BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fmgV12d14r (686 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC4B3.11c |||conserved eukaryotic protein|Schizosaccharomyces ... 64 1e-11 SPAC8C9.11 |||BolA domain protein|Schizosaccharomyces pombe|chr ... 48 1e-06 SPAC1952.01 ||SPAC1B3.19|Pig-U|Schizosaccharomyces pombe|chr 1||... 29 0.83 SPAC4G8.12c |||alpha-1,2-mannosyltransferase |Schizosaccharomyce... 27 1.9 SPBC16D10.08c |||heat shock protein Hsp104 |Schizosaccharomyces ... 27 2.5 SPAC1002.14 |itt1||ubiquitin-protein ligase E3 |Schizosaccharomy... 27 2.5 >SPCC4B3.11c |||conserved eukaryotic protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 116 Score = 64.5 bits (150), Expect = 1e-11 Identities = 28/70 (40%), Positives = 47/70 (67%) Frame = -3 Query: 660 EQQLTDALKKAMPGITYISVQDISGGCGAMFEISVEAKEFVGLNRVKQHRLVTDSLKNEI 481 E+++ D L + + + + V D+SGGCG+M+++++++K F G N + QHRLV LK EI Sbjct: 33 ERRIKDILTEKLSPSS-LRVIDVSGGCGSMYQVAIKSKAFQGKNTLAQHRLVNSILKEEI 91 Query: 480 AEMHGIRIHT 451 MHG+ + T Sbjct: 92 RNMHGLNLST 101 >SPAC8C9.11 |||BolA domain protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 80 Score = 48.4 bits (110), Expect = 1e-06 Identities = 20/49 (40%), Positives = 32/49 (65%) Frame = -3 Query: 615 TYISVQDISGGCGAMFEISVEAKEFVGLNRVKQHRLVTDSLKNEIAEMH 469 T+I +QD+SGGCG FE+ + + F G + + +HRLV L+ I ++H Sbjct: 18 THIEIQDMSGGCGQNFEVIIVSPLFEGKSTLARHRLVNHKLQEVIKDIH 66 >SPAC1952.01 ||SPAC1B3.19|Pig-U|Schizosaccharomyces pombe|chr 1|||Manual Length = 408 Score = 28.7 bits (61), Expect = 0.83 Identities = 12/59 (20%), Positives = 28/59 (47%) Frame = -2 Query: 223 ETNKSMCTCRDITNLMVLIIHMLQVSWNCITQANSLSSFVFLLTFYVHNC*HHKLLICC 47 + N+ + TC + ++ +++ L A+ + +F L+T Y +C + + CC Sbjct: 115 DPNQVLSTCSPLWISVIYLLNPLTFLPGIACSADMILNFTTLMTIYFASCGSYAIYACC 173 >SPAC4G8.12c |||alpha-1,2-mannosyltransferase |Schizosaccharomyces pombe|chr 1|||Manual Length = 533 Score = 27.5 bits (58), Expect = 1.9 Identities = 21/80 (26%), Positives = 38/80 (47%), Gaps = 3/80 (3%) Frame = +1 Query: 319 LQYKYTCLMAITVFLCIFSRNSSFQKI*MLFPSSAIFSLRGGRRRVNTDA---MHFCYFI 489 + Y YT L+AI L F+R + + FS+R ++ VN +H C F+ Sbjct: 168 ISYLYTFLLAIVSVLGFFTRITFLA---FVIAPYIYFSVRCFKKNVNNPKDIFLHLCIFV 224 Query: 490 LQRICY*SVLFHSVQPYEFF 549 + + +VL ++ Y+F+ Sbjct: 225 --SVSFATVLACILEDYKFY 242 >SPBC16D10.08c |||heat shock protein Hsp104 |Schizosaccharomyces pombe|chr 2|||Manual Length = 905 Score = 27.1 bits (57), Expect = 2.5 Identities = 12/32 (37%), Positives = 19/32 (59%) Frame = +1 Query: 19 LNKISYCYHYNILTIYDVNNYVHKRSIKIQKK 114 LN+IS +N L D+ N V R +++QK+ Sbjct: 773 LNRISSIVIFNRLRRVDIRNIVENRILEVQKR 804 >SPAC1002.14 |itt1||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 435 Score = 27.1 bits (57), Expect = 2.5 Identities = 11/34 (32%), Positives = 17/34 (50%) Frame = +2 Query: 293 WLTFTSKRIFSISILV*WQ*LYFYVFFPEIRHFR 394 WL KR+ + S++ W ++ V F I H R Sbjct: 96 WLPLKQKRVLTSSLIKLWNEIHDCVLFDAIEHVR 129 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,998,657 Number of Sequences: 5004 Number of extensions: 64832 Number of successful extensions: 143 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 137 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 143 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 317927284 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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