Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fmgV12b14f
(625 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g14180.1 68418.m01658 lipase family protein similar to SP|Q64... 133 1e-31
At2g15230.1 68415.m01737 lipase family protein similar to SP|P07... 113 9e-26
At1g73920.2 68414.m08561 lipase family protein similar to lipase... 91 7e-19
At1g73920.1 68414.m08560 lipase family protein similar to lipase... 91 7e-19
At1g18460.1 68414.m02303 lipase family protein similar to triacy... 86 2e-17
At1g49580.1 68414.m05559 calcium-dependent protein kinase, putat... 36 0.022
At1g77670.1 68414.m09043 aminotransferase class I and II family ... 33 0.15
At2g26720.1 68415.m03205 plastocyanin-like domain-containing pro... 31 0.82
At1g65380.1 68414.m07417 receptor-like protein CLAVATA2 (CLV2) i... 31 0.82
At5g40210.1 68418.m04879 nodulin MtN21 family protein similar to... 30 1.4
At1g32240.1 68414.m03966 myb family transcription factor (KAN2) ... 29 1.9
At4g34570.1 68417.m04912 bifunctional dihydrofolate reductase-th... 29 3.3
At3g19100.1 68416.m02427 calcium-dependent protein kinase, putat... 29 3.3
At2g16370.1 68415.m01873 bifunctional dihydrofolate reductase-th... 29 3.3
At1g65630.1 68414.m07444 DegP protease, putative contains simila... 27 7.7
>At5g14180.1 68418.m01658 lipase family protein similar to SP|Q64194
Lysosomal acid lipase/cholesteryl ester hydrolase
precursor (EC 3.1.1.13) {Rattus norvegicus}; contains
Pfam profile PF04083: ab-hydrolase associated lipase
region
Length = 418
Score = 133 bits (321), Expect = 1e-31
Identities = 63/142 (44%), Positives = 89/142 (62%), Gaps = 1/142 (0%)
Frame = +2
Query: 188 YGYPVEVHKVVTEDGYILEMQRIPHGRDQNNRPDP-RKPAVLVMHGLFASAADWVLMGPG 364
+GY E H VVT+DGYIL MQRIP GR D ++ VL+ HG+ W+L
Sbjct: 59 FGYKCEEHDVVTQDGYILNMQRIPEGRAGAVAGDGGKRQPVLIQHGILVDGMSWLLNPAD 118
Query: 365 LGLAYILAEAGYDVWLGNARGTYYSRAHTKLDPDNDSEFWKFSWEEIGTRDLPAMIDYTL 544
L ILA+ G+DVW+GN RGT +SR H L+P + FW ++W+E+ + DLPAM D+
Sbjct: 119 QNLPLILADQGFDVWMGNTRGTRFSRRHKYLNP-SQRAFWNWTWDELVSYDLPAMFDHIH 177
Query: 545 KVAGKRRLHYIGHSQGTTVFWA 610
+ G +++HY+GHS GT + +A
Sbjct: 178 GLTG-QKIHYLGHSLGTLIGFA 198
>At2g15230.1 68415.m01737 lipase family protein similar to SP|P07098
Triacylglycerol lipase, gastric precursor (EC 3.1.1.3)
{Homo sapiens}; contains Pfam profile PF04083:
ab-hydrolase associated lipase region
Length = 393
Score = 113 bits (272), Expect = 9e-26
Identities = 58/145 (40%), Positives = 80/145 (55%)
Frame = +2
Query: 176 LITKYGYPVEVHKVVTEDGYILEMQRIPHGRDQNNRPDPRKPAVLVMHGLFASAADWVLM 355
LI Y H + T+DGYIL +QR+ + P VL+ HGLF + W L
Sbjct: 36 LIHPANYSCTEHSIQTKDGYILALQRVASLGPRLQSGPP----VLLQHGLFMAGDVWFLN 91
Query: 356 GPGLGLAYILAEAGYDVWLGNARGTYYSRAHTKLDPDNDSEFWKFSWEEIGTRDLPAMID 535
P L +ILA+ G+DVW+GN RGT YS H L D D EFW +SW+++ DL MI
Sbjct: 92 SPKESLGFILADHGFDVWVGNVRGTRYSYGHVTLS-DTDKEFWDWSWQDLAMYDLAEMIQ 150
Query: 536 YTLKVAGKRRLHYIGHSQGTTVFWA 610
Y ++ ++ +GHSQGT + +A
Sbjct: 151 YLYSISNS-KIFLVGHSQGTIMSFA 174
>At1g73920.2 68414.m08561 lipase family protein similar to lipase
GB:CAA74737 [SP|O46108] from [Drosophila melanogaster]
Length = 635
Score = 90.6 bits (215), Expect = 7e-19
Identities = 52/120 (43%), Positives = 71/120 (59%)
Frame = +2
Query: 176 LITKYGYPVEVHKVVTEDGYILEMQRIPHGRDQNNRPDPRKPAVLVMHGLFASAADWVLM 355
+IT+ GYP E +V+T DGY+L ++RIP R D RK AV + HG+ S+ WV
Sbjct: 230 VITELGYPYEAIRVITSDGYVLVLERIP-------RRDARK-AVFLQHGVLDSSMGWVSN 281
Query: 356 GPGLGLAYILAEAGYDVWLGNARGTYYSRAHTKLDPDNDSEFWKFSWEEIGTRDLPAMID 535
G A+ + GYDV+LGN RG SR H + + EFW++S E GT D+PAMI+
Sbjct: 282 GVVGSPAFAAYDQGYDVFLGNFRG-LVSRDHVNKN-ISSKEFWRYSINEHGTEDIPAMIE 339
>At1g73920.1 68414.m08560 lipase family protein similar to lipase
GB:CAA74737 [SP|O46108] from [Drosophila melanogaster]
Length = 704
Score = 90.6 bits (215), Expect = 7e-19
Identities = 52/120 (43%), Positives = 71/120 (59%)
Frame = +2
Query: 176 LITKYGYPVEVHKVVTEDGYILEMQRIPHGRDQNNRPDPRKPAVLVMHGLFASAADWVLM 355
+IT+ GYP E +V+T DGY+L ++RIP R D RK AV + HG+ S+ WV
Sbjct: 299 VITELGYPYEAIRVITSDGYVLVLERIP-------RRDARK-AVFLQHGVLDSSMGWVSN 350
Query: 356 GPGLGLAYILAEAGYDVWLGNARGTYYSRAHTKLDPDNDSEFWKFSWEEIGTRDLPAMID 535
G A+ + GYDV+LGN RG SR H + + EFW++S E GT D+PAMI+
Sbjct: 351 GVVGSPAFAAYDQGYDVFLGNFRG-LVSRDHVNKN-ISSKEFWRYSINEHGTEDIPAMIE 408
>At1g18460.1 68414.m02303 lipase family protein similar to
triacylglycerol lipase, gastric precursor (EC 3.1.1.3)
{Canis familiaris} [SP|P80035]
Length = 701
Score = 85.8 bits (203), Expect = 2e-17
Identities = 52/120 (43%), Positives = 70/120 (58%)
Frame = +2
Query: 176 LITKYGYPVEVHKVVTEDGYILEMQRIPHGRDQNNRPDPRKPAVLVMHGLFASAADWVLM 355
+IT+ GYP E +VVT DGY L ++RIP R D RK AV + HG+ S+ WV
Sbjct: 297 VITELGYPYEAIRVVTSDGYGLLLERIP-------RRDARK-AVYLQHGVMDSSMGWVSN 348
Query: 356 GPGLGLAYILAEAGYDVWLGNARGTYYSRAHTKLDPDNDSEFWKFSWEEIGTRDLPAMID 535
G A+ + GYDV+LGN RG SR H K + + +FW++S E T D+PAMI+
Sbjct: 349 GVVGSPAFAAYDQGYDVFLGNFRG-LVSRDHVKKN-ISSKDFWRYSINEHATEDIPAMIE 406
>At1g49580.1 68414.m05559 calcium-dependent protein kinase, putative
/ CDPK, putative similar to calcium/calmodulin-dependent
protein kinase CaMK3 [Nicotiana tabacum]
gi|16904226|gb|AAL30820; contains protein kinase domain,
Pfam:PF00069; contains serine/threonine protein kinase
domain, INTERPRO:IPR002290
Length = 606
Score = 35.9 bits (79), Expect = 0.022
Identities = 36/148 (24%), Positives = 62/148 (41%), Gaps = 16/148 (10%)
Frame = +2
Query: 95 EELYRLDAQGSRYSTNVTEDALLDIVGLITKYGYPVEVHKVVTEDGYIL-------EMQR 253
E L R+ A+G +YS N + ++ I+ ++ + VH+ + + ++ +++
Sbjct: 238 ELLDRILARGGKYSENDAKPVIIQILNVVAFCHFQGVVHRDLKPENFLYTSKEENSQLKA 297
Query: 254 IPHGRDQNNRPDPRKPAVL---------VMHGLFASAADWVLMGPGLGLAYILAEAGYDV 406
I G RPD R ++ V+H + + AD +G +AYIL
Sbjct: 298 IDFGLSDFVRPDERLNDIVGSAYYVAPEVLHRSYTTEADVWSIGV---IAYILLCGSRPF 354
Query: 407 WLGNARGTYYSRAHTKLDPDNDSEFWKF 490
W G + RA K DP D W F
Sbjct: 355 WARTESGIF--RAVLKADPSFDEPPWPF 380
>At1g77670.1 68414.m09043 aminotransferase class I and II family
protein similar to kynurenine aminotransferase
/glutamine transaminase K GI:1030066 [Rattus norvegicus]
Length = 440
Score = 33.1 bits (72), Expect = 0.15
Identities = 20/65 (30%), Positives = 32/65 (49%), Gaps = 1/65 (1%)
Frame = +2
Query: 383 LAEAGYDVWLGNARGTYYSRA-HTKLDPDNDSEFWKFSWEEIGTRDLPAMIDYTLKVAGK 559
L E G+ V+ + GTY+ A HT +ND F ++ EE+G +P + Y GK
Sbjct: 354 LKEVGFTVFPSS--GTYFVVADHTPFGMENDVAFCEYLIEEVGVVAIPTSVFYLNPEEGK 411
Query: 560 RRLHY 574
+ +
Sbjct: 412 NLVRF 416
>At2g26720.1 68415.m03205 plastocyanin-like domain-containing
protein / mavicyanin, putative similar to mavicyanin
SP:P80728 from [Cucurbita pepo]
Length = 206
Score = 30.7 bits (66), Expect = 0.82
Identities = 13/26 (50%), Positives = 18/26 (69%)
Frame = -2
Query: 396 PASAKMYAKPRPGPIRTQSAAEAKSP 319
PAS A P PGP+R+QS++ + SP
Sbjct: 127 PASLGHVAVPVPGPVRSQSSSSSPSP 152
>At1g65380.1 68414.m07417 receptor-like protein CLAVATA2 (CLV2)
identical to receptor-like protein CLAVATA2 [Arabidopsis
thaliana] gi|6049566|gb|AAF02654contains leucine
rich-repeat domains Pfam:PF00560, INTERPRO:IPR001611;
Length = 720
Score = 30.7 bits (66), Expect = 0.82
Identities = 22/73 (30%), Positives = 41/73 (56%), Gaps = 5/73 (6%)
Frame = +3
Query: 36 FSVSVLPRRLFADPLMRITSRNYTGWTLRVPVTLPM*LKMLFWILLDLSRNM---DIQLK 206
F+ + PR +F++ L+ + ++ G++ R+P + + L +LLDLS N DI L+
Sbjct: 276 FNYEISPRLMFSEKLVML-DLSHNGFSGRLPSRISETTEKLGLVLLDLSHNSFSGDIPLR 334
Query: 207 FT--RSLLKMAIS 239
T +SL + +S
Sbjct: 335 ITELKSLQALRLS 347
>At5g40210.1 68418.m04879 nodulin MtN21 family protein similar to
MtN21 GI:2598575 (root nodule development) from
[Medicago truncatula]
Length = 339
Score = 29.9 bits (64), Expect = 1.4
Identities = 18/47 (38%), Positives = 25/47 (53%), Gaps = 2/47 (4%)
Frame = +2
Query: 305 VLVMHG--LFASAADWVLMGPGLGLAYILAEAGYDVWLGNARGTYYS 439
V + HG L +S +DW++ G L L YIL Y V + + G Y S
Sbjct: 153 VTLYHGPMLMSSHSDWIIGGGLLALQYILVSVSYLV-MAHTMGRYPS 198
>At1g32240.1 68414.m03966 myb family transcription factor (KAN2)
contains Pfam profile: PF00249 myb-like DNA-binding
domain; identical to cDNA GARP-like putative
transcription factor KANADI2 (KAN2) GI:15723594
Length = 388
Score = 29.5 bits (63), Expect = 1.9
Identities = 21/57 (36%), Positives = 29/57 (50%), Gaps = 1/57 (1%)
Frame = -3
Query: 494 N*ISKTLSHCPGLI*CELWSNMYHVRFQATHHNQLQPKCMLSQDLDPSE-PSQQQKQ 327
N SKT++H L + +H + Q HH QL P C S L + P+QQQ+Q
Sbjct: 59 NSTSKTINH--PFQDLSLSNISHHQQQQQHHHPQLLPNCNSSNILTSFQFPTQQQQQ 113
>At4g34570.1 68417.m04912 bifunctional dihydrofolate
reductase-thymidylate synthase 2 / DHFR-TS (THY-2)
identical to SP|Q05763
Length = 565
Score = 28.7 bits (61), Expect = 3.3
Identities = 16/57 (28%), Positives = 23/57 (40%), Gaps = 1/57 (1%)
Frame = +2
Query: 374 AYILAEAGYDVWLGNARGTYYSR-AHTKLDPDNDSEFWKFSWEEIGTRDLPAMIDYT 541
A +L E G +W GNA Y T+ + + + F W G + DYT
Sbjct: 350 AKVLQEKGIRIWDGNASRAYLDGIGLTEREEGDLGPVYGFQWRHFGAKYTDMHADYT 406
>At3g19100.1 68416.m02427 calcium-dependent protein kinase, putative
/ CDPK, putative similar to calcium/calmodulin-dependent
protein kinase CaMK3 [Nicotiana tabacum]
gi|16904226|gb|AAL30820; contains protein kinase domain,
Pfam:PF00069; contains serine/threonine protein kinase
domain, INTERPRO:IPR002290
Length = 599
Score = 28.7 bits (61), Expect = 3.3
Identities = 35/146 (23%), Positives = 59/146 (40%), Gaps = 16/146 (10%)
Frame = +2
Query: 95 EELYRLDAQGSRYSTNVTEDALLDIVGLITKYGYPVEVHKVVTEDGYILE-------MQR 253
E L R+ A+G +YS + + L+ I+ ++ VH+ + + ++ ++
Sbjct: 232 ELLDRILARGGKYSEDDAKAVLIQILNVVAFCHLQGVVHRDLKPENFLYTSKEENSMLKV 291
Query: 254 IPHGRDQNNRPDPRKPAVL---------VMHGLFASAADWVLMGPGLGLAYILAEAGYDV 406
I G RPD R ++ V+H + + AD +G +AYIL
Sbjct: 292 IDFGLSDFVRPDERLNDIVGSAYYVAPEVLHRSYTTEADVWSIGV---IAYILLCGSRPF 348
Query: 407 WLGNARGTYYSRAHTKLDPDNDSEFW 484
W G + RA K DP D W
Sbjct: 349 WARTESGIF--RAVLKADPSFDEPPW 372
>At2g16370.1 68415.m01873 bifunctional dihydrofolate
reductase-thymidylate synthase 1 / DHFR-TS (THY-1)
identical to GP:289193:L08593 [SP|Q05762]
Length = 519
Score = 28.7 bits (61), Expect = 3.3
Identities = 16/57 (28%), Positives = 23/57 (40%), Gaps = 1/57 (1%)
Frame = +2
Query: 374 AYILAEAGYDVWLGNARGTYYSR-AHTKLDPDNDSEFWKFSWEEIGTRDLPAMIDYT 541
A +L E G +W GNA Y T+ + + + F W G + DYT
Sbjct: 304 AKVLQEKGIHIWDGNASREYLDGIGLTEREEGDLGPVYGFQWRHFGAKYTDMHADYT 360
>At1g65630.1 68414.m07444 DegP protease, putative contains
similarity to DegP2 protease GI:13172275 from
[Arabidopsis thaliana]
Length = 559
Score = 27.5 bits (58), Expect = 7.7
Identities = 13/34 (38%), Positives = 19/34 (55%)
Frame = +2
Query: 176 LITKYGYPVEVHKVVTEDGYILEMQRIPHGRDQN 277
LI K +VHKV+ +D IL + +P G D +
Sbjct: 325 LINKINPLSDVHKVLKKDDIILAIDGVPIGNDSS 358
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,751,440
Number of Sequences: 28952
Number of extensions: 325866
Number of successful extensions: 843
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 814
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 831
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1265787216
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -