BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fmgV13c16f
(585 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC1183.01 |sec15|SPCC1672.13|exocyst complex subunit Sec15 |Sc... 30 0.29
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 27 2.0
SPCC1840.04 |||caspase|Schizosaccharomyces pombe|chr 3|||Manual 27 2.7
SPAC2F7.10 |||palmitoyltransferase |Schizosaccharomyces pombe|ch... 26 3.5
SPAC10F6.09c |psm3|smc3|mitotic cohesin complex subunit Psm3|Sch... 26 4.7
SPCC364.06 |nap1||nucleosome assembly protein Nap1 |Schizosaccha... 25 6.2
SPAC11G7.02 |pub1||ubiquitin-protein ligase E3|Schizosaccharomyc... 25 8.1
SPAC10F6.01c ||SPAC4C5.05c|sulfite reductase beta subunit |Schiz... 25 8.1
SPBC2F12.13 |klp5|sot1|kinesin-like protein Klp5|Schizosaccharom... 25 8.1
>SPCC1183.01 |sec15|SPCC1672.13|exocyst complex subunit Sec15
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 785
Score = 29.9 bits (64), Expect = 0.29
Identities = 19/65 (29%), Positives = 33/65 (50%), Gaps = 2/65 (3%)
Frame = +3
Query: 153 SQEPSYVCL--HLILS*TMSTRAAGIFREEKTS*KFVAP*KVSTPPASAPSNWIASPTTY 326
S EP Y+CL H L S R++ R+ + +F+AP ++T + S W+ S +
Sbjct: 263 SFEPLYICLQVHSYLGLLSSFRSS-FERDRRRQQEFLAPKSLTTLDMAVVSEWLNSIAGF 321
Query: 327 SLVNF 341
+V +
Sbjct: 322 MIVEY 326
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 27.1 bits (57), Expect = 2.0
Identities = 17/58 (29%), Positives = 25/58 (43%)
Frame = -1
Query: 333 PVNKL*EMLSNSKVLKPAALTPSTEPRTFRMFSPPGRCQPPAYSLFTIGSNGDKRNWA 160
PVN L+ + +P+ P+ F+P + QPPA S S D NW+
Sbjct: 1594 PVNNSGSPLAMNAAGQPSLAVPAVPSAPSNHFNPFAKMQPPAPSPLQ-PSGHDSDNWS 1650
>SPCC1840.04 |||caspase|Schizosaccharomyces pombe|chr 3|||Manual
Length = 425
Score = 26.6 bits (56), Expect = 2.7
Identities = 12/28 (42%), Positives = 18/28 (64%)
Frame = -3
Query: 538 NRQLKFDVPDGVSVNTDSDNSTSANAHV 455
+RQ+KF D +S++ DN TSA+ V
Sbjct: 339 SRQVKFSPADVISLSGCKDNQTSADTSV 366
>SPAC2F7.10 |||palmitoyltransferase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 642
Score = 26.2 bits (55), Expect = 3.5
Identities = 12/29 (41%), Positives = 14/29 (48%)
Frame = -3
Query: 508 GVSVNTDSDNSTSANAHVGHGDSSRSVCG 422
G V NS+ N H HG R+VCG
Sbjct: 539 GTLVPPTKQNSSPKNGHGIHGSFLRTVCG 567
>SPAC10F6.09c |psm3|smc3|mitotic cohesin complex subunit
Psm3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1194
Score = 25.8 bits (54), Expect = 4.7
Identities = 22/79 (27%), Positives = 38/79 (48%), Gaps = 3/79 (3%)
Frame = +2
Query: 200 NEYAGGWHLPGGENILKVRGSVEGVNAAGFSTFE---LDSISYNLFTGQLHLSLSLNSVA 370
++YA H G + R +G AG+ + LD+I N+ T Q+ S +
Sbjct: 631 SQYARS-HQLNGITLSGDRSDKKGALTAGYRDYRNSRLDAIK-NVKTYQIKFS----DLQ 684
Query: 371 ASIDAAEGEVQIFDRKLTA 427
S++ E++ FD+K+TA
Sbjct: 685 ESLEKCRSEIESFDQKITA 703
>SPCC364.06 |nap1||nucleosome assembly protein Nap1
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 393
Score = 25.4 bits (53), Expect = 6.2
Identities = 17/58 (29%), Positives = 27/58 (46%), Gaps = 4/58 (6%)
Frame = -3
Query: 580 TPEQVQLDFRVDFSNRQLKFDVPDGVSVN----TDSDNSTSANAHVGHGDSSRSVCGQ 419
T E + L+ FS+ ++ D D S + +DSD S + H HG + + C Q
Sbjct: 335 TGEALALENYDGFSDLDVEEDEDDVESSSNEEVSDSDEEDSDSKHTAHGQQNAAECRQ 392
>SPAC11G7.02 |pub1||ubiquitin-protein ligase E3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 767
Score = 25.0 bits (52), Expect = 8.1
Identities = 12/27 (44%), Positives = 14/27 (51%)
Frame = -1
Query: 285 PAALTPSTEPRTFRMFSPPGRCQPPAY 205
PAA S+EPRTF F PP +
Sbjct: 185 PAASPASSEPRTFSSFEDQYGRLPPGW 211
>SPAC10F6.01c ||SPAC4C5.05c|sulfite reductase beta subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1473
Score = 25.0 bits (52), Expect = 8.1
Identities = 12/24 (50%), Positives = 15/24 (62%)
Frame = +2
Query: 77 VAAVPYGDVDLAESQRIVDGIIEN 148
VAA PY DVD+ + + IIEN
Sbjct: 1106 VAAPPYNDVDVYTNDVALIAIIEN 1129
>SPBC2F12.13 |klp5|sot1|kinesin-like protein
Klp5|Schizosaccharomyces pombe|chr 2|||Manual
Length = 883
Score = 25.0 bits (52), Expect = 8.1
Identities = 13/40 (32%), Positives = 22/40 (55%)
Frame = +2
Query: 83 AVPYGDVDLAESQRIVDGIIENWISRAQLRLSPFDPIVNN 202
A Y DV L + ++ IIEN + AQ ++ F P++ +
Sbjct: 556 ATMYADV-LQDEVDLLKSIIENQVLDAQNKVDEFTPVLES 594
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,009,113
Number of Sequences: 5004
Number of extensions: 36483
Number of successful extensions: 137
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 134
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 137
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 252150250
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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