BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fmgV12e20f
(537 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC1183.01 |sec15|SPCC1672.13|exocyst complex subunit Sec15 |Sc... 30 0.25
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 27 1.8
SPCC1840.04 |||caspase|Schizosaccharomyces pombe|chr 3|||Manual 27 2.3
SPAC2F7.10 |||palmitoyltransferase |Schizosaccharomyces pombe|ch... 26 3.1
SPAC10F6.09c |psm3|smc3|mitotic cohesin complex subunit Psm3|Sch... 26 4.1
SPAC11G7.02 |pub1||ubiquitin-protein ligase E3|Schizosaccharomyc... 25 7.2
SPAC10F6.01c ||SPAC4C5.05c|sulfite reductase beta subunit |Schiz... 25 7.2
SPBC2F12.13 |klp5|sot1|kinesin-like protein Klp5|Schizosaccharom... 25 7.2
SPCC364.06 |nap1||nucleosome assembly protein Nap1 |Schizosaccha... 25 9.5
>SPCC1183.01 |sec15|SPCC1672.13|exocyst complex subunit Sec15
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 785
Score = 29.9 bits (64), Expect = 0.25
Identities = 19/65 (29%), Positives = 33/65 (50%), Gaps = 2/65 (3%)
Frame = +1
Query: 151 SQEPSYVCL--HLILS*TMSTRAAGIFREEKTS*KFVAP*KVSTPPASAPSNWIASPTTY 324
S EP Y+CL H L S R++ R+ + +F+AP ++T + S W+ S +
Sbjct: 263 SFEPLYICLQVHSYLGLLSSFRSS-FERDRRRQQEFLAPKSLTTLDMAVVSEWLNSIAGF 321
Query: 325 SLVNF 339
+V +
Sbjct: 322 MIVEY 326
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 27.1 bits (57), Expect = 1.8
Identities = 17/58 (29%), Positives = 25/58 (43%)
Frame = -3
Query: 331 PVNKL*EMLSNSKVLKPAALTPSTEPRTFRMFSPPGRCQPPAYSLFTIGSNGDKRNWA 158
PVN L+ + +P+ P+ F+P + QPPA S S D NW+
Sbjct: 1594 PVNNSGSPLAMNAAGQPSLAVPAVPSAPSNHFNPFAKMQPPAPSPLQ-PSGHDSDNWS 1650
>SPCC1840.04 |||caspase|Schizosaccharomyces pombe|chr 3|||Manual
Length = 425
Score = 26.6 bits (56), Expect = 2.3
Identities = 12/28 (42%), Positives = 18/28 (64%)
Frame = -2
Query: 536 NRQLKFDVPDGVSVNTDSDNSTSANAHV 453
+RQ+KF D +S++ DN TSA+ V
Sbjct: 339 SRQVKFSPADVISLSGCKDNQTSADTSV 366
>SPAC2F7.10 |||palmitoyltransferase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 642
Score = 26.2 bits (55), Expect = 3.1
Identities = 12/29 (41%), Positives = 14/29 (48%)
Frame = -2
Query: 506 GVSVNTDSDNSTSANAHVGHGDSSRSVCG 420
G V NS+ N H HG R+VCG
Sbjct: 539 GTLVPPTKQNSSPKNGHGIHGSFLRTVCG 567
>SPAC10F6.09c |psm3|smc3|mitotic cohesin complex subunit
Psm3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1194
Score = 25.8 bits (54), Expect = 4.1
Identities = 22/79 (27%), Positives = 38/79 (48%), Gaps = 3/79 (3%)
Frame = +3
Query: 198 NEYAGGWHLPGGENILKVRGSVEGVNAAGFSTFE---LDSISYNLFTGQLHLSLSLNSVA 368
++YA H G + R +G AG+ + LD+I N+ T Q+ S +
Sbjct: 631 SQYARS-HQLNGITLSGDRSDKKGALTAGYRDYRNSRLDAIK-NVKTYQIKFS----DLQ 684
Query: 369 ASIDAAEGEVQIFDRKLTA 425
S++ E++ FD+K+TA
Sbjct: 685 ESLEKCRSEIESFDQKITA 703
>SPAC11G7.02 |pub1||ubiquitin-protein ligase E3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 767
Score = 25.0 bits (52), Expect = 7.2
Identities = 12/27 (44%), Positives = 14/27 (51%)
Frame = -3
Query: 283 PAALTPSTEPRTFRMFSPPGRCQPPAY 203
PAA S+EPRTF F PP +
Sbjct: 185 PAASPASSEPRTFSSFEDQYGRLPPGW 211
>SPAC10F6.01c ||SPAC4C5.05c|sulfite reductase beta subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1473
Score = 25.0 bits (52), Expect = 7.2
Identities = 12/24 (50%), Positives = 15/24 (62%)
Frame = +3
Query: 75 VAAVPYGDVDLAESQRIVDGIIEN 146
VAA PY DVD+ + + IIEN
Sbjct: 1106 VAAPPYNDVDVYTNDVALIAIIEN 1129
>SPBC2F12.13 |klp5|sot1|kinesin-like protein
Klp5|Schizosaccharomyces pombe|chr 2|||Manual
Length = 883
Score = 25.0 bits (52), Expect = 7.2
Identities = 13/40 (32%), Positives = 22/40 (55%)
Frame = +3
Query: 81 AVPYGDVDLAESQRIVDGIIENWISRAQLRLSPFDPIVNN 200
A Y DV L + ++ IIEN + AQ ++ F P++ +
Sbjct: 556 ATMYADV-LQDEVDLLKSIIENQVLDAQNKVDEFTPVLES 594
>SPCC364.06 |nap1||nucleosome assembly protein Nap1
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 393
Score = 24.6 bits (51), Expect = 9.5
Identities = 9/25 (36%), Positives = 13/25 (52%)
Frame = -2
Query: 491 TDSDNSTSANAHVGHGDSSRSVCGQ 417
+DSD S + H HG + + C Q
Sbjct: 368 SDSDEEDSDSKHTAHGQQNAAECRQ 392
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,839,397
Number of Sequences: 5004
Number of extensions: 34146
Number of successful extensions: 130
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 127
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 130
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 222442660
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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