Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fmgV12d07r
(725 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC24C6.06 |gpa1||G-protein alpha subunit |Schizosaccharomyces ... 137 1e-33
SPAC23H3.13c |gpa2|git8|heterotrimeric G protein alpha-2 subunit... 119 4e-28
SPBC31F10.06c |sar1||ADP-ribosylation factor Sar1|Schizosaccharo... 38 0.002
SPAC22F3.05c |alp41||ADP-ribosylation factor Alp41|Schizosacchar... 37 0.003
SPBC4F6.18c |arf1||ADP-ribosylation factor Arf1|Schizosaccharomy... 36 0.004
SPBC1539.08 |||ADP-ribosylation factor, Arf family|Schizosacchar... 33 0.031
SPBC530.04 |mod5||Tea1 anchoring protein Mod5|Schizosaccharomyce... 32 0.073
SPCC1235.15 |dga1|SPCC548.01|diacylglycerol O-acyltransferase |S... 31 0.17
SPCC1682.15 |mug122||PX/PXA domain protein|Schizosaccharomyces p... 29 0.51
SPBC4.07c |rpt2|mts2|19S proteasome regulatory subunit Rpt2|Schi... 28 1.2
SPCC777.03c |||nifs homolog|Schizosaccharomyces pombe|chr 3|||Ma... 28 1.2
SPBC1D7.05 |byr2|ste8, SPBC2F12.01|MAP kinase kinase kinase Byr2... 27 2.1
SPBC16H5.13 |||WD repeat protein |Schizosaccharomyces pombe|chr ... 27 2.1
SPAPB1E7.07 |glt1||glutamate synthase Glt1 |Schizosaccharomyces ... 27 2.7
SPBC651.10 |nse5||Smc5-6 complex non-SMC subunit Nse5|Schizosacc... 27 2.7
SPAPB1A10.09 |ase1||microtubule-associated protein Ase1 |Schizos... 27 3.6
SPCC830.07c |psi1|psi|DNAJ domain protein Psi1|Schizosaccharomyc... 26 4.8
SPCC364.07 ||SPCC4G3.01|D-3 phosphoglycerate dehydrogenase |Schi... 26 6.3
SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 26 6.3
SPBC3E7.10 |fma1||methionine aminopeptidase Fma1 |Schizosaccharo... 26 6.3
SPBC1773.10c |||asparagine-tRNA ligase Ded81 |Schizosaccharomyce... 26 6.3
SPBC31F10.09c |nut2|med10|mediator complex subunit Med10|Schizos... 25 8.3
>SPBC24C6.06 |gpa1||G-protein alpha subunit |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 407
Score = 137 bits (332), Expect = 1e-33
Identities = 87/232 (37%), Positives = 124/232 (53%), Gaps = 1/232 (0%)
Frame = -1
Query: 698 IDSAEYFLDRIDLIEKEDYIPSDSDILRCRQKTTGIQKIEFKVKIPKSMQGGVQDFWMFD 519
+D+ Y+ D ID I YIPSD DIL CR KTTGI + F + + FD
Sbjct: 189 LDNFYYYQDHIDRIFDPQYIPSDQDILHCRIKTTGISEETFLLNR--------HHYRFFD 240
Query: 518 VGGQRGERKKWIQVFEGINAIWFLVACSDFDQTLREDNTTNRLQESLNLFEDVWQSRFLL 339
VGGQR ER+KWI FE + A+ FLV+ + +DQ L EDN+ N++QE+L L++ + S +
Sbjct: 241 VGGQRSERRKWIHCFENVTALLFLVSLAGYDQCLVEDNSGNQMQEALLLWDSICNSSWFS 300
Query: 338 EAGLIVFLNKQDVLEAKVSRGRSP-ATHFPEYSRYEAPGDEYTRTKLFIRSLFVDLTKRQ 162
E+ +I+FLNK D+ + KV SP HFP+Y E T F+++ L
Sbjct: 301 ESAMILFLNKLDLFKRKVH--ISPIQKHFPDY-------QEVGSTPTFVQTQ-CPLADNA 350
Query: 161 RQRAVAASAERFVVGEAHRPRECYFHFTTATDTDNVRTVFRDVHQIILTHVL 6
+ + +F R CY HFTTATDT ++ V V I+++ L
Sbjct: 351 VRSGMYYFYLKFESLNRIASRSCYCHFTTATDTSLLQRVMVSVQDTIMSNNL 402
>SPAC23H3.13c |gpa2|git8|heterotrimeric G protein alpha-2 subunit
Gpa2 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 354
Score = 119 bits (287), Expect = 4e-28
Identities = 85/239 (35%), Positives = 123/239 (51%), Gaps = 3/239 (1%)
Frame = -1
Query: 713 NEYQXIDSAEYFLDRIDLIEKEDYIPSDSDILRCRQKTTGIQKIEFKVKIPKSMQGGVQD 534
N+ IDSA YF R D I Y+P+ DILR R T GI +I F + +Q
Sbjct: 142 NDSVLIDSAPYFFSRADEICSRHYVPTIDDILRSRNSTLGISEISFTLD---HLQ----- 193
Query: 533 FWMFDVGGQRGERKKWIQVFEGINAIWFLVACSDFDQTL--REDNTTNRLQESLNLFEDV 360
MFDVGGQR ER+KWI FE +N+I F V+ +D+D+ L R NRL ES++LF+ +
Sbjct: 194 IRMFDVGGQRTERRKWIYCFENVNSIIFCVSLNDYDKKLYERAAPERNRLVESISLFDSI 253
Query: 359 WQSRFLLEAGLIVFLNKQDVLEAKVSRGRSPATHFPEY-SRYEAPGDEYTRTKLFIRSLF 183
S++ + + +I+FLNK D+ K+ F +Y +YE + T+ +I LF
Sbjct: 254 INSQWFMHSSIILFLNKFDLFRKKLEH-----VPFQDYFPQYEGKNSVKSITR-YILWLF 307
Query: 182 VDLTKRQRQRAVAASAERFVVGEAHRPRECYFHFTTATDTDNVRTVFRDVHQIILTHVL 6
V+ + + + + Y H TTA DT N++ VF V + IL H L
Sbjct: 308 VNPSINRAKHNI------------------YPHITTAVDTSNIKVVFSAVKETILQHSL 348
>SPBC31F10.06c |sar1||ADP-ribosylation factor
Sar1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 190
Score = 37.5 bits (83), Expect = 0.002
Identities = 15/37 (40%), Positives = 22/37 (59%)
Frame = -1
Query: 533 FWMFDVGGQRGERKKWIQVFEGINAIWFLVACSDFDQ 423
F FD+GG + R+ W F +N I +LV C DF++
Sbjct: 66 FTTFDLGGHQQARRLWRDYFPEVNGIVYLVDCCDFER 102
>SPAC22F3.05c |alp41||ADP-ribosylation factor
Alp41|Schizosaccharomyces pombe|chr 1|||Manual
Length = 186
Score = 36.7 bits (81), Expect = 0.003
Identities = 28/97 (28%), Positives = 42/97 (43%), Gaps = 1/97 (1%)
Frame = -1
Query: 533 FWMFDVGGQRGERKKWIQVFEGINA-IWFLVACSDFDQTLREDNTTNRLQESLNLFEDVW 357
F ++D+GGQ+ R W FE A IW + + D RL+E N +++
Sbjct: 62 FTIWDIGGQKTLRNFWKNYFESTEAIIWVVDSLDDL-----------RLEECRNTLQELL 110
Query: 356 QSRFLLEAGLIVFLNKQDVLEAKVSRGRSPATHFPEY 246
LL ++V NK DV A S S + +Y
Sbjct: 111 VEEKLLFTSILVLANKSDVSGALSSEEISKILNISKY 147
>SPBC4F6.18c |arf1||ADP-ribosylation factor Arf1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 180
Score = 36.3 bits (80), Expect = 0.004
Identities = 30/81 (37%), Positives = 39/81 (48%)
Frame = -1
