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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fmgV12a20r
         (721 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_26408| Best HMM Match : Lipase (HMM E-Value=0)                     109   3e-24
SB_19823| Best HMM Match : No HMM Matches (HMM E-Value=.)              95   4e-20
SB_43441| Best HMM Match : Lipase (HMM E-Value=8.19998e-41)            86   2e-17
SB_7713| Best HMM Match : Lipase (HMM E-Value=0.0064)                  60   2e-09
SB_43904| Best HMM Match : Lipase (HMM E-Value=5e-07)                  52   5e-07
SB_4617| Best HMM Match : Extensin_2 (HMM E-Value=0.38)                29   0.56 
SB_5209| Best HMM Match : No HMM Matches (HMM E-Value=.)               30   2.2  
SB_19258| Best HMM Match : No HMM Matches (HMM E-Value=.)              30   2.2  
SB_58542| Best HMM Match : No HMM Matches (HMM E-Value=.)              29   2.9  
SB_6080| Best HMM Match : No HMM Matches (HMM E-Value=.)               29   2.9  
SB_27171| Best HMM Match : No HMM Matches (HMM E-Value=.)              29   5.0  
SB_22961| Best HMM Match : Abhydrolase_1 (HMM E-Value=0.043)           29   5.0  
SB_3986| Best HMM Match : C2 (HMM E-Value=2.9e-31)                     28   6.6  

>SB_26408| Best HMM Match : Lipase (HMM E-Value=0)
          Length = 714

 Score =  109 bits (261), Expect = 3e-24
 Identities = 71/198 (35%), Positives = 102/198 (51%), Gaps = 19/198 (9%)
 Frame = -2

Query: 705 VKDAFLTSGDYNXIVVDWSSFSLSTYSTAVMAVTGVGSSIATFLKNLK----LPLNKVHI 538
           +K+  L  GD+N I+VDW   +   ++ AV     VG+  A  L+ L+      L  VH+
Sbjct: 134 MKNELLWEGDFNVIIVDWMRGAWFPFTRAVANTRLVGAQTARLLQILEERSGRKLAYVHV 193

Query: 537 VGFNLGAHVAGVTGRNLEGK---VARITGLDPSARDW---ENNVLRLGTNDAQYVEVIHT 376
           +GF+ GAHVAG  GR ++ +   + RIT LDP+A  W    +  +RL T+DA +V+VIHT
Sbjct: 194 IGFSFGAHVAGYVGRRMKKRGRMIDRITALDPAAM-WFHKHHEDVRLDTSDALFVDVIHT 252

Query: 375 DGSGVNKNGLGVAIGHIDFFVNGRLVQPGCTN--------NLCSHNRAYEVFAATI-THG 223
                   G+   IGH DF+ NG   QPGC N          C H RA  +F  ++ T  
Sbjct: 253 SAD----YGITSTIGHADFYPNGGKKQPGCDNFFRGFSSYLFCGHKRAPALFTTSLYTKT 308

Query: 222 KHYGNQCSTEAEITANNC 169
             Y   C +E +  + NC
Sbjct: 309 PLYSYPCRSEDDFNSGNC 326


>SB_19823| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 940

 Score = 95.5 bits (227), Expect = 4e-20
 Identities = 63/176 (35%), Positives = 92/176 (52%), Gaps = 17/176 (9%)
 Frame = -2

Query: 705  VKDAFLTSGDYNXIVVDWSSFSLSTYSTAVMAVTGVGSSIATFLKNLKLPLNKV----HI 538
            ++ A +   D N I  DWS  +   Y  A      VG+ I   +K L           ++
Sbjct: 632  IRHALIKQEDANVITTDWSRGATIPYEQATANTRMVGAQITELIKFLNNQTGNTPASFYL 691

Query: 537  VGFNLGAHVAGVTGRNLEG---KVARITGLDPSARDWEN-NV-LRLGTNDAQYVEVIHTD 373
            VGF+LGAH++G  GR +     K+ RITGLDP++  + N +V +RL  +DA +V+V+HTD
Sbjct: 692  VGFSLGAHISGYVGRRIAKTGQKLNRITGLDPASIHFVNAHVDVRLDPSDADFVDVMHTD 751

Query: 372  GSGVNKNGLGVAIGHIDFFVNGRLVQPGCTNNL--------CSHNRAYEVFAATIT 229
               ++  G     GHIDF+ NG   QPGC + L        C H RA E +A ++T
Sbjct: 752  ---MDLAGTPTVSGHIDFYPNGGKKQPGCRDLLDGPINYVICDHMRAPEYYAESVT 804


