 37 0.002
SPCC757.12 |||alpha-amylase homolog |Schizosaccharomyces pombe|c... 29 0.52
SPBC354.07c |||oxysterol binding protein |Schizosaccharomyces po... 27 2.7
SPBC106.04 |ada1||adenosine deaminase Ada1 |Schizosaccharomyces ... 26 3.6
SPBC119.17 ||SPBC577.01|metallopeptidase|Schizosaccharomyces pom... 25 6.3
SPAP27G11.03 |||D123 family|Schizosaccharomyces pombe|chr 1|||Ma... 25 6.3
SPAC23D3.06c |nup146||nucleoporin Nup146|Schizosaccharomyces pom... 25 6.3
SPBC11B10.07c |||CDC50 domain protein|Schizosaccharomyces pombe|... 25 6.3
SPAC1834.08 |mak1|phk3|histidine kinase Mak1|Schizosaccharomyces... 25 8.4
SPAC31G5.05c |||ribulose phosphate 3-epimerase |Schizosaccharomy... 25 8.4
>SPBC1683.07 |mal1||alpha-glucosidase Mal1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 579
Score = 113 bits (271), Expect = 2e-26
Identities = 51/151 (33%), Positives = 85/151 (56%), Gaps = 1/151 (0%)
Frame = +2
Query: 131 PTEVIQLDWWKNCVLYQIYPRSFXXXXXXXXXXXXXXXXELKHFVDAGVDAIWMSPIFES 310
P+E I+ +WW+ +YQIYP SF ++ + V++IW+ PI+ S
Sbjct: 5 PSEKIKPNWWRETSVYQIYPASFKDSNGDGFGDLEGIISKVDYLKALNVESIWLCPIYPS 64
Query: 311 PMVDFGYDISNFYDIHYEYGTMEDFEELLDKAHELGLKVLLDFVPNHASNESEYFIKSEA 490
P+ D GYD+S++ I YGT+ED + L+ HE +K+++D V NH S++ E+F +S +
Sbjct: 65 PLKDMGYDVSDYKQIDSRYGTLEDLDRLMKALHERDMKLVMDLVLNHTSDQHEWFKESRS 124
Query: 491 REPG-YENFFIWADPLPNPENPGVRLPPSNW 580
+ +++ W N + G RLPP+NW
Sbjct: 125 SKTNPKRDWYFWKPARYNEK--GERLPPNNW 153
>SPAC23D3.14c |aah2||alpha-amylase homolog Aah2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 581
Score = 52.0 bits (119), Expect = 6e-08
Identities = 27/73 (36%), Positives = 43/73 (58%), Gaps = 7/73 (9%)
Frame = +2
Query: 251 LKHFVDAGVDAIWMSPIFESPM---VD----FGYDISNFYDIHYEYGTMEDFEELLDKAH 409
L + G DAIW+SPIFE+ +D GY +N Y++++ +GT E+F EL+ + H
Sbjct: 75 LDYIQGMGFDAIWISPIFENVEGNDIDGSSYHGYWTTNLYELNHHFGTKEEFMELIQELH 134
Query: 410 ELGLKVLLDFVPN 448
+ + +LLD N
Sbjct: 135 KRDIWILLDVAIN 147
>SPCC63.02c |aah3||alpha-amylase homolog Aah3|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 564
Score = 44.4 bits (100), Expect = 1e-05
Identities = 25/75 (33%), Positives = 40/75 (53%), Gaps = 8/75 (10%)
Frame = +2
Query: 248 ELKHFVDAGVDAIWMSPI---FESPMVDFGYDISNFYDIHYE-----YGTMEDFEELLDK 403
+L + D G +AIW+SPI E + GY +++ YE +GT +D L+
Sbjct: 69 KLDYIEDMGFNAIWISPIDKNIEGDIDGAGYAYHGYWNTDYESLNEHFGTEDDLVSLITA 128
Query: 404 AHELGLKVLLDFVPN 448
AH+ G+ V+LD + N
Sbjct: 129 AHKAGIWVMLDSIVN 143
>SPCC11E10.09c ||SPCC188.01c|alpha-amylase homolog
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 478
Score = 41.1 bits (92), Expect = 1e-04
Identities = 24/74 (32%), Positives = 37/74 (50%), Gaps = 7/74 (9%)
Frame = +2
Query: 251 LKHFVDAGVDAIWMSPIFE--SPMVD-----FGYDISNFYDIHYEYGTMEDFEELLDKAH 409
L + G AIW+SPI + S D GY + ++ +GT ED +EL++ H
Sbjct: 60 LDYIKSLGCTAIWISPIVKNISETTDCGQAYHGYWAQDMTQLNENFGTEEDLKELVNAIH 119