Query: 533 FWMFDVGGQRGERKKWIQVFEGINAIWFLVACSDFDQTLREDNTTNRLQESLNLFEDVWQ 354
F ++DVGGQ R W F+ I F+V +D + R LQ LN ED
Sbjct: 63 FTVWDVGGQDKIRPLWRHYFQNTQGIIFVVDSNDRE---RISEAHEELQRMLN--EDE-- 115
Query: 353 SRFLLEAGLIVFLNKQDVLEA 291
L +A L+VF NKQD+ A
Sbjct: 116 ---LRDALLLVFANKQDLPNA 133
>SPBC1539.08 |||ADP-ribosylation factor, Arf
family|Schizosaccharomyces pombe|chr 2|||Manual
Length = 184
Score = 33.5 bits (73), Expect = 0.031
Identities = 23/78 (29%), Positives = 36/78 (46%)
Frame = -1
Query: 533 FWMFDVGGQRGERKKWIQVFEGINAIWFLVACSDFDQTLREDNTTNRLQESLNLFEDVWQ 354
F ++DVGGQ R W F G + F+V +D +NR+ E+ +
Sbjct: 67 FNVWDVGGQDKIRPLWRHYFTGTKGLIFVVDSAD----------SNRISEARQELHRIIS 116
Query: 353 SRFLLEAGLIVFLNKQDV 300
R + + L+V NKQD+
Sbjct: 117 DREMRDCLLLVLANKQDL 134
>SPBC530.04 |mod5||Tea1 anchoring protein Mod5|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 522
Score = 32.3 bits (70), Expect = 0.073
Identities = 23/79 (29%), Positives = 30/79 (37%)
Frame = +2
Query: 221 RPRALRTGSTPGSGWRASGPGTPSPPARPAC*GTRSAQPPEETCSATRLRRGSRTPAVC* 400
RP R+ P + A G G+P PPA P+ + S E T R PA
Sbjct: 435 RPTYSRSSPLPSASVPALGDGSPDPPAAPSIQNSLSVHESEMPPHVT---RDYTQPAASA 491
Query: 401 WCCPPSKFGRSRYKPPRTR 457
P K KPP+ +
Sbjct: 492 TPVPKEKPSEKSEKPPKKK 510
>SPCC1235.15 |dga1|SPCC548.01|diacylglycerol O-acyltransferase
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 345
Score = 31.1 bits (67), Expect = 0.17
Identities = 20/45 (44%), Positives = 27/45 (60%)
Frame = +1
Query: 547 PCIDFGILTLNSIFWIPVVF*RHLSISLSDGM*SSFSIKSILSRK 681
P I+ +LTLNS F++PV R ++L+ S S SILSRK
Sbjct: 144 PGINVSVLTLNSNFYVPVY--RDYLMALNINSVSKKSCVSILSRK 186
>SPCC1682.15 |mug122||PX/PXA domain protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 749
Score = 29.5 bits (63), Expect = 0.51
Identities = 13/39 (33%), Positives = 19/39 (48%)
Frame = +1
Query: 274 RPRDTFASSTSCLLRNTISPASRRNLLCHTSSKRFKDSC 390
RP D+ CLLR ++ + + H SS RF + C
Sbjct: 214 RPEDSACLPLHCLLREVLAMQVFKRITTHCSSPRFVNRC 252
>SPBC4.07c |rpt2|mts2|19S proteasome regulatory subunit
Rpt2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 448
Score = 28.3 bits (60), Expect = 1.2
Identities = 15/42 (35%), Positives = 21/42 (50%)
Frame = -3
Query: 321 VPQQAGRAGGEGVPGPLARHPLPGVLPVRSARGRVHEDQALH 196
VP + GR + GP A LP V+P R R+ + Q +H
Sbjct: 35 VPTRTGRRKKKAQSGPDASAKLPTVIPTTRCRLRLLKMQRIH 76
>SPCC777.03c |||nifs homolog|Schizosaccharomyces pombe|chr
3|||Manual
Length = 396
Score = 28.3 bits (60), Expect = 1.2
Identities = 14/47 (29%), Positives = 23/47 (48%)
Frame = +1
Query: 439 QATKNQIALIPSNTCIHFFLSPLWPPTSNIQKSCTPPCIDFGILTLN 579
QA + Q+ L+ N I FF PP ++I + +D+G+ N
Sbjct: 11 QAFRKQVPLVEKNENITFFNLSFQPPMNSIVANTINEYVDWGLYNPN 57
>SPBC1D7.05 |byr2|ste8, SPBC2F12.01|MAP kinase kinase kinase
Byr2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 659
Score = 27.5 bits (58), Expect = 2.1
Identities = 24/85 (28%), Positives = 36/85 (42%)
Frame = +1
Query: 112 ASPTTKRSAEAATARCRCLLVKSTNRLRMKSLVLVYSSPGASYREYSGKWVAGERPRDTF 291
A P S + + + +L ST + ++S + P SY+ + + R D F
Sbjct: 167 AQPAALSSQSSLSPKLSSVLPTSTQKRSVRSN---NAKPFESYQRPPSELI-NSRISDFF 222
Query: 292 ASSTSCLLRNTISPASRRNLLCHTS 366
LL TIS + RRNL TS
Sbjct: 223 PDHQPKLLEKTISNSLRRNLSIRTS 247
>SPBC16H5.13 |||WD repeat protein |Schizosaccharomyces pombe|chr
2|||Manual
Length = 1026
Score = 27.5 bits (58), Expect = 2.1
Identities = 11/24 (45%), Positives = 16/24 (66%)
Frame = -1
Query: 89 FHFTTATDTDNVRTVFRDVHQIIL 18
+H + A TDN+ TVF D H ++L
Sbjct: 530 YHGSCALLTDNLVTVFLDAHDLVL 553
>SPAPB1E7.07 |glt1||glutamate synthase Glt1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2111
Score = 27.1 bits (57), Expect = 2.7
Identities = 12/27 (44%), Positives = 14/27 (51%)
Frame = +3
Query: 69 RGGREVEVALPGPVGLTNDEALGRGGH 149
R GR V + GP GL + L R GH
Sbjct: 1753 RTGRRVAIIGSGPAGLAAADQLNRAGH 1779
>SPBC651.10 |nse5||Smc5-6 complex non-SMC subunit
Nse5|Schizosaccharomyces pombe|chr 2|||Manual
Length = 388
Score = 27.1 bits (57), Expect = 2.7
Identities = 10/20 (50%), Positives = 14/20 (70%)
Frame = +1
Query: 481 CIHFFLSPLWPPTSNIQKSC 540
CI + P +P +SNIQ+SC
Sbjct: 44 CITLLVHPEYPTSSNIQESC 63
>SPAPB1A10.09 |ase1||microtubule-associated protein Ase1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 731
Score = 26.6 bits (56), Expect = 3.6
Identities = 21/62 (33%), Positives = 27/62 (43%), Gaps = 2/62 (3%)
Frame = +2
Query: 185 TDSV*RAWSSCTRPRALRTGSTPGSGWRASGPGTPSPPARPAC*GTR--SAQPPEETCSA 358
TDSV R S R T S + + P TP P + +R SA+PP T +A
Sbjct: 488 TDSVNRTPSQRGRVAVPSTPSVRSASRAMTSPRTPLPRVKNTQNPSRSISAEPPSATSTA 547
Query: 359 TR 364
R
Sbjct: 548 NR 549
>SPCC830.07c |psi1|psi|DNAJ domain protein Psi1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 379
Score = 26.2 bits (55), Expect = 4.8
Identities = 17/46 (36%), Positives = 21/46 (45%), Gaps = 3/46 (6%)
Frame = +2
Query: 236 RTGSTPGSGWRASGPGTPSPPAR---PAC*GTRSAQPPEETCSATR 364
R G PG G G P AR P+ G+R +QPP + TR
Sbjct: 164 RGGGMPGGFANMFGGGGAGPHARRSHPSFGGSRPSQPPAQNEVITR 209
>SPCC364.07 ||SPCC4G3.01|D-3 phosphoglycerate dehydrogenase
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 466
Score = 25.8 bits (54), Expect = 6.3
Identities = 20/56 (35%), Positives = 26/56 (46%), Gaps = 5/56 (8%)
Frame = +3
Query: 108 VGLTNDEALGR-----GGHGALPLPLGQVDEQTPYEELGPRVLVPGRFVPGVLREV 260
+G+ EAL R GA+ P + T + RVL R VPGVLR+V
Sbjct: 356 IGIEVSEALTRYINEGNSIGAVNFPEVSLRSLTEADRNAARVLFVHRNVPGVLRQV 411
>SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 800
Score = 25.8 bits (54), Expect = 6.3
Identities = 21/67 (31%), Positives = 26/67 (38%), Gaps = 4/67 (5%)
Frame = +2
Query: 272 SGPGTPSPPARPAC*GTRSAQPPEETCSATRLR----RGSRTPAVC*WCCPPSKFGRSRY 439
S TP PPA + GT S+ P + S T G+ T V P S S
Sbjct: 311 SSTSTPPPPASTSSTGTSSSPLPSTSTSCTTSTSIPPTGNSTTPVTPTVPPTSTSSTSTP 370
Query: 440 KPPRTRS 460
PP + S
Sbjct: 371 PPPASTS 377
>SPBC3E7.10 |fma1||methionine aminopeptidase Fma1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 379
Score = 25.8 bits (54), Expect = 6.3
Identities = 15/63 (23%), Positives = 28/63 (44%), Gaps = 3/63 (4%)
Frame = +3
Query: 468 TFKHLYPLFPFTPLAAHVEHPEVLYAALHRLRYLD---FELNFLDPRRFLTTSKYIAVRR 638
+ K +YPL P + H++ P+ + R ++ F+L L P+ K + R
Sbjct: 77 SLKPVYPLSPIRKVPPHIKRPDYAKTGVSRSEQIEGRSFKLKRLTPKEQEGMRKVCRLGR 136
Query: 639 NVV 647
V+
Sbjct: 137 EVL 139
>SPBC1773.10c |||asparagine-tRNA ligase Ded81 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 568
Score = 25.8 bits (54), Expect = 6.3
Identities = 20/56 (35%), Positives = 31/56 (55%), Gaps = 3/56 (5%)
Frame = +3
Query: 138 RGGHGALPLPL-GQVDEQTPYE--ELGPRVLVPGRFVPGVLREVGGGRAAPGHLRL 296
R G G L L G+V ++ Y+ LGP V + GV++E+ G++APG+ L
Sbjct: 165 RDGTGFLQCVLSGKVYDRASYDFINLGPESTV---CLYGVIKELPEGKSAPGNHEL 217
>SPBC31F10.09c |nut2|med10|mediator complex subunit
Med10|Schizosaccharomyces pombe|chr 2|||Manual
Length = 138
Score = 25.4 bits (53), Expect = 8.3
Identities = 12/37 (32%), Positives = 21/37 (56%)
Frame = -1
Query: 308 QDVLEAKVSRGRSPATHFPEYSRYEAPGDEYTRTKLF 198
QDVLE + +GR+P + ++S ++Y KL+
Sbjct: 65 QDVLEY-IEQGRNPDVYARQFSELVQKDNQYVNGKLY 100
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,930,191
Number of Sequences: 5004
Number of extensions: 62300
Number of successful extensions: 247
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 227
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 244
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 341222980
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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