Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fmgV12a22f
(649 letters)
Database: nematostella
59,808 sequences; 16,821,457 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SB_43527| Best HMM Match : Trypsin (HMM E-Value=0) 42 4e-04
SB_42238| Best HMM Match : Trypsin (HMM E-Value=0) 41 8e-04
SB_4778| Best HMM Match : No HMM Matches (HMM E-Value=.) 39 0.003
SB_52206| Best HMM Match : No HMM Matches (HMM E-Value=.) 39 0.004
SB_33085| Best HMM Match : Trypsin (HMM E-Value=0) 38 0.009
SB_56478| Best HMM Match : Trypsin (HMM E-Value=0) 35 0.050
SB_35182| Best HMM Match : CUB (HMM E-Value=0) 35 0.050
SB_13096| Best HMM Match : No HMM Matches (HMM E-Value=.) 35 0.066
SB_59555| Best HMM Match : No HMM Matches (HMM E-Value=.) 34 0.11
SB_51536| Best HMM Match : Trypsin (HMM E-Value=0) 33 0.20
SB_11023| Best HMM Match : No HMM Matches (HMM E-Value=.) 33 0.20
SB_15907| Best HMM Match : Aldedh (HMM E-Value=3.2e-23) 33 0.26
SB_50354| Best HMM Match : Trypsin (HMM E-Value=0.0028) 33 0.26
SB_42973| Best HMM Match : No HMM Matches (HMM E-Value=.) 31 0.81
SB_26443| Best HMM Match : Trypsin (HMM E-Value=3e-22) 30 1.4
SB_38546| Best HMM Match : Trypsin (HMM E-Value=1.90577e-43) 29 3.3
SB_4906| Best HMM Match : Trypsin (HMM E-Value=0) 29 3.3
SB_48258| Best HMM Match : Kelch_1 (HMM E-Value=0) 28 7.5
SB_43991| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 7.5
SB_35220| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 7.5
SB_22064| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 7.5
SB_36971| Best HMM Match : No HMM Matches (HMM E-Value=.) 27 9.9
>SB_43527| Best HMM Match : Trypsin (HMM E-Value=0)
Length = 366
Score = 41.9 bits (94), Expect = 4e-04
Identities = 23/100 (23%), Positives = 41/100 (41%), Gaps = 3/100 (3%)
Frame = +2
Query: 338 HPEFSEENYDKDVSIVRVTHAIHFGPNXX---XXXXXXXXXXXXXXXXXDLLGWGTTVQG 508
HP +S ++YD D++++R+ + F + GWG
Sbjct: 199 HPHYSPDSYDSDIALIRLAQPVTFTDYVKPICLPSAASDYAQLQANVSGTISGWGKRKLW 258
Query: 509 GSVSDGNLHKLELIVTNKENCREQYKGHDRVVTDNKFCAG 628
LH+ + + + + CR+ + D +VT N FCAG
Sbjct: 259 RDRVANRLHEATVPIVDIQTCRKAHP--DYIVTANMFCAG 296
>SB_42238| Best HMM Match : Trypsin (HMM E-Value=0)
Length = 657
Score = 41.1 bits (92), Expect = 8e-04
Identities = 38/174 (21%), Positives = 66/174 (37%), Gaps = 8/174 (4%)
Frame = +2
