Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fmgV11o15r
(732 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g18110.1 68415.m02105 elongation factor 1-beta, putative / EF... 156 2e-38
At1g30230.1 68414.m03698 elongation factor 1-beta / EF-1-beta id... 153 2e-37
At5g12110.1 68418.m01422 elongation factor 1B alpha-subunit 1 (e... 145 3e-35
At5g19510.1 68418.m02324 elongation factor 1B alpha-subunit 2 (e... 144 5e-35
At2g40660.1 68415.m05017 tRNA-binding region domain-containing p... 35 0.064
At1g70330.1 68414.m08091 equilibrative nucleoside transporter fa... 31 0.79
At2g34400.1 68415.m04215 pentatricopeptide (PPR) repeat-containi... 31 1.0
At5g41315.1 68418.m05021 basic helix-loop-helix (bHLH) family pr... 30 1.4
At5g64960.1 68418.m08171 cyclin-dependent kinase, putative / CDK... 30 1.8
At5g38990.1 68418.m04717 protein kinase family protein contains ... 29 4.2
At5g08415.1 68418.m00991 lipoic acid synthase family protein sim... 29 4.2
At3g46390.1 68416.m05029 hypothetical protein 28 5.6
At2g05170.1 68415.m00544 vacuolar protein sorting 11 family prot... 28 5.6
At1g33010.1 68414.m04065 F-box family protein 28 5.6
At1g68210.1 68414.m07792 two-component responsive regulator fami... 28 7.3
At5g39000.1 68418.m04718 protein kinase family protein contains ... 27 9.7
At2g36190.1 68415.m04442 beta-fructosidase, putative / beta-fruc... 27 9.7
>At2g18110.1 68415.m02105 elongation factor 1-beta, putative /
EF-1-beta, putative nearly identical to eEF-1beta
[Arabidopsis thaliana] GI:398606
Length = 231
Score = 156 bits (378), Expect = 2e-38
Identities = 93/235 (39%), Positives = 128/235 (54%), Gaps = 14/235 (5%)
Frame = -2
Query: 677 AVGDVKTAQGLNDLNQYLAEKSYVSGYTPSQADVQVFEQVGKAPAANLPHVLRWYNQI-- 504
A ++ + GL L+++L +SY++GY S+ D+ VF + K P + +V RW+N I
Sbjct: 3 AFPNLNSGSGLKKLDEHLLTRSYITGYQASKDDITVFTALSKPPTSEFVNVSRWFNHIDA 62
Query: 503 ---ASYTPAERK-TWSQGTSPLXXXXXXXXXXXXXXXXXXXD-----VDLFGSGXXXXXX 351
S AE +G+SP+ + VDLFG
Sbjct: 63 LLRISGVSAEGSGVIVEGSSPITEEAVATPPAADSKDTAAEEEDDDDVDLFGEETEEEKK 122
Query: 350 XXXXXXXXRLKAYADKKSKKPALIAKSSILLDVKPWDDETDMKEMENQVRTIEMEGLLWG 171
+A + K S K KSS+L+D+KPWDDETDMK++E VR+I+MEGL WG
Sbjct: 123 AAEE------RAASVKASTKKKESGKSSVLMDIKPWDDETDMKKLEEAVRSIQMEGLFWG 176
Query: 170 ASKLVPVGYGINKLQIMCVIEDDKVSVDLLTEK---IQEFEDFVQSVDIAAFNKI 15
ASKLVPVGYGI KL IMC I DD VS+D + E+ ++ ++VQS DI AFNKI
Sbjct: 177 ASKLVPVGYGIKKLHIMCTIVDDLVSIDTMIEEQLTVEPINEYVQSCDIVAFNKI 231
>At1g30230.1 68414.m03698 elongation factor 1-beta / EF-1-beta
identical to SP|P48006 Elongation factor 1-beta
(EF-1-beta) {Arabidopsis thaliana}
Length = 231
Score = 153 bits (370), Expect = 2e-37
Identities = 91/235 (38%), Positives = 128/235 (54%), Gaps = 14/235 (5%)
Frame = -2
Query: 677 AVGDVKTAQGLNDLNQYLAEKSYVSGYTPSQADVQVFEQVGKAPAANLPHVLRWYNQI-- 504
A ++ + GL L+++L +SY++GY S+ D+ VF + K P + + RWYN I
Sbjct: 3 AFPNLNSDAGLKKLDEHLLTRSYITGYQASKDDITVFAALAKPPTSQYVNASRWYNHIDA 62
Query: 503 ---ASYTPAERK-TWSQGTSPLXXXXXXXXXXXXXXXXXXXD-----VDLFGSGXXXXXX 351
S AE +G++P+ + VDLFG
Sbjct: 63 LLRISGVSAEGSGVIVEGSAPITEEAVATPPAADSKDAAADEEDDDDVDLFGEETEEEKK 122
Query: 350 XXXXXXXXRLKAYADKKSKKPALIAKSSILLDVKPWDDETDMKEMENQVRTIEMEGLLWG 171
+A + K S K KSS+L+D+KPWDDETDMK++E V++I+MEGL WG
Sbjct: 123 AAEE------RAASVKASTKKKESGKSSVLIDIKPWDDETDMKKLEEAVKSIQMEGLFWG 176
Query: 170 ASKLVPVGYGINKLQIMCVIEDDKVSVDLLTEK---IQEFEDFVQSVDIAAFNKI 15
ASKLVPVGYGI KLQI+C I DD VS+D + E+ ++ ++VQS DI AFNKI
Sbjct: 177 ASKLVPVGYGIKKLQILCTIVDDLVSIDTMIEEQLTVEPINEYVQSCDIVAFNKI 231
>At5g12110.1 68418.m01422 elongation factor 1B alpha-subunit 1
(eEF1Balpha1) identical to elongation factor 1B
alpha-subunit [Arabidopsis thaliana] GI:6686819
Length = 228
Score = 145 bits (351), Expect = 3e-35
Identities = 91/228 (39%), Positives = 119/228 (52%), Gaps = 10/228 (4%)
Frame = -2
Query: 668 DVKTAQGLNDLNQYLAEKSYVSGYTPSQADVQVFEQVGKAPAANLPHVLRWYNQIASYTP 489
D+ T +GL L ++LA K+Y+SG S DV+V+ V + P P+ +WY+ +AS+
Sbjct: 7 DLHTERGLKTLEEHLAGKTYISGDQLSVDDVKVYAAVLENPGDGFPNASKWYDSVASHLA 66
Query: 488 AERKTWSQGTS-------PLXXXXXXXXXXXXXXXXXXXDVDLFGSGXXXXXXXXXXXXX 330
+ G P D+DLF
Sbjct: 67 KSFPGKADGVRVGGGVAPPSEAHPHTEEPAADGDGDDDDDIDLFADETEDEKKAAEE--- 123
Query: 329 XRLKAYADKKSKKPALIAKSSILLDVKPWDDETDMKEMENQVRTIEMEGLLWGASKLVPV 150
+ A K +KK KSS+LL+VKPWDDETDMK++E VR+++M GL WGASKLVPV
Sbjct: 124 ---REAAKKDTKKTKESGKSSVLLEVKPWDDETDMKKLEEAVRSVQMPGLTWGASKLVPV 180
Query: 149 GYGINKLQIMCVIEDDKVSVDLLTEKIQEFE---DFVQSVDIAAFNKI 15
GYGI KL IM I DD VSVD L E E +++QSVDI AFNKI
Sbjct: 181 GYGIKKLTIMMTIVDDLVSVDNLIEDHLTSEPNNEYIQSVDIVAFNKI 228
>At5g19510.1 68418.m02324 elongation factor 1B alpha-subunit 2
(eEF1Balpha2) identical to elongation factor 1B
alpha-subunit [Arabidopsis thaliana] GI:6686821
Length = 224
Score = 144 bits (349), Expect = 5e-35
Identities = 89/224 (39%), Positives = 115/224 (51%), Gaps = 6/224 (2%)
Frame = -2
Query: 668 DVKTAQGLNDLNQYLAEKSYVSGYTPSQADVQVFEQVGKAPAANLPHVLRWYNQIASYTP 489
