Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fmgV11o15f
(563 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g18110.1 68415.m02105 elongation factor 1-beta, putative / EF... 93 2e-19
At5g19510.1 68418.m02324 elongation factor 1B alpha-subunit 2 (e... 91 6e-19
At1g30230.1 68414.m03698 elongation factor 1-beta / EF-1-beta id... 89 3e-18
At5g12110.1 68418.m01422 elongation factor 1B alpha-subunit 1 (e... 88 4e-18
At2g40660.1 68415.m05017 tRNA-binding region domain-containing p... 35 0.043
At1g70330.1 68414.m08091 equilibrative nucleoside transporter fa... 31 0.53
At5g64960.1 68418.m08171 cyclin-dependent kinase, putative / CDK... 30 1.2
At5g38990.1 68418.m04717 protein kinase family protein contains ... 29 2.8
At5g08415.1 68418.m00991 lipoic acid synthase family protein sim... 29 2.8
At2g05170.1 68415.m00544 vacuolar protein sorting 11 family prot... 28 3.7
At5g39000.1 68418.m04718 protein kinase family protein contains ... 27 6.5
At2g36190.1 68415.m04442 beta-fructosidase, putative / beta-fruc... 27 6.5
At2g34400.1 68415.m04215 pentatricopeptide (PPR) repeat-containi... 27 6.5
At2g36270.1 68415.m04452 bZIP transcription factor family protei... 27 8.7
>At2g18110.1 68415.m02105 elongation factor 1-beta, putative /
EF-1-beta, putative nearly identical to eEF-1beta
[Arabidopsis thaliana] GI:398606
Length = 231
Score = 92.7 bits (220), Expect = 2e-19
Identities = 60/180 (33%), Positives = 87/180 (48%), Gaps = 11/180 (6%)
Frame = +1
Query: 55 AVGDVKTAQGLNDLNQYLAEKSYVSGYTPSQADVQVFEQVGKAPAANLPHVLRWYNQI-- 228
A ++ + GL L+++L +SY++GY S+ D+ VF + K P + +V RW+N I
Sbjct: 3 AFPNLNSGSGLKKLDEHLLTRSYITGYQASKDDITVFTALSKPPTSEFVNVSRWFNHIDA 62
Query: 229 ---ASYTPAERK-TWSQGTSPLXXXXXXXXXXXXXXXXXXXX-----VDLFGSGXXXXXX 381
S AE +G+SP+ VDLFG
Sbjct: 63 LLRISGVSAEGSGVIVEGSSPITEEAVATPPAADSKDTAAEEEDDDDVDLFGEETEEEKK 122
Query: 382 XXXXXXXXXLKAYADKKSKKPALIAKSSILLDVKPWDDETDMKEMENQVRTIEMEGLLWG 561
+A + K S K KSS+L+D+KPWDDETDMK++E VR+I+MEGL WG
Sbjct: 123 AAEE------RAASVKASTKKKESGKSSVLMDIKPWDDETDMKKLEEAVRSIQMEGLFWG 176
>At5g19510.1 68418.m02324 elongation factor 1B alpha-subunit 2
(eEF1Balpha2) identical to elongation factor 1B
alpha-subunit [Arabidopsis thaliana] GI:6686821
Length = 224
Score = 90.6 bits (215), Expect = 6e-19
Identities = 55/169 (32%), Positives = 78/169 (46%), Gaps = 3/169 (1%)
Frame = +1
Query: 64 DVKTAQGLNDLNQYLAEKSYVSGYTPSQADVQVFEQVGKAPAANLPHVLRWYNQIASYTP 243
D+ T +G+ + ++LA K+Y+SG S DV+V+ V P+ P+ +WY +AS
Sbjct: 7 DLHTEEGVKSVEEHLAGKTYISGDQLSVDDVKVYAAVPVKPSDAFPNASKWYESVASQLA 66
Query: 244 AE---RKTWSQGTSPLXXXXXXXXXXXXXXXXXXXXVDLFGSGXXXXXXXXXXXXXXXLK 414
+ Q +DLFG +
Sbjct: 67 KSFPGKAVGVQFGGSAAAAPAVEAEAPAAAADDDDDMDLFGDETEEEKKAAEE------R 120
Query: 415 AYADKKSKKPALIAKSSILLDVKPWDDETDMKEMENQVRTIEMEGLLWG 561
A K +KKP KSS+L+DVKPWDDETDMK++E VR +EM GL WG
Sbjct: 121 EAAKKDTKKPKESGKSSVLMDVKPWDDETDMKKLEEAVRGVEMPGLFWG 169
>At1g30230.1 68414.m03698 elongation factor 1-beta / EF-1-beta
identical to SP|P48006 Elongation factor 1-beta
(EF-1-beta) {Arabidopsis thaliana}
Length = 231
Score = 88.6 bits (210), Expect = 3e-18
Identities = 58/180 (32%), Positives = 86/180 (47%), Gaps = 11/180 (6%)
Frame = +1
Query: 55 AVGDVKTAQGLNDLNQYLAEKSYVSGYTPSQADVQVFEQVGKAPAANLPHVLRWYNQI-- 228
A ++ + GL L+++L +SY++GY S+ D+ VF + K P + + RWYN I
