Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fmgV13a02r
         (741 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC354.02c |sec61||translocon alpha subunit Sec61|Schizosacchar...   160   2e-40
SPBC19G7.17 ||SPBC36B7.01|translocon subunit Sec61 homolog |Schi...    87   3e-18
SPBC336.03 |efc25||exchange factor Cdc25p-like|Schizosaccharomyc...    29   0.92 
SPBC776.14 |plh1||phospholipid-diacylglycerol acyltransferase Pl...    28   1.2  
SPAC1486.06 |||nicotinate phosphoribosyltransferase |Schizosacch...    26   6.5  
SPCC622.10c |||exocyst complex subunit Sec5 |Schizosaccharomyces...    25   8.6  
SPAC17H9.13c |||glutamate 5-kinase |Schizosaccharomyces pombe|ch...    25   8.6  

>SPBC354.02c |sec61||translocon alpha subunit
           Sec61|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 479

 Score =  160 bits (389), Expect = 2e-40
 Identities = 81/155 (52%), Positives = 98/155 (63%)
 Frame = -1

Query: 738 SKFSGXFLVNVLGVWADXXXXXXXXXXXXXGLCYYLSPPESLAHIAHDPVHALLYVLFML 559
           ++FS  FLV  LGVW                L YYLSPP S      DP+H L+YV F +
Sbjct: 319 NRFSSNFLVRFLGVWEQTATSG---------LSYYLSPPASFQDALIDPIHTLVYVFFTM 369

Query: 558 GSCAFFSKTWIDVSGSSAKDVAKQLKEQQMVMRGHRDNSMIHELNRYIPTAAAFGGLCIG 379
            +CA FSK WI+VSG+S +DVAKQLK QQ+VM GHR+ SM  EL R IPTAA   G  +G
Sbjct: 370 FACALFSKLWIEVSGASPRDVAKQLKSQQLVMAGHREGSMYKELKRIIPTAAWLSGAVVG 429

Query: 378 ALSVLADFLGAIGSGTGILLAVTIIYQYFEIFVKE 274
           AL+V +D LGA+GSGT +LL  T IY Y+E   KE
Sbjct: 430 ALAVASDLLGALGSGTAVLLCTTTIYGYYEQLQKE 464


>SPBC19G7.17 ||SPBC36B7.01|translocon subunit Sec61 homolog
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 475

 Score = 86.6 bits (205), Expect = 3e-18
 Identities = 42/129 (32%), Positives = 69/129 (53%)
 Frame = -1

Query: 645 LCYYLSPPESLAHIAHDPVHALLYVLFMLGSCAFFSKTWIDVSGSSAKDVAKQLKEQQMV 466
           L Y+L PP  L+     PVH ++Y + ++    +FS  W++ +    +DV    KE Q+V
Sbjct: 345 LVYFLYPPLGLSEALLHPVHTVIYTITLICITIYFSLLWMNATAGGPRDVLLFFKENQLV 404

Query: 465 MRGHRDNSMIHELNRYIPTAAAFGGLCIGALSVLADFLGAIGSGTGILLAVTIIYQYFEI 286
           + G+R+ +M+ EL + IP AA      +  LSV+A    A   G G+L+A  ++Y  FE+
Sbjct: 405 IAGYREATMLKELEKIIPIAAKLSAFFVSILSVIAGIF-ASTFGVGVLIASALVYASFEM 463

Query: 285 FVKEQAEMG 259
            V     +G
Sbjct: 464 IVGANTSLG 472


>SPBC336.03 |efc25||exchange factor Cdc25p-like|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 987

 Score = 28.7 bits (61), Expect = 0.92
 Identities = 10/22 (45%), Positives = 14/22 (63%)
 Frame = -3

Query: 535 DLDRCVWLLSQRCGETTEGTAD 470
           +LD+CVW L + C E  E  +D
Sbjct: 378 ELDKCVWFLLKNCDEFIENFSD 399


>SPBC776.14 |plh1||phospholipid-diacylglycerol acyltransferase
           Plh1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 623

 Score = 28.3 bits (60), Expect = 1.2
 Identities = 18/65 (27%), Positives = 31/65 (47%), Gaps = 2/65 (3%)
 Frame = -1

Query: 429 LNRYIPTAAAFGGLCIGALSVLADFLGAIGSGTGILLAVTIIYQYFEIFVKEQ--AEMGG 256
           +N +I       G  IGA   +A  L      TGI++ + I+ ++F    +      MGG
Sbjct: 319 VNDHIEAFINISGSLIGAPKTVAALLSGEMKDTGIVITLNILEKFFSRSERAMMVRTMGG 378

Query: 255 MSTLL 241
           +S++L
Sbjct: 379 VSSML 383


>SPAC1486.06 |||nicotinate phosphoribosyltransferase
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 410

 Score = 25.8 bits (54), Expect = 6.5
 Identities = 8/21 (38%), Positives = 13/21 (61%)
 Frame = -1

Query: 210 DRCVQLRFYCRFCSESTSLGV 148
           DRC++L  YC  C   ++ G+
Sbjct: 326 DRCIELYKYCEKCGIKSAFGI 346


>SPCC622.10c |||exocyst complex subunit Sec5 |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 815

 Score = 25.4 bits (53), Expect = 8.6
 Identities = 13/33 (39%), Positives = 17/33 (51%)
 Frame = +2

Query: 341 PMAPRKSASTDRAPMHSPPNAAAVGM*RFSSWI 439
           PMA RK A T R    +     + G+ R SSW+
Sbjct: 102 PMATRKIAGTYRDAPDNSMQQTSTGVHRSSSWL 134


>SPAC17H9.13c |||glutamate 5-kinase |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 402

 Score = 25.4 bits (53), Expect = 8.6
 Identities = 13/43 (30%), Positives = 23/43 (53%), Gaps = 1/43 (2%)
 Frame = -1

Query: 543 FSKTWID-VSGSSAKDVAKQLKEQQMVMRGHRDNSMIHELNRY 418
           +S T ID + G  +K++A  L   +     +RD  ++H LN +
Sbjct: 350 YSSTEIDLIKGKRSKEIASILGYNETEYVAYRDYLVVHGLNSH 392


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,601,857
Number of Sequences: 5004
Number of extensions: 48944
Number of successful extensions: 142
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 136
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 140
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 351258950
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)