Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fmgV11m22r
(700 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, p... 217 4e-57
At1g06220.2 68414.m00656 elongation factor Tu family protein sim... 156 2e-38
At1g06220.1 68414.m00655 elongation factor Tu family protein sim... 156 2e-38
At5g25230.1 68418.m02991 elongation factor Tu family protein tra... 153 8e-38
At3g22980.1 68416.m02898 elongation factor Tu family protein sim... 93 2e-19
At2g45030.1 68415.m05606 mitochondrial elongation factor, putati... 52 5e-07
At1g45332.1 68414.m05195 mitochondrial elongation factor, putati... 52 5e-07
At1g62750.1 68414.m07082 elongation factor Tu family protein sim... 48 5e-06
At5g13650.2 68418.m01585 elongation factor family protein contai... 42 5e-04
At5g13650.1 68418.m01584 elongation factor family protein contai... 42 5e-04
At5g39900.1 68418.m04839 GTP-binding protein LepA, putative GTP-... 31 0.97
At5g61930.1 68418.m07774 expressed protein contains Pfam PF05634... 29 3.0
At5g29624.1 68418.m03640 DC1 domain-containing protein contains ... 29 3.9
At2g35490.1 68415.m04347 plastid-lipid associated protein PAP, p... 28 6.8
At4g23820.1 68417.m03425 glycoside hydrolase family 28 protein /... 27 9.0
At3g63180.1 68416.m07097 expressed protein 27 9.0
>At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2,
putative similar to ELONGATION FACTOR 2 GB:O14460 from
[Schizosaccharomyces pombe]
Length = 843
Score = 217 bits (531), Expect = 4e-57
Identities = 95/152 (62%), Positives = 122/152 (80%)
Frame = -1
Query: 700 LRGVRFNIYDVTLHTDAIHRGGGQIIPTTRRCLYACLLTAQPRLMEPVYLCEIQCPEVAV 521
+RG+ F + DV LH+DAIHRGGGQ+IPT RR +YA +TA+PRL+EPVY+ EIQ PE A+
Sbjct: 682 MRGICFEVCDVVLHSDAIHRGGGQVIPTARRVIYASQITAKPRLLEPVYMVEIQAPEGAL 741
Query: 520 GGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDH 341
GGIY VLN++RGHVFEE Q GTP++ +KAYLPV ESFGF++ LR+ T GQAFPQCVFDH
Sbjct: 742 GGIYSVLNQKRGHVFEEMQRPGTPLYNIKAYLPVVESFGFSSQLRAATSGQAFPQCVFDH 801
Query: 340 WQVLPGDPCEPQSKPYNVVQETRKRKGLKEGL 245
W+++ DP EP ++ +V + RKRKGLKE +
Sbjct: 802 WEMMSSDPLEPGTQASVLVADIRKRKGLKEAM 833
>At1g06220.2 68414.m00656 elongation factor Tu family protein similar
to Cryptosporidium parvum elongation factor-2 GB:U21667
GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 156 bits (378), Expect = 2e-38
Identities = 81/162 (50%), Positives = 101/162 (62%), Gaps = 12/162 (7%)
Frame = -1
Query: 700 LRGVRFNIYDVTLHTDAIHRGGGQIIPTTRRCLYACLLTAQPRLMEPVYLCEIQCPEVAV 521
+R V+F I D + + +HRG GQ+IPT RR Y+ L A PRLMEPVY EIQ P V
Sbjct: 798 IRNVKFKIVDARIAPEPLHRGSGQMIPTARRVAYSAFLMATPRLMEPVYYVEIQTPIDCV 857
Query: 520 GGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDH 341
IY VL+RRRGHV + GTP +IVKA+LPV ESFGF DLR +T GQAF VFDH
Sbjct: 858 TAIYTVLSRRRGHVTSDVPQPGTPAYIVKAFLPVIESFGFETDLRYHTQGQAFCLSVFDH 917