D+ T +G+ + ++LA K+Y+SG S DV+V+ V P+ P+ +WY +AS
Sbjct: 7 DLHTEEGVKSVEEHLAGKTYISGDQLSVDDVKVYAAVPVKPSDAFPNASKWYESVASQLA 66
Query: 488 AE---RKTWSQGTSPLXXXXXXXXXXXXXXXXXXXDVDLFGSGXXXXXXXXXXXXXXRLK 318
+ Q D+DLFG +
Sbjct: 67 KSFPGKAVGVQFGGSAAAAPAVEAEAPAAAADDDDDMDLFGDETEEEKKAAEE------R 120
Query: 317 AYADKKSKKPALIAKSSILLDVKPWDDETDMKEMENQVRTIEMEGLLWGASKLVPVGYGI 138
A K +KKP KSS+L+DVKPWDDETDMK++E VR +EM GL WGASKLVPVGYGI
Sbjct: 121 EAAKKDTKKPKESGKSSVLMDVKPWDDETDMKKLEEAVRGVEMPGLFWGASKLVPVGYGI 180
Query: 137 NKLQIMCVIEDDKVSVDLLTEKIQEFE---DFVQSVDIAAFNKI 15
KL IM I DD VS D L E E +++QS DI AFNKI
Sbjct: 181 KKLTIMFTIVDDLVSPDNLIEDFLTSEPNNEYIQSCDIVAFNKI 224
>At2g40660.1 68415.m05017 tRNA-binding region domain-containing
protein similar to SP|Q12904 Multisynthetase complex
auxiliary component p43 [Contains: Endothelial-monocyte
activating polypeptide II (EMAP-II) (Small inducible
cytokine subfamily E member 1)] {Homo sapiens}; contains
Pfam profile PF01588: Putative tRNA binding domain
Length = 389
Score = 34.7 bits (76), Expect = 0.064
Identities = 23/57 (40%), Positives = 29/57 (50%), Gaps = 9/57 (15%)
Frame = -2
Query: 647 LNDLNQYLAEKSYV--SGYTPSQADVQVFEQV-------GKAPAANLPHVLRWYNQI 504
L LN LA KS + +G TPS ADV VF + + +PHV+RW N I
Sbjct: 76 LEKLNLELATKSVLLGNGLTPSAADVAVFSALHSSVLGLSDSDKEKVPHVIRWVNYI 132
>At1g70330.1 68414.m08091 equilibrative nucleoside transporter
family protein contains similarity to SWISS-PROT:Q14542
equilibrative nucleoside transporter 2 (Equilibrative
nitrobenzylmercaptopurine riboside-insensitive
nucleoside transporter, Equilibrative NBMPR-insensitive
nucleoside transporter, Nucleoside transporter, ei-type,
36 kDa nucleolar protein HNP36, Hydrophobic nucleolar
protein, 36 kDa, Delayed-early response protein 12)
[Homo sapiens]
Length = 450
Score = 31.1 bits (67), Expect = 0.79
Identities = 10/26 (38%), Positives = 19/26 (73%)
Frame = +3
Query: 171 SPEKAFHFNSAYLVFHFLHIGFIIPW 248
+P ++HF AY+++ L +GF++PW
Sbjct: 56 APSDSYHF--AYIIYFTLGVGFLLPW 79
>At2g34400.1 68415.m04215 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 617
Score = 30.7 bits (66), Expect = 1.0
Identities = 16/72 (22%), Positives = 32/72 (44%)
Frame = -2
Query: 281 IAKSSILLDVKPWDDETDMKEMENQVRTIEMEGLLWGASKLVPVGYGINKLQIMCVIEDD 102
+ S++L D+K WD+ M+ + ++ G W + + + + C ED
Sbjct: 535 VISSNVLADMKMWDESAKMRALMRDRGVVKTPGCSWIEIEGELMEFLAGSDYLQCGREDS 594
Query: 101 KVSVDLLTEKIQ 66
