Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fmgV12g07f
(556 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g14180.1 68418.m01658 lipase family protein similar to SP|Q64... 113 7e-26
At2g15230.1 68415.m01737 lipase family protein similar to SP|P07... 103 8e-23
At1g73920.2 68414.m08561 lipase family protein similar to lipase... 85 2e-17
At1g73920.1 68414.m08560 lipase family protein similar to lipase... 85 2e-17
At1g18460.1 68414.m02303 lipase family protein similar to triacy... 81 6e-16
At1g49580.1 68414.m05559 calcium-dependent protein kinase, putat... 37 0.008
At2g26720.1 68415.m03205 plastocyanin-like domain-containing pro... 30 0.90
At3g19100.1 68416.m02427 calcium-dependent protein kinase, putat... 30 1.2
At5g40210.1 68418.m04879 nodulin MtN21 family protein similar to... 29 1.6
At4g34570.1 68417.m04912 bifunctional dihydrofolate reductase-th... 29 1.6
At2g16370.1 68415.m01873 bifunctional dihydrofolate reductase-th... 29 1.6
At1g77670.1 68414.m09043 aminotransferase class I and II family ... 29 2.8
At1g65630.1 68414.m07444 DegP protease, putative contains simila... 27 6.4
At5g53640.1 68418.m06663 F-box family protein contains F-box dom... 27 8.4
At3g15290.1 68416.m01931 3-hydroxybutyryl-CoA dehydrogenase, put... 27 8.4
At1g32240.1 68414.m03966 myb family transcription factor (KAN2) ... 27 8.4
>At5g14180.1 68418.m01658 lipase family protein similar to SP|Q64194
Lysosomal acid lipase/cholesteryl ester hydrolase
precursor (EC 3.1.1.13) {Rattus norvegicus}; contains
Pfam profile PF04083: ab-hydrolase associated lipase
region
Length = 418
Score = 113 bits (272), Expect = 7e-26
Identities = 55/124 (44%), Positives = 74/124 (59%), Gaps = 1/124 (0%)
Frame = +2
Query: 188 YRYPVEVHKVVTEDGYILEMQRIPHGRDQNNRPDP-KKPAVLVMHGLFASAADWVLMGPG 364
+ Y E H VVT+DGYIL MQRIP GR D K+ VL+ HG+ W+L
Sbjct: 59 FGYKCEEHDVVTQDGYILNMQRIPEGRAGAVAGDGGKRQPVLIQHGILVDGMSWLLNPAD 118
Query: 365 VGLAYILAEAGYDVWLGNARGTYYSRAHIKLDPDNDSEFWKFSWEEIGTRDLPAMIDYTL 544
L ILA+ G+DVW+GN RGT +SR H L+P + FW ++W+E+ + DLPAM D+
Sbjct: 119 QNLPLILADQGFDVWMGNTRGTRFSRRHKYLNP-SQRAFWNWTWDELVSYDLPAMFDHIH 177
Query: 545 KVAG 556
+ G
Sbjct: 178 GLTG 181
>At2g15230.1 68415.m01737 lipase family protein similar to SP|P07098
Triacylglycerol lipase, gastric precursor (EC 3.1.1.3)
{Homo sapiens}; contains Pfam profile PF04083:
ab-hydrolase associated lipase region
Length = 393
Score = 103 bits (247), Expect = 8e-23
Identities = 51/126 (40%), Positives = 69/126 (54%)
Frame = +2
Query: 176 LIKKYRYPVEVHKVVTEDGYILEMQRIPHGRDQNNRPDPKKPAVLVMHGLFASAADWVLM 355
LI Y H + T+DGYIL +QR+ + P VL+ HGLF + W L
Sbjct: 36 LIHPANYSCTEHSIQTKDGYILALQRVASLGPRLQ----SGPPVLLQHGLFMAGDVWFLN 91
Query: 356 GPGVGLAYILAEAGYDVWLGNARGTYYSRAHIKLDPDNDSEFWKFSWEEIGTRDLPAMID 535
