BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fmgV11o02r (751 letters) Database: mosquito 2352 sequences; 563,979 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value AF313909-1|AAL99382.1| 1024|Anopheles gambiae collagen IV alpha ... 29 0.15 AJ302662-1|CAC35527.1| 76|Anopheles gambiae gSG9 protein protein. 26 1.1 AY027891-1|AAK15783.1| 801|Anopheles gambiae collagen IV alpha ... 25 1.9 AF457547-1|AAL68777.1| 163|Anopheles gambiae selenoprotein prot... 25 3.3 AF281078-1|AAF82131.1| 2051|Anopheles gambiae vitellogenin 1 pro... 24 5.8 >AF313909-1|AAL99382.1| 1024|Anopheles gambiae collagen IV alpha 1 chain protein. Length = 1024 Score = 29.1 bits (62), Expect = 0.15 Identities = 29/89 (32%), Positives = 39/89 (43%), Gaps = 2/89 (2%) Frame = -2 Query: 381 DRGFQGVMKRWGFKGMPASHGVTKTHRRPGNIGSGGEKARVWPGTKMP-GHMGNRWRT-L 208 DRG G+ G+ G G PG++G GE PG K P GH G R + Sbjct: 126 DRGDPGLPGSLGYPGEKGDLGTPGPPGYPGDVGPKGE-----PGPKGPAGHPGAPGRPGV 180 Query: 207 RGVKILRIDTKYNVIWTLGVAIPGETGAM 121 GVK L K ++ + +PG+ G M Sbjct: 181 DGVKGLP-GLKGDIGAPGVIGLPGQKGDM 208 Score = 27.1 bits (57), Expect = 0.62 Identities = 18/60 (30%), Positives = 25/60 (41%), Gaps = 4/60 (6%) Frame = -2 Query: 381 DRGFQGVMKRWGFKGMPASHGVTKTHRRPGNIGSGGEKARVWPGTK----MPGHMGNRWR 214 + G +G M G G+P G+ G++G GEK P K +PG G R Sbjct: 501 EAGAKGEMGIQGLPGLPGPAGLNGLPGMKGDMGPLGEKGDACPVVKGEKGLPGRPGKTGR 560 Score = 24.2 bits (50), Expect = 4.4 Identities = 14/43 (32%), Positives = 17/43 (39%) Frame = -2 Query: 381 DRGFQGVMKRWGFKGMPASHGVTKTHRRPGNIGSGGEKARVWP 253 DRG G+M R G G+P G G G G + P Sbjct: 598 DRGDSGLMGRPGNDGLPGPQGQRGLPGPQGEKGDQGPPGFIGP 640 >AJ302662-1|CAC35527.1| 76|Anopheles gambiae gSG9 protein protein. Length = 76 Score = 26.2 bits (55), Expect = 1.1 Identities = 11/32 (34%), Positives = 18/32 (56%) Frame = +1 Query: 208 QCSPSVTHVSRHFCSRPNSCFFTTRTNITRSS 303 QCSP +R + S+P+S +T N+ + S Sbjct: 22 QCSPFFFQYNRPYLSQPSSQLASTAANVVQRS 53 >AY027891-1|AAK15783.1| 801|Anopheles gambiae collagen IV alpha 1 chain precursor protein. Length = 801 Score = 25.4 bits (53), Expect = 1.9 Identities = 19/56 (33%), Positives = 27/56 (48%), Gaps = 2/56 (3%) Frame = -2 Query: 381 DRGFQGVMKRWGFKGMPASHGVTKTHRRPGNIGSGG-EKARVWPG-TKMPGHMGNR 220 DRG G+ G+ G+P ++GV PG G G PG + +PG+ G R Sbjct: 149 DRGRDGLP---GYPGIPGTNGVPGVPGAPGLAGRDGCNGTDGLPGLSGLPGNPGPR 201 Score = 25.4 bits (53), Expect = 1.9 Identities = 21/60 (35%), Positives = 28/60 (46%), Gaps = 1/60 (1%) Frame = -2 Query: 396 RSKTMDRGFQGVMKRWGFKGMPASHGVTKTHRRPGNIGSGGEKA-RVWPGTKMPGHMGNR 220 R + +G QGV R G +GMP G G++G G + R +PG PG G R Sbjct: 440 RGQMGPKGGQGVPGRPGPEGMPGDKG---DKGESGSVGMPGPQGPRGYPG--QPGPEGLR 494 Score = 25.0 bits (52), Expect = 2.5 Identities = 17/52 (32%), Positives = 25/52 (48%), Gaps = 8/52 (15%) Frame = -2 Query: 399 IRSKTMDRGFQGVMK--------RWGFKGMPASHGVTKTHRRPGNIGSGGEK 268 ++ + +RGF+GVM R G G+P G RPG G+ GE+ Sbjct: 519 LKGQKGERGFKGVMGTPGDAKEGRPGAPGLPGRDGEKGEPGRPGLPGAKGER 570 Score = 24.6 bits (51), Expect = 3.3 Identities = 18/43 (41%), Positives = 19/43 (44%) Frame = -2 Query: 354 RWGFKGMPASHGVTKTHRRPGNIGSGGEKARVWPGTKMPGHMG 226 R G KG G RPG G GE R +PG MPG G Sbjct: 584 RPGMKGDKGERGYAGEPGRPGASGVPGE--RGYPG--MPGEDG 622 Score = 24.2 bits (50), Expect = 4.4 Identities = 12/34 (35%), Positives = 13/34 (38%) Frame = -2 Query: 375 GFQGVMKRWGFKGMPASHGVTKTHRRPGNIGSGG 274 G GV G+ GMP G PG G G Sbjct: 604 GASGVPGERGYPGMPGEDGTPGLRGEPGPKGEPG 637 Score = 23.8 bits (49), Expect = 5.8 Identities = 18/53 (33%), Positives = 22/53 (41%), Gaps = 1/53 (1%) Frame = -2 Query: 381 DRGFQGVMKRWGFKGMPASHGVTKTHRRPGNIGSGGEKARVWPGTK-MPGHMG 226 +RG+ G R G G+P G G G GE PG K PG +G Sbjct: 593 ERGYAGEPGRPGASGVPGERGYPGMPGEDGTPGLRGE-----PGPKGEPGLLG 640 Score = 23.8 bits (49), Expect = 5.8 Identities = 16/51 (31%), Positives = 19/51 (37%) Frame = -2 Query: 375 GFQGVMKRWGFKGMPASHGVTKTHRRPGNIGSGGEKARVWPGTKMPGHMGN 223 G G+ G KG P G+ PG G G + G PG GN Sbjct: 691 GKMGLRGMKGDKGRPGEAGIDGAPGAPGKDGLPGRHGQTVKGE--PGLKGN 739 >AF457547-1|AAL68777.1| 163|Anopheles gambiae selenoprotein protein. Length = 163 Score = 24.6 bits (51), Expect = 3.3 Identities = 9/11 (81%), Positives = 9/11 (81%) Frame = -3 Query: 512 LECCQKGTSAD 480 LECCQK T AD Sbjct: 57 LECCQKDTEAD 67 >AF281078-1|AAF82131.1| 2051|Anopheles gambiae vitellogenin 1 protein. Length = 2051 Score = 23.8 bits (49), Expect = 5.8 Identities = 13/44 (29%), Positives = 22/44 (50%), Gaps = 1/44 (2%) Frame = +3 Query: 174 ILYQYG-ES*HHAVFSICYPCVQAFLFQAKLVLFHHQNQYYQVF 302 ++Y + E+ H+ F++ Y + + K VL H Q Y Q F Sbjct: 1295 LVYPFASETYHYHQFNLYYDAQRTSVKNVKFVLQHKQADYDQDF 1338 Database: mosquito Posted date: Oct 23, 2007 1:18 PM Number of letters in database: 563,979 Number of sequences in database: 2352 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 851,473 Number of Sequences: 2352 Number of extensions: 19496 Number of successful extensions: 54 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 37 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 54 length of database: 563,979 effective HSP length: 63 effective length of database: 415,803 effective search space used: 77339358 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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