DLL E+++
Sbjct: 595 GSLFDLLVEEMK 606
>At5g41315.1 68418.m05021 basic helix-loop-helix (bHLH) family
protein contains Pfam profile: PF00010 helix-loop-helix
DNA-binding domain ;annotation temporarily based on
supporting cDNA gi|17224394|gb|AF246291.1|AF246291
Length = 637
Score = 30.3 bits (65), Expect = 1.4
Identities = 20/85 (23%), Positives = 38/85 (44%)
Frame = -2
Query: 290 PALIAKSSILLDVKPWDDETDMKEMENQVRTIEMEGLLWGASKLVPVGYGINKLQIMCVI 111
P + K ++LD DET + + R ++ K++P INK+ + ++
Sbjct: 420 PRVHQKEKLMLDSPEARDETGNHAVLEKKRREKLNERFMTLRKIIP---SINKIDKVSIL 476
Query: 110 EDDKVSVDLLTEKIQEFEDFVQSVD 36
+D + L ++QE E +S D
Sbjct: 477 DDTIEYLQELERRVQELESCRESTD 501
>At5g64960.1 68418.m08171 cyclin-dependent kinase, putative / CDK,
putative similar to cyclin dependent kinase C
[Lycopersicon esculentum] gi|15215944|emb|CAC51391
Length = 513
Score = 29.9 bits (64), Expect = 1.8
Identities = 16/54 (29%), Positives = 28/54 (51%), Gaps = 2/54 (3%)
Frame = -2
Query: 632 QYLAEKSYVSGYTPSQADVQVFEQVGKAPAANLPHVLR--WYNQIASYTPAERK 477
+ L K + G T ++ +++E G +N P V + WYNQ+ S P +R+
Sbjct: 233 ELLNGKPILPGKTENEQLNKIYELCGSPDESNWPGVSKMPWYNQMKSSRPLKRR 286
>At5g38990.1 68418.m04717 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 880
Score = 28.7 bits (61), Expect = 4.2
Identities = 16/40 (40%), Positives = 23/40 (57%)
Frame = -3
Query: 535 FPTYYDGIIKLLHTLRLNAKLGLRAPAH*PPVLNPRLPPQ 416
+PTYYD I+ + L+L+ G A + P L+P PPQ
Sbjct: 387 YPTYYDAILSGVEILKLSNSDGNLAGLNPIPQLSP--PPQ 424
>At5g08415.1 68418.m00991 lipoic acid synthase family protein
similar to lipoic acid synthase from Arabidopsis
thaliana [gi:3928758], from Mus musculus [gi:14669826]
Pfam profile PF04055: radical SAM domain protein
Length = 394
Score = 28.7 bits (61), Expect = 4.2
Identities = 16/44 (36%), Positives = 26/44 (59%)
Frame = -2
Query: 302 KSKKPALIAKSSILLDVKPWDDETDMKEMENQVRTIEMEGLLWG 171
K KP +I K+SI+L + D+E +KE +R I+++ L G
Sbjct: 287 KISKPGMITKTSIMLGLGETDEE--LKEAMADLRAIDVDILTLG 328
>At3g46390.1 68416.m05029 hypothetical protein
Length = 69
Score = 28.3 bits (60), Expect = 5.6
Identities = 19/69 (27%), Positives = 33/69 (47%), Gaps = 2/69 (2%)
Frame = -2
Query: 323 LKAYADKKSKKPALIAKSSILLDVKPWDDETDMKEMENQVRTIEMEGLLWGA--SKLVPV 150
+K Y+ K K+ + +I +D+K +M + E + I M G++W S L+ V
Sbjct: 1 MKRYSTKHFKRILIANNVNIDIDLKTGIVAKNMAKKEKIEQVISMGGIMWEGLNSSLIKV 60
Query: 149 GYGINKLQI 123
+ K QI
Sbjct: 61 DEALLKQQI 69
>At2g05170.1 68415.m00544 vacuolar protein sorting 11 family protein
