Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fmgV12e18r
(705 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g05970.1 68416.m00681 long-chain-fatty-acid--CoA ligase / lon... 53 2e-07
At3g23790.1 68416.m02990 AMP-binding protein, putative similar t... 50 1e-06
At5g27600.1 68418.m03305 AMP-binding protein, putative similar t... 50 2e-06
At4g14070.1 68417.m02172 AMP-binding protein, putative similar t... 42 3e-04
At2g04350.2 68415.m00434 long-chain-fatty-acid--CoA ligase famil... 41 7e-04
At2g04350.1 68415.m00433 long-chain-fatty-acid--CoA ligase famil... 41 7e-04
At3g48990.1 68416.m05351 AMP-dependent synthetase and ligase fam... 40 0.002
At5g16370.1 68418.m01913 AMP-binding protein, putative similar t... 36 0.026
At5g16340.1 68418.m01910 AMP-binding protein, putative similar t... 36 0.026
At2g47240.1 68415.m05899 long-chain-fatty-acid--CoA ligase famil... 35 0.060
At1g49430.1 68414.m05541 long-chain-fatty-acid--CoA ligase / lon... 33 0.18
At3g21240.1 68416.m02684 4-coumarate--CoA ligase 2 / 4-coumaroyl... 33 0.24
At1g65890.1 68414.m07477 acyl-activating enzyme 12 (AAE12) simil... 32 0.32
At1g51680.2 68414.m05823 4-coumarate--CoA ligase 1 / 4-coumaroyl... 32 0.32
At1g51680.1 68414.m05822 4-coumarate--CoA ligase 1 / 4-coumaroyl... 32 0.32
At3g21230.1 68416.m02683 4-coumarate--CoA ligase, putative / 4-c... 32 0.43
At1g62940.1 68414.m07107 4-coumarate--CoA ligase family protein ... 31 0.56
At5g63380.1 68418.m07955 4-coumarate--CoA ligase family protein ... 31 0.74
At4g23850.1 68417.m03429 long-chain-fatty-acid--CoA ligase / lon... 31 0.74
At1g75960.1 68414.m08822 AMP-binding protein, putative similar t... 31 0.74
At1g66120.1 68414.m07504 acyl-activating enzyme 11 (AAE11) simil... 31 0.74
At5g47690.1 68418.m05887 expressed protein 31 0.98
At4g19010.1 68417.m02802 4-coumarate--CoA ligase family protein ... 31 0.98
At1g68270.1 68414.m07798 AMP-dependent synthetase and ligase fam... 31 0.98
At1g65880.1 68414.m07476 AMP-dependent synthetase and ligase fam... 31 0.98
At3g16910.1 68416.m02162 AMP-dependent synthetase and ligase fam... 30 1.3
At1g20480.1 68414.m02552 4-coumarate--CoA ligase family protein ... 30 1.3
At4g05160.1 68417.m00775 4-coumarate--CoA ligase, putative / 4-c... 29 2.3
At3g56890.1 68416.m06328 F-box family protein-related predicted ... 29 3.0
At1g65060.1 68414.m07375 4-coumarate--CoA ligase 3 / 4-coumaroyl... 29 3.0
At1g65060.2 68414.m07376 4-coumarate--CoA ligase 3 / 4-coumaroyl... 29 4.0
At5g08440.1 68418.m00994 expressed protein 28 5.2
At3g16170.1 68416.m02041 acyl-activating enzyme 13 (AAE13) simil... 28 5.2
At1g20560.1 68414.m02563 AMP-dependent synthetase and ligase fam... 28 5.2
At1g20510.2 68414.m02556 4-coumarate--CoA ligase family protein ... 28 5.2
At1g20510.1 68414.m02555 4-coumarate--CoA ligase family protein ... 28 5.2
At5g38120.1 68418.m04592 4-coumarate--CoA ligase family protein ... 28 6.9
At5g20200.1 68418.m02406 nucleoporin-related contains weak simil... 28 6.9
At2g17650.1 68415.m02042 AMP-dependent synthetase and ligase fam... 28 6.9
At4g33280.1 68417.m04735 transcriptional factor B3 family protei... 27 9.2
>At3g05970.1 68416.m00681 long-chain-fatty-acid--CoA ligase /
long-chain acyl-CoA synthetase (LACS6) strong similarity
to AMP-binding protein (MF39P) gi:1617274 from Brassica
napus, similar to putative long-chain-fatty-acid--CoA
ligase (brain isozyme) GB:P33124 [Rattus norvegicus];
contains Pfam AMP-binding enzyme domain PF00501;
identical to cDNA AtLACS6 for long-chain acyl-CoA
