Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fmgV12f23f
(607 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g45140.1 68415.m05618 vesicle-associated membrane protein, pu... 64 9e-11
At3g60600.1 68416.m06781 vesicle-associated membrane protein, pu... 63 1e-10
At1g08820.1 68414.m00982 vesicle-associated membrane family prot... 60 1e-09
At5g47180.2 68418.m05818 vesicle-associated membrane family prot... 56 2e-08
At5g47180.1 68418.m05817 vesicle-associated membrane family prot... 56 2e-08
At4g00170.1 68417.m00018 vesicle-associated membrane family prot... 52 3e-07
At2g23830.1 68415.m02847 vesicle-associated membrane protein, pu... 52 4e-07
At1g51270.1 68414.m05766 vesicle-associated membrane protein, pu... 50 1e-06
At5g54110.1 68418.m06737 vesicle-associated membrane family prot... 36 0.021
At4g21450.2 68417.m03102 vesicle-associated membrane family prot... 34 0.084
At4g21450.1 68417.m03103 vesicle-associated membrane family prot... 34 0.084
At4g05060.1 68417.m00746 vesicle-associated membrane family prot... 33 0.19
At5g09320.1 68418.m01080 vacuolar sorting protein 9 domain-conta... 28 5.5
>At2g45140.1 68415.m05618 vesicle-associated membrane protein,
putative / VAMP, putative similar to VAP27 GI:6688926
[Nicotiana plumbaginifolia]
Length = 239
Score = 63.7 bits (148), Expect = 9e-11
Identities = 33/73 (45%), Positives = 47/73 (64%)
Frame = +2
Query: 389 MPNQVLTIEPQNELKFKVNFSGLFEHGCTTYMRLTNPTNDTVLFKIKTTAPKKYCVRPNS 568
M N++LTI+P +L+F + C+ Y L N T++ V FK+KTT PKKYCVRPN+
Sbjct: 1 MSNELLTIDPV-DLQFPFELKK--QISCSLY--LGNKTDNYVAFKVKTTNPKKYCVRPNT 55
Query: 569 GVLAPNSKQEIAI 607
GV+ P S E+ +
Sbjct: 56 GVVHPRSSSEVLV 68
>At3g60600.1 68416.m06781 vesicle-associated membrane protein,
putative / VAMP, putative similar to VAP27 GI:6688926
[Nicotiana plumbaginifolia]
Length = 256
Score = 63.3 bits (147), Expect = 1e-10
Identities = 32/71 (45%), Positives = 47/71 (66%)
Frame = +2
Query: 395 NQVLTIEPQNELKFKVNFSGLFEHGCTTYMRLTNPTNDTVLFKIKTTAPKKYCVRPNSGV 574
+++LT+EP +L+F + C+ Y LTN T++ V FK+KTT PKKYCVRPN+GV
Sbjct: 20 SELLTVEPL-DLQFPFELKK--QISCSLY--LTNKTDNNVAFKVKTTNPKKYCVRPNTGV 74
Query: 575 LAPNSKQEIAI 607
+ P S E+ +
Sbjct: 75 VLPRSTCEVLV 85
>At1g08820.1 68414.m00982 vesicle-associated membrane family protein
/ VAMP family protein similar to VAP27 GI:6688926
[Nicotiana plumbaginifolia]
Length = 386
Score = 59.7 bits (138), Expect = 1e-09
Identities = 32/69 (46%), Positives = 45/69 (65%)
Frame = +2
Query: 401 VLTIEPQNELKFKVNFSGLFEHGCTTYMRLTNPTNDTVLFKIKTTAPKKYCVRPNSGVLA 580
+L I+P+ L+F V+ + C ++LTN T+ V FK+KTT+PKKYCVRPN GV+A
Sbjct: 5 LLDIQPRT-LQFAVDLKK--QTSCV--VQLTNTTHHYVAFKVKTTSPKKYCVRPNVGVVA 59
Query: 581 PNSKQEIAI 607
P S E +
Sbjct: 60 PKSTCEFTV 68
>At5g47180.2 68418.m05818 vesicle-associated membrane family protein
/ VAMP family protein similar to VAP27 GI:6688926
[Nicotiana plumbaginifolia], to VAMP-associated protein
B GI:4240464 [Rattus norvegicus] and to
Vesicle-associated membrane protein/synaptobrevin
binding protein (VAP-33) (SP:Q16943)[Aplysia
californica]
Length = 220
Score = 55.6 bits (128), Expect = 2e-08
Identities = 31/65 (47%), Positives = 45/65 (69%), Gaps = 2/65 (3%)
Frame = +2
Query: 395 NQVLTIEPQNELKFKVNFSGLFEHGCTTY--MRLTNPTNDTVLFKIKTTAPKKYCVRPNS 568
NQ+++I+P +ELKF LFE +Y +++ N T + V FK+KTT+PKKY VRPN+
Sbjct: 7 NQLISIQP-DELKF------LFELEKQSYCDLKVANKTENYVAFKVKTTSPKKYFVRPNT 59
Query: 569 GVLAP 583
GV+ P
Sbjct: 60 GVIQP 64
>At5g47180.1 68418.m05817 vesicle-associated membrane family protein
/ VAMP family protein similar to VAP27 GI:6688926
[Nicotiana plumbaginifolia], to VAMP-associated protein
B GI:4240464 [Rattus norvegicus] and to
Vesicle-associated membrane protein/synaptobrevin
binding protein (VAP-33) (SP:Q16943)[Aplysia
californica]
Length = 220
Score = 55.6 bits (128), Expect = 2e-08
Identities = 31/65 (47%), Positives = 45/65 (69%), Gaps = 2/65 (3%)
Frame = +2
Query: 395 NQVLTIEPQNELKFKVNFSGLFEHGCTTY--MRLTNPTNDTVLFKIKTTAPKKYCVRPNS 568
NQ+++I+P +ELKF LFE +Y +++ N T + V FK+KTT+PKKY VRPN+
Sbjct: 7 NQLISIQP-DELKF------LFELEKQSYCDLKVANKTENYVAFKVKTTSPKKYFVRPNT 59
Query: 569 GVLAP 583
GV+ P
Sbjct: 60 GVIQP 64
>At4g00170.1 68417.m00018 vesicle-associated membrane family protein
/ VAMP family protein similar to VAP27 GI:6688926
[Nicotiana plumbaginifolia]
Length = 239
Score = 52.0 bits (119), Expect = 3e-07
Identities = 29/70 (41%), Positives = 40/70 (57%), Gaps = 1/70 (1%)
Frame = +2
Query: 401 VLTIEPQNELKFKVNFSGLFEHGCTTYMRLTNPTN-DTVLFKIKTTAPKKYCVRPNSGVL 577
++ I P ELKF + C+ M+LTN T V FK+KTT P+KYCVRPN+GV+
Sbjct: 6 LVNIHP-TELKFPFELKK--QSSCS--MQLTNKTTTQCVAFKVKTTNPRKYCVRPNTGVV 60
Query: 578 APNSKQEIAI 607
P + +
Sbjct: 61 LPGDSCNVTV 70
>At2g23830.1 68415.m02847 vesicle-associated membrane protein,
putative / VAMP, putative similar to VAP27 GI:6688926
[Nicotiana plumbaginifolia]
Length = 149
Score = 51.6 bits (118), Expect = 4e-07
Identities = 28/71 (39%), Positives = 41/71 (57%)
Frame = +2
Query: 395 NQVLTIEPQNELKFKVNFSGLFEHGCTTYMRLTNPTNDTVLFKIKTTAPKKYCVRPNSGV 574
N++L IEP L+F + C+ Y LTN T++ V FK+KTT YCVRPN G+
Sbjct: 4 NELLEIEPMY-LQFPFELKK--QMSCSLY--LTNKTDNNVAFKVKTTNRNNYCVRPNYGL 58
Query: 575 LAPNSKQEIAI 607
+ P S ++ +
Sbjct: 59 ILPKSTCKVLV 69
>At1g51270.1 68414.m05766 vesicle-associated membrane protein,
putative / VAMP, putative similar to VAP27 GI:6688926
[Nicotiana plumbaginifolia]
Length = 289
Score = 50.0 bits (114), Expect = 1e-06
Identities = 28/74 (37%), Positives = 45/74 (60%)
Frame = +2
Query: 386 KMPNQVLTIEPQNELKFKVNFSGLFEHGCTTYMRLTNPTNDTVLFKIKTTAPKKYCVRPN 565
