BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fmgV12a20r (721 letters) Database: nematostella 59,808 sequences; 16,821,457 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SB_26408| Best HMM Match : Lipase (HMM E-Value=0) 109 3e-24 SB_19823| Best HMM Match : No HMM Matches (HMM E-Value=.) 95 4e-20 SB_43441| Best HMM Match : Lipase (HMM E-Value=8.19998e-41) 86 2e-17 SB_7713| Best HMM Match : Lipase (HMM E-Value=0.0064) 60 2e-09 SB_43904| Best HMM Match : Lipase (HMM E-Value=5e-07) 52 5e-07 SB_4617| Best HMM Match : Extensin_2 (HMM E-Value=0.38) 29 0.56 SB_5209| Best HMM Match : No HMM Matches (HMM E-Value=.) 30 2.2 SB_19258| Best HMM Match : No HMM Matches (HMM E-Value=.) 30 2.2 SB_58542| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 2.9 SB_6080| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 2.9 SB_27171| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 5.0 SB_22961| Best HMM Match : Abhydrolase_1 (HMM E-Value=0.043) 29 5.0 SB_3986| Best HMM Match : C2 (HMM E-Value=2.9e-31) 28 6.6 >SB_26408| Best HMM Match : Lipase (HMM E-Value=0) Length = 714 Score = 109 bits (261), Expect = 3e-24 Identities = 71/198 (35%), Positives = 102/198 (51%), Gaps = 19/198 (9%) Frame = -2 Query: 705 VKDAFLTSGDYNXIVVDWSSFSLSTYSTAVMAVTGVGSSIATFLKNLK----LPLNKVHI 538 +K+ L GD+N I+VDW + ++ AV VG+ A L+ L+ L VH+ Sbjct: 134 MKNELLWEGDFNVIIVDWMRGAWFPFTRAVANTRLVGAQTARLLQILEERSGRKLAYVHV 193 Query: 537 VGFNLGAHVAGVTGRNLEGK---VARITGLDPSARDW---ENNVLRLGTNDAQYVEVIHT 376 +GF+ GAHVAG GR ++ + + RIT LDP+A W + +RL T+DA +V+VIHT Sbjct: 194 IGFSFGAHVAGYVGRRMKKRGRMIDRITALDPAAM-WFHKHHEDVRLDTSDALFVDVIHT 252 Query: 375 DGSGVNKNGLGVAIGHIDFFVNGRLVQPGCTN--------NLCSHNRAYEVFAATI-THG 223 G+ IGH DF+ NG QPGC N C H RA +F ++ T Sbjct: 253 SAD----YGITSTIGHADFYPNGGKKQPGCDNFFRGFSSYLFCGHKRAPALFTTSLYTKT 308 Query: 222 KHYGNQCSTEAEITANNC 169 Y C +E + + NC Sbjct: 309 PLYSYPCRSEDDFNSGNC 326 >SB_19823| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 940 Score = 95.5 bits (227), Expect = 4e-20 Identities = 63/176 (35%), Positives = 92/176 (52%), Gaps = 17/176 (9%) Frame = -2 Query: 705 VKDAFLTSGDYNXIVVDWSSFSLSTYSTAVMAVTGVGSSIATFLKNLKLPLNKV----HI 538 ++ A + D N I DWS + Y A VG+ I +K L ++ Sbjct: 632 IRHALIKQEDANVITTDWSRGATIPYEQATANTRMVGAQITELIKFLNNQTGNTPASFYL 691 Query: 537 VGFNLGAHVAGVTGRNLEG---KVARITGLDPSARDWEN-NV-LRLGTNDAQYVEVIHTD 373 VGF+LGAH++G GR + K+ RITGLDP++ + N +V +RL +DA +V+V+HTD Sbjct: 692 VGFSLGAHISGYVGRRIAKTGQKLNRITGLDPASIHFVNAHVDVRLDPSDADFVDVMHTD 751 Query: 372 GSGVNKNGLGVAIGHIDFFVNGRLVQPGCTNNL--------CSHNRAYEVFAATIT 229 ++ G GHIDF+ NG QPGC + L C H RA E +A ++T Sbjct: 752 ---MDLAGTPTVSGHIDFYPNGGKKQPGCRDLLDGPINYVICDHMRAPEYYAESVT 