BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fmgV11o04r
(701 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBP8B7.03c |rpl402|rpl4-2, rpl4|60S ribosomal protein L2|Schizo... 159 4e-40
SPBC1711.06 |rpl401|rpl4-1, rpl4|60S ribosomal protein L2|Schizo... 158 6e-40
SPAC6G9.10c |sen1||ATP-dependent 5' to 3' DNA/RNA helicase Sen1|... 28 1.1
SPBC13E7.01 |cwf22|SPBC15D4.16|splicing factor Cwf22|Schizosacch... 28 1.1
SPBC13E7.10c |brf1|SPBC30D10.20|transcription factor TFIIIB comp... 25 7.9
SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr 1||... 25 7.9
SPBC12C2.05c |||diacylglycerol binding protein Bzz1 |Schizosacch... 25 7.9
>SPBP8B7.03c |rpl402|rpl4-2, rpl4|60S ribosomal protein
L2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 363
Score = 159 bits (386), Expect = 4e-40
Identities = 82/164 (50%), Positives = 110/164 (67%), Gaps = 2/164 (1%)
Frame = -3
Query: 699 KGKMRNRRRIQRKGPLIIFNKDQVLTRAFRNIPGVEXXXXXXXXXXXLAPGGHLGRFVIW 520
KGK+RNRR +QR+GPL++FN+D + +AFRNIPGVE LAPGGHLGRFVIW
Sbjct: 193 KGKLRNRRHVQRRGPLVVFNEDAGIVKAFRNIPGVEIVNVRRLNLLQLAPGGHLGRFVIW 252
Query: 519 TQSAFGRLDPLFGSWKTPSKQKKNFNLPQPKMANTDLTRLLKSDEIRKVLRAPN-KRVIR 343
T+SAFG LD +FGS ++ KKN+ LP+ ++N D+TRL+ SDEI+ +++A RV R
Sbjct: 253 TKSAFGLLDSVFGSTTEAAQLKKNYFLPENIISNADVTRLINSDEIQSIVKAAGPSRVKR 312
Query: 342 A-TRKLNPLTNNKAMLKLNPYAAVLKRKAILELRRRKNLKALAD 214
A +K NPL N + +LNPYA KA ++L K KA +
Sbjct: 313 AHVQKKNPLKNKAVLARLNPYAKAY--KANVKLNTGKTPKAAGE 354
>SPBC1711.06 |rpl401|rpl4-1, rpl4|60S ribosomal protein
L2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 363
Score = 158 bits (384), Expect = 6e-40
Identities = 81/164 (49%), Positives = 110/164 (67%), Gaps = 2/164 (1%)
Frame = -3
Query: 699 KGKMRNRRRIQRKGPLIIFNKDQVLTRAFRNIPGVEXXXXXXXXXXXLAPGGHLGRFVIW 520
KGK+RNRR +QR+GPL++FN+D + +AFRNIPGVE LAPGGHLGRFVIW
Sbjct: 193 KGKLRNRRHVQRRGPLVVFNEDTGIVKAFRNIPGVEIVNVRRLNLLQLAPGGHLGRFVIW 252
Query: 519 TQSAFGRLDPLFGSWKTPSKQKKNFNLPQPKMANTDLTRLLKSDEIRKVLRAPN-KRVIR 343
T+SAFG LD +FGS ++ KKN+ LP+ ++N D+TRL+ SDEI+ +++A RV R
Sbjct: 253 TKSAFGLLDSVFGSTTEVAQLKKNYFLPENIISNADVTRLINSDEIQSIVKAAGPSRVKR 312
Query: 342 A-TRKLNPLTNNKAMLKLNPYAAVLKRKAILELRRRKNLKALAD 214
A +K NPL N + +LNPYA KA +++ K KA +
Sbjct: 313 AHVQKKNPLKNKAVLSRLNPYAKAY--KANVKINSEKTPKAAGE 354
>SPAC6G9.10c |sen1||ATP-dependent 5' to 3' DNA/RNA helicase
Sen1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1687
Score = 28.3 bits (60), Expect = 1.1
Identities = 27/109 (24%), Positives = 46/109 (42%)
Frame = -3
Query: 462 KQKKNFNLPQPKMANTDLTRLLKSDEIRKVLRAPNKRVIRATRKLNPLTNNKAMLKLNPY 283
KQK + + Q K LT+ S E + VL +R T+K LTN+ ++K +P
Sbjct: 730 KQKAHLSADQCKQLANVLTQA--SPEAKTVLEQHRLSEMRKTKKQTELTNSAHVIKPSPT 787
Query: 282 AAVLKRKAILELRRRKNLKALADAEKSGLKLSKRNPAMKAEKLRERRRK 136
+ ++ + + L ++ L+KRN K + RK
Sbjct: 788 PQITVKQNTTKSSSAPRMGMLEQLKQE--YLTKRNFESKLKSSAVSSRK 834
>SPBC13E7.01 |cwf22|SPBC15D4.16|splicing factor
Cwf22|Schizosaccharomyces pombe|chr 2|||Manual
Length = 834
Score = 28.3 bits (60), Expect = 1.1
Identities = 19/90 (21%), Positives = 43/90 (47%), Gaps = 2/90 (2%)
Frame = -3
Query: 396 KSDEIRKVLRAPNKRVIRATRKLNPLTNNKAMLKLNPYAAVLKRKAILELRRRKNLKA-- 223
+S + + +K+ IR R+L+P ++ + N Y+ + + ++ R R
Sbjct: 659 RSRSVTPINNINHKKYIRKDRELSPRGRERSSNR-NSYSDLSRSSSLSRGRSRSYTPEGR 717
Query: 222 LADAEKSGLKLSKRNPAMKAEKLRERRRKN 133
L ++E G + +PA + + R+R R++
Sbjct: 718 LIESEDKGYRSRSSSPASRKYRSRQRYRRS 747
>SPBC13E7.10c |brf1|SPBC30D10.20|transcription factor TFIIIB complex
subunit Brf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 500
Score = 25.4 bits (53), Expect = 7.9
Identities = 24/93 (25%), Positives = 42/93 (45%), Gaps = 1/93 (1%)
Frame = -3
Query: 414 DLTRLLKSDEIRKVLRAPNKRVIRATRKLNPLTNNKAM-LKLNPYAAVLKRKAILELRRR 238
++T LK DE+RK+ N + L + +++ + L+ + K + +EL
Sbjct: 366 EVTETLKGDELRKISLQVNVKFSEEEVTLEDVDDDEIEDILLDKDEILTKTQVWMEL--- 422
Query: 237 KNLKALADAEKSGLKLSKRNPAMKAEKLRERRR 139
N LA+ E LKL + + R+RRR
Sbjct: 423 -NKDYLAEEEAKNLKLQEDLKKGIVRQPRKRRR 454
>SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1158
Score = 25.4 bits (53), Expect = 7.9
Identities = 17/54 (31%), Positives = 32/54 (59%)
Frame = +1
Query: 280 RVRIEFQHRLVVSERVQFACSTDHAFVGSTEDLPDLIRLEKTCEVSVGHLRLGQ 441
+V +EF+ RL + +RV+ AF+GS E + L+ + +T + ++ L LG+
Sbjct: 207 QVAVEFRKRLNIGDRVKDGLLYKDAFLGS-EAVDVLMHIVRTTDRNLA-LLLGR 258
>SPBC12C2.05c |||diacylglycerol binding protein Bzz1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 642
Score = 25.4 bits (53), Expect = 7.9
Identities = 13/45 (28%), Positives = 22/45 (48%)
Frame = +1
Query: 526 DETSKMTSRSQLQEVQLVNIQELHTGDVAESASQNLILVEDNEGS 660
+ET+ S++ V L + H G + S Q L+E ++GS
Sbjct: 507 EETTPAEPASKVDGVVLYDFTGEHEGVITASEGQEFTLLEPDDGS 551
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,334,690
Number of Sequences: 5004
Number of extensions: 42426
Number of successful extensions: 158
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 153
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 156
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 325165428
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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