Query: 146 EVFLPILNQWFQQCAGIVLTNYHYLSTATCF--HGEFYDPAYRRIIAGSSRRSE------ 301
E+ L N C G ++ Y L+++ CF + R+ RR E
Sbjct: 2 EIILKTPNLTTHYCGGSLIDPYWILTSSHCFWTYNNISTQFEIRLGEHDERRIEGYEEII 61
Query: 302 PGEISYVHFAVNHPEFSEENYDKDVSIVRVTHAIHFGPNXXXXXXXXXXXXXXXXXXXDL 481
G+ Y+H + + D DV+++++ F +
Sbjct: 62 QGDQLYIHPGLVVGDLISPG-DYDVALIKLKRPAVFHKRVHSVCLPSVTANLTTGTKCFV 120
Query: 482 LGWGTTVQGGSVSDGNLHKLELIVTNKENCREQYKGHDRVVTDNKFCAGLVRAG 643
GWG T +G S L++LE+ + +KE C ++ + D FCAG + G
Sbjct: 121 TGWGKTAEGSPYSP-VLNELEVDIVSKEVCNAN-DSYNGTINDRYFCAGFPQGG 172
>SB_4778| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 867
Score = 39.1 bits (87), Expect = 0.003
Identities = 34/166 (20%), Positives = 66/166 (39%), Gaps = 5/166 (3%)
Frame = +2
Query: 161 ILNQWFQQCAGIVLTNYHYLSTATCFHGEFYDPAYRRIIAGSSRRSEPGEISYVHFAV-- 334
I N Q C G ++T ++ A C + +PA ++ G+ R+ P + ++
Sbjct: 649 IYNSGRQFCGGTLVTPEWVITAAHCVVDK--NPASIQVRLGAQDRTSPDPSVEMRISIRS 706
Query: 335 --NHPEF-SEENYDKDVSIVRVTHAIHFGPNXXXXXXXXXXXXXXXXXXXDLLGWGTTVQ 505
NHP++ S + D++++R++ + GWGT
Sbjct: 707 IHNHPDYGSPKRSSNDIALLRLSRPTILTHRINLACMPNDTVHFPNGTMCYITGWGTLSS 766
Query: 506 GGSVSDGNLHKLELIVTNKENCREQYKGHDRVVTDNKFCAGLVRAG 643
GGS + L++ + + + C Y G ++ + CAG G
Sbjct: 767 GGSQPEA-LNQAVVPLRTRSECERSYPGK---ISADMICAGNPEGG 808
>SB_52206| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 786
Score = 38.7 bits (86), Expect = 0.004
Identities = 33/151 (21%), Positives = 58/151 (38%), Gaps = 3/151 (1%)
Frame = +2
Query: 185 CAGIVLTNYHYLSTATCFHGEFYDPAYRRIIAGSSRRSEPG---EISYVHFAVNHPEFSE 355
C G V++ + L+ A C E + ++ G R ++ V ++H +S
Sbjct: 574 CGGNVISPWWVLTAAHCVQDE--RASNIKLTMGEWRLFNVDGTEQVIPVERIISHANYSY 631
Query: 356 ENYDKDVSIVRVTHAIHFGPNXXXXXXXXXXXXXXXXXXXDLLGWGTTVQGGSVSDGNLH 535
D D +++++T ++F + GWG+T GS S L
Sbjct: 632 NTVDYDYALLKLTRPLNF--TQYVQPVCLPDSDFPAGTLCYVTGWGSTNYRGSPSPNYLQ 689
Query: 536 KLELIVTNKENCREQYKGHDRVVTDNKFCAG 628
++ L + N C Y R +T CAG
Sbjct: 690 EVGLPLVNHSQCHATYLTASRKITPRMRCAG 720
>SB_33085| Best HMM Match : Trypsin (HMM E-Value=0)
Length = 537
Score = 37.5 bits (83), Expect = 0.009
Identities = 23/99 (23%), Positives = 39/99 (39%)
Frame = +2
Query: 332 VNHPEFSEENYDKDVSIVRVTHAIHFGPNXXXXXXXXXXXXXXXXXXXDLLGWGTTVQGG 511
++H +S D D +++++T ++F + GWG+T G
Sbjct: 375 ISHANYSYNTVDYDYALLKLTRPLNF--TQYVQPVCLPDSDFPAGTLCYVTGWGSTNYRG 432
Query: 512 SVSDGNLHKLELIVTNKENCREQYKGHDRVVTDNKFCAG 628
