BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fmgV11n05f (604 letters) Database: nematostella 59,808 sequences; 16,821,457 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SB_45114| Best HMM Match : RasGEF (HMM E-Value=3.7e-24) 29 2.9 SB_40345| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 2.9 SB_33848| Best HMM Match : RasGEF_N (HMM E-Value=2.3e-13) 29 2.9 SB_17923| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 6.7 SB_216| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 6.7 SB_43678| Best HMM Match : Ribonuclease_BN (HMM E-Value=0.81) 27 8.8 SB_19245| Best HMM Match : ADK (HMM E-Value=0) 27 8.8 >SB_45114| Best HMM Match : RasGEF (HMM E-Value=3.7e-24) Length = 315 Score = 29.1 bits (62), Expect = 2.9 Identities = 18/60 (30%), Positives = 29/60 (48%), Gaps = 4/60 (6%) Frame = +2 Query: 353 FAASLADDLGMKHFPEANMDLHYIRPNGVDLRSFD----DQVPEDTRTFDHVNFNQNPNH 520 FAA++ LG+K + M++ VD SFD Q+ E +H+ F++ P H Sbjct: 32 FAAAIIRTLGIKQGQKGPMEMDLAPDEQVDPNSFDKISSSQLAEQLTFVEHLLFSRVPAH 91 >SB_40345| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 1250 Score = 29.1 bits (62), Expect = 2.9 Identities = 10/29 (34%), Positives = 20/29 (68%) Frame = +2 Query: 302 NSKVIIVEGPVAAGKTAFAASLADDLGMK 388 NS +++V GPV GKT+ +++ +L ++ Sbjct: 440 NSDLVLVTGPVGCGKTSLLSAILGELSVR 468 >SB_33848| Best HMM Match : RasGEF_N (HMM E-Value=2.3e-13) Length = 899 Score = 29.1 bits (62), Expect = 2.9 Identities = 18/60 (30%), Positives = 29/60 (48%), Gaps = 4/60 (6%) Frame = +2 Query: 353 FAASLADDLGMKHFPEANMDLHYIRPNGVDLRSFD----DQVPEDTRTFDHVNFNQNPNH 520 FAA++ LG+K + M++ VD SFD Q+ E +H+ F++ P H Sbjct: 812 FAAAIIRTLGIKQGQKGPMEMDLAPDEQVDPNSFDKISSSQLAEQLTFVEHLLFSRVPAH 871 >SB_17923| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 283 Score = 27.9 bits (59), Expect = 6.7 Identities = 11/20 (55%), Positives = 14/20 (70%) Frame = +2 Query: 371 DDLGMKHFPEANMDLHYIRP 430 D+ G+ H PEA L+YIRP Sbjct: 201 DEEGIPHKPEAQKHLYYIRP 220 >SB_216| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 1315 Score = 27.9 bits (59), Expect = 6.7 Identities = 10/29 (34%), Positives = 20/29 (68%) Frame = +2 Query: 302 NSKVIIVEGPVAAGKTAFAASLADDLGMK 388 +++V++V GPV GKTA ++ ++ +K Sbjct: 506 SNQVVMVTGPVGCGKTALLMAILKEIPLK 534 >SB_43678| Best HMM Match : Ribonuclease_BN (HMM E-Value=0.81) Length = 468 Score = 27.5 bits (58), Expect = 8.8 Identities = 14/57 (24%), Positives = 29/57 (50%), Gaps = 2/57 (3%) Frame = +3 Query: 372 MTWV*SISPKLTWICTI--FVRTAST*GPSTTKYPRTLGPLTMLISIRILTIAWLQT 536 +TWV ++S +TW+ T+ V + T S T+ +T ++++ + W+ T Sbjct: 221 VTWVVTVSSSVTWVVTVSSSVTSVVTVSSSVTRVVTVSFSVTWVVTVS-FNVTWVVT 276 >SB_19245| Best HMM Match : ADK (HMM E-Value=0) Length = 188 Score = 27.5 bits (58), Expect = 8.8 Identities = 12/43 (27%), Positives = 22/43 (51%) Frame = +2 Query: 317 IVEGPVAAGKTAFAASLADDLGMKHFPEANMDLHYIRPNGVDL 445 I+ GP +GK + + D G++H + H+++ N DL Sbjct: 35 IIMGPPGSGKGTVSERIVRDFGVEHLSSGELLRHHVQ-NHTDL 76 Database: nematostella Posted date: Oct 22, 2007 1:22 PM Number of letters in database: 16,821,457 Number of sequences in database: 59,808 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 18,365,012 Number of Sequences: 59808 Number of extensions: 368912 Number of successful extensions: 977 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 884 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 970 length of database: 16,821,457 effective HSP length: 79 effective length of database: 12,096,625 effective search space used: 1463691625 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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