Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fmgV11n09f
(543 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g37450.1 68417.m05301 arabinogalactan-protein (AGP18) identic... 31 0.50
At5g40440.1 68418.m04904 mitogen-activated protein kinase kinase... 29 2.0
At3g02880.1 68416.m00282 leucine-rich repeat transmembrane prote... 29 2.0
At1g74190.1 68414.m08592 leucine-rich repeat family protein cont... 28 3.5
At5g56620.1 68418.m07069 no apical meristem (NAM) family protein... 28 4.6
At4g28780.1 68417.m04115 GDSL-motif lipase/hydrolase family prot... 28 4.6
At2g19880.1 68415.m02324 ceramide glucosyltransferase, putative ... 28 4.6
At1g36925.1 68414.m04599 hypothetical protein 28 4.6
At1g17260.1 68414.m02102 ATPase 10, plasma membrane-type, putati... 27 6.1
At3g26720.1 68416.m03341 glycosyl hydrolase family 38 protein si... 27 8.1
At3g01180.1 68416.m00023 glycogen synthase, putative similar to ... 27 8.1
>At4g37450.1 68417.m05301 arabinogalactan-protein (AGP18) identical
to gi_11935088_gb_AAG41964
Length = 209
Score = 31.1 bits (67), Expect = 0.50
Identities = 21/65 (32%), Positives = 29/65 (44%)
Frame = +2
Query: 53 PCSQQLAASQPTTLALECLSVTAPVWLKLKSPWATFGSSSVLVPYSPTSISCLLPLVSLE 232
P S PTT + +VT+P K+P A+ SS V P SP +S P +
Sbjct: 28 PTKSPTTPSAPTTSPTKSPAVTSPTTAPAKTPTAS-ASSPVESPKSPAPVSESSPPPTPV 86
Query: 233 PSTGP 247
P + P
Sbjct: 87 PESSP 91
>At5g40440.1 68418.m04904 mitogen-activated protein kinase kinase
(MAPKK), putative (MKK3) similar to NPK2 [Nicotiana
tabacum] gi|862342|dbj|BAA06731; mitogen-activated
protein kinase kinase (MAPKK) family, PMID:12119167
Length = 520
Score = 29.1 bits (62), Expect = 2.0
Identities = 18/59 (30%), Positives = 30/59 (50%), Gaps = 6/59 (10%)
Frame = +1
Query: 154 NVWIQQCAGAVLTNFHILSVATC------FSGAFYRPDTRRIRLGSDIRNEGGLIVNVL 312
N++ ++ +LT L A C F GAFY PD+ +I + + N GG + ++L
Sbjct: 115 NIFEREKRQQLLTEIRTLCEAPCHEGLVDFHGAFYSPDSGQISIALEYMN-GGSLADIL 172
>At3g02880.1 68416.m00282 leucine-rich repeat transmembrane protein
kinase, putative contains Pfam profiles: PF00069
Eukaryotic protein kinase domain, PF00560 Leucine Rich
Repeat (5 copies)
Length = 627
Score = 29.1 bits (62), Expect = 2.0
Identities = 15/62 (24%), Positives = 29/62 (46%)
Frame = -1
Query: 294 AAFISYVRTETNTTSIGPVEGSRETSGNRQDMEVGEYGTSTLLDPNVAQGDFNLSQTGAV 115
A ISY+ + TTS G ++ S + + +V +YG + ++ A + + +
Sbjct: 453 ARAISYLHSRDGTTSHGNIKSSNILLSDSYEAKVSDYGLAPIISSTSAPNRIDGYRAPEI 512
Query: 114 TD 109
TD
Sbjct: 513 TD 514
>At1g74190.1 68414.m08592 leucine-rich repeat family protein
contains leucine rich-repeat (LRR) domains Pfam:PF00560,
INTERPRO:IPR001611; contains similarity to Cf-2.1
[Lycopersicon pimpinellifolium] gi|1184075|gb|AAC15779
Length = 965
Score = 28.3 bits (60), Expect = 3.5
Identities = 25/81 (30%), Positives = 38/81 (46%), Gaps = 1/81 (1%)
Frame = +1
Query: 196 FHILSVATCFSGAFYRPDTRRIRLGSDIRNEGGLIVNV-LLANNHHSFDPTNMASDISVV 372
FH LS AT + F R + GS E + N+ LL + + F+ + ++S +
Sbjct: 149 FHFLSAATSLTTLFLRSNNMD---GSFPAKELRDLTNLELLDLSRNRFNGSIPIQELSSL 205
Query: 373 RLSSAITLGGNIQQASVILQG 435
R A+ L GN S+ LQG
Sbjct: 206 RKLKALDLSGNEFSGSMELQG 226
>At5g56620.1 68418.m07069 no apical meristem (NAM) family protein
contains Pfam PF02365: No apical meristem (NAM) domain;
Length = 386
Score = 27.9 bits (59), Expect = 4.6
Identities = 12/27 (44%), Positives = 14/27 (51%), Gaps = 5/27 (18%)
Frame = -3
Query: 181 HQHTAGSKRCPG-----RLQLEPNWGG 116
H H GSK CPG + +PNW G
Sbjct: 16 HHHLCGSKHCPGCGRMIQAATKPNWVG 42
>At4g28780.1 68417.m04115 GDSL-motif lipase/hydrolase family protein
similar to family II lipase EXL3 (GI:15054386), EXL1
(GI:15054382), EXL2 (GI:15054384) [Arabidopsis
thaliana]; contains Pfam profile PF00657:
Lipase/Acylhydrolase with GDSL-like motif
Length = 367
Score = 27.9 bits (59), Expect = 4.6
Identities = 16/55 (29%), Positives = 26/55 (47%), Gaps = 1/55 (1%)
Frame = -2
Query: 494 PCGVFPQPMSSTLKRGGTCTP*RMTEACCILPPRVIAELRRTT-EISLAIFVGSN 333
P G P ++S+ G C P +A I P ++ L+ EI +F+G+N
Sbjct: 222 PLGCVPAELASSGSVNGECAP-EAQQAAAIFNPLLVQMLQGLNREIGSDVFIGAN 275
>At2g19880.1 68415.m02324 ceramide glucosyltransferase, putative
similar to ceramide glucosyltransferase (GI:14718995)
[Gossypium arboreum]; weak similarity to Ceramide
glucosyltransferase (Glucosylceramide synthase) (GCS)
(UDP-glucose:N-acylsphingosine D-glucosyltransferase)
(GLCT-1) (Swiss-Prot:Q16739) [Homo sapiens]
Length = 519
Score = 27.9 bits (59), Expect = 4.6
Identities = 10/20 (50%), Positives = 14/20 (70%)
Frame = -2
Query: 506 VTAPPCGVFPQPMSSTLKRG 447
+T+PP VFP P++S L G
Sbjct: 289 ITSPPVAVFPHPLASDLSFG 308
>At1g36925.1 68414.m04599 hypothetical protein
Length = 161
Score = 27.9 bits (59), Expect = 4.6
Identities = 14/40 (35%), Positives = 20/40 (50%), Gaps = 3/40 (7%)
Frame = -3
Query: 361 YRWPYSWDRMSDGYWQVT---HSRSSRLHFVCQNRDEYDE 251
++ PY + D Y + H+ SSR H N DEYD+
Sbjct: 109 HKEPYMQESRQDNYKEAPSKDHASSSRSHNSLYNTDEYDD 148
>At1g17260.1 68414.m02102 ATPase 10, plasma membrane-type, putative
/ proton pump 10, putative / proton-exporting ATPase,
putative strong similarity to SP|Q43128 ATPase 10,
plasma membrane-type (EC 3.6.3.6) (Proton pump 10)
{Arabidopsis thaliana}; contains InterPro accession
IPR001757: ATPase, E1-E2 type; contains Pfam profile
PF00690: Cation transporter/ATPase, N-terminus
Length = 947
Score = 27.5 bits (58), Expect = 6.1
Identities = 13/40 (32%), Positives = 22/40 (55%)
Frame = -1
Query: 174 TLLDPNVAQGDFNLSQTGAVTDRHSSAKVVG*DAASCCEQ 55
T+L +A G LSQ GA+T R ++ + + CC++
Sbjct: 299 TVLSVTLAIGSHRLSQQGAITKRMTAIEEMAGMDVLCCDK 338
>At3g26720.1 68416.m03341 glycosyl hydrolase family 38 protein
similar to lysosomal alpha-mannosidase GI:3522867 from
[Homo sapiens]
Length = 1019
Score = 27.1 bits (57), Expect = 8.1
Identities = 22/70 (31%), Positives = 33/70 (47%), Gaps = 1/70 (1%)
Frame = -1
Query: 288 FISYVRTETNTTSIGPVEGSRETSGN-RQDMEVGEYGTSTLLDPNVAQGDFNLSQTGAVT 112
F SYV ++T T+ G + S TSG+ Q++EVG+ + +LS VT
Sbjct: 568 FSSYVISDTGRTARG-LSASYVTSGSMNQNVEVGQGNLKLRYSEEGVKITRHLSTKNQVT 626
Query: 111 DRHSSAKVVG 82
S A +G
Sbjct: 627 AEQSYAYYIG 636
>At3g01180.1 68416.m00023 glycogen synthase, putative similar to
glycogen synthase Q43847 from [Solanum tuberosum]
Length = 792
Score = 27.1 bits (57), Expect = 8.1
Identities = 13/34 (38%), Positives = 20/34 (58%)
Frame = +2
Query: 146 PWATFGSSSVLVPYSPTSISCLLPLVSLEPSTGP 247
PW++ +SSV PY P+S+ P + +P T P
Sbjct: 204 PWSSVVASSVDPPYKPSSV-MTSPEKTSDPVTSP 236
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,719,502
Number of Sequences: 28952
Number of extensions: 284755
Number of successful extensions: 728
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 718
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 728
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1013649368
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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