Sbjct: 3 AFPNLNSDAGLKKLDEHLLTRSYITGYQASKDDITVFAALAKPPTSQYVNASRWYNHIDA 62
Query: 229 ---ASYTPAERK-TWSQGTSPLXXXXXXXXXXXXXXXXXXXX-----VDLFGSGXXXXXX 381
S AE +G++P+ VDLFG
Sbjct: 63 LLRISGVSAEGSGVIVEGSAPITEEAVATPPAADSKDAAADEEDDDDVDLFGEETEEEKK 122
Query: 382 XXXXXXXXXLKAYADKKSKKPALIAKSSILLDVKPWDDETDMKEMENQVRTIEMEGLLWG 561
+A + K S K KSS+L+D+KPWDDETDMK++E V++I+MEGL WG
Sbjct: 123 AAEE------RAASVKASTKKKESGKSSVLIDIKPWDDETDMKKLEEAVKSIQMEGLFWG 176
>At5g12110.1 68418.m01422 elongation factor 1B alpha-subunit 1
(eEF1Balpha1) identical to elongation factor 1B
alpha-subunit [Arabidopsis thaliana] GI:6686819
Length = 228
Score = 87.8 bits (208), Expect = 4e-18
Identities = 55/173 (31%), Positives = 80/173 (46%), Gaps = 7/173 (4%)
Frame = +1
Query: 64 DVKTAQGLNDLNQYLAEKSYVSGYTPSQADVQVFEQVGKAPAANLPHVLRWYNQIASYTP 243
D+ T +GL L ++LA K+Y+SG S DV+V+ V + P P+ +WY+ +AS+
Sbjct: 7 DLHTERGLKTLEEHLAGKTYISGDQLSVDDVKVYAAVLENPGDGFPNASKWYDSVASHLA 66
Query: 244 AERKTWSQGTS-------PLXXXXXXXXXXXXXXXXXXXXVDLFGSGXXXXXXXXXXXXX 402
+ G P +DLF
Sbjct: 67 KSFPGKADGVRVGGGVAPPSEAHPHTEEPAADGDGDDDDDIDLFADETEDEKKAAEE--- 123
Query: 403 XXLKAYADKKSKKPALIAKSSILLDVKPWDDETDMKEMENQVRTIEMEGLLWG 561
+ A K +KK KSS+LL+VKPWDDETDMK++E VR+++M GL WG
Sbjct: 124 ---REAAKKDTKKTKESGKSSVLLEVKPWDDETDMKKLEEAVRSVQMPGLTWG 173
>At2g40660.1 68415.m05017 tRNA-binding region domain-containing
protein similar to SP|Q12904 Multisynthetase complex
auxiliary component p43 [Contains: Endothelial-monocyte
activating polypeptide II (EMAP-II) (Small inducible
cytokine subfamily E member 1)] {Homo sapiens}; contains
Pfam profile PF01588: Putative tRNA binding domain
Length = 389
Score = 34.7 bits (76), Expect = 0.043
Identities = 23/57 (40%), Positives = 29/57 (50%), Gaps = 9/57 (15%)
Frame = +1
Query: 85 LNDLNQYLAEKSYV--SGYTPSQADVQVFEQV-------GKAPAANLPHVLRWYNQI 228
L LN LA KS + +G TPS ADV VF + + +PHV+RW N I
Sbjct: 76 LEKLNLELATKSVLLGNGLTPSAADVAVFSALHSSVLGLSDSDKEKVPHVIRWVNYI 132
>At1g70330.1 68414.m08091 equilibrative nucleoside transporter
family protein contains similarity to SWISS-PROT:Q14542
equilibrative nucleoside transporter 2 (Equilibrative
nitrobenzylmercaptopurine riboside-insensitive
nucleoside transporter, Equilibrative NBMPR-insensitive
nucleoside transporter, Nucleoside transporter, ei-type,
36 kDa nucleolar protein HNP36, Hydrophobic nucleolar
protein, 36 kDa, Delayed-early response protein 12)
[Homo sapiens]
Length = 450
Score = 31.1 bits (67), Expect = 0.53
Identities = 10/26 (38%), Positives = 19/26 (73%)
Frame = -3
Query: 561 SPEKAFHFNSAYLVFHFLHIGFIIPW 484
+P ++HF AY+++ L +GF++PW
Sbjct: 56 APSDSYHF--AYIIYFTLGVGFLLPW 79
>At5g64960.1 68418.m08171 cyclin-dependent kinase, putative / CDK,
putative similar to cyclin dependent kinase C
[Lycopersicon esculentum] gi|15215944|emb|CAC51391
Length = 513
Score = 29.9 bits (64), Expect = 1.2
Identities = 16/54 (29%), Positives = 28/54 (51%), Gaps = 2/54 (3%)
Frame = +1
Query: 100 QYLAEKSYVSGYTPSQADVQVFEQVGKAPAANLPHVLR--WYNQIASYTPAERK 255
+ L K + G T ++ +++E G +N P V + WYNQ+ S P +R+
Sbjct: 233 ELLNGKPILPGKTENEQLNKIYELCGSPDESNWPGVSKMPWYNQMKSSRPLKRR 286
>At5g38990.1 68418.m04717 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 880
Score = 28.7 bits (61), Expect = 2.8
Identities = 16/40 (40%), Positives = 23/40 (57%)
Frame = +2
Query: 197 FPTYYDGIIKLLHTLRLNAKLGLRAPAH*PPVLNPRLPPQ 316
+PTYYD I+ + L+L+ G A + P L+P PPQ
Sbjct: 387 YPTYYDAILSGVEILKLSNSDGNLAGLNPIPQLSP--PPQ 424
>At5g08415.1 68418.m00991 lipoic acid synthase family protein
similar to lipoic acid synthase from Arabidopsis
thaliana [gi:3928758], from Mus musculus [gi:14669826]
Pfam profile PF04055: radical SAM domain protein
Length = 394
Score = 28.7 bits (61), Expect = 2.8
Identities = 16/44 (36%), Positives = 26/44 (59%)
Frame = +1
Query: 430 KSKKPALIAKSSILLDVKPWDDETDMKEMENQVRTIEMEGLLWG 561
K KP +I K+SI+L + D+E +KE +R I+++ L G
Sbjct: 287 KISKPGMITKTSIMLGLGETDEE--LKEAMADLRAIDVDILTLG 328
>At2g05170.1 68415.m00544 vacuolar protein sorting 11 family protein
/ VPS11 family protein similar to Vacuolar protein
sorting 11 (hVPS11) (PP3476) (Swiss-Prot:Q9H270) [Homo
sapiens]; similar to Vacuolar biogenesis protein END1
(PEP5 protein) (Vacuolar protein sorting 11)
(Swiss-Prot:P12868) [Saccharomyces cerevisiae]
Length = 932
Score = 28.3 bits (60), Expect = 3.7
Identities = 16/32 (50%), Positives = 17/32 (53%)
Frame = +1
Query: 85 LNDLNQYLAEKSYVSGYTPSQADVQVFEQVGK 180
L DL Y YVS PSQA V + EQ GK
Sbjct: 495 LEDLGNYDEALQYVSSLEPSQAGVTI-EQYGK 525
>At5g39000.1 68418.m04718 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 873
Score = 27.5 bits (58), Expect = 6.5
Identities = 14/56 (25%), Positives = 28/56 (50%)
Frame = +2
Query: 200 PTYYDGIIKLLHTLRLNAKLGLRAPAH*PPVLNPRLPPQQRKTTMTTTLIYLVLVT 367
P YYD I+ + L++N G A + P+++P L P + + +++ +T
Sbjct: 390 PKYYDAILNGVEILKMNDPDGNLAGPNPDPLVSPDLIPNRATPRIRKNKSHILPIT 445
>At2g36190.1 68415.m04442 beta-fructosidase, putative /
beta-fructofuranosidase, putative similar to
beta-fructofuranosidase GI:18324 from [Daucus carota]
Length = 591
Score = 27.5 bits (58), Expect = 6.5
Identities = 13/38 (34%), Positives = 22/38 (57%)
Frame = +1
Query: 85 LNDLNQYLAEKSYVSGYTPSQADVQVFEQVGKAPAANL 198
+N+ + + ++ V G TP+QADV+V VG A +
Sbjct: 386 MNNHDIKMGQRIEVKGITPAQADVEVTFYVGSLEKAEI 423
>At2g34400.1 68415.m04215 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 617
Score = 27.5 bits (58), Expect = 6.5
Identities = 9/36 (25%), Positives = 18/36 (50%)
Frame = +1
Query: 451 IAKSSILLDVKPWDDETDMKEMENQVRTIEMEGLLW 558
+ S++L D+K WD+ M+ + ++ G W
Sbjct: 535 VISSNVLADMKMWDESAKMRALMRDRGVVKTPGCSW 570
>At2g36270.1 68415.m04452 bZIP transcription factor family protein /
ABA-responsive element-binding protein, putative similar
to ABA-responsive element binding protein 1 (AREB1)
GI:9967417 from [Arabidopsis thaliana]; contains a bZIP
transcription factor basic domain signature (PDOC00036)
Length = 442
Score = 27.1 bits (57), Expect = 8.7
Identities = 10/20 (50%), Positives = 12/20 (60%)
Frame = -3
Query: 318 CWGGSRGFSTGGQWAGALRP 259
C+GG GF GGQ G + P
Sbjct: 293 CYGGGVGFGAGGQQMGMVGP 312
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.316 0.131 0.386
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,114,818
Number of Sequences: 28952
Number of extensions: 205094
Number of successful extensions: 600
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 586
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 599
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1082538160
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.6 bits)
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