synthetase GI:22531705
Length = 701
Score = 52.8 bits (121), Expect = 2e-07
Identities = 54/179 (30%), Positives = 80/179 (44%), Gaps = 1/179 (0%)
Frame = -3
Query: 664 WLVLSGDVGRVDSNNLLYITGRIKELLITAGGENVAPVLIEQAIQAELLHVGYAVLIGDR 485
WL +GD+G L I R K + A GE +AP IE + A+ VG + GD
Sbjct: 528 WLH-TGDIGLWLPGGRLKIIDRKKNIFKLAQGEYIAPEKIEN-VYAKCKFVGQCFIYGD- 584
Query: 484 RKFLSVLLTLKTKVSPETGEPLDELESEARKWVASLGSSATKLSEIVNSKDPAVHKAIEA 305
F S L+ + V P D L+S W AS G L E+ N+ P V A+ +
Sbjct: 585 -SFNSSLVAV-VSVDP------DVLKS----WAASEGIKGGDLRELCNN--PRVKAAVLS 630
Query: 304 GITRANKHA-ISNAQKVQKFAILPSDFSVYTGELGPTLKIKRNVVYEKYKDIIEDFYKE 131
+ + A + + + ++ F++ G L PT KIKR E + + I + YKE
Sbjct: 631 DMDTVGREAQLRGFEFAKAVTLVLEPFTLENGLLTPTFKIKRPQAKEYFAEAITNMYKE 689
>At3g23790.1 68416.m02990 AMP-binding protein, putative similar to
AMP-binding protein GB:CAA96521 from [Brassica napus]
(Plant Mol. Biol. (1997) 33 (5), 911-922); contains Pfam
AMP-binding enzyme domain PF00501; identical to cDNA
acyl-CoA synthetase-like protein GI:20799732
Length = 722
Score = 50.4 bits (115), Expect = 1e-06
Identities = 44/178 (24%), Positives = 81/178 (45%), Gaps = 1/178 (0%)
Frame = -3
Query: 664 WLVLSGDVGRVDS-NNLLYITGRIKELLITAGGENVAPVLIEQAIQAELLHVGYAVLIGD 488
W+ GR S ++ + GR K+ ++ + GENV P+ IE+A L V+ D
Sbjct: 565 WITPQHSTGRSRSCGGVIVLEGRAKDTIVLSTGENVEPLEIEEAAMRSNLIQQIVVIGQD 624
Query: 487 RRKFLSVLLTLKTKVSPETGEPLDELESEARKWVASLGSSATKLSEIVNSKDPAVHKAIE 308
+R+ ++++ K + E A++ ++ + S +L SK+ E
Sbjct: 625 QRRLGAIVIPNK-----------EAAEGAAKQKISPVDSEVNEL-----SKETITSMVYE 668
Query: 307 AGITRANKHAISNAQKVQKFAILPSDFSVYTGELGPTLKIKRNVVYEKYKDIIEDFYK 134
K + +V I+ F++ G + PT+KI+R+ V ++YK+ IE YK
Sbjct: 669 ----ELRKWTSQCSFQVGPVLIVDEPFTIDNGLMTPTMKIRRDKVVDQYKNEIERLYK 722
>At5g27600.1 68418.m03305 AMP-binding protein, putative similar to
AMP-binding protein (MF39P) gi:1617274 from Brassica
napus, long-chain-fatty-acid--CoA ligase - Brassica
napus, EMBL:Z72152; contains Pfam AMP-binding enzyme
domain PF00501
Length = 700
Score = 49.6 bits (113), Expect = 2e-06
Identities = 50/179 (27%), Positives = 74/179 (41%), Gaps = 1/179 (0%)
Frame = -3
Query: 664 WLVLSGDVGRVDSNNLLYITGRIKELLITAGGENVAPVLIEQAIQAELLHVGYAVLIGDR 485
WL +GD+G L I R K + A GE +AP IE + + V + GD
Sbjct: 528 WLH-TGDIGLWLPGGRLKIIDRKKNIFKLAQGEYIAPEKIEN-VYTKCRFVSQCFIHGD- 584
Query: 484 RKFLSVLLTLKTKVSPETGEPLDELESEARKWVASLGSSATKLSEIVNSKDPAVHKAIEA 305
F S L+ + V PE + W AS G L ++ N DP V K + A
Sbjct: 585 -SFNSSLVAI-VSVDPEV----------MKDWAASEGIKYEHLGQLCN--DPRVRKTVLA 630
Query: 304 GITRANKHA-ISNAQKVQKFAILPSDFSVYTGELGPTLKIKRNVVYEKYKDIIEDFYKE 131
+ + A + + + ++P F++ G L PT KIKR + + I Y E
Sbjct: 631 EMDDLGREAQLRGFEFAKAVTLVPEPFTLENGLLTPTFKIKRPQAKAYFAEAISKMYAE 689
>At4g14070.1 68417.m02172 AMP-binding protein, putative similar to
AMP-binding protein [gi:1617272] from Brassica napus;
contains Pfam AMP-binding enzyme domain PF00501;
identical to cDNA acyl-CoA synthetase-like protein
GI:20799730
Length = 727
Score = 42.3 bits (95), Expect = 3e-04
Identities = 30/100 (30%), Positives = 49/100 (49%), Gaps = 6/100 (6%)
Frame = -3
Query: 664 WLVLSGDVGRV-DSNNLLYITGRIKELLITAGGENVAPVLIEQAIQAELLHVGYAVLIGD 488
W+ GR ++ + GR K+ ++ + GENV P+ IE+A + V+ D
Sbjct: 581 WIAPHHSKGRSRHCGGVIVLEGRAKDTIVLSTGENVEPLEIEEAAMRSRVIEQIVVIGQD 640
Query: 487 RRKFLSVLLTLK---TKVSPETG-EPLDEL-ESEARKWVA 383
RR+ ++++ K +V PET E L L E RKW +
Sbjct: 641 RRRLGAIIIPNKEEAQRVDPETSKETLKSLVYQELRKWTS 680
Score = 34.7 bits (76), Expect = 0.060