804 >SB_43441| Best HMM Match : Lipase (HMM E-Value=8.19998e-41) Length = 291 Score = 86.2 bits (204), Expect = 2e-17 Identities = 57/151 (37%), Positives = 83/151 (54%), Gaps = 10/151 (6%) Frame = -2 Query: 705 VKDAFLTSGDYNXIVVDWSSFSLSTYSTAVMAVTGVGSSIATFLKN-LKLPLNK----VH 541 +KD L D N I+VDW + Y AV VG+ +A F+K L L ++ H Sbjct: 115 MKDELLRKSDDNVIIVDWIRGAKIPYVRAVANTRLVGAQVAAFMKTILSLSGSREGGAFH 174 Query: 540 IVGFNLGAHVAGVTGRNLE--GK-VARITGLDPSARDWENNV--LRLGTNDAQYVEVIHT 376 +GF+LGAH++G G+ L+ G+ + RITGLDP+ ++ +RL DAQ+V+VIHT Sbjct: 175 SIGFSLGAHISGYVGQRLKRIGRHLDRITGLDPATLMFKGEAPDVRLDRLDAQFVDVIHT 234 Query: 375 DGSGVNKNGLGVAIGHIDFFVNGRLVQPGCT 283 G+ GH+DF+ NG Q GC+ Sbjct: 235 S----YVFGITAPHGHMDFYPNGGTSQRGCS 261 >SB_7713| Best HMM Match : Lipase (HMM E-Value=0.0064) Length = 131 Score = 59.7 bits (138), Expect = 2e-09 Identities = 33/88 (37%), Positives = 47/88 (53%), Gaps = 6/88 (6%) Frame = -2 Query: 708 IVKDAFLTSGDYNXIVVDWSSFS--LSTYSTAVMAVTGVGSSIATFLKNLK----LPLNK 547 +++D L N I VDW S + L+ Y A VG+ +A + ++ L + Sbjct: 43 VMRDELLKREPMNVITVDWQSGADGLNLYHVAAGNTRVVGAQLAELITTIQRVFDFDLRR 102 Query: 546 VHIVGFNLGAHVAGVTGRNLEGKVARIT 463 VH++G +LGAHVAG G L GKV RIT Sbjct: 103 VHLIGHSLGAHVAGYAGERLSGKVGRIT 130 >SB_43904| Best HMM Match : Lipase (HMM E-Value=5e-07) Length = 408 Score = 52.0 bits (119), Expect = 5e-07 Identities = 34/87 (39%), Positives = 49/87 (56%), Gaps = 10/87 (11%) Frame = -2 Query: 459 LDPSARDWENNVL--RLGTNDAQYVEVIHTDGSGVNKNGLGVAIGHIDFFVNGRLVQPGC 286 LDP+ + N + RL +DA++V+V+HTD ++ G GHIDF+ NG QPGC Sbjct: 2 LDPAGLYFVNEHVDVRLDPSDAEFVDVMHTD---MDFAGTSTQSGHIDFYPNGGKNQPGC 58 Query: 285 -------TNNL-CSHNRAYEVFAATIT 229 +N L C H RA++ F +IT Sbjct: 59 RDIADGPSNALKCDHVRAHDYFTESIT 85 >SB_4617| Best HMM Match : Extensin_2 (HMM E-Value=0.38) Length = 450 Score = 29.5 bits (63), Expect(2) = 0.56 Identities = 17/59 (28%), Positives = 28/59 (47%), Gaps = 1/59 (1%) Frame = +2 Query: 290 PGWTRRPLTKKSMC-PIATPKPFLLTPDPSV*ITSTY*ASFVPSRSTLFSQSRAEGSRP 463 P +P+ +C P+ +P P +++P P I + + VP+ TL QS A P Sbjct: 193 PSPASQPVAPSPVCIPVQSPAPNMMSPSPQQFIAQSPAPTSVPTPGTLNVQSPASILNP 251 Score = 21.0 bits (42), Expect(2) = 0.56 Identities = 7/21 (33%), Positives = 13/21 (61%) Frame = +2 Query: 584 AILDPTPVTAITAVEYVDKLN 646 ++ P P++A Y++KLN Sbjct: 257 SVTSPAPLSAAEEQAYLEKLN 277 >SB_5209| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 67 Score = 29.9 bits (64), Expect = 2.2 Identities = 15/31 (48%), Positives = 21/31 (67%), Gaps = 4/31 (12%) Frame = -2 Query: 540 IVGFNLGAHVAGVTG---RNLEG-KVARITG 460 ++GF+LG HVAG G +N G K+ RI+G Sbjct: 21 VIGFSLGGHVAGYAGSRIKNTTGLKLGRISG 51 >SB_19258| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 