+M +++L I+P +++F + + + C+ + LTN T + V FK KTT KKY VRPN
Sbjct: 123 RMSDELLIIDPV-DVQFPIELNK--KVSCS--LNLTNKTENYVAFKAKTTNAKKYYVRPN 177
Query: 566 SGVLAPNSKQEIAI 607
GV+ P S E+ +
Sbjct: 178 VGVVLPRSSCEVLV 191
>At5g54110.1 68418.m06737 vesicle-associated membrane family protein
/ VAMP family protein similar to VAP27 GI:6688926
[Nicotiana plumbaginifolia]
Length = 266
Score = 35.9 bits (79), Expect = 0.021
Identities = 16/34 (47%), Positives = 21/34 (61%)
Frame = +2
Query: 482 MRLTNPTNDTVLFKIKTTAPKKYCVRPNSGVLAP 583
++L N + FK +TTAPK +RP GVLAP
Sbjct: 99 IKLKNTSKSHTAFKFQTTAPKSCYMRPPGGVLAP 132
>At4g21450.2 68417.m03102 vesicle-associated membrane family protein
/ VAMP family protein similar to VAP27 GI:6688926
[Nicotiana plumbaginifolia]
Length = 212
Score = 33.9 bits (74), Expect = 0.084
Identities = 14/34 (41%), Positives = 21/34 (61%)
Frame = +2
Query: 482 MRLTNPTNDTVLFKIKTTAPKKYCVRPNSGVLAP 583
+++ N + V FK +TTAPK +RP +LAP
Sbjct: 130 IKIKNTSKSHVAFKFQTTAPKSCFMRPPGAILAP 163
>At4g21450.1 68417.m03103 vesicle-associated membrane family protein
/ VAMP family protein similar to VAP27 GI:6688926
[Nicotiana plumbaginifolia]
Length = 295
Score = 33.9 bits (74), Expect = 0.084
Identities = 14/34 (41%), Positives = 21/34 (61%)
Frame = +2
Query: 482 MRLTNPTNDTVLFKIKTTAPKKYCVRPNSGVLAP 583
+++ N + V FK +TTAPK +RP +LAP
Sbjct: 130 IKIKNTSKSHVAFKFQTTAPKSCFMRPPGAILAP 163
>At4g05060.1 68417.m00746 vesicle-associated membrane family protein
/ VAMP family protein similar to VAP27 GI:6688926
[Nicotiana plumbaginifolia]
Length = 287
Score = 32.7 bits (71), Expect = 0.19
Identities = 13/37 (35%), Positives = 21/37 (56%)
Frame = +2
Query: 482 MRLTNPTNDTVLFKIKTTAPKKYCVRPNSGVLAPNSK 592
+++ N + V FK +TT PK +RP +LAP +
Sbjct: 122 IKIKNTSKSHVAFKFQTTVPKSCFMRPAGAILAPGEE 158
>At5g09320.1 68418.m01080 vacuolar sorting protein 9
domain-containing protein / VPS9 domain-containing
protein contains similarity to Rab5 GDP/GTP exchange
factor, Rabex5 [Bos taurus] gi|2558516|emb|CAA04545;
contains Pfam profile PF02204: Vacuolar sorting protein
9 (VPS9) domain
Length = 712
Score = 27.9 bits (59), Expect = 5.5
Identities = 13/41 (31%), Positives = 19/41 (46%)
Frame = +1
Query: 478 LHETNEPDKRYSAIQDQNNSTKEILRASQFWSSCAKFETRN 600
L+ +P+K A+QD + RA WS C+ E N
Sbjct: 34 LNTAPDPEKDCDAVQDFFYKMESAFRAHPLWSGCSDDELDN 74
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,627,773
Number of Sequences: 28952
Number of extensions: 251438
Number of successful extensions: 606
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 593
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 606
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1206913392
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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