Identities = 14/36 (38%), Positives = 24/36 (66%)
Frame = -3
Query: 244 ILPSDFSVYTGELGPTLKIKRNVVYEKYKDIIEDFY 137
I+ F++ G + PT+KI+R++V KYK+ I+ Y
Sbjct: 691 IVDDPFTIDNGLMTPTMKIRRDMVVAKYKEEIDQLY 726
>At2g04350.2 68415.m00434 long-chain-fatty-acid--CoA ligase family
protein / long-chain acyl-CoA synthetase family protein
(LACS8) similar to LACS 4 [SP|O35547] from Rattus
norvegicus, LACS 4 [SP|O60488] from Homo sapiens;
contains Pfam HMM hit: AMP-binding enzymes PF00501
Length = 720
Score = 41.1 bits (92), Expect = 7e-04
Identities = 45/173 (26%), Positives = 77/173 (44%), Gaps = 1/173 (0%)
Frame = -3
Query: 652 SGDVGRVDSNNLLYITGRIKELLITAGGENVAPVLIEQAIQAELLHVGYAVLIGDRRKFL 473
+GD+GR + L + R K+++ GE V+ +E A+ + +V ++ D
Sbjct: 563 TGDIGRFHPDGCLEVIDRKKDIVKLQHGEYVSLGKVEAALGSS-NYVDNIMVHADPINSY 621
Query: 472 SVLLTLKTKVSPETGEPLDELESEARKWVASLGSSATKLSEIVNSKDPAVHKAIEAGITR 293
V L V P G LE KW G ++ +E+ K AV K ++ +T+
Sbjct: 622 CVAL-----VVPSRG----ALE----KWAEEAGVKHSEFAELC-EKGEAV-KEVQQSLTK 666
Query: 292 ANKHA-ISNAQKVQKFAILPSDFSVYTGELGPTLKIKRNVVYEKYKDIIEDFY 137
A K A + + K +L ++ +G + LKIKR + K+KD + Y
Sbjct: 667 AGKAAKLEKFELPAKIKLLSEPWTPESGLVTAALKIKREQIKSKFKDELSKLY 719
>At2g04350.1 68415.m00433 long-chain-fatty-acid--CoA ligase family
protein / long-chain acyl-CoA synthetase family protein
(LACS8) similar to LACS 4 [SP|O35547] from Rattus
norvegicus, LACS 4 [SP|O60488] from Homo sapiens;
contains Pfam HMM hit: AMP-binding enzymes PF00501
Length = 720
Score = 41.1 bits (92), Expect = 7e-04
Identities = 45/173 (26%), Positives = 77/173 (44%), Gaps = 1/173 (0%)
Frame = -3
Query: 652 SGDVGRVDSNNLLYITGRIKELLITAGGENVAPVLIEQAIQAELLHVGYAVLIGDRRKFL 473
+GD+GR + L + R K+++ GE V+ +E A+ + +V ++ D
Sbjct: 563 TGDIGRFHPDGCLEVIDRKKDIVKLQHGEYVSLGKVEAALGSS-NYVDNIMVHADPINSY 621
Query: 472 SVLLTLKTKVSPETGEPLDELESEARKWVASLGSSATKLSEIVNSKDPAVHKAIEAGITR 293
V L V P G LE KW G ++ +E+ K AV K ++ +T+
Sbjct: 622 CVAL-----VVPSRG----ALE----KWAEEAGVKHSEFAELC-EKGEAV-KEVQQSLTK 666
Query: 292 ANKHA-ISNAQKVQKFAILPSDFSVYTGELGPTLKIKRNVVYEKYKDIIEDFY 137
A K A + + K +L ++ +G + LKIKR + K+KD + Y
Sbjct: 667 AGKAAKLEKFELPAKIKLLSEPWTPESGLVTAALKIKREQIKSKFKDELSKLY 719
>At3g48990.1 68416.m05351 AMP-dependent synthetase and ligase family
protein similar to peroxisomal-coenzyme A synthetase
(FAT2) [gi:586339] from Saccharomyces cerevisiae;
contains Pfam AMP-binding enzyme domain PF00501;
identical to cDNA; identical to cDNA adenosine
monophosphate binding protein 3 AMPBP3
(AMPBP3)GI:20799714
Length = 514
Score = 39.9 bits (89), Expect = 0.002
Identities = 16/40 (40%), Positives = 28/40 (70%)
Frame = -3
Query: 652 SGDVGRVDSNNLLYITGRIKELLITAGGENVAPVLIEQAI 533
+GD+G D++ L++ GRIKE LI GGE ++P+ ++ +
Sbjct: 392 TGDIGYFDTDGYLHLVGRIKE-LINRGGEKISPIEVDAVL 430
>At5g16370.1 68418.m01913 AMP-binding protein, putative similar to
AMP-binding protein GI:1903034 from [Brassica napus];
contains Pfam AMP-binding domain PF00501; identical to
cDNA adenosine monophosphate binding protein 5 AMPBP5
(AMPBP5) GI:20799718
Length = 552
Score = 35.9 bits (79), Expect = 0.026
Identities = 18/40 (45%), Positives = 27/40 (67%)
Frame = -3
Query: 652 SGDVGRVDSNNLLYITGRIKELLITAGGENVAPVLIEQAI 533
+GDVG + S+ L I R K+++IT GGENV+ V +E +
Sbjct: 421 TGDVGVIHSDGYLEIKDRSKDIIIT-GGENVSSVEVETVL 459
>At5g16340.1 68418.m01910 AMP-binding protein, putative similar to
AMP-binding protein GI:1903034 from [Brassica napus];
contains Pfam AMP-binding domain PF00501; identical to
adenosine monophosphate binding protein 6 AMPBP6