Query: 340 WQVLPGDPCEP--QSKPY----------NVVQETRKRKGLKE 251
W ++PGDP + Q +P + +TR+RKG+ E
Sbjct: 918 WAIVPGDPLDKAIQLRPLEPAPIQHLAREFMVKTRRRKGMSE 959
>At1g06220.1 68414.m00655 elongation factor Tu family protein similar
to Cryptosporidium parvum elongation factor-2 GB:U21667
GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 156 bits (378), Expect = 2e-38
Identities = 81/162 (50%), Positives = 101/162 (62%), Gaps = 12/162 (7%)
Frame = -1
Query: 700 LRGVRFNIYDVTLHTDAIHRGGGQIIPTTRRCLYACLLTAQPRLMEPVYLCEIQCPEVAV 521
+R V+F I D + + +HRG GQ+IPT RR Y+ L A PRLMEPVY EIQ P V
Sbjct: 798 IRNVKFKIVDARIAPEPLHRGSGQMIPTARRVAYSAFLMATPRLMEPVYYVEIQTPIDCV 857
Query: 520 GGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDH 341
IY VL+RRRGHV + GTP +IVKA+LPV ESFGF DLR +T GQAF VFDH
Sbjct: 858 TAIYTVLSRRRGHVTSDVPQPGTPAYIVKAFLPVIESFGFETDLRYHTQGQAFCLSVFDH 917
Query: 340 WQVLPGDPCEP--QSKPY----------NVVQETRKRKGLKE 251
W ++PGDP + Q +P + +TR+RKG+ E
Sbjct: 918 WAIVPGDPLDKAIQLRPLEPAPIQHLAREFMVKTRRRKGMSE 959
>At5g25230.1 68418.m02991 elongation factor Tu family protein
translation Elongation Factor 2, Schizosaccharomyces
pombe, PIR:T39902
Length = 973
Score = 153 bits (372), Expect = 8e-38
Identities = 80/162 (49%), Positives = 101/162 (62%), Gaps = 12/162 (7%)
Frame = -1
Query: 700 LRGVRFNIYDVTLHTDAIHRGGGQIIPTTRRCLYACLLTAQPRLMEPVYLCEIQCPEVAV 521
+R V+F I D + + +HRG GQ+IPT RR Y+ L A PRLMEPVY EIQ P V
Sbjct: 784 IRNVKFKIVDARIAPEPLHRGSGQMIPTARRVAYSAFLMATPRLMEPVYYVEIQTPIDCV 843
Query: 520 GGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDH 341
IY VL+RRRG+V + GTP +IVKA+LPV ESFGF DLR +T GQAF VFDH
Sbjct: 844 TAIYTVLSRRRGYVTSDVPQPGTPAYIVKAFLPVIESFGFETDLRYHTQGQAFCLSVFDH 903
Query: 340 WQVLPGDPCEP--QSKPY----------NVVQETRKRKGLKE 251
W ++PGDP + Q +P + +TR+RKG+ E
Sbjct: 904 WAIVPGDPLDKAIQLRPLEPAPIQHLAREFMVKTRRRKGMSE 945
>At3g22980.1 68416.m02898 elongation factor Tu family protein similar
to eukaryotic translation elongation factor 2
GB:NP_001952 [Homo sapiens]
Length = 1015
Score = 93.1 bits (221), Expect = 2e-19
Identities = 41/106 (38%), Positives = 61/106 (57%)
Frame = -1
Query: 634 GQIIPTTRRCLYACLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAG 455
GQ++ + A +L PR++E +Y CE+ +G +Y VL+RRR + +E G
Sbjct: 853 GQVMTAVKDACRAAVLQTNPRIVEAMYFCELNTAPEYLGPMYAVLSRRRARILKEEMQEG 912
Query: 454 TPMFIVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQVLPGDP 317
+ +F V AY+PV+ESFGF +LR T G A V HW++L DP
Sbjct: 913 SSLFTVHAYVPVSESFGFADELRKGTSGGASALMVLSHWEMLEEDP 958
>At2g45030.1 68415.m05606 mitochondrial elongation factor, putative
similar to SP|P25039 Elongation factor G 1,
mitochondrial precursor (mEF-G-1) {Saccharomyces
cerevisiae}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03764: Elongation factor
G domain IV, PF00679: Elongation factor G C-terminus
Length = 754