P L +ILA+ G+DVW+GN RGT YS H+ L D D EFW +SW+++ DL MI
Sbjct: 92 SPKESLGFILADHGFDVWVGNVRGTRYSYGHVTLS-DTDKEFWDWSWQDLAMYDLAEMIQ 150
Query: 536 YTLKVA 553
Y ++
Sbjct: 151 YLYSIS 156
>At1g73920.2 68414.m08561 lipase family protein similar to lipase
GB:CAA74737 [SP|O46108] from [Drosophila melanogaster]
Length = 635
Score = 85.4 bits (202), Expect = 2e-17
Identities = 49/120 (40%), Positives = 70/120 (58%)
Frame = +2
Query: 176 LIKKYRYPVEVHKVVTEDGYILEMQRIPHGRDQNNRPDPKKPAVLVMHGLFASAADWVLM 355
+I + YP E +V+T DGY+L ++RIP R D +K AV + HG+ S+ WV
Sbjct: 230 VITELGYPYEAIRVITSDGYVLVLERIP-------RRDARK-AVFLQHGVLDSSMGWVSN 281
Query: 356 GPGVGLAYILAEAGYDVWLGNARGTYYSRAHIKLDPDNDSEFWKFSWEEIGTRDLPAMID 535
G A+ + GYDV+LGN RG SR H+ + + EFW++S E GT D+PAMI+
Sbjct: 282 GVVGSPAFAAYDQGYDVFLGNFRG-LVSRDHVNKN-ISSKEFWRYSINEHGTEDIPAMIE 339
>At1g73920.1 68414.m08560 lipase family protein similar to lipase
GB:CAA74737 [SP|O46108] from [Drosophila melanogaster]
Length = 704
Score = 85.4 bits (202), Expect = 2e-17
Identities = 49/120 (40%), Positives = 70/120 (58%)
Frame = +2
Query: 176 LIKKYRYPVEVHKVVTEDGYILEMQRIPHGRDQNNRPDPKKPAVLVMHGLFASAADWVLM 355
+I + YP E +V+T DGY+L ++RIP R D +K AV + HG+ S+ WV
Sbjct: 299 VITELGYPYEAIRVITSDGYVLVLERIP-------RRDARK-AVFLQHGVLDSSMGWVSN 350
Query: 356 GPGVGLAYILAEAGYDVWLGNARGTYYSRAHIKLDPDNDSEFWKFSWEEIGTRDLPAMID 535
G A+ + GYDV+LGN RG SR H+ + + EFW++S E GT D+PAMI+
Sbjct: 351 GVVGSPAFAAYDQGYDVFLGNFRG-LVSRDHVNKN-ISSKEFWRYSINEHGTEDIPAMIE 408
>At1g18460.1 68414.m02303 lipase family protein similar to
triacylglycerol lipase, gastric precursor (EC 3.1.1.3)
{Canis familiaris} [SP|P80035]
Length = 701
Score = 80.6 bits (190), Expect = 6e-16
Identities = 49/120 (40%), Positives = 69/120 (57%)
Frame = +2
Query: 176 LIKKYRYPVEVHKVVTEDGYILEMQRIPHGRDQNNRPDPKKPAVLVMHGLFASAADWVLM 355
+I + YP E +VVT DGY L ++RIP R D +K AV + HG+ S+ WV
Sbjct: 297 VITELGYPYEAIRVVTSDGYGLLLERIP-------RRDARK-AVYLQHGVMDSSMGWVSN 348
Query: 356 GPGVGLAYILAEAGYDVWLGNARGTYYSRAHIKLDPDNDSEFWKFSWEEIGTRDLPAMID 535
G A+ + GYDV+LGN RG SR H+K + + +FW++S E T D+PAMI+
Sbjct: 349 GVVGSPAFAAYDQGYDVFLGNFRG-LVSRDHVKKN-ISSKDFWRYSINEHATEDIPAMIE 406
>At1g49580.1 68414.m05559 calcium-dependent protein kinase, putative
/ CDPK, putative similar to calcium/calmodulin-dependent
protein kinase CaMK3 [Nicotiana tabacum]
gi|16904226|gb|AAL30820; contains protein kinase domain,
Pfam:PF00069; contains serine/threonine protein kinase
domain, INTERPRO:IPR002290
Length = 606
Score = 37.1 bits (82), Expect = 0.008
Identities = 37/149 (24%), Positives = 65/149 (43%), Gaps = 17/149 (11%)
Frame = +2
Query: 95 EELYRLDAQGARYSTNVTEDALLDIVGLIKKYRYPVEVHKVVTEDGYIL-------EMQR 253
E L R+ A+G +YS N + ++ I+ ++ + VH+ + + ++ +++
Sbjct: 238 ELLDRILARGGKYSENDAKPVIIQILNVVAFCHFQGVVHRDLKPENFLYTSKEENSQLKA 297