>SB_43441| Best HMM Match : Lipase (HMM E-Value=8.19998e-41)
          Length = 291

 Score = 86.2 bits (204), Expect = 2e-17
 Identities = 57/151 (37%), Positives = 83/151 (54%), Gaps = 10/151 (6%)
 Frame = -2

Query: 705 VKDAFLTSGDYNXIVVDWSSFSLSTYSTAVMAVTGVGSSIATFLKN-LKLPLNK----VH 541
           +KD  L   D N I+VDW   +   Y  AV     VG+ +A F+K  L L  ++     H
Sbjct: 115 MKDELLRKSDDNVIIVDWIRGAKIPYVRAVANTRLVGAQVAAFMKTILSLSGSREGGAFH 174

Query: 540 IVGFNLGAHVAGVTGRNLE--GK-VARITGLDPSARDWENNV--LRLGTNDAQYVEVIHT 376
            +GF+LGAH++G  G+ L+  G+ + RITGLDP+   ++     +RL   DAQ+V+VIHT
Sbjct: 175 SIGFSLGAHISGYVGQRLKRIGRHLDRITGLDPATLMFKGEAPDVRLDRLDAQFVDVIHT 234

Query: 375 DGSGVNKNGLGVAIGHIDFFVNGRLVQPGCT 283
                   G+    GH+DF+ NG   Q GC+
Sbjct: 235 S----YVFGITAPHGHMDFYPNGGTSQRGCS 261


>SB_7713| Best HMM Match : Lipase (HMM E-Value=0.0064)
          Length = 131

 Score = 59.7 bits (138), Expect = 2e-09
 Identities = 33/88 (37%), Positives = 47/88 (53%), Gaps = 6/88 (6%)
 Frame = -2

Query: 708 IVKDAFLTSGDYNXIVVDWSSFS--LSTYSTAVMAVTGVGSSIATFLKNLK----LPLNK 547
           +++D  L     N I VDW S +  L+ Y  A      VG+ +A  +  ++      L +
Sbjct: 43  VMRDELLKREPMNVITVDWQSGADGLNLYHVAAGNTRVVGAQLAELITTIQRVFDFDLRR 102

Query: 546 VHIVGFNLGAHVAGVTGRNLEGKVARIT 463
           VH++G +LGAHVAG  G  L GKV RIT
Sbjct: 103 VHLIGHSLGAHVAGYAGERLSGKVGRIT 130


>SB_43904| Best HMM Match : Lipase (HMM E-Value=5e-07)
          Length = 408

 Score = 52.0 bits (119), Expect = 5e-07
 Identities = 34/87 (39%), Positives = 49/87 (56%), Gaps = 10/87 (11%)
 Frame = -2

Query: 459 LDPSARDWENNVL--RLGTNDAQYVEVIHTDGSGVNKNGLGVAIGHIDFFVNGRLVQPGC 286
           LDP+   + N  +  RL  +DA++V+V+HTD   ++  G     GHIDF+ NG   QPGC
Sbjct: 2   LDPAGLYFVNEHVDVRLDPSDAEFVDVMHTD---MDFAGTSTQSGHIDFYPNGGKNQPGC 58

Query: 285 -------TNNL-CSHNRAYEVFAATIT 229
                  +N L C H RA++ F  +IT
Sbjct: 59  RDIADGPSNALKCDHVRAHDYFTESIT 85


>SB_4617| Best HMM Match : Extensin_2 (HMM E-Value=0.38)
          Length = 450

 Score = 29.5 bits (63), Expect(2) = 0.56
 Identities = 17/59 (28%), Positives = 28/59 (47%), Gaps = 1/59 (1%)
 Frame = +2

Query: 290 PGWTRRPLTKKSMC-PIATPKPFLLTPDPSV*ITSTY*ASFVPSRSTLFSQSRAEGSRP 463
           P    +P+    +C P+ +P P +++P P   I  +   + VP+  TL  QS A    P
Sbjct: 193 PSPASQPVAPSPVCIPVQSPAPNMMSPSPQQFIAQSPAPTSVPTPGTLNVQSPASILNP 251



 Score = 21.0 bits (42), Expect(2) = 0.56
 Identities = 7/21 (33%), Positives = 13/21 (61%)
 Frame = +2