Score = 51.6 bits (118), Expect = 5e-07
Identities = 25/76 (32%), Positives = 42/76 (55%)
Frame = -1
Query: 595 CLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVN 416
C A+P ++EPV L E++ P G + G +N+R+G + Q ++ A +P+N
Sbjct: 649 CYTAARPVILEPVMLVELKVPTEFQGTVAGDINKRKGIIVGNDQEGDDS--VITANVPLN 706
Query: 415 ESFGFTADLRSNTGGQ 368
FG++ LRS T G+
Sbjct: 707 NMFGYSTSLRSMTQGK 722
>At1g45332.1 68414.m05195 mitochondrial elongation factor, putative
similar to mitochondrial elongation factor GI:3917 from
[Saccharomyces cerevisiae]
Length = 754
Score = 51.6 bits (118), Expect = 5e-07
Identities = 25/76 (32%), Positives = 42/76 (55%)
Frame = -1
Query: 595 CLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVN 416
C A+P ++EPV L E++ P G + G +N+R+G + Q ++ A +P+N
Sbjct: 649 CYTAARPVILEPVMLVELKVPTEFQGTVAGDINKRKGIIVGNDQEGDDS--VITANVPLN 706
Query: 415 ESFGFTADLRSNTGGQ 368
FG++ LRS T G+
Sbjct: 707 NMFGYSTSLRSMTQGK 722
>At1g62750.1 68414.m07082 elongation factor Tu family protein
similar to elongation factor G SP:P34811 [Glycine max
(Soybean)]
Length = 783
Score = 48.4 bits (110), Expect = 5e-06
Identities = 27/86 (31%), Positives = 44/86 (51%)
Frame = -1
Query: 583 AQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFG 404
A PR++EP+ E+ PE +G + G LN RRG + G + +V + +P+ E F
Sbjct: 683 AGPRMLEPIMRVEVVTPEEHLGDVIGDLNSRRGQINSFGDKPG-GLKVVDSLVPLAEMFQ 741
Query: 403 FTADLRSNTGGQAFPQCVFDHWQVLP 326
+ + LR T G+A + V+P
Sbjct: 742 YVSTLRGMTKGRASYTMQLAKFDVVP 767
>At5g13650.2 68418.m01585 elongation factor family protein contains
Pfam profiles: PF00009 elongation factor Tu GTP binding
domain,PF00679 elongation factor G C-terminus, PF03144
elongation factor Tu domain 2
Length = 676
Score = 41.5 bits (93), Expect = 5e-04
Identities = 21/85 (24%), Positives = 39/85 (45%)
Frame = -1
Query: 574 RLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTA 395
+L+EP + ++ PE +G + +L +RRG +F+ V ++ +P G
Sbjct: 473 KLLEPYEIATVEVPEAHMGPVVELLGKRRGQMFDMQGVGSEGTTFLRYKIPTRGLLGLRN 532
Query: 394 DLRSNTGGQAFPQCVFDHWQVLPGD 320
+ + + G A VFD + GD
Sbjct: 533 AILTASRGTAILNTVFDSYGPWAGD 557
>At5g13650.1 68418.m01584 elongation factor family protein contains
Pfam profiles: PF00009 elongation factor Tu GTP binding
domain,PF00679 elongation factor G C-terminus, PF03144
elongation factor Tu domain 2
Length = 675
Score = 41.5 bits (93), Expect = 5e-04
Identities = 21/85 (24%), Positives = 39/85 (45%)
Frame = -1
Query: 574 RLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTA 395
+L+EP + ++ PE +G + +L +RRG +F+ V ++ +P G
Sbjct: 472 KLLEPYEIATVEVPEAHMGPVVELLGKRRGQMFDMQGVGSEGTTFLRYKIPTRGLLGLRN 531
Query: 394 DLRSNTGGQAFPQCVFDHWQVLPGD 320
+ + + G A VFD + GD
Sbjct: 532 AILTASRGTAILNTVFDSYGPWAGD 556
>At5g39900.1 68418.m04839 GTP-binding protein LepA, putative
GTP-binding protein GUF1 - Saccharomyces cerevisiae,