Query: 254 IPHGRDQNNRPDPKKPAVL---------VMHGLFASAAD-WVLMGPGVGLAYILAEAGYD 403
I G RPD + ++ V+H + + AD W + GV +AYIL
Sbjct: 298 IDFGLSDFVRPDERLNDIVGSAYYVAPEVLHRSYTTEADVWSI---GV-IAYILLCGSRP 353
Query: 404 VWLGNARGTYYSRAHIKLDPDNDSEFWKF 490
W G + RA +K DP D W F
Sbjct: 354 FWARTESGIF--RAVLKADPSFDEPPWPF 380
>At2g26720.1 68415.m03205 plastocyanin-like domain-containing
protein / mavicyanin, putative similar to mavicyanin
SP:P80728 from [Cucurbita pepo]
Length = 206
Score = 30.3 bits (65), Expect = 0.90
Identities = 12/26 (46%), Positives = 18/26 (69%)
Frame = -2
Query: 396 PASAKMYAKPTPGPIKTQSAAEANSP 319
PAS A P PGP+++QS++ + SP
Sbjct: 127 PASLGHVAVPVPGPVRSQSSSSSPSP 152
>At3g19100.1 68416.m02427 calcium-dependent protein kinase, putative
/ CDPK, putative similar to calcium/calmodulin-dependent
protein kinase CaMK3 [Nicotiana tabacum]
gi|16904226|gb|AAL30820; contains protein kinase domain,
Pfam:PF00069; contains serine/threonine protein kinase
domain, INTERPRO:IPR002290
Length = 599
Score = 29.9 bits (64), Expect = 1.2
Identities = 36/147 (24%), Positives = 62/147 (42%), Gaps = 17/147 (11%)
Frame = +2
Query: 95 EELYRLDAQGARYSTNVTEDALLDIVGLIKKYRYPVEVHKVVTEDGYILE-------MQR 253
E L R+ A+G +YS + + L+ I+ ++ VH+ + + ++ ++
Sbjct: 232 ELLDRILARGGKYSEDDAKAVLIQILNVVAFCHLQGVVHRDLKPENFLYTSKEENSMLKV 291
Query: 254 IPHGRDQNNRPDPKKPAVL---------VMHGLFASAAD-WVLMGPGVGLAYILAEAGYD 403
I G RPD + ++ V+H + + AD W + GV +AYIL
Sbjct: 292 IDFGLSDFVRPDERLNDIVGSAYYVAPEVLHRSYTTEADVWSI---GV-IAYILLCGSRP 347
Query: 404 VWLGNARGTYYSRAHIKLDPDNDSEFW 484
W G + RA +K DP D W
Sbjct: 348 FWARTESGIF--RAVLKADPSFDEPPW 372
>At5g40210.1 68418.m04879 nodulin MtN21 family protein similar to
MtN21 GI:2598575 (root nodule development) from
[Medicago truncatula]
Length = 339
Score = 29.5 bits (63), Expect = 1.6
Identities = 18/53 (33%), Positives = 27/53 (50%), Gaps = 2/53 (3%)
Frame = +2
Query: 305 VLVMHG--LFASAADWVLMGPGVGLAYILAEAGYDVWLGNARGTYYSRAHIKL 457
V + HG L +S +DW++ G + L YIL Y V + + G Y S + L
Sbjct: 153 VTLYHGPMLMSSHSDWIIGGGLLALQYILVSVSYLV-MAHTMGRYPSAVVVTL 204
>At4g34570.1 68417.m04912 bifunctional dihydrofolate
reductase-thymidylate synthase 2 / DHFR-TS (THY-2)
identical to SP|Q05763
Length = 565
Score = 29.5 bits (63), Expect = 1.6
Identities = 16/57 (28%), Positives = 22/57 (38%), Gaps = 1/57 (1%)
Frame = +2
Query: 374 AYILAEAGYDVWLGNARGTYYSRAHIKLDPDND-SEFWKFSWEEIGTRDLPAMIDYT 541
A +L E G +W GNA Y + + D + F W G + DYT
Sbjct: 350 AKVLQEKGIRIWDGNASRAYLDGIGLTEREEGDLGPVYGFQWRHFGAKYTDMHADYT 406
>At2g16370.1 68415.m01873 bifunctional dihydrofolate
reductase-thymidylate synthase 1 / DHFR-TS (THY-1)
identical to GP:289193:L08593 [SP|Q05762]
Length = 519
Score = 29.5 bits (63), Expect = 1.6
Identities = 16/57 (28%), Positives = 22/57 (38%), Gaps = 1/57 (1%)
Frame = +2
