BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fmgV12g05f (538 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC1183.01 |sec15|SPCC1672.13|exocyst complex subunit Sec15 |Sc... 30 0.25 SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 27 1.8 SPAC10F6.09c |psm3|smc3|mitotic cohesin complex subunit Psm3|Sch... 26 4.1 SPAC11G7.02 |pub1||ubiquitin-protein ligase E3|Schizosaccharomyc... 25 7.2 SPAC10F6.01c ||SPAC4C5.05c|sulfite reductase beta subunit |Schiz... 25 7.2 SPBC2F12.13 |klp5|sot1|kinesin-like protein Klp5|Schizosaccharom... 25 7.2 >SPCC1183.01 |sec15|SPCC1672.13|exocyst complex subunit Sec15 |Schizosaccharomyces pombe|chr 3|||Manual Length = 785 Score = 29.9 bits (64), Expect = 0.25 Identities = 19/65 (29%), Positives = 33/65 (50%), Gaps = 2/65 (3%) Frame = +1 Query: 151 SQEPSYVCL--HLILS*TMSTRAAGIFREEKTS*KFVAP*KVSTPPASAPSNWIASPTTY 324 S EP Y+CL H L S R++ R+ + +F+AP ++T + S W+ S + Sbjct: 263 SFEPLYICLQVHSYLGLLSSFRSS-FERDRRRQQEFLAPKSLTTLDMAVVSEWLNSIAGF 321 Query: 325 SLVNF 339 +V + Sbjct: 322 MIVEY 326 >SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF hand and WH2 motif |Schizosaccharomyces pombe|chr 1|||Manual Length = 1794 Score = 27.1 bits (57), Expect = 1.8 Identities = 17/58 (29%), Positives = 25/58 (43%) Frame = -1 Query: 331 PVNKL*EMLSNSKVLKPAALTPSTEPRTFRMFSPPGRCQPPAYSLFTIGSNGDKRNWA 158 PVN L+ + +P+ P+ F+P + QPPA S S D NW+ Sbjct: 1594 PVNNSGSPLAMNAAGQPSLAVPAVPSAPSNHFNPFAKMQPPAPSPLQ-PSGHDSDNWS 1650 >SPAC10F6.09c |psm3|smc3|mitotic cohesin complex subunit Psm3|Schizosaccharomyces pombe|chr 1|||Manual Length = 1194 Score = 25.8 bits (54), Expect = 4.1 Identities = 22/79 (27%), Positives = 38/79 (48%), Gaps = 3/79 (3%) Frame = +3 Query: 198 NEYAGGWHLPGGENILKVRGSVEGVNAAGFSTFE---LDSISYNLFTGQLHLSLSLNSVA 368 ++YA H G + R +G AG+ + LD+I N+ T Q+ S + Sbjct: 631 SQYARS-HQLNGITLSGDRSDKKGALTAGYRDYRNSRLDAIK-NVKTYQIKFS----DLQ 684 Query: 369 ASIDAAEGEVQIFDRKLTA 425 S++ E++ FD+K+TA Sbjct: 685 ESLEKCRSEIESFDQKITA 703 >SPAC11G7.02 |pub1||ubiquitin-protein ligase E3|Schizosaccharomyces pombe|chr 1|||Manual Length = 767 Score = 25.0 bits (52), Expect = 7.2 Identities = 12/27 (44%), Positives = 14/27 (51%) Frame = -1 Query: 283 PAALTPSTEPRTFRMFSPPGRCQPPAY 203 PAA S+EPRTF F PP + Sbjct: 185 PAASPASSEPRTFSSFEDQYGRLPPGW 211 >SPAC10F6.01c ||SPAC4C5.05c|sulfite reductase beta subunit |Schizosaccharomyces pombe|chr 1|||Manual Length = 1473 Score = 25.0 bits (52), Expect = 7.2 Identities = 12/24 (50%), Positives = 15/24 (62%) Frame = +3 Query: 75 VAAVPYGDVDLAESQRIVDGIIEN 146 VAA PY DVD+ + + IIEN Sbjct: 1106 VAAPPYNDVDVYTNDVALIAIIEN 1129 >SPBC2F12.13 |klp5|sot1|kinesin-like protein Klp5|Schizosaccharomyces pombe|chr 2|||Manual Length = 883 Score = 25.0 bits (52), Expect = 7.2 Identities = 13/40 (32%), Positives = 22/40 (55%) Frame = +3 Query: 81 AVPYGDVDLAESQRIVDGIIENWISRAQLRLSPFDPIVNN 200 A Y DV L + ++ IIEN + AQ ++ F P++ + Sbjct: 556 ATMYADV-LQDEVDLLKSIIENQVLDAQNKVDEFTPVLES 594 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,872,028 Number of Sequences: 5004 Number of extensions: 33938 Number of successful extensions: 116 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 113 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 116 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 222442660 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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