(AMPBP6) GI:20799720
Length = 550
Score = 35.9 bits (79), Expect = 0.026
Identities = 18/40 (45%), Positives = 27/40 (67%)
Frame = -3
Query: 652 SGDVGRVDSNNLLYITGRIKELLITAGGENVAPVLIEQAI 533
+GDVG + S+ L I R K+++IT GGENV+ V +E +
Sbjct: 421 TGDVGVIHSDGYLEIKDRSKDIIIT-GGENVSSVEVETVL 459
>At2g47240.1 68415.m05899 long-chain-fatty-acid--CoA ligase family
protein / long-chain acyl-CoA synthetase family protein
similar to GI:1617270 (MF7P) and gi:1617628 (MF45P) from
[Brassica napus] ; contains Pfam AMP-binding enzyme
domain PF00501
Length = 660
Score = 34.7 bits (76), Expect = 0.060
Identities = 41/174 (23%), Positives = 74/174 (42%)
Frame = -3
Query: 652 SGDVGRVDSNNLLYITGRIKELLITAGGENVAPVLIEQAIQAELLHVGYAVLIGDRRKFL 473
+GD+G + N +L I R K L+ + GE VA +E + V + GD K +
Sbjct: 497 TGDIGEILPNGVLKIIDRKKNLIKLSQGEYVALEHLENIFGQNSV-VQDIWVYGDSFKSM 555
Query: 472 SVLLTLKTKVSPETGEPLDELESEARKWVASLGSSATKLSEIVNSKDPAVHKAIEAGITR 293
V + + +PET +W LG + E+ + + H E T
Sbjct: 556 LVAVVVP---NPET----------VNRWAKDLGFTKP-FEELCSFPELKEHIISELKST- 600
Query: 292 ANKHAISNAQKVQKFAILPSDFSVYTGELGPTLKIKRNVVYEKYKDIIEDFYKE 131
A K+ + + ++ + F V + TLK +RN + + Y+ I++ Y++
Sbjct: 601 AEKNKLRKFEYIKAVTVETKPFDVERDLVTATLKNRRNNLLKYYQVQIDEMYRK 654
>At1g49430.1 68414.m05541 long-chain-fatty-acid--CoA ligase /
long-chain acyl-CoA synthetase nearly identical to acyl
CoA synthetase (MF45P) GI:1617268 from [Brassica napus]
Length = 665
Score = 33.1 bits (72), Expect = 0.18
Identities = 13/54 (24%), Positives = 28/54 (51%)
Frame = -3
Query: 292 ANKHAISNAQKVQKFAILPSDFSVYTGELGPTLKIKRNVVYEKYKDIIEDFYKE 131
A ++ + + ++ + P+ F + + PT K+KR + + YK I++ Y E
Sbjct: 606 AKQYQLKGFEMLKAIHLEPNPFDIERDLITPTFKLKRPQLLQHYKGIVDQLYSE 659
>At3g21240.1 68416.m02684 4-coumarate--CoA ligase 2 /
4-coumaroyl-CoA synthase 2 (4CL2) identical to SP|Q9S725
4-coumarate--CoA ligase 2 (EC 6.2.1.12) (4CL 2)
(4-coumaroyl-CoA synthase 2) {Arabidopsis thaliana}
Length = 556
Score = 32.7 bits (71), Expect = 0.24
Identities = 19/44 (43%), Positives = 27/44 (61%)
Frame = -3
Query: 664 WLVLSGDVGRVDSNNLLYITGRIKELLITAGGENVAPVLIEQAI 533
WL +GDVG +D ++ L+I R+KE LI G VAP +E +
Sbjct: 429 WLH-TGDVGFIDDDDELFIVDRLKE-LIKYKGFQVAPAELESLL 470
>At1g65890.1 68414.m07477 acyl-activating enzyme 12 (AAE12) similar
to AMP-binding protein GI:1903034 from [Brassica napus];
contains Pfam AMP-binding domain PF00501; identical to
cDNA acyl-activating enzyme 12 (At1g65890) mRNA
GI:29893228, acyl-activating enzyme 12 [Arabidopsis
thaliana] GI:29893229
Length = 578
Score = 32.3 bits (70), Expect = 0.32
Identities = 18/44 (40%), Positives = 28/44 (63%)
Frame = -3
Query: 664 WLVLSGDVGRVDSNNLLYITGRIKELLITAGGENVAPVLIEQAI 533
WL SGDVG + + + I R K+++I+ GGEN++ V +E I
Sbjct: 421 WLN-SGDVGVIHPDGHVEIKDRSKDIIIS-GGENISSVEVENII 462
>At1g51680.2 68414.m05823 4-coumarate--CoA ligase 1 /
4-coumaroyl-CoA synthase 1 (4CL1) identical to SP|Q42524
4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4CL 1)
(4-coumaroyl-CoA synthase 1) {Arabidopsis thaliana}
Length = 490
Score = 32.3 bits (70), Expect = 0.32
Identities = 18/41 (43%), Positives = 26/41 (63%)
Frame = -3
Query: 664 WLVLSGDVGRVDSNNLLYITGRIKELLITAGGENVAPVLIE 542
WL +GD+G +D ++ L+I R+KE LI G VAP +E
Sbjct: 436 WLH-TGDIGLIDDDDELFIVDRLKE-LIKYKGFQVAPAELE 474
>At1g51680.1 68414.m05822 4-coumarate--CoA ligase 1 /
4-coumaroyl-CoA synthase 1 (4CL1) identical to SP|Q42524
4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4CL 1)
(4-coumaroyl-CoA synthase 1) {Arabidopsis thaliana}
Length = 561
Score = 32.3 bits (70), Expect = 0.32
Identities = 18/41 (43%), Positives = 26/41 (63%)
Frame = -3
Query: 664 WLVLSGDVGRVDSNNLLYITGRIKELLITAGGENVAPVLIE 542
WL +GD+G +D ++ L+I R+KE LI G VAP +E
Sbjct: 436 WLH-TGDIGLIDDDDELFIVDRLKE-LIKYKGFQVAPAELE 474
>At3g21230.1 68416.m02683 4-coumarate--CoA ligase, putative /
4-coumaroyl-CoA synthase, putative (4CL) similar to 4CL2
[gi:12229665] and 4CL1 [gi:12229649] from [Arabidopsis
thaliana], 4CL1 [gi:12229631] from Nicotiana tabacum
Length = 570
Score = 31.9 bits (69), Expect = 0.43
Identities = 18/41 (43%), Positives = 26/41 (63%)
Frame = -3
Query: 664 WLVLSGDVGRVDSNNLLYITGRIKELLITAGGENVAPVLIE 542
WL +GD+G VD ++ ++I R+KE LI G VAP +E
Sbjct: 443 WLH-TGDIGFVDDDDEIFIVDRLKE-LIKFKGYQVAPAELE 481
>At1g62940.1 68414.m07107 4-coumarate--CoA ligase family protein /
4-coumaroyl-CoA synthase family protein similar to
gi:112801 from Petroselinum crispum, GB:AAD40664 from
[Solanum tuberosum] (J. Biol. Chem. 266 (13), 8551-8559
(1991)); contains Pfam AMP-binding enzyme domain PF00501
Length = 542
Score = 31.5 bits (68), Expect = 0.56
Identities = 18/41 (43%), Positives = 25/41 (60%)
Frame = -3
Query: 664 WLVLSGDVGRVDSNNLLYITGRIKELLITAGGENVAPVLIE 542
WL +GD+G +D + ++I RIKE LI G VAP +E
Sbjct: 415 WLH-TGDIGYIDDDGDIFIVDRIKE-LIKYKGFQVAPAELE 453
>At5g63380.1 68418.m07955 4-coumarate--CoA ligase family protein /
4-coumaroyl-CoA synthase family protein similar to 4CL2
[gi:12229665] from Arabidopsis thaliana, 4CL1
[gi:12229631] from Nicotiana tabacum; contains Pfam
AMP-binding enzyme domain PF00501
Length = 562
Score = 31.1 bits (67), Expect = 0.74
Identities = 19/46 (41%), Positives = 26/46 (56%)
Frame = -3
Query: 664 WLVLSGDVGRVDSNNLLYITGRIKELLITAGGENVAPVLIEQAIQA 527
WL +GD+ DS + LYI R+KE LI V PV +EQ + +
Sbjct: 433 WLK-TGDLCYFDSEDFLYIVDRLKE-LIKYKAYQVPPVELEQILHS 476
>At4g23850.1 68417.m03429 long-chain-fatty-acid--CoA ligase /
long-chain acyl-CoA synthetase nearly identical to
acyl-CoA synthetase (MF7P) from Brassica napus
[gi:1617270]
Length = 666
Score = 31.1 bits (67), Expect = 0.74
Identities = 43/177 (24%), Positives = 71/177 (40%)
Frame = -3
Query: 664 WLVLSGDVGRVDSNNLLYITGRIKELLITAGGENVAPVLIEQAIQAELLHVGYAVLIGDR 485
WL +GDVG + + I R K + + GE VA IE I E+ V + G+
Sbjct: 497 WLH-TGDVGEWQPDGSMKIIDRKKNIFKLSQGEYVAVENIEN-IYGEVQAVDSVWVYGN- 553
Query: 484 RKFLSVLLTLKTKVSPETGEPLDELESEARKWVASLGSSATKLSEIVNSKDPAVHKAIEA 305
F S L+ + P + +W A G S + N K A +
Sbjct: 554 -SFESFLIAI--------ANPNQHI---LERWAAENGVSGDYDALCQNEK--AKEFILGE 599
Query: 304 GITRANKHAISNAQKVQKFAILPSDFSVYTGELGPTLKIKRNVVYEKYKDIIEDFYK 134
+ A + + + ++ + P F + L PT K KR + + Y+ +I++ YK
Sbjct: 600 LVKMAKEKKMKGFEIIKAIHLDPVPFDMERDLLTPTFKKKRPQLLKYYQSVIDEMYK 656
>At1g75960.1 68414.m08822 AMP-binding protein, putative similar to
AMP-binding protein GI:1903034 from [Brassica napus];
contains Pfam profile: PF00501 AMP-binding enzyme;
identical to cDNA adenosine monophosphate binding
protein 8 AMPBP8 (AMPBP8) GI:20799724
Length = 544
Score = 31.1 bits (67), Expect = 0.74
Identities = 15/40 (37%), Positives = 26/40 (65%)
Frame = -3
Query: 652 SGDVGRVDSNNLLYITGRIKELLITAGGENVAPVLIEQAI 533
+GD+G + + L I R K+++I+ GGENV+ V +E +
Sbjct: 421 TGDLGVIHGDGYLEIKDRSKDVIIS-GGENVSSVEVEAVL 459
>At1g66120.1 68414.m07504 acyl-activating enzyme 11 (AAE11) similar
to AMP-binding protein GI:1903034 from [Brassica napus];
contains Pfam AMP-binding domain PF00501; identical to
cDNA acyl-activating enzyme 11 (At1g66120) GI:29893230,
acyl-activating enzyme 11 [Arabidopsis thaliana]
GI:29893231
Length = 572
Score = 31.1 bits (67), Expect = 0.74
Identities = 14/44 (31%), Positives = 29/44 (65%)
Frame = -3
Query: 664 WLVLSGDVGRVDSNNLLYITGRIKELLITAGGENVAPVLIEQAI 533
WL +GD+G + + + I R K+++I+ GGEN++ + +E+ +
Sbjct: 421 WLN-TGDIGVIHPDGYVEIKDRSKDIIIS-GGENISSIEVEKVL 462
>At5g47690.1 68418.m05887 expressed protein
Length = 1638
Score = 30.7 bits (66), Expect = 0.98
Identities = 26/99 (26%), Positives = 40/99 (40%)
Frame = -3
Query: 436 ETGEPLDELESEARKWVASLGSSATKLSEIVNSKDPAVHKAIEAGITRANKHAISNAQKV 257
E G LD + L +A LSE+ S PAV K+I+ + K I N Q
Sbjct: 11 ELGSKLDLAPVSKDSLLKLLKEAAVCLSELEQSPPPAVLKSIQPFLDAVIKPEILNHQDK 70
Query: 256 QKFAILPSDFSVYTGELGPTLKIKRNVVYEKYKDIIEDF 140
++ S S T P N++ + ++ I+ F
Sbjct: 71 DVKLLVASCVSEITRITAPEAPYSDNIMKDIFQLIVSAF 109
>At4g19010.1 68417.m02802 4-coumarate--CoA ligase family protein /
4-coumaroyl-CoA synthase family protein similar to 4CL
from Pinus taeda, gi:515503, gi:1143308; contains Pfam
AMP-binding enzyme domain PF00501
Length = 566
Score = 30.7 bits (66), Expect = 0.98
Identities = 19/55 (34%), Positives = 29/55 (52%)
Frame = -3
Query: 664 WLVLSGDVGRVDSNNLLYITGRIKELLITAGGENVAPVLIEQAIQAELLHVGYAV 500
WL +GD+ D + L+I RIKE +I G +AP +E + + L + AV
Sbjct: 437 WL-RTGDIAYFDEDGYLFIVDRIKE-IIKYKGFQIAPADLEAVLVSHPLIIDAAV 489
>At1g68270.1 68414.m07798 AMP-dependent synthetase and ligase family
protein similar to AMP-binding protein GI:1903034 from
[Brassica napus]; contains Pfam AMP-binding domain
PF00501
Length = 535
Score = 30.7 bits (66), Expect = 0.98
Identities = 16/44 (36%), Positives = 28/44 (63%)
Frame = -3
Query: 664 WLVLSGDVGRVDSNNLLYITGRIKELLITAGGENVAPVLIEQAI 533
WL +GDVG + + + I R K+++I+ GGEN++ V +E +
Sbjct: 391 WLN-TGDVGVIHPDGHIEIKDRSKDIIIS-GGENISSVEVENIL 432
>At1g65880.1 68414.m07476 AMP-dependent synthetase and ligase family
protein similar to AMP-binding protein GI:1903034 from
[Brassica napus]; contains Pfam AMP-binding domain
PF00501
Length = 580
Score = 30.7 bits (66), Expect = 0.98
Identities = 16/44 (36%), Positives = 28/44 (63%)
Frame = -3
Query: 664 WLVLSGDVGRVDSNNLLYITGRIKELLITAGGENVAPVLIEQAI 533
WL +GDVG + + + I R K+++I+ GGEN++ V +E +
Sbjct: 421 WLN-TGDVGVIHPDGHVEIKDRSKDIIIS-GGENISSVEVENVL 462
>At3g16910.1 68416.m02162 AMP-dependent synthetase and ligase family
protein similar to AMP-binding protein GI:1903034 from
[Brassica napus]; contains Pfam AMP-binding domain
PF00501; identical to cDNA adenosine monophosphate
binding protein 7 AMPBP7 (AMPBP7) GI:20799722
Length = 569
Score = 30.3 bits (65), Expect = 1.3
Identities = 14/40 (35%), Positives = 25/40 (62%)
Frame = -3
Query: 652 SGDVGRVDSNNLLYITGRIKELLITAGGENVAPVLIEQAI 533
SGD+ +N + I R K+++I+ GGEN++ V +E +
Sbjct: 436 SGDIAVKHPDNYIEIKDRSKDVIIS-GGENISSVEVENVV 474
>At1g20480.1 68414.m02552 4-coumarate--CoA ligase family protein /
4-coumaroyl-CoA synthase family protein similar to
SP|Q9S725 from Arabidopsis thaliana and SP|P17814 from
Oryza sativa; contains Pfam AMP-binding enzyme domain
PF00501
Length = 565
Score = 30.3 bits (65), Expect = 1.3
Identities = 16/46 (34%), Positives = 26/46 (56%)
Frame = -3
Query: 664 WLVLSGDVGRVDSNNLLYITGRIKELLITAGGENVAPVLIEQAIQA 527
WL +GD+ +D + +++ R+KE LI G VAP +E + A
Sbjct: 440 WLK-TGDLCYIDGDGFVFVVDRLKE-LIKCNGYQVAPAELEALLLA 483
>At4g05160.1 68417.m00775 4-coumarate--CoA ligase, putative /
4-coumaroyl-CoA synthase, putative similar to 4CL2
[gi:12229665] from Arabidopsis thaliana, 4CL1
[gi:12229631] from Nicotiana tabacum; contains Pfam