PIR:S50374
Length = 661
Score = 30.7 bits (66), Expect = 0.97
Identities = 21/68 (30%), Positives = 32/68 (47%), Gaps = 1/68 (1%)
Frame = -1
Query: 565 EPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNE-SFGFTADL 389
EP + I P VG + + + RRG E + + +F+ K LP+ E F +L
Sbjct: 466 EPTVIATIILPSEYVGAVINLCSDRRGQQLEYTFIDAQRVFL-KYQLPLREIVVDFYDEL 524
Query: 388 RSNTGGQA 365
+S T G A
Sbjct: 525 KSITSGYA 532
>At5g61930.1 68418.m07774 expressed protein contains Pfam PF05634:
Arabidopsis thaliana protein of unknown function
(DUF794)
Length = 340
Score = 29.1 bits (62), Expect = 3.0
Identities = 11/17 (64%), Positives = 12/17 (70%)
Frame = +1
Query: 280 PVQRCRACSEVRTGLQG 330
PV RCR C+EV G QG
Sbjct: 62 PVHRCRLCAEVHIGKQG 78
>At5g29624.1 68418.m03640 DC1 domain-containing protein contains
Pfam PF03107: DC1 domain
Length = 488
Score = 28.7 bits (61), Expect = 3.9
Identities = 19/80 (23%), Positives = 27/80 (33%)
Frame = +2
Query: 449 RCTCHLGLFENXXXXXXXXXXXXXHSYFRTLNFTKIYRLHKTGLSS*QTCVQASSSCWND 628
RC C + H L K + LHK L+ TC+ + C
Sbjct: 309 RCQCCILAINGDPCYRCVECDFILHDACANLPRKKQHLLHKHKLTLSSTCIDGETYCQCK 368
Query: 629 LATTSMDGISMECYIIDVES 688
+ DG +C+ D ES
Sbjct: 369 VYDVYTDGFIYKCHHEDCES 388
>At2g35490.1 68415.m04347 plastid-lipid associated protein PAP,
putative similar to plastid-lipid associated protein
PAP3 [Brassica rapa] GI:14248552; contains Pfam profile
PF04755: PAP_fibrillin
Length = 376
Score = 27.9 bits (59), Expect = 6.8
Identities = 12/22 (54%), Positives = 13/22 (59%)
Frame = -1
Query: 352 VFDHWQVLPGDPCEPQSKPYNV 287
V D W PGD EP S+P NV
Sbjct: 71 VTDEWGEKPGDANEPDSQPDNV 92
>At4g23820.1 68417.m03425 glycoside hydrolase family 28 protein /
polygalacturonase (pectinase) family protein weak
similarity to polygalacturonase PG1 [Glycine max]
GI:5669846; contains PF00295: Glycosyl hydrolases family
28
Length = 444
Score = 27.5 bits (58), Expect = 9.0
Identities = 11/22 (50%), Positives = 17/22 (77%), Gaps = 1/22 (4%)
Frame = -3
Query: 491 SWSRFRRVPGGRY-TYVHCEGL 429
S+ R R +PGGRY +++H +GL
Sbjct: 123 SYGRGRELPGGRYMSFIHGDGL 144
>At3g63180.1 68416.m07097 expressed protein
Length = 978
Score = 27.5 bits (58), Expect = 9.0
Identities = 10/35 (28%), Positives = 21/35 (60%)
Frame = -2
Query: 432 PTYLSMSRSVLLPICVPTPADRPSRSAYSTIGRSS 328
P ++ + L P+ VP+ + RP++S + +G +S
Sbjct: 539 PAFIYPANHHLQPVMVPSKSSRPTKSPHLAVGLAS 573
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,027,922
Number of Sequences: 28952
Number of extensions: 344971
Number of successful extensions: 830
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 801
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 825
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1496852856
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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