/ VPS11 family protein similar to Vacuolar protein
sorting 11 (hVPS11) (PP3476) (Swiss-Prot:Q9H270) [Homo
sapiens]; similar to Vacuolar biogenesis protein END1
(PEP5 protein) (Vacuolar protein sorting 11)
(Swiss-Prot:P12868) [Saccharomyces cerevisiae]
Length = 932
Score = 28.3 bits (60), Expect = 5.6
Identities = 16/32 (50%), Positives = 17/32 (53%)
Frame = -2
Query: 647 LNDLNQYLAEKSYVSGYTPSQADVQVFEQVGK 552
L DL Y YVS PSQA V + EQ GK
Sbjct: 495 LEDLGNYDEALQYVSSLEPSQAGVTI-EQYGK 525
>At1g33010.1 68414.m04065 F-box family protein
Length = 361
Score = 28.3 bits (60), Expect = 5.6
Identities = 16/50 (32%), Positives = 25/50 (50%)
Frame = -2
Query: 251 KPWDDETDMKEMENQVRTIEMEGLLWGASKLVPVGYGINKLQIMCVIEDD 102
KP ET M ++QVRTI+ + + NK +MC+++DD
Sbjct: 317 KPTSTETSMSRKDHQVRTIDQPQQDRCTFESIN-----NKFDVMCLLDDD 361
>At1g68210.1 68414.m07792 two-component responsive regulator family
protein / response regulator family protein contains
Pfam profile: PF00072 response regulator receiver domain
Length = 663
Score = 27.9 bits (59), Expect = 7.3
Identities = 12/30 (40%), Positives = 19/30 (63%)
Frame = -2
Query: 116 VIEDDKVSVDLLTEKIQEFEDFVQSVDIAA 27
+I+ D S+ LT +Q+F V SVD+A+
Sbjct: 17 LIDHDTASIASLTSMLQQFSKRVMSVDVAS 46
>At5g39000.1 68418.m04718 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 873
Score = 27.5 bits (58), Expect = 9.7
Identities = 14/56 (25%), Positives = 28/56 (50%)
Frame = -3
Query: 532 PTYYDGIIKLLHTLRLNAKLGLRAPAH*PPVLNPRLPPQQRKTTMTTTLIYLVLVT 365
P YYD I+ + L++N G A + P+++P L P + + +++ +T
Sbjct: 390 PKYYDAILNGVEILKMNDPDGNLAGPNPDPLVSPDLIPNRATPRIRKNKSHILPIT 445
>At2g36190.1 68415.m04442 beta-fructosidase, putative /
beta-fructofuranosidase, putative similar to
beta-fructofuranosidase GI:18324 from [Daucus carota]
Length = 591
Score = 27.5 bits (58), Expect = 9.7
Identities = 13/38 (34%), Positives = 22/38 (57%)
Frame = -2
Query: 647 LNDLNQYLAEKSYVSGYTPSQADVQVFEQVGKAPAANL 534
+N+ + + ++ V G TP+QADV+V VG A +
Sbjct: 386 MNNHDIKMGQRIEVKGITPAQADVEVTFYVGSLEKAEI 423
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,591,539
Number of Sequences: 28952
Number of extensions: 296017
Number of successful extensions: 907
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 875
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 902
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1604469728
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -