BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fmgV12b01f
(546 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g35070.1 68417.m04978 expressed protein 29 2.0
At5g18180.1 68418.m02134 Gar1 RNA-binding region family protein ... 28 3.5
At5g41520.1 68418.m05044 40S ribosomal protein S10 (RPS10B) cont... 27 8.2
At4g14280.1 68417.m02201 hypothetical protein 27 8.2
At2g36910.1 68415.m04527 multidrug resistance P-glycoprotein (PG... 27 8.2
>At4g35070.1 68417.m04978 expressed protein
Length = 265
Score = 29.1 bits (62), Expect = 2.0
Identities = 13/46 (28%), Positives = 21/46 (45%)
Frame = +3
Query: 318 CRGRGRFNFRHSPVQKLGTKRISLQCTNLGTLRLICPPVKHMCICL 455
C G G N P +K+ C + G R++ P +H+C C+
Sbjct: 197 CGGEGDGN--SLPAKKMKMSSCCCNCGSNGVTRVLFLPCRHLCCCM 240
>At5g18180.1 68418.m02134 Gar1 RNA-binding region family protein
contains Pfam profile PF04410: Gar1 protein RNA binding
region
Length = 189
Score = 28.3 bits (60), Expect = 3.5
Identities = 16/34 (47%), Positives = 18/34 (52%), Gaps = 3/34 (8%)
Frame = +3
Query: 264 QGRPSPGSRG---VPVRDRPPCRGRGRFNFRHSP 356
Q + G RG VP R R P RGRG F R +P
Sbjct: 133 QPKGQSGGRGEGRVPPRGRGPPRGRGNFRGRGAP 166
>At5g41520.1 68418.m05044 40S ribosomal protein S10 (RPS10B)
contains similarity to 40S ribosomal protein S10
Length = 180
Score = 27.1 bits (57), Expect = 8.2
Identities = 17/46 (36%), Positives = 20/46 (43%), Gaps = 3/46 (6%)
Frame = -1
Query: 330 PAPDKADGRELGHPYFPGSD---GPDASTGGRRVPHVDSLRAGCRS 202
PA K + LG P+ G D GP G RR D R G +S
Sbjct: 89 PATLKKQQKPLGRPFGGGGDRPRGPPRGDGERRFGDRDGYRGGPKS 134
>At4g14280.1 68417.m02201 hypothetical protein
Length = 798
Score = 27.1 bits (57), Expect = 8.2
Identities = 18/43 (41%), Positives = 22/43 (51%)
Frame = -2
Query: 293 TPTSRARTALTRLPAGAVYLTLTACELAVAARVTTKLRNSLSF 165
TP R T L +PA LTL A LAV ++ + L SL F
Sbjct: 27 TPEPRRPTQLPPVPAPEKKLTLFALRLAVLEKIASGL-GSLGF 68
>At2g36910.1 68415.m04527 multidrug resistance P-glycoprotein (PGP1)
identical to P-glycoprotein GI:3849833 from [Arabidopsis
thaliana]; homologous to mammalian mdr gene,contains
ATP-binding cassette; related to multi drug resistance
proteins
Length = 1286
Score = 27.1 bits (57), Expect = 8.2
Identities = 15/37 (40%), Positives = 19/37 (51%), Gaps = 1/37 (2%)
Frame = -1
Query: 369 RVSELESDES*TDPAPDKADGR-ELGHPYFPGSDGPD 262
R +E+E D+ T P PD+ G EL H F PD
Sbjct: 1002 RKTEIEPDDPDTTPVPDRLRGEVELKHIDFSYPSRPD 1038
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,707,039
Number of Sequences: 28952
Number of extensions: 182305
Number of successful extensions: 423
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 416
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 423
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1023490624
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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