BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fmgV12f16r (749 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g15780.1 68418.m01845 pollen Ole e 1 allergen and extensin fa... 35 0.066 At4g18060.1 68417.m02687 SH3 domain-containing protein 3 (SH3P3)... 29 4.4 At2g25010.1 68415.m02990 expressed protein 29 4.4 At3g29390.1 68416.m03693 hydroxyproline-rich glycoprotein family... 28 7.6 At2g18510.1 68415.m02157 pre-mRNA splicing factor, putative simi... 28 7.6 At1g15110.1 68414.m01804 phosphatidyl serine synthase family pro... 28 7.6 >At5g15780.1 68418.m01845 pollen Ole e 1 allergen and extensin family protein contains Pfam profile PF01190: Pollen proteins Ole e I family Length = 401 Score = 34.7 bits (76), Expect = 0.066 Identities = 19/51 (37%), Positives = 26/51 (50%), Gaps = 2/51 (3%) Frame = +1 Query: 70 PSLATNELTPMSHSFPWWLIVNGPPESPR--QIPTPPTPLVQMLESKTTLP 216 P+L N L P S P ++ PP P +PTPPTP + + + TLP Sbjct: 284 PTLPPNPLIPSPPSLPPIPLIPTPPTLPTIPLLPTPPTPTLPPIPTIPTLP 334 >At4g18060.1 68417.m02687 SH3 domain-containing protein 3 (SH3P3) nearly identical to SH3 domain-containing protein 3 [Arabidopsis thaliana] GI:16974680; contains Pfam profile PF00018: SH3 domain Length = 351 Score = 28.7 bits (61), Expect = 4.4 Identities = 12/33 (36%), Positives = 20/33 (60%) Frame = -1 Query: 746 QAEQHQIFMQRIVGGAIAPINYHPYLAGLLIDI 648 +++QH++ QR+V NYH +A +L DI Sbjct: 222 ESQQHRLTFQRLVAMVEGEKNYHLRIAAILSDI 254 >At2g25010.1 68415.m02990 expressed protein Length = 509 Score = 28.7 bits (61), Expect = 4.4 Identities = 12/35 (34%), Positives = 19/35 (54%) Frame = -3 Query: 630 GRVRRIDPYPCQHLNCSSLLVRWKKSCSTFHCRTG 526 G R+I P + S+L+ RW++ +TFH G Sbjct: 61 GYFRKIGPMSLNNSLISALVERWRRETNTFHLPLG 95 >At3g29390.1 68416.m03693 hydroxyproline-rich glycoprotein family protein sequencing discrepancy between cDNA and genomic sequence prevents representation of entire coding sequence Length = 578 Score = 27.9 bits (59), Expect = 7.6 Identities = 14/43 (32%), Positives = 21/43 (48%) Frame = +1 Query: 139 PPESPRQIPTPPTPLVQMLESKTTLP**PRQVSKDIVCRKTFL 267 PP P+ I PP+ + SK+ LP PR + + K+ L Sbjct: 480 PPPPPKTIAPPPSKTMSPPSSKSMLPPPPRSKTMSPLSSKSML 522 >At2g18510.1 68415.m02157 pre-mRNA splicing factor, putative similar to SP|Q15427 Splicing factor 3B subunit 4 (Spliceosome associated protein 49) (SAP 49) (SF3b50) (Pre-mRNA splicing factor SF3b 49 kDa subunit) {Homo sapiens}; contains Pfam profile PF00076: RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain) Length = 363 Score = 27.9 bits (59), Expect = 7.6 Identities = 22/71 (30%), Positives = 27/71 (38%) Frame = +1 Query: 19 ALKEGTLAKTDGKPNSQPSLATNELTPMSHSFPWWLIVNGPPESPRQIPTPPTPLVQMLE 198 A K+ T + G P + ATN S P L GPP S Q+ P P Sbjct: 189 AYKKDTKGERHGTPAERLLAATNPTAQKSR--PHTLFAMGPPSSAPQVNGLPRPFANGSM 246 Query: 199 SKTTLP**PRQ 231 +P PRQ Sbjct: 247 QPVPIP-APRQ 256 >At1g15110.1 68414.m01804 phosphatidyl serine synthase family protein contains Pfam profile: PF03034 phosphatidyl serine synthase Length = 425 Score = 27.9 bits (59), Expect = 7.6 Identities = 13/38 (34%), Positives = 23/38 (60%) Frame = -1 Query: 392 AVQPIPLATDSLLSTDKAGMWAVAAGYGRYSDVINPTT 279 A+ P +D L+++ K G+WA+ A + YS + P+T Sbjct: 59 ALDPDSTTSDDLVTSVKRGVWAMIAVFLAYSLLQAPST 96 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 18,203,297 Number of Sequences: 28952 Number of extensions: 420258 Number of successful extensions: 1301 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 1132 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1296 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1663169840 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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