Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fmgV14a07f
(644 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC186.08c |||L-lactate dehydrogenase |Schizosaccharomyces pomb... 41 2e-04
SPAC4H3.10c |pyk1||pyruvate kinase |Schizosaccharomyces pombe|ch... 36 0.005
SPCC1902.01 |gaf1|SPCC417.01c|transcription factor Gaf1 |Schizos... 28 1.3
SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyce... 28 1.3
SPAC105.03c |||transcription factor |Schizosaccharomyces pombe|c... 27 3.1
SPAC13A11.06 ||SPAC3H8.01|pyruvate decarboxylase |Schizosaccharo... 26 4.0
SPAC1F8.07c |||pyruvate decarboxylase |Schizosaccharomyces pombe... 26 5.3
SPAC31A2.05c |mis4||cohesin loading factor Mis4|Schizosaccharomy... 25 7.1
SPBC216.06c |swi1||replication fork protection complex subunit S... 25 9.3
>SPAC186.08c |||L-lactate dehydrogenase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 330
Score = 40.7 bits (91), Expect = 2e-04
Identities = 38/133 (28%), Positives = 53/133 (39%)
Frame = +2
Query: 227 EGVVMELADCALPLLAGVLPTANPEEAFKDVAAAFLVGAMPRKEGMERKDLLAANVRIFK 406
EG M+L A PL + KD A + +K G R DLL AN+ IFK
Sbjct: 59 EGEAMDLNHAA-PLSHETRVYLGDYKDCKDATAVVITAGKNQKPGETRMDLLKANISIFK 117
Query: 407 EQGQALDKVARKDVKVLVVGNPANTNALICSKYAPSIPKENFTAMTRLDQNRAQSQLAAK 586
E + + K KD +LV NP + K + + T +D R Q +
Sbjct: 118 EILREVTKYT-KDAILLVATNPVDVLTYATLKLTGFPAERVIGSGTIIDTARFQYLIGKL 176
Query: 587 IGVPVKDVKRVII 625
G+ + V II
Sbjct: 177 YGLDPQSVNADII 189
>SPAC4H3.10c |pyk1||pyruvate kinase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 509
Score = 35.9 bits (79), Expect = 0.005
Identities = 22/72 (30%), Positives = 33/72 (45%)
Frame = +2
Query: 419 ALDKVARKDVKVLVVGNPANTNALICSKYAPSIPKENFTAMTRLDQNRAQSQLAAKIGVP 598
A+ D K +VV + + A +CSKY PSIP +TR Q QS L +
Sbjct: 394 AIGASIESDAKAIVVLSTSGNTARLCSKYRPSIP---IVMVTRCPQRARQSHLNRGVYPV 450
Query: 599 VKDVKRVIIWGK 634
+ + + + W K
Sbjct: 451 IYEKEPLSDWQK 462
Score = 25.4 bits (53), Expect = 7.1
Identities = 17/58 (29%), Positives = 29/58 (50%)
Frame = +2
Query: 422 LDKVARKDVKVLVVGNPANTNALICSKYAPSIPKENFTAMTRLDQNRAQSQLAAKIGV 595
++KV K++KV V N N I SK ++PK + ++++A + K GV
Sbjct: 162 IEKVDDKNLKVRV-----NNNGKISSKKGVNLPKTDVDLPALSEKDKADLRFGVKNGV 214
>SPCC1902.01 |gaf1|SPCC417.01c|transcription factor Gaf1
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 855
Score = 27.9 bits (59), Expect = 1.3
Identities = 24/62 (38%), Positives = 31/62 (50%), Gaps = 2/62 (3%)
Frame = -3
Query: 360 PSFLGIAPTRKAAA--TSLKASSGFAVGRTPAKSGRAQSANSMTTPSSTPIIGAISRRWR 187
P F + P RK +A T+L SS P + RA SANS+T+P P A S + R
Sbjct: 516 PQFSAV-PHRKVSAQDTNLMGSSPGMYNHMPYLN-RATSANSITSPGVLPEGMAASLKKR 573
Query: 186 KT 181
T
Sbjct: 574 TT 575
>SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1036
Score = 27.9 bits (59), Expect = 1.3
Identities = 23/89 (25%), Positives = 37/89 (41%), Gaps = 5/89 (5%)
Frame = -3
Query: 465 PTTRTFTSLRATLSKAWPCSLKMRTLAARRSFLSIPSFLGIAPTRKAA-----ATSLKAS 301
P+ T + L + + P S T A S + S T ++ +T ++
Sbjct: 521 PSYNTSSVLPTSSVSSTPLSSANSTTATSASSTPLTSVNSTTATSASSTPFGNSTITSSA 580
Query: 300 SGFAVGRTPAKSGRAQSANSMTTPSSTPI 214
SG T SG + S+TTP+STP+
Sbjct: 581 SGSTGEFTNTNSGNGDVSGSVTTPTSTPL 609
>SPAC105.03c |||transcription factor |Schizosaccharomyces pombe|chr
1|||Manual
Length = 708
Score = 26.6 bits (56), Expect = 3.1
Identities = 11/30 (36%), Positives = 17/30 (56%)
Frame = -2
Query: 580 SKLGLSPVLIETSHGSEIFFWNRWSILRTY 491
+K LSP+L+ + S I +W+ LR Y
Sbjct: 337 AKHKLSPILLTAMYASSIIYWDADKELRNY 366
>SPAC13A11.06 ||SPAC3H8.01|pyruvate decarboxylase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 571
Score = 26.2 bits (55), Expect = 4.0
Identities = 8/22 (36%), Positives = 15/22 (68%)
Frame = -3
Query: 519 GIDGAYLEHIRAFVLAGLPTTR 454
G+ G+Y EH+ + G+P+T+
Sbjct: 87 GVAGSYAEHVPVVHIVGMPSTK 108
>SPAC1F8.07c |||pyruvate decarboxylase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 594
Score = 25.8 bits (54), Expect = 5.3
Identities = 10/26 (38%), Positives = 16/26 (61%)
Frame = -3
Query: 519 GIDGAYLEHIRAFVLAGLPTTRTFTS 442
GI GAY E++ +++G P T +S
Sbjct: 89 GIGGAYAENLPVILVSGSPNTNDLSS 114
>SPAC31A2.05c |mis4||cohesin loading factor Mis4|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1583
Score = 25.4 bits (53), Expect = 7.1
Identities = 14/41 (34%), Positives = 20/41 (48%)
Frame = -3
Query: 267 SGRAQSANSMTTPSSTPIIGAISRRWRKTGC*GPKTAPDAI 145
+ R+ S N TPSS+P + SR + PK + D I
Sbjct: 147 TNRSSSDNGFLTPSSSPRSPSCSRVFNAVQLCSPKKSKDDI 187
>SPBC216.06c |swi1||replication fork protection complex subunit
Swi1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 971
Score = 25.0 bits (52), Expect = 9.3
Identities = 16/47 (34%), Positives = 27/47 (57%)
Frame = -2
Query: 208 RNIKKVEEDRLLRSKNCSRRNLIKK*VCNLSGSTSYNNSDRFSHFDI 68
RNI +++E L++KN + + K + +L T +N D F HFD+
Sbjct: 174 RNILQIDE---LKTKNETIISFAKAHILDLI-VTLVSNLDEFEHFDV 216
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,677,972
Number of Sequences: 5004
Number of extensions: 55251
Number of successful extensions: 183
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 179
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 183
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 289756512
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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