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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fmgV12a06r
         (663 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_32751| Best HMM Match : Pentapeptide_2 (HMM E-Value=1.8)            31   1.1  
SB_33579| Best HMM Match : Laminin_G_2 (HMM E-Value=3.2e-34)           29   2.6  
SB_33399| Best HMM Match : Ank (HMM E-Value=0)                         29   3.4  
SB_53822| Best HMM Match : No HMM Matches (HMM E-Value=.)              29   4.5  
SB_17765| Best HMM Match : 7tm_1 (HMM E-Value=7.6e-16)                 29   4.5  
SB_30999| Best HMM Match : DUF1233 (HMM E-Value=7.1)                   28   5.9  
SB_48953| Best HMM Match : NIF3 (HMM E-Value=1.7e-12)                  28   5.9  

>SB_32751| Best HMM Match : Pentapeptide_2 (HMM E-Value=1.8)
          Length = 127

 Score = 30.7 bits (66), Expect = 1.1
 Identities = 16/43 (37%), Positives = 25/43 (58%), Gaps = 1/43 (2%)
 Frame = -2

Query: 374 GFKDLGYSFVAGGNGKIYEGAGWNHIGAHTLHYNNISIG-IGF 249
           G+ D+GY+ + G N   Y   G+N IG + + YN+I    IG+
Sbjct: 47  GYNDIGYNDI-GYNDIGYNDIGYNDIGYNDIGYNDIGYNDIGY 88



 Score = 30.7 bits (66), Expect = 1.1
 Identities = 16/43 (37%), Positives = 25/43 (58%), Gaps = 1/43 (2%)
 Frame = -2

Query: 374 GFKDLGYSFVAGGNGKIYEGAGWNHIGAHTLHYNNISIG-IGF 249
           G+ D+GY+ + G N   Y   G+N IG + + YN+I    IG+
Sbjct: 57  GYNDIGYNDI-GYNDIGYNDIGYNDIGYNDIGYNDIGYNDIGY 98



 Score = 30.7 bits (66), Expect = 1.1
 Identities = 16/43 (37%), Positives = 25/43 (58%), Gaps = 1/43 (2%)
 Frame = -2

Query: 374 GFKDLGYSFVAGGNGKIYEGAGWNHIGAHTLHYNNISIG-IGF 249
           G+ D+GY+ + G N   Y   G+N IG + + YN+I    IG+
Sbjct: 67  GYNDIGYNDI-GYNDIGYNDIGYNDIGYNDIGYNDIGYNDIGY 108



 Score = 30.7 bits (66), Expect = 1.1
 Identities = 16/43 (37%), Positives = 25/43 (58%), Gaps = 1/43 (2%)
 Frame = -2

Query: 374 GFKDLGYSFVAGGNGKIYEGAGWNHIGAHTLHYNNISIG-IGF 249
           G+ D+GY+ + G N   Y   G+N IG + + YN+I    IG+
Sbjct: 77  GYNDIGYNDI-GYNDIGYNDIGYNDIGYNDIGYNDIGYNDIGY 118



 Score = 30.3 bits (65), Expect = 1.5
 Identities = 14/36 (38%), Positives = 22/36 (61%)
 Frame = -2

Query: 374 GFKDLGYSFVAGGNGKIYEGAGWNHIGAHTLHYNNI 267
           G+ D+GY+ + G N   Y   G+N IG + + YN+I
Sbjct: 87  GYNDIGYNDI-GYNDIGYNDIGYNDIGYNDIGYNDI 121



 Score = 30.3 bits (65), Expect = 1.5
 Identities = 14/36 (38%), Positives = 22/36 (61%)
 Frame = -2

Query: 374 GFKDLGYSFVAGGNGKIYEGAGWNHIGAHTLHYNNI 267
           G+ D+GY+ + G N   Y   G+N IG + + YN+I
Sbjct: 92  GYNDIGYNDI-GYNDIGYNDIGYNDIGYNDIGYNDI 126



 Score = 28.3 bits (60), Expect = 5.9
 Identities = 19/65 (29%), Positives = 33/65 (50%), Gaps = 5/65 (7%)
 Frame = -2

Query: 428 EECLLSVNSLRQHHMLLAGFKDLGYSFVA----GGNGKIYEGAGWNHIGAHTLHYNNISI 261
           EE  +S+  +   H+ +  + D+GY+ +     G N   Y   G+N IG + + YN+I  
Sbjct: 15  EERYISIGHISIGHISIE-YNDIGYNDIEYNDIGYNDIGYNDIGYNDIGYNDIGYNDIGY 73

Query: 260 G-IGF 249
             IG+
Sbjct: 74  NDIGY 78


>SB_33579| Best HMM Match : Laminin_G_2 (HMM E-Value=3.2e-34)
          Length = 1071

 Score = 29.5 bits (63), Expect = 2.6
 Identities = 13/36 (36%), Positives = 22/36 (61%)
 Frame = -3

Query: 379 WLGSRTWAIHSWLEATEKFMKERDGTISVLTHCTTI 272
           W+ SR+ ++HSW++ T K M + +   +  T C TI
Sbjct: 534 WI-SRSGSLHSWVDGTIKNMDQPESQQTSQTDCDTI 568


>SB_33399| Best HMM Match : Ank (HMM E-Value=0)
          Length = 1416

 Score = 29.1 bits (62), Expect = 3.4
 Identities = 18/48 (37%), Positives = 28/48 (58%), Gaps = 2/48 (4%)
 Frame = +1

Query: 409  TLSKHSSSVKQ--SLDTVCCITTKSIGLFRGCLRRDSVPLHSVMGISP 546
            T+S   SS+ Q  ++D++   + + IG  R C R  + PL S+ GISP
Sbjct: 1245 TISPIGSSLAQCSTVDSMDKPSLRPIGTERACRRATASPLPSMPGISP 1292


>SB_53822| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 258

 Score = 28.7 bits (61), Expect = 4.5
 Identities = 11/33 (33%), Positives = 20/33 (60%)
 Frame = +3

Query: 300 MVPSRSFINFSVASSHE*IAQVLEPSQKHMMLS 398
           + PS +F +F +   HE +  ++ P+ KH+ LS
Sbjct: 31  LYPSINFESFHIPFDHEEVKDLIPPTAKHVFLS 63


>SB_17765| Best HMM Match : 7tm_1 (HMM E-Value=7.6e-16)
          Length = 424

 Score = 28.7 bits (61), Expect = 4.5
 Identities = 13/39 (33%), Positives = 20/39 (51%)
 Frame = -2

Query: 362 LGYSFVAGGNGKIYEGAGWNHIGAHTLHYNNISIGIGFI 246
           +G+S    G  K+  G  W    A TL YN + + +GF+
Sbjct: 159 VGWSKFVPGAAKVSCGPDWETQNASTLSYNIVLLIVGFV 197


>SB_30999| Best HMM Match : DUF1233 (HMM E-Value=7.1)
          Length = 142

 Score = 28.3 bits (60), Expect = 5.9
 Identities = 12/36 (33%), Positives = 22/36 (61%)
 Frame = -3

Query: 379 WLGSRTWAIHSWLEATEKFMKERDGTISVLTHCTTI 272
           W+ SR+ ++HSW++ T K M + +   +  T C T+
Sbjct: 51  WI-SRSGSLHSWVDGTIKNMDQPESQQTSQTDCDTM 85


>SB_48953| Best HMM Match : NIF3 (HMM E-Value=1.7e-12)
          Length = 288

 Score = 28.3 bits (60), Expect = 5.9
 Identities = 15/50 (30%), Positives = 24/50 (48%)
 Frame = -2

Query: 326 IYEGAGWNHIGAHTLHYNNISIGIGFIGDFREKLPTQQALQAVQDFLACG 177
           IYE   +     +  H N   IG+G IG+F   +   + L  V++ + CG
Sbjct: 144 IYEEVAYEIYSLNNKHQN---IGLGMIGEFETPMNETEFLAFVKNKMQCG 190


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 21,621,192
Number of Sequences: 59808
Number of extensions: 457006
Number of successful extensions: 1451
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 1299
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1445
length of database: 16,821,457
effective HSP length: 79
effective length of database: 12,096,625
effective search space used: 1705624125
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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