AMP-binding enzyme domain PF00501; acyl-activating
enzyme superfamily; identical to cDNA 4-coumarate-CoA
ligase-like protein (At4g05160) GI:29893226
Length = 544
Score = 29.5 bits (63), Expect = 2.3
Identities = 17/39 (43%), Positives = 23/39 (58%)
Frame = -3
Query: 658 VLSGDVGRVDSNNLLYITGRIKELLITAGGENVAPVLIE 542
V +GD+G + + LY+ RIKE LI G VAP +E
Sbjct: 420 VHTGDLGYFNEDGNLYVVDRIKE-LIKYKGFQVAPAELE 457
>At3g56890.1 68416.m06328 F-box family protein-related predicted
proteins - Arabidopsis thaliana; contains TIGRFAM
TIGR01640: F-box protein interaction domain
Length = 219
Score = 29.1 bits (62), Expect = 3.0
Identities = 15/44 (34%), Positives = 26/44 (59%)
Frame = -3
Query: 604 GRIKELLITAGGENVAPVLIEQAIQAELLHVGYAVLIGDRRKFL 473
G+IK+L+ ++GGE + +L+ I A GYA + + KF+
Sbjct: 4 GKIKDLVNSSGGERIETILMVLCIIAVKGLKGYASMGSEEFKFI 47
>At1g65060.1 68414.m07375 4-coumarate--CoA ligase 3 /
4-coumaroyl-CoA synthase 3 (4CL3) identical to SP|Q9S777
4-coumarate--CoA ligase 3 (EC 6.2.1.12) (4CL 3)
(4-coumaroyl-CoA synthase 3) {Arabidopsis thaliana}
Length = 561
Score = 29.1 bits (62), Expect = 3.0
Identities = 16/44 (36%), Positives = 26/44 (59%)
Frame = -3
Query: 664 WLVLSGDVGRVDSNNLLYITGRIKELLITAGGENVAPVLIEQAI 533
WL +GD+G VD ++ ++I R+KE +I G V P +E +
Sbjct: 439 WLH-TGDIGYVDEDDEIFIVDRLKE-VIKFKGFQVPPAELESLL 480
Score = 27.5 bits (58), Expect = 9.2
Identities = 10/20 (50%), Positives = 12/20 (60%)
Frame = -1
Query: 705 LNDAEKTKGAIDDDGWYSPG 646
LND E T ID++GW G
Sbjct: 424 LNDPEATSATIDEEGWLHTG 443
>At1g65060.2 68414.m07376 4-coumarate--CoA ligase 3 /
4-coumaroyl-CoA synthase 3 (4CL3) identical to SP|Q9S777
4-coumarate--CoA ligase 3 (EC 6.2.1.12) (4CL 3)
(4-coumaroyl-CoA synthase 3) {Arabidopsis thaliana}
Length = 495
Score = 28.7 bits (61), Expect = 4.0
Identities = 16/51 (31%), Positives = 29/51 (56%)
Frame = -3
Query: 664 WLVLSGDVGRVDSNNLLYITGRIKELLITAGGENVAPVLIEQAIQAELLHV 512
WL +GD+G VD ++ ++I R+KE++ G + + I I + LH+
Sbjct: 439 WLH-TGDIGYVDEDDEIFIVDRLKEVIKFKGFQASPFIFIVLFIFNKCLHL 488
Score = 27.5 bits (58), Expect = 9.2
Identities = 10/20 (50%), Positives = 12/20 (60%)
Frame = -1
Query: 705 LNDAEKTKGAIDDDGWYSPG 646
LND E T ID++GW G
Sbjct: 424 LNDPEATSATIDEEGWLHTG 443
>At5g08440.1 68418.m00994 expressed protein
Length = 726
Score = 28.3 bits (60), Expect = 5.2
Identities = 13/23 (56%), Positives = 15/23 (65%)
Frame = -1
Query: 294 AQISMPYLTLKKYKSLRSFPQTS 226
A++ YL L KYKS S PQTS
Sbjct: 60 AELQRRYLELAKYKSGESLPQTS 82
>At3g16170.1 68416.m02041 acyl-activating enzyme 13 (AAE13) similar
to malonyl CoA synthetase GB:AAF28840 from
[Bradyrhizobium japonicum]; contains Pfam AMP-binding
enzyme domain PF00501; identical to cDNA acyl-activating
enzyme 13 (At3g16170) GI:29893232, acyl-activating
enzyme 13 [Arabidopsis thaliana] GI:29893233
Length = 544
Score = 28.3 bits (60), Expect = 5.2
Identities = 13/40 (32%), Positives = 21/40 (52%)
Frame = -3
Query: 652 SGDVGRVDSNNLLYITGRIKELLITAGGENVAPVLIEQAI 533
+GD GRVD + I GR ++ GG ++ + IE +
Sbjct: 411 TGDAGRVDEDGYYVILGRNSADIMKVGGYKLSALEIESTL 450
>At1g20560.1 68414.m02563 AMP-dependent synthetase and ligase family
protein similar to AMP-binding protein GI:1903034 from
[Brassica napus]; contains Pfam AMP-binding domain
PF00501; identical to adenosine monophosphate binding
protein 1 AMPBP1 (AMPBP1) GI:20799710
Length = 556
Score = 28.3 bits (60), Expect = 5.2
Identities = 12/40 (30%), Positives = 25/40 (62%)
Frame = -3
Query: 652 SGDVGRVDSNNLLYITGRIKELLITAGGENVAPVLIEQAI 533
SGD+G + + + R K+++I+ GGEN++ + +E +