S S L ++ L + N C Y R +T CAG
Sbjct: 433 SPSPNYLQEVGLPLVNHSQCHATYLTASRKITPRMRCAG 471
>SB_56478| Best HMM Match : Trypsin (HMM E-Value=0)
Length = 968
Score = 35.1 bits (77), Expect = 0.050
Identities = 28/154 (18%), Positives = 60/154 (38%), Gaps = 5/154 (3%)
Frame = +2
Query: 185 CAGIVLTNYHYLSTATCFHGEFYDPAYRRIIAGSSRRSEPGEISYVHFA---VNHPEFSE 355
C G +++ ++ A C G + P+Y +++ G R+ P H + HP ++
Sbjct: 93 CGGSLVSPTWVVTAAHCIAGSSHTPSY-KVVTGEHIRNSPEGTEQTHDVKRIITHPTYNS 151
Query: 356 ENYDKDVSIVRVTHAIHFGPNXXXXXXXXXXXXXXXXXXXDLLGWGTTVQGGSVSDGNLH 535
D++++ ++ + + GWG G S L
Sbjct: 152 PQLSNDIALIELSSPVPLSDRGHQVSVGSKCF---------ITGWGKIRHPGG-SHHILQ 201
Query: 536 KLELIVTNKENCRE--QYKGHDRVVTDNKFCAGL 631
+ + +++ C++ Q G +TD+ CAG+
Sbjct: 202 QAMMPPLSQDACKKKVQQAGFGIQITDSMVCAGV 235
>SB_35182| Best HMM Match : CUB (HMM E-Value=0)
Length = 963
Score = 35.1 bits (77), Expect = 0.050
Identities = 17/54 (31%), Positives = 29/54 (53%)
Frame = +2
Query: 485 GWGTTVQGGSVSDGNLHKLELIVTNKENCREQYKGHDRVVTDNKFCAGLVRAGG 646
GWG +G + L + ++ + +++ C Q DR +T+N CAG+ R GG
Sbjct: 351 GWGALQEGAGSTSKVLMQAKVPLVSRDQCSHQQSYGDR-ITENMLCAGM-RQGG 402
>SB_13096| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1465
Score = 34.7 bits (76), Expect = 0.066
Identities = 24/100 (24%), Positives = 37/100 (37%), Gaps = 2/100 (2%)
Frame = +2
Query: 335 NHPEFSEENYDKDVSIVRVTHAIHFGPNXXXXXXXXXXXXXXXXXXXD--LLGWGTTVQG 508
+HP FS YD D++++R+ + +LGWG T
Sbjct: 1249 SHPRFSWRTYDSDIALIRLRWKANITEYVRPVCLPNGYQRRLVKPRAKGVMLGWGVTEN- 1307
Query: 509 GSVSDGNLHKLELIVTNKENCREQYKGHDRVVTDNKFCAG 628
L + + V N C++ Y+ VT N CAG
Sbjct: 1308 -KRPSKTLKEAIVPVVNHNACKKAYENETWPVTSNMLCAG 1346
>SB_59555| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 898
Score = 33.9 bits (74), Expect = 0.11
Identities = 30/156 (19%), Positives = 56/156 (35%), Gaps = 2/156 (1%)
Frame = +2
Query: 185 CAGIVLTNYHYLSTATCFHGEFYDPAYRRIIAGSSRRSE-PGEISY-VHFAVNHPEFSEE 358
C G ++ ++ A C Y ++ R P + ++ + H +FS
Sbjct: 57 CGGSLIAKDWVVTAAHCVDRNPTPSGYTVVVGAHHRTGNTPVQNTFRLKQLFKHEQFSMR 116
Query: 359 NYDKDVSIVRVTHAIHFGPNXXXXXXXXXXXXXXXXXXXDLLGWGTTVQGGSVSDGNLHK 538
+ D++++++ + + GWG TV GGS + G L +
Sbjct: 117 HLKNDIALLQLHEPVKASDKVNTVCLPSSGSRAQAGARCYITGWGRTVGGGSAA-GILQQ 175
Query: 539 LELIVTNKENCREQYKGHDRVVTDNKFCAGLVRAGG 646
L V +C+ V + + CAG GG
Sbjct: 176 AMLPVAGDSDCQRTNGRLVPVDSRSMICAGGQGKGG 211
>SB_51536| Best HMM Match : Trypsin (HMM E-Value=0)
Length = 347
Score = 33.1 bits (72), Expect = 0.20
Identities = 30/157 (19%), Positives = 59/157 (37%), Gaps = 3/157 (1%)
Frame = +2
Query: 185 CAGIVLTNYHYLSTATCFHGEFYDPAYRRIIAGSSRRSEPG--EISYVHFAVNHPEFS-E 355
C G V+ ++ A CF Y + + G + V + HP+++
Sbjct: 135 CGGSVVAPEWIVTAAHCFAYSKDAKDYTIAVGEHDLNATDGYEQRPDVERIILHPKYAPH 194
Query: 356 ENYDKDVSIVRVTHAIHFGPNXXXXXXXXXXXXXXXXXXXDLLGWGTTVQGGSVSDGNLH 535
N+D DV+++++ + + + GWG + G LH
Sbjct: 195 NNHDYDVALIKLASPLQYNDRVRPVCLPSLKEDLEENTQCYISGWGHLQEAGH-GPWVLH 253
Query: 536 KLELIVTNKENCREQYKGHDRVVTDNKFCAGLVRAGG 646
+ + + +++ C++ Y V+ CAG AGG
Sbjct: 254 QAAVPLVSRDTCQKAYNDLHYKVSSRMRCAG-YGAGG 289
>SB_11023| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 669
Score = 33.1 bits (72), Expect = 0.20
Identities = 28/163 (17%), Positives = 60/163 (36%), Gaps = 8/163 (4%)
Frame = +2
Query: 176 FQQCAGIVLTNYHYLSTATCFH-----GEFYDPAYRRIIAGSSRRSEPGEISY---VHFA 331
F C G ++++ ++ A H G +P ++ G+ ++ + +
Sbjct: 452 FHTCGGSLISDRWVVTAAQGIHRNKNPGGRQNPGGYTVVVGAHKKRGSTSVQQSLRLSQI 511
Query: 332 VNHPEFSEENYDKDVSIVRVTHAIHFGPNXXXXXXXXXXXXXXXXXXXDLLGWGTTVQGG 511
+ HP++++ D++++ + + F + GWG
Sbjct: 512 IEHPKYNDRRIVNDIALLELATPVQFDSAGKVGTVCLTDQRPAPGKRCYITGWGAINGDT 571
Query: 512 SVSDGNLHKLELIVTNKENCREQYKGHDRVVTDNKFCAGLVRA 640
S L + L + + ENCR +Y V + CAG R+
Sbjct: 572 QQSPDILQQAMLPIASHENCRRKY---GDVSSTAHLCAGEARS 611
Score = 30.7 bits (66), Expect = 1.1
Identities = 12/80 (15%), Positives = 34/80 (42%), Gaps = 2/80 (2%)
Frame = +2
Query: 176 FQQCAGIVLTNYHYLSTATCFHGEFYDPAYRRIIAGSSRRSEPGEISYVHFA--VNHPEF 349
F C G ++++ ++ A C H Y ++ +R + + + HP++
Sbjct: 129 FHTCGGSLISDRWVVTAAHCIHRNKNPGGYTVVVGAHKKRGSTSVQQSLRLSQIIEHPKY 188
Query: 350 SEENYDKDVSIVRVTHAIHF 409
++ D++++ + + F
Sbjct: 189 NDRRIVNDIALLELATPVQF 208
>SB_15907| Best HMM Match : Aldedh (HMM E-Value=3.2e-23)
Length = 275
Score = 32.7 bits (71), Expect = 0.26
Identities = 18/56 (32%), Positives = 30/56 (53%), Gaps = 1/56 (1%)
Frame = -1
Query: 340 MVNSK-VNIRYFTGLTATGRSSDNATVCRIVEFSMETGSS*KVVVVGENNTSALLE 176
M+NS+ +N FTG TGR AT +V+ +E GS ++V+ + + +E
Sbjct: 215 MINSRGINALTFTGSLETGRKVAAATAVNLVKCQLEMGSKNALIVLDDADLDNAVE 270
>SB_50354| Best HMM Match : Trypsin (HMM E-Value=0.0028)
Length = 333
Score = 32.7 bits (71), Expect = 0.26
Identities = 20/66 (30%), Positives = 32/66 (48%), Gaps = 3/66 (4%)
Frame = +2
Query: 146 EVFLPILNQW--FQQCAGIVLTNYHYLSTATCFH-GEFYDPAYRRIIAGSSRRSEPGEIS 316
E F+ + W QC+G ++ H ++ A C H G+++ I+ G S S E+
Sbjct: 138 EPFVSSVKLWSDVMQCSGTLIGPCHVITAAHCIHNGKWFVSPLTAILVGVSNTSSTDELD 197
Query: 317 YVHFAV 334
Y FAV
Sbjct: 198 Y--FAV 201
>SB_42973| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1864
Score = 31.1 bits (67), Expect = 0.81
Identities = 20/91 (21%), Positives = 41/91 (45%), Gaps = 3/91 (3%)
Frame = +2
Query: 146 EVFLPILNQWFQQCAGIVLTNYHYLSTATCFHGEFYDPAYRRIIAGSS---RRSEPGEIS 316
+V L + Q+C G ++ L+ A CF F P + G S RR G+
Sbjct: 1253 QVALTLSGNPVQRCGGALIAADWVLTAAHCF-DRFSMPPEWTVQVGVSDMRRRDGKGQSL 1311
Query: 317 YVHFAVNHPEFSEENYDKDVSIVRVTHAIHF 409
+ HP+++ +++ D++++++ F
Sbjct: 1312 SIKAIFVHPQYNSSSHEHDLAVLQLQQPAEF 1342
>SB_26443| Best HMM Match : Trypsin (HMM E-Value=3e-22)
Length = 231
Score = 30.3 bits (65), Expect = 1.4
Identities = 19/56 (33%), Positives = 28/56 (50%)
Frame = +2
Query: 479 LLGWGTTVQGGSVSDGNLHKLELIVTNKENCREQYKGHDRVVTDNKFCAGLVRAGG 646
++GWG TV GG ++ L + L V +C ++ RV + CAG RA G
Sbjct: 92 VVGWGRTVGGGQGAE-ILQQAMLPVAYDNHCMQRNNQLMRVDPVSMICAGGQRAAG 146
>SB_38546| Best HMM Match : Trypsin (HMM E-Value=1.90577e-43)
Length = 506
Score = 29.1 bits (62), Expect = 3.3
Identities = 17/56 (30%), Positives = 26/56 (46%)
Frame = +2
Query: 479 LLGWGTTVQGGSVSDGNLHKLELIVTNKENCREQYKGHDRVVTDNKFCAGLVRAGG 646
+ GWGT GG+ L + + + ++ C Y + D+ CAGL AGG
Sbjct: 78 ITGWGTLASGGN-QPNKLMQASVPLVSQSKCNTAYPNK---IHDSMLCAGL-DAGG 128
>SB_4906| Best HMM Match : Trypsin (HMM E-Value=0)
Length = 530
Score = 29.1 bits (62), Expect = 3.3
Identities = 17/56 (30%), Positives = 26/56 (46%)
Frame = +2
Query: 479 LLGWGTTVQGGSVSDGNLHKLELIVTNKENCREQYKGHDRVVTDNKFCAGLVRAGG 646
+ GWGT GG+ L + + + ++ C Y + D+ CAGL AGG
Sbjct: 422 ITGWGTLASGGN-QPNKLMQASVPLVSQSKCNTAYPNK---IHDSMLCAGL-DAGG 472
>SB_48258| Best HMM Match : Kelch_1 (HMM E-Value=0)
Length = 473
Score = 27.9 bits (59), Expect = 7.5
Identities = 14/41 (34%), Positives = 22/41 (53%)
Frame = +2
Query: 131 SLVQIEVFLPILNQWFQQCAGIVLTNYHYLSTATCFHGEFY 253
+LV +E + P N+W ++ +LT Y STA G+ Y
Sbjct: 111 NLVSMERYDPSTNEWEEEAVAPMLTARKYFSTAV-LDGKLY 150
>SB_43991| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1149
Score = 27.9 bits (59), Expect = 7.5
Identities = 18/71 (25%), Positives = 33/71 (46%), Gaps = 1/71 (1%)
Frame = -1
Query: 535 VEVAVRYTAALNGSSPSEQI-NKNTLGYYDTLLDNSTLLDVWAEVDGMCYSYDAHILVVI 359
+++ V Y AA+ S Q N+N L Y + D + A + M SY+ + +
Sbjct: 303 MKIKVNYVAAVQDESTYSQFYNENNLKYEEEFTDVLENIKTRARIVIMVTSYNMAVEGMH 362
Query: 358 LLREFGMVNSK 326
L + G++N +
Sbjct: 363 LAKNRGLINGE 373
>SB_35220| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 166
Score = 27.9 bits (59), Expect = 7.5
Identities = 20/77 (25%), Positives = 37/77 (48%)
Frame = -1
Query: 592 MTLVLFPAVFFVGHNEFELVEVAVRYTAALNGSSPSEQINKNTLGYYDTLLDNSTLLDVW 413
M +VLF A+F + EF ++ + + T + +GS + +N Y D +D T+L
Sbjct: 1 MAVVLF-ALFRL--LEFLMLVIFTKGTCSNSGSQQALHVNITCEKYLDVYVDGETMLTGV 57
Query: 412 AEVDGMCYSYDAHILVV 362
V + +H++ V
Sbjct: 58 QGVHSILIDSSSHVMAV 74
>SB_22064| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 398
Score = 27.9 bits (59), Expect = 7.5
Identities = 15/51 (29%), Positives = 25/51 (49%)
Frame = +3
Query: 201 SPTTTTFQLLPVSMENSTILHTVALSLDLPVAVSPVKYLMFTLLLTIPNSL 353
SP+T+ Q++P + + L T + S +LPV + FT IP +
Sbjct: 143 SPSTSVKQMVPNTQASRLCLQTTSASSNLPVTTQQSFRMTFTPPSMIPKPI 193
>SB_36971| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1870
Score = 27.5 bits (58), Expect = 9.9
Identities = 25/117 (21%), Positives = 43/117 (36%), Gaps = 4/117 (3%)
Frame = +2
Query: 290 RRSEPGEISY-VHFAVNHPEFSEENYDKDVSIVRVTHAIHFGPNXXXXXXXXXXXXXXXX 466
RR + E + V + HP + + D DV+++R++H
Sbjct: 1394 RRGDGTEQDFTVRQVIVHPNYRRQTTDSDVALLRLSHPATLNKAVSLICLPKEGESEAVG 1453
Query: 467 XXXDLLGWGTT---VQGGSVSDGNLHKLELIVTNKENCREQYKGHDRVVTDNKFCAG 628
+ GWG T G SV L + + + +++ C + VVT C G
Sbjct: 1454 KNCYITGWGRTGYNRPGASV----LQEAMMPIVSQQTCAAAMRRWG-VVTSRMICGG 1505
Database: nematostella
Posted date: Oct 22, 2007 1:22 PM
Number of letters in database: 16,821,457
Number of sequences in database: 59,808
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 22,293,893
Number of Sequences: 59808
Number of extensions: 516489
Number of successful extensions: 1276
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 1145
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1274
length of database: 16,821,457
effective HSP length: 79
effective length of database: 12,096,625
effective search space used: 1645141000
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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