Query: 584 AILDPTPVTAITAVEYVDKLN 646
           ++  P P++A     Y++KLN
Sbjct: 257 SVTSPAPLSAAEEQAYLEKLN 277


>SB_5209| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 67

 Score = 29.9 bits (64), Expect = 2.2
 Identities = 15/31 (48%), Positives = 21/31 (67%), Gaps = 4/31 (12%)
 Frame = -2

Query: 540 IVGFNLGAHVAGVTG---RNLEG-KVARITG 460
           ++GF+LG HVAG  G   +N  G K+ RI+G
Sbjct: 21  VIGFSLGGHVAGYAGSRIKNTTGLKLGRISG 51


>SB_19258| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 459

 Score = 29.9 bits (64), Expect = 2.2
 Identities = 23/90 (25%), Positives = 37/90 (41%), Gaps = 3/90 (3%)
 Frame = -2

Query: 423 LRLGTNDAQYVEVIHTDGSGVNKNGLGVAI-GHID-FFVNGRLVQPGCTNNLCSHNRAYE 250
           + + +++  ++   +   SG   +     +  HID   +NG +V PG    +      Y 
Sbjct: 1   MSMSSDEVNFLVYRYLQESGFQHSAFTFGVESHIDQSSINGSVVPPGALVTILQKGVQYV 60

Query: 249 VFAATITHGKHYGNQCSTEAEITANN-CRG 163
              AT+T G  Y      EAE T    CRG
Sbjct: 61  EAEATVTEGVQY-----VEAEATVTEVCRG 85


>SB_58542| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 268

 Score = 29.5 bits (63), Expect = 2.9
 Identities = 12/47 (25%), Positives = 24/47 (51%)
 Frame = -2

Query: 588 IATFLKNLKLPLNKVHIVGFNLGAHVAGVTGRNLEGKVARITGLDPS 448
           + +FL  + +   K H+ G ++G H+ GV       +VA +  + P+
Sbjct: 120 LLSFLHAVGVNKRKFHLAGISMGGHIVGVYAAQHPARVASVIMMCPA 166


>SB_6080| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 2101

 Score = 29.5 bits (63), Expect = 2.9
 Identities = 14/45 (31%), Positives = 20/45 (44%)
 Frame = -2

Query: 324  DFFVNGRLVQPGCTNNLCSHNRAYEVFAATITHGKHYGNQCSTEA 190
            D++   ++   G T+  C     YE      T GK YGN+C   A
Sbjct: 1278 DYYAQCQVEDDGSTSCKCPIFCTYEYMPVCGTDGKTYGNKCEMRA 1322


>SB_27171| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 52

 Score = 28.7 bits (61), Expect = 5.0
 Identities = 15/37 (40%), Positives = 20/37 (54%), Gaps = 3/37 (8%)
 Frame = -2

Query: 357 KNGLGVAIG---HIDFFVNGRLVQPGCTNNLCSHNRA 256
           K+  G+ IG   H+D  VNG  VQ       C+HN+A
Sbjct: 12  KSSEGMYIGADLHLDKDVNGAYVQGSFIGGFCTHNKA 48


>SB_22961| Best HMM Match : Abhydrolase_1 (HMM E-Value=0.043)
          Length = 183

 Score = 28.7 bits (61), Expect = 5.0
 Identities = 12/44 (27%), Positives = 22/44 (50%)
 Frame = -2

Query: 579 FLKNLKLPLNKVHIVGFNLGAHVAGVTGRNLEGKVARITGLDPS 448
           FL  + +   K H+ G ++G H+ GV       +VA +  + P+
Sbjct: 1   FLHAVGVNKRKFHLAGISMGGHIVGVYAAQHPARVASVIMMCPA 44


>SB_3986| Best HMM Match : C2 (HMM E-Value=2.9e-31)
          Length = 778

 Score = 28.3 bits (60), Expect = 6.6
 Identities = 12/23 (52%), Positives = 14/23 (60%)
 Frame = +2

Query: 14  VETNFIGHHRKLRFKFCRHHKWN 82
           VE NF+GH R+ R   CR  K N
Sbjct: 233 VERNFVGHRRRCRRVVCRATKSN 255


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 22,305,719
Number of Sequences: 59808
Number of extensions: 481757
Number of successful extensions: 1120
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 1017
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1110
length of database: 16,821,457
effective HSP length: 80
effective length of database: 12,036,817
effective search space used: 1913853903
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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