SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fmgV12k20r
         (716 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At4g10730.1 68417.m01753 protein kinase family protein contains ...    32   0.33 
At3g53470.2 68416.m05903 expressed protein ribosomal protein S25...    31   0.76 
At3g53470.1 68416.m05902 expressed protein ribosomal protein S25...    31   0.76 
At5g40740.1 68418.m04944 expressed protein                             29   3.1  
At3g30300.1 68416.m03826 expressed protein contains Pfam PF03138...    28   7.1  
At3g07400.1 68416.m00882 lipase class 3 family protein contains ...    28   7.1  
At3g03540.1 68416.m00355 phosphoesterase family protein similar ...    27   9.4  

>At4g10730.1 68417.m01753 protein kinase family protein contains
           protein kinase domain, Pfam:PF00069
          Length = 708

 Score = 32.3 bits (70), Expect = 0.33
 Identities = 25/96 (26%), Positives = 43/96 (44%), Gaps = 3/96 (3%)
 Frame = -1

Query: 626 INARQERLVTNQVASAIENIRKQIREA-GFD-PLDVDRREIVIPPEEDFHALAAFAEDIK 453
           IN+  E++   + + +   ++  +R A  F  PL++  R           +   F + I 
Sbjct: 483 INSDSEKVHGYERSESERQLKSSVRRAPSFSGPLNLPNRASANSLSAPIKSSGGFRDSID 542

Query: 452 STGLSNIVIITNNFSILSARLNLVLSLP-RVEASVG 348
               +N+V I   FS+ S  L+L  + P R  ASVG
Sbjct: 543 DKSKANVVQIKGRFSVTSENLDLARASPLRKSASVG 578


>At3g53470.2 68416.m05903 expressed protein ribosomal protein S25,
           cytosolic, Arabidopsis thaliana, PIR:T08568
          Length = 136

 Score = 31.1 bits (67), Expect = 0.76
 Identities = 19/63 (30%), Positives = 36/63 (57%), Gaps = 1/63 (1%)
 Frame = -1

Query: 581 AIENIRKQIREAGFDPLDVDRREIVIPPEEDFHALAAFAEDIKSTGLSNIVIITN-NFSI 405
           A +N +KQ +++ F   +VD+     PP  +F  L+   + IK T  SN+++ T+  F++
Sbjct: 58  AWKNFKKQSKKSLFSQFNVDKYVTWNPPRSEFD-LSEEVDPIKRTERSNLMLWTSPRFTL 116

Query: 404 LSA 396
           + A
Sbjct: 117 VGA 119


>At3g53470.1 68416.m05902 expressed protein ribosomal protein S25,
           cytosolic, Arabidopsis thaliana, PIR:T08568
          Length = 135

 Score = 31.1 bits (67), Expect = 0.76
 Identities = 19/63 (30%), Positives = 36/63 (57%), Gaps = 1/63 (1%)
 Frame = -1

Query: 581 AIENIRKQIREAGFDPLDVDRREIVIPPEEDFHALAAFAEDIKSTGLSNIVIITN-NFSI 405
           A +N +KQ +++ F   +VD+     PP  +F  L+   + IK T  SN+++ T+  F++
Sbjct: 57  AWKNFKKQSKKSLFSQFNVDKYVTWNPPRSEFD-LSEEVDPIKRTERSNLMLWTSPRFTL 115

Query: 404 LSA 396
           + A
Sbjct: 116 VGA 118


>At5g40740.1 68418.m04944 expressed protein
          Length = 741

 Score = 29.1 bits (62), Expect = 3.1
 Identities = 13/37 (35%), Positives = 22/37 (59%)
 Frame = -1

Query: 656 STQTFDEQTEINARQERLVTNQVASAIENIRKQIREA 546
           S +   +Q   NAR + L T+  +S + N++K +REA
Sbjct: 554 SEKNSTDQALSNARPDNLPTDTSSSFVHNLKKSVREA 590


>At3g30300.1 68416.m03826 expressed protein contains Pfam PF03138:
           Plant protein family. The function of this family of
           plant proteins is unknown; previously annotated as
           'auxin-independent growth promoter -related'  based on
           similarity to axi 1 protein (GB:X80301) (GI:559920) from
           [Nicotiana tabacum], which, due to scienitific fraud was
           retracted. Retraction in: Schell J. EMBO J 1999 May
           17;18(10):2908. PMID:10400497.
          Length = 677

 Score = 27.9 bits (59), Expect = 7.1
 Identities = 21/75 (28%), Positives = 35/75 (46%), Gaps = 1/75 (1%)
 Frame = -2

Query: 685 LFSPQFWLVPVHKLLMNRRR*THVKSAWSLTRLL-PPSKISESK*EKPDSIHWTLIEEKL 509
           +F  Q  L+P+H +  N    T + ++W L ++    +K ++ K   P SI    +E K 
Sbjct: 363 VFGGQRTLIPLHGMFENVVDRTSLSTSWELAKMYGREAKHNDIKKMTPPSIE---VETKH 419

Query: 508 SSLQRKTSMHLPLSP 464
            SL+       PL P
Sbjct: 420 DSLKSTRQRPQPLPP 434


>At3g07400.1 68416.m00882 lipase class 3 family protein contains
           Pfam profile PF01764: Lipase
          Length = 1003

 Score = 27.9 bits (59), Expect = 7.1
 Identities = 11/26 (42%), Positives = 18/26 (69%)
 Frame = -1

Query: 482 ALAAFAEDIKSTGLSNIVIITNNFSI 405
           ALA   +++KS G+  ++ ITN FS+
Sbjct: 818 ALALLLDEVKSLGIPWVLAITNKFSV 843


>At3g03540.1 68416.m00355 phosphoesterase family protein similar to
           SP|P95246 Phospholipase C 2 precursor (EC 3.1.4.3)
           {Mycobacterium tuberculosis}; contains Pfam profile
           PF04185: Phosphoesterase family
          Length = 521

 Score = 27.5 bits (58), Expect = 9.4
 Identities = 14/45 (31%), Positives = 25/45 (55%), Gaps = 1/45 (2%)
 Frame = +1

Query: 109 VCERKLVMVSERSRPNKLTFRSASTPPKVRSILRLLIE-IPETTL 240
           +C+R    V   ++PN+L   SA++     +  +LLIE  P+ T+
Sbjct: 145 ICDRWFASVPGATQPNRLFIHSATSHGTTNNERKLLIEGFPQKTI 189


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,281,651
Number of Sequences: 28952
Number of extensions: 284962
Number of successful extensions: 825
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 814
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 825
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1555552968
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -