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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fmgV11n05f
         (604 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_45114| Best HMM Match : RasGEF (HMM E-Value=3.7e-24)                29   2.9  
SB_40345| Best HMM Match : No HMM Matches (HMM E-Value=.)              29   2.9  
SB_33848| Best HMM Match : RasGEF_N (HMM E-Value=2.3e-13)              29   2.9  
SB_17923| Best HMM Match : No HMM Matches (HMM E-Value=.)              28   6.7  
SB_216| Best HMM Match : No HMM Matches (HMM E-Value=.)                28   6.7  
SB_43678| Best HMM Match : Ribonuclease_BN (HMM E-Value=0.81)          27   8.8  
SB_19245| Best HMM Match : ADK (HMM E-Value=0)                         27   8.8  

>SB_45114| Best HMM Match : RasGEF (HMM E-Value=3.7e-24)
          Length = 315

 Score = 29.1 bits (62), Expect = 2.9
 Identities = 18/60 (30%), Positives = 29/60 (48%), Gaps = 4/60 (6%)
 Frame = +2

Query: 353 FAASLADDLGMKHFPEANMDLHYIRPNGVDLRSFD----DQVPEDTRTFDHVNFNQNPNH 520
           FAA++   LG+K   +  M++       VD  SFD     Q+ E     +H+ F++ P H
Sbjct: 32  FAAAIIRTLGIKQGQKGPMEMDLAPDEQVDPNSFDKISSSQLAEQLTFVEHLLFSRVPAH 91


>SB_40345| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 1250

 Score = 29.1 bits (62), Expect = 2.9
 Identities = 10/29 (34%), Positives = 20/29 (68%)
 Frame = +2

Query: 302 NSKVIIVEGPVAAGKTAFAASLADDLGMK 388
           NS +++V GPV  GKT+  +++  +L ++
Sbjct: 440 NSDLVLVTGPVGCGKTSLLSAILGELSVR 468


>SB_33848| Best HMM Match : RasGEF_N (HMM E-Value=2.3e-13)
          Length = 899

 Score = 29.1 bits (62), Expect = 2.9
 Identities = 18/60 (30%), Positives = 29/60 (48%), Gaps = 4/60 (6%)
 Frame = +2

Query: 353 FAASLADDLGMKHFPEANMDLHYIRPNGVDLRSFD----DQVPEDTRTFDHVNFNQNPNH 520
           FAA++   LG+K   +  M++       VD  SFD     Q+ E     +H+ F++ P H
Sbjct: 812 FAAAIIRTLGIKQGQKGPMEMDLAPDEQVDPNSFDKISSSQLAEQLTFVEHLLFSRVPAH 871


>SB_17923| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 283

 Score = 27.9 bits (59), Expect = 6.7
 Identities = 11/20 (55%), Positives = 14/20 (70%)
 Frame = +2

Query: 371 DDLGMKHFPEANMDLHYIRP 430
           D+ G+ H PEA   L+YIRP
Sbjct: 201 DEEGIPHKPEAQKHLYYIRP 220


>SB_216| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 1315

 Score = 27.9 bits (59), Expect = 6.7
 Identities = 10/29 (34%), Positives = 20/29 (68%)
 Frame = +2

Query: 302 NSKVIIVEGPVAAGKTAFAASLADDLGMK 388
           +++V++V GPV  GKTA   ++  ++ +K
Sbjct: 506 SNQVVMVTGPVGCGKTALLMAILKEIPLK 534


>SB_43678| Best HMM Match : Ribonuclease_BN (HMM E-Value=0.81)
          Length = 468

 Score = 27.5 bits (58), Expect = 8.8
 Identities = 14/57 (24%), Positives = 29/57 (50%), Gaps = 2/57 (3%)
 Frame = +3

Query: 372 MTWV*SISPKLTWICTI--FVRTAST*GPSTTKYPRTLGPLTMLISIRILTIAWLQT 536
           +TWV ++S  +TW+ T+   V +  T   S T+       +T ++++    + W+ T
Sbjct: 221 VTWVVTVSSSVTWVVTVSSSVTSVVTVSSSVTRVVTVSFSVTWVVTVS-FNVTWVVT 276


>SB_19245| Best HMM Match : ADK (HMM E-Value=0)
          Length = 188

 Score = 27.5 bits (58), Expect = 8.8
 Identities = 12/43 (27%), Positives = 22/43 (51%)
 Frame = +2

Query: 317 IVEGPVAAGKTAFAASLADDLGMKHFPEANMDLHYIRPNGVDL 445
           I+ GP  +GK   +  +  D G++H     +  H+++ N  DL
Sbjct: 35  IIMGPPGSGKGTVSERIVRDFGVEHLSSGELLRHHVQ-NHTDL 76


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,365,012
Number of Sequences: 59808
Number of extensions: 368912
Number of successful extensions: 977
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 884
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 970
length of database: 16,821,457
effective HSP length: 79
effective length of database: 12,096,625
effective search space used: 1463691625
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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