459 Score = 29.9 bits (64), Expect = 2.2 Identities = 23/90 (25%), Positives = 37/90 (41%), Gaps = 3/90 (3%) Frame = -2 Query: 423 LRLGTNDAQYVEVIHTDGSGVNKNGLGVAI-GHID-FFVNGRLVQPGCTNNLCSHNRAYE 250 + + +++ ++ + SG + + HID +NG +V PG + Y Sbjct: 1 MSMSSDEVNFLVYRYLQESGFQHSAFTFGVESHIDQSSINGSVVPPGALVTILQKGVQYV 60 Query: 249 VFAATITHGKHYGNQCSTEAEITANN-CRG 163 AT+T G Y EAE T CRG Sbjct: 61 EAEATVTEGVQY-----VEAEATVTEVCRG 85 >SB_58542| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 268 Score = 29.5 bits (63), Expect = 2.9 Identities = 12/47 (25%), Positives = 24/47 (51%) Frame = -2 Query: 588 IATFLKNLKLPLNKVHIVGFNLGAHVAGVTGRNLEGKVARITGLDPS 448 + +FL + + K H+ G ++G H+ GV +VA + + P+ Sbjct: 120 LLSFLHAVGVNKRKFHLAGISMGGHIVGVYAAQHPARVASVIMMCPA 166 >SB_6080| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 2101 Score = 29.5 bits (63), Expect = 2.9 Identities = 14/45 (31%), Positives = 20/45 (44%) Frame = -2 Query: 324 DFFVNGRLVQPGCTNNLCSHNRAYEVFAATITHGKHYGNQCSTEA 190 D++ ++ G T+ C YE T GK YGN+C A Sbjct: 1278 DYYAQCQVEDDGSTSCKCPIFCTYEYMPVCGTDGKTYGNKCEMRA 1322 >SB_27171| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 52 Score = 28.7 bits (61), Expect = 5.0 Identities = 15/37 (40%), Positives = 20/37 (54%), Gaps = 3/37 (8%) Frame = -2 Query: 357 KNGLGVAIG---HIDFFVNGRLVQPGCTNNLCSHNRA 256 K+ G+ IG H+D VNG VQ C+HN+A Sbjct: 12 KSSEGMYIGADLHLDKDVNGAYVQGSFIGGFCTHNKA 48 >SB_22961| Best HMM Match : Abhydrolase_1 (HMM E-Value=0.043) Length = 183 Score = 28.7 bits (61), Expect = 5.0 Identities = 12/44 (27%), Positives = 22/44 (50%) Frame = -2 Query: 579 FLKNLKLPLNKVHIVGFNLGAHVAGVTGRNLEGKVARITGLDPS 448 FL + + K H+ G ++G H+ GV +VA + + P+ Sbjct: 1 FLHAVGVNKRKFHLAGISMGGHIVGVYAAQHPARVASVIMMCPA 44 >SB_3986| Best HMM Match : C2 (HMM E-Value=2.9e-31) Length = 778 Score = 28.3 bits (60), Expect = 6.6 Identities = 12/23 (52%), Positives = 14/23 (60%) Frame = +2 Query: 14 VETNFIGHHRKLRFKFCRHHKWN 82 VE NF+GH R+ R CR K N Sbjct: 233 VERNFVGHRRRCRRVVCRATKSN 255 Database: nematostella Posted date: Oct 22, 2007 1:22 PM Number of letters in database: 16,821,457 Number of sequences in database: 59,808 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 22,305,719 Number of Sequences: 59808 Number of extensions: 481757 Number of successful extensions: 1120 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 1017 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1110 length of database: 16,821,457 effective HSP length: 80 effective length of database: 12,036,817 effective search space used: 1913853903 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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