Sbjct: 425 SGDLGVKHPDGYIELKDRSKDIIIS-GGENISSIEVESTL 463
>At1g20510.2 68414.m02556 4-coumarate--CoA ligase family protein /
4-coumaroyl-CoA synthase family protein similar to
SP|P14912 and SP|P14913 from Petroselinum crispum;
contains Pfam AMP-binding enzyme domain PF00501
Length = 473
Score = 28.3 bits (60), Expect = 5.2
Identities = 15/41 (36%), Positives = 24/41 (58%)
Frame = -3
Query: 664 WLVLSGDVGRVDSNNLLYITGRIKELLITAGGENVAPVLIE 542
WL +GD+ +D + +++ R+KE LI G VAP +E
Sbjct: 419 WL-RTGDLCYIDEDGFIFVVDRLKE-LIKYKGYQVAPAELE 457
>At1g20510.1 68414.m02555 4-coumarate--CoA ligase family protein /
4-coumaroyl-CoA synthase family protein similar to
SP|P14912 and SP|P14913 from Petroselinum crispum;
contains Pfam AMP-binding enzyme domain PF00501
Length = 546
Score = 28.3 bits (60), Expect = 5.2
Identities = 15/41 (36%), Positives = 24/41 (58%)
Frame = -3
Query: 664 WLVLSGDVGRVDSNNLLYITGRIKELLITAGGENVAPVLIE 542
WL +GD+ +D + +++ R+KE LI G VAP +E
Sbjct: 419 WL-RTGDLCYIDEDGFIFVVDRLKE-LIKYKGYQVAPAELE 457
>At5g38120.1 68418.m04592 4-coumarate--CoA ligase family protein /
4-coumaroyl-CoA synthase family protein similar to 4CL2,
Arabidopsis thaliana [gi:12229665], 4CL1, Nicotiana
tabacum [gi:12229631]; contains Pfam AMP-binding enzyme
domain PF00501
Length = 550
Score = 27.9 bits (59), Expect = 6.9
Identities = 16/41 (39%), Positives = 24/41 (58%)
Frame = -3
Query: 664 WLVLSGDVGRVDSNNLLYITGRIKELLITAGGENVAPVLIE 542
WL +GD+ +D++ L+I R+KE LI G V P +E
Sbjct: 425 WLK-TGDLCYIDNDGFLFIVDRLKE-LIKYKGYQVPPAELE 463
>At5g20200.1 68418.m02406 nucleoporin-related contains weak
similarity to Nucleoporin NUP1 (Nuclear pore protein
NUP1) (Swiss-Prot:P20676) [Saccharomyces cerevisiae]
Length = 762
Score = 27.9 bits (59), Expect = 6.9
Identities = 16/43 (37%), Positives = 21/43 (48%)
Frame = -3
Query: 334 DPAVHKAIEAGITRANKHAISNAQKVQKFAILPSDFSVYTGEL 206
DPA ++ I G TR + SNA A P D + + GEL
Sbjct: 60 DPA-YRIISGGATRILPYFFSNAASAPALAAPPEDQNQHQGEL 101
>At2g17650.1 68415.m02042 AMP-dependent synthetase and ligase family
protein similar to AMP-binding protein GI:1903034 from
[Brassica napus]; contains Pfam AMP-binding domain
PF00501; identical to cDNA adenosine monophosphate
binding protein 2 AMPBP2 (AMPBP2) GI:20799712
Length = 603
Score = 27.9 bits (59), Expect = 6.9
Identities = 13/40 (32%), Positives = 26/40 (65%)
Frame = -3
Query: 652 SGDVGRVDSNNLLYITGRIKELLITAGGENVAPVLIEQAI 533
SGD+ + + I R+K+++I+ GGEN++ V +E+ +
Sbjct: 479 SGDLAVKYPDGYIEIKDRLKDVIIS-GGENISSVEVERVL 517
>At4g33280.1 68417.m04735 transcriptional factor B3 family protein
contains Pfam profile PF02362: B3 DNA binding domain
Length = 461
Score = 27.5 bits (58), Expect = 9.2
Identities = 21/74 (28%), Positives = 36/74 (48%), Gaps = 6/74 (8%)
Frame = +3
Query: 186 LIFNVGPNSPVYTEKSEGRIANFCTF*ALDMACLF--ARVIPASIALWTAGSLLLTI--- 350
L F N +YT+ + ++NFC F + + C F ++ +SI + T +LL I
Sbjct: 52 LCFPPAFNLEIYTQMYQRGLSNFCFFLSKSVLCGFKSPKIANSSIKITTFCNLLQVIPRK 111
Query: 351 -SLSLVAELPKLAT 389
S +LP++ T
Sbjct: 112 FSTHCKRKLPQIVT 125
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,127,504
Number of Sequences: 28952
Number of extensions: 246897
Number of successful extensions: 810
Number of sequences better than 10.0: 40
Number of HSP's better than 10.0 without gapping: 780
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 808
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1516419560
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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