BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fmgV11p12f (543 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g29120.2 68414.m03565 expressed protein 31 0.66 At1g29120.1 68414.m03564 expressed protein 31 0.66 At2g47510.1 68415.m05930 fumarate hydratase, putative / fumarase... 30 1.1 At3g17950.1 68416.m02285 expressed protein ; expression supporte... 29 1.5 At5g50950.1 68418.m06318 fumarate hydratase, putative / fumarase... 29 2.0 At5g07490.1 68418.m00857 hypothetical protein 29 2.0 At1g78010.1 68414.m09091 tRNA modification GTPase, putative simi... 29 2.0 At5g50950.2 68418.m06319 fumarate hydratase, putative / fumarase... 28 3.5 At3g54220.1 68416.m05993 scarecrow transcription factor, putativ... 28 3.5 At4g33350.1 68417.m04741 chloroplast inner membrane import prote... 27 6.1 At3g01870.1 68416.m00132 hypothetical protein 27 6.1 At3g13570.1 68416.m01707 SC35-like splicing factor, 30a kD (SCL3... 27 8.1 >At1g29120.2 68414.m03565 expressed protein Length = 455 Score = 30.7 bits (66), Expect = 0.66 Identities = 21/46 (45%), Positives = 26/46 (56%), Gaps = 6/46 (13%) Frame = +2 Query: 230 STGSLW--PPAFAYLPSRPGKWGKPRTR----LSGSLVAASASTTS 349 ST S W P F YLP R G + KP +R S +V+ASAS+ S Sbjct: 2 STASSWIQQPPFRYLPDR-GGFSKPSSRSARQFSSGVVSASASSPS 46 >At1g29120.1 68414.m03564 expressed protein Length = 455 Score = 30.7 bits (66), Expect = 0.66 Identities = 21/46 (45%), Positives = 26/46 (56%), Gaps = 6/46 (13%) Frame = +2 Query: 230 STGSLW--PPAFAYLPSRPGKWGKPRTR----LSGSLVAASASTTS 349 ST S W P F YLP R G + KP +R S +V+ASAS+ S Sbjct: 2 STASSWIQQPPFRYLPDR-GGFSKPSSRSARQFSSGVVSASASSPS 46 >At2g47510.1 68415.m05930 fumarate hydratase, putative / fumarase, putative similar to SP|P55250 Fumarate hydratase, mitochondrial precursor (EC 4.2.1.2) (Fumarase) {Rhizopus oryzae}; contains Pfam profile PF00206: Lyase Length = 492 Score = 29.9 bits (64), Expect = 1.1 Identities = 26/93 (27%), Positives = 40/93 (43%), Gaps = 3/93 (3%) Frame = +1 Query: 4 IILKDQKPGSLLQVGKMKFTLLFVLVIVTAVAADEEAGVR---SNGRPDLVIGTITSQDS 174 ++L + +PGS + GK+ T L +V A V SNG +L + + Sbjct: 337 LVLPENEPGSSIMPGKVNPTQCEALTMVCAQVMGNHVAVTVGGSNGHFELNVFKPVIASA 396 Query: 175 LIRSFRFNRPASPSSQSHFHRIAMAAGVRISAI 273 L+ S R AS S + + R A RIS + Sbjct: 397 LLHSVRLIADASASFEKNCVRGIEANRERISKL 429 >At3g17950.1 68416.m02285 expressed protein ; expression supported by MPSS Length = 211 Score = 29.5 bits (63), Expect = 1.5 Identities = 11/26 (42%), Positives = 14/26 (53%) Frame = +3 Query: 252 RRSHICHHGPGSGANPEPDYPGRWWR 329 RR+H P + A PEP +WWR Sbjct: 75 RRNHQRKRPPSNSAEPEPHRRRKWWR 100 >At5g50950.1 68418.m06318 fumarate hydratase, putative / fumarase, putative similar to SP|P55250 Fumarate hydratase, mitochondrial precursor (EC 4.2.1.2) (Fumarase) {Rhizopus oryzae}; contains Pfam profile PF00206: Lyase Length = 510 Score = 29.1 bits (62), Expect = 2.0 Identities = 33/132 (25%), Positives = 51/132 (38%), Gaps = 3/132 (2%) Frame = +1 Query: 10 LKDQKPGSLLQVGKMKFTLLFVLVIVTAVAADEEAGVR---SNGRPDLVIGTITSQDSLI 180 L + +PGS + GK+ T L +V A V SNG +L + +L+ Sbjct: 346 LPENEPGSSIMPGKVNPTQCEALTMVCAQVMGNHVAVTIGGSNGHFELNVFKPVIASALL 405 Query: 181 RSFRFNRPASPSSQSHFHRIAMAAGVRISAITAREVGQTQNPTIRVAGGGIGFNNVNLEA 360 S R AS S + + R A RIS + + + IG++N A Sbjct: 406 HSIRLIADASASFEKNCVRGIEANRERISKLLHESL-----MLVTSLNPKIGYDNAAAVA 460 Query: 361 RNARGRGFSYNV 396 + A G + V Sbjct: 461 KRAHKEGCTLKV 472 >At5g07490.1 68418.m00857 hypothetical protein Length = 145 Score = 29.1 bits (62), Expect = 2.0 Identities = 12/24 (50%), Positives = 18/24 (75%) Frame = -1 Query: 417 YLSSPDLYVVGESSAARVSGLKID 346 + S+PD+ V G+S AAR+ GL I+ Sbjct: 74 FFSNPDVGVKGDSIAARIEGLTIE 97 >At1g78010.1 68414.m09091 tRNA modification GTPase, putative similar to tRNA modification GTPase trmE [strain PCC 7120, Anabaena sp.] SWISS-PROT:Q8YN91 Length = 560 Score = 29.1 bits (62), Expect = 2.0 Identities = 16/64 (25%), Positives = 40/64 (62%), Gaps = 1/64 (1%) Frame = +2 Query: 272 SRPGKWGKPRTRLSGSLVAASASTTSILRPETRAAEDSPTTYKSGDDKYVFTLQLLV-EA 448 +R G+ +P ++ ++ +S+ TS+L+P +++E++ +K GD++ V + +V +A Sbjct: 23 TRGGRISRPISKNLCFVLFSSSPKTSLLKPRAQSSENNSLVFK-GDERVVGLVGKVVDDA 81 Query: 449 FSRL 460 F ++ Sbjct: 82 FDKV 85 >At5g50950.2 68418.m06319 fumarate hydratase, putative / fumarase, putative similar to SP|P55250 Fumarate hydratase, mitochondrial precursor (EC 4.2.1.2) (Fumarase) {Rhizopus oryzae}; contains Pfam profile PF00206: Lyase Length = 499 Score = 28.3 bits (60), Expect = 3.5 Identities = 26/91 (28%), Positives = 38/91 (41%), Gaps = 3/91 (3%) Frame = +1 Query: 10 LKDQKPGSLLQVGKMKFTLLFVLVIVTAVAADEEAGVR---SNGRPDLVIGTITSQDSLI 180 L + +PGS + GK+ T L +V A V SNG +L + +L+ Sbjct: 346 LPENEPGSSIMPGKVNPTQCEALTMVCAQVMGNHVAVTIGGSNGHFELNVFKPVIASALL 405 Query: 181 RSFRFNRPASPSSQSHFHRIAMAAGVRISAI 273 S R AS S + + R A RIS + Sbjct: 406 HSIRLIADASASFEKNCVRGIEANRERISKL 436 >At3g54220.1 68416.m05993 scarecrow transcription factor, putative nearly identical to SCARECROW [Arabidopsis thaliana] GI:1497987 Length = 653 Score = 28.3 bits (60), Expect = 3.5 Identities = 14/27 (51%), Positives = 15/27 (55%) Frame = +2 Query: 236 GSLWPPAFAYLPSRPGKWGKPRTRLSG 316 G WP F L SRPG G P RL+G Sbjct: 411 GLQWPGLFHILASRPG--GPPHVRLTG 435 >At4g33350.1 68417.m04741 chloroplast inner membrane import protein Tic22, putative similar to Tic22 [Pisum sativum] gi|3769671|gb|AAC64606 Length = 268 Score = 27.5 bits (58), Expect = 6.1 Identities = 22/53 (41%), Positives = 28/53 (52%), Gaps = 2/53 (3%) Frame = +2 Query: 200 DQRRLVRSL-TSTGSL-WPPAFAYLPSRPGKWGKPRTRLSGSLVAASASTTSI 352 D +R +L T SL PP FA + K G P T LS SLVA + + TS+ Sbjct: 34 DTKRFAETLATRRFSLPTPPPFASVSQ--SKSGTPTTTLSPSLVAKALAGTSV 84 >At3g01870.1 68416.m00132 hypothetical protein Length = 583 Score = 27.5 bits (58), Expect = 6.1 Identities = 17/52 (32%), Positives = 23/52 (44%) Frame = +2 Query: 233 TGSLWPPAFAYLPSRPGKWGKPRTRLSGSLVAASASTTSILRPETRAAEDSP 388 T +P A +PS G +GK R L G V + +TS + R E P Sbjct: 56 TAFTFPSALPVIPSGGGNFGKGRIDLGGLEVIQVSISTSTSQRVWRTYEGGP 107 >At3g13570.1 68416.m01707 SC35-like splicing factor, 30a kD (SCL30a) almost identical to SC35-like splicing factor SCL30a GI:9843661 from [Arabidopsis thaliana]; contains Pfam profile PF00076: RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain) Length = 262 Score = 27.1 bits (57), Expect = 8.1 Identities = 17/49 (34%), Positives = 24/49 (48%), Gaps = 1/49 (2%) Frame = +3 Query: 231 PQDR-YGRRRSHICHHGPGSGANPEPDYPGRWWRHRLQQRQS*GPKRAR 374 PQDR Y + RS+ P +G+ PGR H R+S P++ R Sbjct: 179 PQDRRYEKERSY-SRSPPHNGSRVRSGSPGRVKSHSRSPRRSVSPRKNR 226 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 12,151,745 Number of Sequences: 28952 Number of extensions: 264919 Number of successful extensions: 735 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 715 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 735 length of database: 12,070,560 effective HSP length: 77 effective length of database: 9,841,256 effective search space used: 1013649368 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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