SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fmgV12e24f
         (559 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g69730.1 68414.m08024 protein kinase family protein contains ...    32   0.30 
At1g54450.1 68414.m06211 calcium-binding EF-hand family protein ...    30   1.2  
At1g69790.1 68414.m08030 protein kinase, putative similar to pro...    29   2.8  
At5g63540.1 68418.m07975 expressed protein  ; expression support...    28   4.9  
At4g14310.2 68417.m02205 peroxisomal membrane protein-related co...    27   6.4  
At4g14310.1 68417.m02204 peroxisomal membrane protein-related co...    27   6.4  
At3g19380.1 68416.m02458 U-box domain-containing protein contain...    27   6.4  
At2g30250.1 68415.m03682 WRKY family transcription factor              27   6.4  

>At1g69730.1 68414.m08024 protein kinase family protein contains
           Pfam profile: PF00069 Eukaryotic protein kinase domain
          Length = 792

 Score = 31.9 bits (69), Expect = 0.30
 Identities = 18/58 (31%), Positives = 27/58 (46%)
 Frame = +3

Query: 264 PVYIHRGNYLSPTGNTGQIRLNRGEQVLIACTGSGRTIRHPNVASNLAVGTVSCQNNN 437
           P Y+ + N L   G      L   +  ++ CT S  T  H ++   LAV  ++CQN N
Sbjct: 128 PFYVSQHNELVAVGCNNTASLTNVKPSIVQCTSSCSTKPHTHIKDYLAV--LNCQNYN 183


>At1g54450.1 68414.m06211 calcium-binding EF-hand family protein
           contains Pfam profile: PF00036 EF hand
          Length = 535

 Score = 29.9 bits (64), Expect = 1.2
 Identities = 14/48 (29%), Positives = 23/48 (47%)
 Frame = +3

Query: 96  LPEPGQLAFVLNEDEFEDYLDAYLALQQSEMLANQTRNDFRSGCTFRV 239
           LPE   ++   N+D  +   D +LAL ++  L     ND ++G    V
Sbjct: 13  LPETSPMSMKSNQDFVKKLFDQWLALPETNRLVTSLVNDAKAGVALNV 60


>At1g69790.1 68414.m08030 protein kinase, putative similar to
           protein kinase APK1A [Arabidopsis thaliana]
           SWISS-PROT:Q06548
          Length = 387

 Score = 28.7 bits (61), Expect = 2.8
 Identities = 14/33 (42%), Positives = 20/33 (60%), Gaps = 1/33 (3%)
 Frame = +3

Query: 360 GSGRTIRHPNVASNLAVGTV-SCQNNNLVTANW 455
           GS R  R PN +S L+  T+ S  NN+  T++W
Sbjct: 20  GSSRISRKPNQSSRLSSLTIPSYSNNSFTTSSW 52


>At5g63540.1 68418.m07975 expressed protein  ; expression supported
           by MPSS
          Length = 602

 Score = 27.9 bits (59), Expect = 4.9
 Identities = 23/92 (25%), Positives = 42/92 (45%), Gaps = 5/92 (5%)
 Frame = +3

Query: 270 YIH-RGNYL--SPTGNTGQIRLNRGEQVLIACT-GSGRTIRHPNVASNLAVGTVSCQNNN 437
           ++H  GN++  S +GN    + N+G  V +  T  S R +  P  ++N+       ++  
Sbjct: 270 HVHDTGNHVNSSASGNASHTQANQGIPVHVTRTHNSSRAMDEPLASTNVGATVSRVEHMQ 329

Query: 438 LVTANWLRGNSAFGQL-TCSSHAHHDAQQTNT 530
           + TA+   G   F  + + SS+ H  A    T
Sbjct: 330 IDTAS-AHGERTFSDIHSTSSNIHRAASTAGT 360


>At4g14310.2 68417.m02205 peroxisomal membrane protein-related
            contains weak similarity to Peroxisomal membrane protein
            2 (22 kDa peroxisomal membrane protein)
            (Swiss-Prot:P42925) [Mus musculus]
          Length = 965

 Score = 27.5 bits (58), Expect = 6.4
 Identities = 21/78 (26%), Positives = 32/78 (41%)
 Frame = +3

Query: 282  GNYLSPTGNTGQIRLNRGEQVLIACTGSGRTIRHPNVASNLAVGTVSCQNNNLVTANWLR 461
            G  +   G   Q   +RG+ V + CT    T++   +AS+  V   S +   LV      
Sbjct: 816  GAKIPKLGVNAQCVSSRGDSVFLGCTNQKSTVK-KQMASSSQVQQFSIRKQRLV------ 868

Query: 462  GNSAFGQLTCSSHAHHDA 515
              S +     +SH HH A
Sbjct: 869  --STYSLPDSNSHPHHSA 884


>At4g14310.1 68417.m02204 peroxisomal membrane protein-related
           contains weak similarity to Peroxisomal membrane protein
           2 (22 kDa peroxisomal membrane protein)
           (Swiss-Prot:P42925) [Mus musculus]
          Length = 1087

 Score = 27.5 bits (58), Expect = 6.4
 Identities = 21/78 (26%), Positives = 32/78 (41%)
 Frame = +3

Query: 282 GNYLSPTGNTGQIRLNRGEQVLIACTGSGRTIRHPNVASNLAVGTVSCQNNNLVTANWLR 461
           G  +   G   Q   +RG+ V + CT    T++   +AS+  V   S +   LV      
Sbjct: 789 GAKIPKLGVNAQCVSSRGDSVFLGCTNQKSTVK-KQMASSSQVQQFSIRKQRLV------ 841

Query: 462 GNSAFGQLTCSSHAHHDA 515
             S +     +SH HH A
Sbjct: 842 --STYSLPDSNSHPHHSA 857


>At3g19380.1 68416.m02458 U-box domain-containing protein contains
           similarity to immediate-early fungal elicitor protein
           CMPG1 GI:14582200 [Petroselinum crispum]; contains Pfam
           profile PF04564: U-box domain
          Length = 421

 Score = 27.5 bits (58), Expect = 6.4
 Identities = 19/87 (21%), Positives = 40/87 (45%), Gaps = 1/87 (1%)
 Frame = +1

Query: 226 AHSESTVILVSLNLYISTEEII*VQLVIQVRFVLIVGNKFLSLAQDPA-VRSVTPMLLLT 402
           AH+ + +++  L    ++ E++   L + V   +   N+F+S++ DP  V  +T +L  +
Sbjct: 148 AHNATEILIKILFSETTSSELVSESLALLVMLPITEPNQFVSISSDPGRVEFLTRLLFDS 207

Query: 403 LLSGRSAVKTIISSQPIGCVVTVLSGN 483
            +  R     +I     G     L G+
Sbjct: 208 SIETRVNAAALIEIVSTGTKSADLKGS 234


>At2g30250.1 68415.m03682 WRKY family transcription factor
          Length = 393

 Score = 27.5 bits (58), Expect = 6.4
 Identities = 15/45 (33%), Positives = 21/45 (46%)
 Frame = +3

Query: 81  KLRTDLPEPGQLAFVLNEDEFEDYLDAYLALQQSEMLANQTRNDF 215
           K +T  P P  ++   +   F DYLD+ L L  S  L + T   F
Sbjct: 45  KFKTAQPPPLPISQSSHNFTFSDYLDSPLLLSSSHSLISPTTGTF 89


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,800,577
Number of Sequences: 28952
Number of extensions: 242959
Number of successful extensions: 660
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 648
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 660
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1062855648
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -