BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fmgV11o17f (590 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC354.12 |gpd3||glyceraldehyde 3-phosphate dehydrogenase Gpd3|... 256 2e-69 SPBC32F12.11 |tdh1|gpd1|glyceraldehyde-3-phosphate dehydrogenase... 255 3e-69 SPAC31A2.05c |mis4||cohesin loading factor Mis4|Schizosaccharomy... 27 2.7 SPBC1347.05c |||DNAJ domain protein Scj1|Schizosaccharomyces pom... 26 4.7 SPAC6B12.02c |mus7||DNA repair protein Mus7|Schizosaccharomyces ... 25 6.2 SPBC577.07 |ubp10||ubiquitin C-terminal hydrolase Ubp10|Schizosa... 25 6.2 SPBC337.08c |ubi4||ubiquitin|Schizosaccharomyces pombe|chr 2|||M... 25 6.2 SPBC660.13c |ssb1|rpa1, rad11|DNA replication factor A subunit S... 25 6.2 >SPBC354.12 |gpd3||glyceraldehyde 3-phosphate dehydrogenase Gpd3|Schizosaccharomyces pombe|chr 2|||Manual Length = 335 Score = 256 bits (627), Expect = 2e-69 Identities = 123/168 (73%), Positives = 142/168 (84%), Gaps = 1/168 (0%) Frame = +2 Query: 89 KIGINGFGRIGRLVLRASIEKGA-QVVAINDPFIGLDYMVYLFKYDSTHGRFKGSVEVQD 265 K+GINGFGRIGR+VLR +I G QVVA+NDPFI LDYM Y+FKYDSTHGRF+GSVE + Sbjct: 5 KVGINGFGRIGRIVLRNAILTGKIQVVAVNDPFIDLDYMAYMFKYDSTHGRFEGSVETKG 64 Query: 266 GFLVVNGNKIAVFSERDPKAIPWGKAGAEYVVESTGVFTTTDKASAHLEGGAKKVIISAP 445 G LV++G+ I V +ERDP I W +GAEYV+ESTGVFTT + ASAHL+GGAK+VIISAP Sbjct: 65 GKLVIDGHSIDVHNERDPANIKWSASGAEYVIESTGVFTTKETASAHLKGGAKRVIISAP 124 Query: 446 SADAPMFVVGVNLEAYDPSFKVISNASCTTNCLAPLAKVIHDNFEIVE 589 S DAPMFVVGVNLE ++PS KVISNASCTTNCLAPLAKVI+D F I E Sbjct: 125 SKDAPMFVVGVNLEKFNPSEKVISNASCTTNCLAPLAKVINDTFGIEE 172 >SPBC32F12.11 |tdh1|gpd1|glyceraldehyde-3-phosphate dehydrogenase Tdh1|Schizosaccharomyces pombe|chr 2|||Manual Length = 336 Score = 255 bits (625), Expect = 3e-69 Identities = 122/168 (72%), Positives = 142/168 (84%), Gaps = 1/168 (0%) Frame = +2 Query: 89 KIGINGFGRIGRLVLR-ASIEKGAQVVAINDPFIGLDYMVYLFKYDSTHGRFKGSVEVQD 265 K+GINGFGRIGR+VLR A + K QVVAINDPFI L+YM Y+FKYDSTHGRF GSVE++D Sbjct: 5 KVGINGFGRIGRIVLRNALVAKTIQVVAINDPFIDLEYMAYMFKYDSTHGRFDGSVEIKD 64 Query: 266 GFLVVNGNKIAVFSERDPKAIPWGKAGAEYVVESTGVFTTTDKASAHLEGGAKKVIISAP 445 G LV++GN I V +ERDP I W +GA+YV+ESTGVFTT + ASAHL+GGAK+VIISAP Sbjct: 65 GKLVIDGNAIDVHNERDPADIKWSTSGADYVIESTGVFTTQETASAHLKGGAKRVIISAP 124 Query: 446 SADAPMFVVGVNLEAYDPSFKVISNASCTTNCLAPLAKVIHDNFEIVE 589 S DAPM+VVGVN E ++PS KVISNASCTTNCLAPLAKVI+D F I E Sbjct: 125 SKDAPMYVVGVNEEKFNPSEKVISNASCTTNCLAPLAKVINDTFGIEE 172 >SPAC31A2.05c |mis4||cohesin loading factor Mis4|Schizosaccharomyces pombe|chr 1|||Manual Length = 1583 Score = 26.6 bits (56), Expect = 2.7 Identities = 18/55 (32%), Positives = 29/55 (52%), Gaps = 3/55 (5%) Frame = -2 Query: 295 NFVTVNNKESILNLNTALKTAMGGIILEKINHIVK---TDERVIYSDHLSSLFNR 140 +F + N+ESIL+L L + + E I++IV+ +D R HLS L + Sbjct: 338 SFKKLQNEESILHLLNILHSIFEYTVPEAIDNIVQSKTSDARTSEIQHLSVLLQK 392 >SPBC1347.05c |||DNAJ domain protein Scj1|Schizosaccharomyces pombe|chr 2|||Manual Length = 381 Score = 25.8 bits (54), Expect = 4.7 Identities = 15/62 (24%), Positives = 29/62 (46%), Gaps = 1/62 (1%) Frame = -2 Query: 454 ISTGS*YNNFFSTSLQVSRCFICSGKDTSRLYNIFSPSFSPWNGLRVPF*E-NGNFVTVN 278 + +G +N +S + C +C G + ++ +P F +R+P NGN T+ Sbjct: 153 VCSGQGFNPKYSADKAIESCPVCGGSGFRVIEHMIAPGFR--QQMRMPCNACNGNGRTIK 210 Query: 277 NK 272 +K Sbjct: 211 HK 212 >SPAC6B12.02c |mus7||DNA repair protein Mus7|Schizosaccharomyces pombe|chr 1|||Manual Length = 1888 Score = 25.4 bits (53), Expect = 6.2 Identities = 18/65 (27%), Positives = 33/65 (50%), Gaps = 1/65 (1%) Frame = -2 Query: 298 GNFVTVNNKESILNLNTALKTAMGGIILEKINHIVKTDERVIYSDHL-SSLFNRSTEHQT 122 GN +VN+K+ +N+N KTA ++H+ E ++ L SSLF+ + ++ Sbjct: 519 GNIFSVNSKKHSVNINA--KTAAN----NGLSHLQNFSEELLKKRKLFSSLFSNNVSYKK 572 Query: 121 ANAAK 107 + K Sbjct: 573 SKKLK 577 >SPBC577.07 |ubp10||ubiquitin C-terminal hydrolase Ubp10|Schizosaccharomyces pombe|chr 2|||Manual Length = 502 Score = 25.4 bits (53), Expect = 6.2 Identities = 14/37 (37%), Positives = 19/37 (51%) Frame = +2 Query: 104 GFGRIGRLVLRASIEKGAQVVAINDPFIGLDYMVYLF 214 GF RI +R EKG QVV D I + + +L+ Sbjct: 298 GFVRIESQKIRQHAEKGEQVVFTGDRVIQTNVVPFLY 334 >SPBC337.08c |ubi4||ubiquitin|Schizosaccharomyces pombe|chr 2|||Manual Length = 382 Score = 25.4 bits (53), Expect = 6.2 Identities = 11/16 (68%), Positives = 13/16 (81%) Frame = +1 Query: 13 LHLVLSVRGGGQLFNK 60 LHLVL +RGG Q+F K Sbjct: 67 LHLVLRLRGGMQIFVK 82 Score = 25.4 bits (53), Expect = 6.2 Identities = 11/16 (68%), Positives = 13/16 (81%) Frame = +1 Query: 13 LHLVLSVRGGGQLFNK 60 LHLVL +RGG Q+F K Sbjct: 143 LHLVLRLRGGMQIFVK 158 Score = 25.4 bits (53), Expect = 6.2 Identities = 11/16 (68%), Positives = 13/16 (81%) Frame = +1 Query: 13 LHLVLSVRGGGQLFNK 60 LHLVL +RGG Q+F K Sbjct: 219 LHLVLRLRGGMQIFVK 234 Score = 25.4 bits (53), Expect = 6.2 Identities = 11/16 (68%), Positives = 13/16 (81%) Frame = +1 Query: 13 LHLVLSVRGGGQLFNK 60 LHLVL +RGG Q+F K Sbjct: 295 LHLVLRLRGGMQIFVK 310 >SPBC660.13c |ssb1|rpa1, rad11|DNA replication factor A subunit Ssb1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 609 Score = 25.4 bits (53), Expect = 6.2 Identities = 16/77 (20%), Positives = 38/77 (49%), Gaps = 6/77 (7%) Frame = +2 Query: 71 LADNMSKIGINGFGRIGRLVLRASIEKGAQVVAINDPFIGLD------YMVYLFKYDSTH 232 + D+ ++ +N F +G+L++ + ++ + ND ++ YM Y+F+ + Sbjct: 515 VGDHTGQLWLNVFDDVGKLIMHKTADE-LNDLQENDENAFMNCMAEACYMPYIFQCRAKQ 573 Query: 233 GRFKGSVEVQDGFLVVN 283 FKG + V+ + +N Sbjct: 574 DNFKGEMRVRYTVMSIN 590 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,573,541 Number of Sequences: 5004 Number of extensions: 54758 Number of successful extensions: 188 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 179 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 186 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 256184654 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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