Query: 410 ELGLKVLLDFVPNH 451
E + ++D V NH
Sbjct: 120 EKNMLCMVDIVVNH 133
>SPBC16A3.13 |meu7|aah4|alpha-amylase homolog
Aah4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 774
Score = 40.3 bits (90), Expect = 2e-04
Identities = 27/82 (32%), Positives = 40/82 (48%), Gaps = 7/82 (8%)
Frame = +2
Query: 251 LKHFVDAGVDAIWMSPI---FESPMVD----FGYDISNFYDIHYEYGTMEDFEELLDKAH 409
L + D G AI +SP+ E P G+ N+Y ++ +G ED +EL D H
Sbjct: 71 LDYIRDLGFTAISISPVVEQLEGPEYHGEAYHGHRPKNYYRLNPHFGNEEDLKELSDALH 130
Query: 410 ELGLKVLLDFVPNHASNESEYF 475
G+ +++D NH SEYF
Sbjct: 131 GKGMYLMVDVAINHTI--SEYF 150
>SPAC25H1.09 |mde5|meu30, SPAC4A8.01|alpha-amylase homolog
Mde5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 513
Score = 39.9 bits (89), Expect = 3e-04
Identities = 22/75 (29%), Positives = 38/75 (50%), Gaps = 7/75 (9%)
Frame = +2
Query: 248 ELKHFVDAGVDAIWMSPIFES-------PMVDFGYDISNFYDIHYEYGTMEDFEELLDKA 406
+L + G AIW+SPI ++ GY + Y ++ +GT +D +L D
Sbjct: 70 KLDYIQGMGFTAIWISPIIKNIEGRTKYGEAYHGYWPQDLYTLNPHFGTEQDLIDLADAL 129
Query: 407 HELGLKVLLDFVPNH 451
H+ G+ +++D V NH
Sbjct: 130 HDRGMYLMVDTVVNH 144
>SPAC27E2.01 |||alpha-amylase homolog |Schizosaccharomyces pombe|chr
1|||Manual
Length = 491
Score = 39.5 bits (88), Expect = 4e-04
Identities = 20/81 (24%), Positives = 40/81 (49%), Gaps = 7/81 (8%)
Frame = +2
Query: 248 ELKHFVDAGVDAIWMSPIFES-------PMVDFGYDISNFYDIHYEYGTMEDFEELLDKA 406
+L + G A+W+SPI ++ GY + ++ +GT +D EL+D+
Sbjct: 64 KLDYIQSLGCTAVWISPIVKNIEGVTGYGEAYHGYWAEDLTQLNPHFGTKQDLTELVDQL 123
Query: 407 HELGLKVLLDFVPNHASNESE 469
H+ + ++D V NH ++ +
Sbjct: 124 HKRNMLCMIDIVVNHMAHAGD 144
>SPBC32H8.13c |mok12||alpha-1,3-glucan synthase
Mok12|Schizosaccharomyces pombe|chr 2|||Manual
Length = 2352
Score = 37.1 bits (82), Expect = 0.002
Identities = 23/80 (28%), Positives = 38/80 (47%), Gaps = 2/80 (2%)
Frame = +2
Query: 251 LKHFVDAGVDAIWMS--PIFESPMVDFGYDISNFYDIHYEYGTMEDFEELLDKAHELGLK 424
L + G+ A++++ P P GY +F + GT+ + E + K HE G
Sbjct: 114 LDYLESMGIKAVYIAGTPFQNLPWYPDGYSPLDFTLLDKHTGTLNQWHEAIMKLHERGFY 173
Query: 425 VLLDFVPNHASNESEYFIKS 484
V++DF + S E YF+ S
Sbjct: 174 VVVDFTISTLS-ELSYFVNS 192
>SPCC757.12 |||alpha-amylase homolog |Schizosaccharomyces pombe|chr
3|||Manual
Length = 625
Score = 29.1 bits (62), Expect = 0.52
Identities = 16/75 (21%), Positives = 37/75 (49%), Gaps = 8/75 (10%)
Frame = +2
Query: 248 ELKHFVDAGVDAIWMSPIFESPMVDFGYD--------ISNFYDIHYEYGTMEDFEELLDK 403
+L + G +AIW+SP+ ++ +G D ++F ++ +G+ +D +L+
Sbjct: 69 KLDYIQGMGFNAIWISPVEKNLEGSYGSDGEAYHGYWNTDFTQLNEHFGSEDDLIDLITD 128
Query: 404 AHELGLKVLLDFVPN 448
H + ++ D + N
Sbjct: 129 MHNRDMWIMFDALAN 143
>SPBC354.07c |||oxysterol binding protein |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 399
Score = 26.6 bits (56), Expect = 2.7
Identities = 14/33 (42%), Positives = 19/33 (57%), Gaps = 1/33 (3%)
Frame = -2
Query: 355 YIVEVTDVVPKVDHGGFEY-RRHPNSIHACIYE 260
YIV T+ + K+D+ G Y R NS A I+E
Sbjct: 217 YIVSNTNYITKIDYSGRGYFRGTKNSFKATIFE 249
>SPBC106.04 |ada1||adenosine deaminase Ada1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 846
Score = 26.2 bits (55), Expect = 3.6
Identities = 13/43 (30%), Positives = 22/43 (51%)
Frame = +2
Query: 308 SPMVDFGYDISNFYDIHYEYGTMEDFEELLDKAHELGLKVLLD 436
SP V + + YD++ + G +E FEE++ E +V D
Sbjct: 481 SPNVRWLIQVPRLYDVYKKSGIVETFEEVVRNVFEPLFEVTKD 523
>SPBC119.17 ||SPBC577.01|metallopeptidase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 992
Score = 25.4 bits (53), Expect = 6.3
Identities = 11/25 (44%), Positives = 16/25 (64%)
Frame = +3
Query: 141 SYSWTGGRIAFFTKSTLDPLRTVMV 215
SYS G ++FFT DP+R++ V
Sbjct: 864 SYSGIDGVLSFFTYRDSDPIRSLSV 888
>SPAP27G11.03 |||D123 family|Schizosaccharomyces pombe|chr
1|||Manual
Length = 319
Score = 25.4 bits (53), Expect = 6.3
Identities = 15/50 (30%), Positives = 27/50 (54%), Gaps = 3/50 (6%)
Frame = +2
Query: 347 YDIHYEYGTMEDFEEL---LDKAHELGLKVLLDFVPNHASNESEYFIKSE 487
+D+++ + +DF+ + K L VL ++ P HAS E F+KS+
Sbjct: 134 HDLNHAFDDCKDFDNADGSVPKDFSFEL-VLKEWFPMHASTEFRCFVKSK 182
>SPAC23D3.06c |nup146||nucleoporin Nup146|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1325
Score = 25.4 bits (53), Expect = 6.3
Identities = 10/27 (37%), Positives = 16/27 (59%)
Frame = +2
Query: 401 KAHELGLKVLLDFVPNHASNESEYFIK 481
K HE+G+ +LD VP + + +F K
Sbjct: 949 KIHEVGVNKMLDVVPKEKRDLNSFFPK 975
>SPBC11B10.07c |||CDC50 domain protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 371
Score = 25.4 bits (53), Expect = 6.3
Identities = 13/33 (39%), Positives = 17/33 (51%)
Frame = +2
Query: 308 SPMVDFGYDISNFYDIHYEYGTMEDFEELLDKA 406
SP+ F Y + NFY H Y D +LL +A
Sbjct: 123 SPVFAF-YRLKNFYQNHRRYTVSADMFQLLGEA 154
>SPAC1834.08 |mak1|phk3|histidine kinase Mak1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1639
Score = 25.0 bits (52), Expect = 8.4
Identities = 13/23 (56%), Positives = 15/23 (65%)
Frame = +2
Query: 332 DISNFYDIHYEYGTMEDFEELLD 400
DIS+F DI T E FEELL+
Sbjct: 1363 DISSFKDIVRYGNTYEAFEELLE 1385
>SPAC31G5.05c |||ribulose phosphate 3-epimerase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 228
Score = 25.0 bits (52), Expect = 8.4
Identities = 11/30 (36%), Positives = 18/30 (60%)
Frame = +2
Query: 338 SNFYDIHYEYGTMEDFEELLDKAHELGLKV 427
++ + HYE E EE++ +AHE G+ V
Sbjct: 88 ASLFCFHYE--ATEKHEEIISRAHEKGMLV 115
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,436,120
Number of Sequences: 5004
Number of extensions: 48556
Number of successful extensions: 184
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 167
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 177
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 260219058
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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