Query: 374 AYILAEAGYDVWLGNARGTYYSRAHIKLDPDND-SEFWKFSWEEIGTRDLPAMIDYT 541
A +L E G +W GNA Y + + D + F W G + DYT
Sbjct: 304 AKVLQEKGIHIWDGNASREYLDGIGLTEREEGDLGPVYGFQWRHFGAKYTDMHADYT 360
>At1g77670.1 68414.m09043 aminotransferase class I and II family
protein similar to kynurenine aminotransferase
/glutamine transaminase K GI:1030066 [Rattus norvegicus]
Length = 440
Score = 28.7 bits (61), Expect = 2.8
Identities = 17/53 (32%), Positives = 27/53 (50%), Gaps = 1/53 (1%)
Frame = +2
Query: 383 LAEAGYDVWLGNARGTYYSRA-HIKLDPDNDSEFWKFSWEEIGTRDLPAMIDY 538
L E G+ V+ + GTY+ A H +ND F ++ EE+G +P + Y
Sbjct: 354 LKEVGFTVFPSS--GTYFVVADHTPFGMENDVAFCEYLIEEVGVVAIPTSVFY 404
>At1g65630.1 68414.m07444 DegP protease, putative contains
similarity to DegP2 protease GI:13172275 from
[Arabidopsis thaliana]
Length = 559
Score = 27.5 bits (58), Expect = 6.4
Identities = 13/34 (38%), Positives = 19/34 (55%)
Frame = +2
Query: 176 LIKKYRYPVEVHKVVTEDGYILEMQRIPHGRDQN 277
LI K +VHKV+ +D IL + +P G D +
Sbjct: 325 LINKINPLSDVHKVLKKDDIILAIDGVPIGNDSS 358
>At5g53640.1 68418.m06663 F-box family protein contains F-box domain
Pfam:PF00646
Length = 917
Score = 27.1 bits (57), Expect = 8.4
Identities = 11/19 (57%), Positives = 15/19 (78%)
Frame = -1
Query: 151 FSYIGRITGTLGVQPVQFL 95
FSY+ R++ TLGV +QFL
Sbjct: 384 FSYMSRLSVTLGVSDLQFL 402
>At3g15290.1 68416.m01931 3-hydroxybutyryl-CoA dehydrogenase,
putative similar to S(+)-beta-hydroxybutyryl CoA
dehydrogenase (3-hydroxybutyryl-CoA dehydrogenase)
[Paracoccus denitrificans] GI:12003356; contains Pfam
profiles PF02737: 3-hydroxyacyl-CoA dehydrogenase NAD
binding, PF00725: 3-hydroxyacyl-CoA dehydrogenase
C-terminal
Length = 294
Score = 27.1 bits (57), Expect = 8.4
Identities = 13/28 (46%), Positives = 17/28 (60%)
Frame = +2
Query: 362 GVGLAYILAEAGYDVWLGNARGTYYSRA 445
G G+A + A +G DVWL +A SRA
Sbjct: 16 GSGIAQLAATSGLDVWLMDADRDALSRA 43
>At1g32240.1 68414.m03966 myb family transcription factor (KAN2)
contains Pfam profile: PF00249 myb-like DNA-binding
domain; identical to cDNA GARP-like putative
transcription factor KANADI2 (KAN2) GI:15723594
Length = 388
Score = 27.1 bits (57), Expect = 8.4
Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 1/35 (2%)
Frame = -3
Query: 428 YHVHFQATHHNQLQPKCMLNQHLDPSK-PSQQQKQ 327
+H Q HH QL P C + L + P+QQQ+Q
Sbjct: 79 HHQQQQQHHHPQLLPNCNSSNILTSFQFPTQQQQQ 113
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,732,326
Number of Sequences: 28952
Number of extensions: 278006
Number of successful extensions: 761
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 731
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 751
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1053014392
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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