BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fmgV11m03r (700 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 267 1e-72 SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 267 1e-72 SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyce... 182 3e-47 SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma... 101 1e-22 SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 46 4e-06 SPAC227.18 |lys3|SPAC2F7.01|saccharopine dehydrogenase [NAD+, L-... 26 4.5 SPCP31B10.05 |||tyrosyl-DNA phosphodiesterase |Schizosaccharomyc... 26 4.5 SPAC227.16c |||GINS complex subunit Psf3|Schizosaccharomyces pom... 26 4.5 SPAC1006.08 |etd1||ethanol-hypersensitive mutant protein |Schizo... 26 6.0 SPCC550.05 |nse1||Smc5-6 complex non-SMC subunit 1|Schizosacchar... 25 7.9 SPBC1734.15 |rsc4|brd1|RSC complex subunit Rsc4|Schizosaccharomy... 25 7.9 SPAC23H4.16c |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 25 7.9 SPAC11D3.15 |||oxoprolinase |Schizosaccharomyces pombe|chr 1|||M... 25 7.9 >SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosaccharomyces pombe|chr 3|||Manual Length = 842 Score = 267 bits (654), Expect = 1e-72 Identities = 124/184 (67%), Positives = 140/184 (76%) Frame = -2 Query: 696 SKGVQYLNEIKDSVVAGFQWAAKEGVMAEENLRGVRFNIYDVTLHTDAIHRGGGQIIPTT 517 +K V YLNEIKDSVVA F WA+KEG M EENLR RFNI DV LH DAIHRGGGQIIPT Sbjct: 650 TKAVAYLNEIKDSVVAAFAWASKEGPMFEENLRSCRFNILDVVLHADAIHRGGGQIIPTA 709 Query: 516 RRCLYACLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVK 337 RR +YA L A P + EPV+L EIQ E A+GGIY VLN++RGHVF E Q GTP++ +K Sbjct: 710 RRVVYASTLLASPIIQEPVFLVEIQVSENAMGGIYSVLNKKRGHVFSEEQRVGTPLYNIK 769 Query: 336 AYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQVLPGDPCEPQSKPYNVVQETRKRKGLK 157 AYLPVNESFGFT +LR T GQAFPQ VFDHW + GDP +P SKP +V E RKRKGLK Sbjct: 770 AYLPVNESFGFTGELRQATAGQAFPQLVFDHWSPMSGDPLDPTSKPGQIVCEARKRKGLK 829 Query: 156 EGLP 145 E +P Sbjct: 830 ENVP 833 >SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elongation factor 2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 842 Score = 267 bits (654), Expect = 1e-72 Identities = 124/184 (67%), Positives = 140/184 (76%) Frame = -2 Query: 696 SKGVQYLNEIKDSVVAGFQWAAKEGVMAEENLRGVRFNIYDVTLHTDAIHRGGGQIIPTT 517 +K V YLNEIKDSVVA F WA+KEG M EENLR RFNI DV LH DAIHRGGGQIIPT Sbjct: 650 TKAVAYLNEIKDSVVAAFAWASKEGPMFEENLRSCRFNILDVVLHADAIHRGGGQIIPTA 709 Query: 516 RRCLYACLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVK 337 RR +YA L A P + EPV+L EIQ E A+GGIY VLN++RGHVF E Q GTP++ +K Sbjct: 710 RRVVYASTLLASPIIQEPVFLVEIQVSENAMGGIYSVLNKKRGHVFSEEQRVGTPLYNIK 769 Query: 336 AYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQVLPGDPCEPQSKPYNVVQETRKRKGLK 157 AYLPVNESFGFT +LR T GQAFPQ VFDHW + GDP +P SKP +V E RKRKGLK Sbjct: 770 AYLPVNESFGFTGELRQATAGQAFPQLVFDHWSPMSGDPLDPTSKPGQIVCEARKRKGLK 829 Query: 156 EGLP 145 E +P Sbjct: 830 ENVP 833 >SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyces pombe|chr 2|||Manual Length = 983 Score = 182 bits (444), Expect = 3e-47 Identities = 81/160 (50%), Positives = 109/160 (68%) Frame = -2 Query: 678 LNEIKDSVVAGFQWAAKEGVMAEENLRGVRFNIYDVTLHTDAIHRGGGQIIPTTRRCLYA 499 LN +K+ + GFQW +EG + +E +R V F + DV L + I+RGGGQIIPT RR Y+ Sbjct: 772 LNSVKEYIKQGFQWGTREGPLCDETIRNVNFRLMDVVLAPEQIYRGGGQIIPTARRVCYS 831 Query: 498 CLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVN 319 LTA PRLMEPVY+ E+ P ++ IY +L RRRGHV ++ G+P+++V+A +PV Sbjct: 832 SFLTASPRLMEPVYMVEVHAPADSLPIIYDLLTRRRGHVLQDIPRPGSPLYLVRALIPVI 891 Query: 318 ESFGFTADLRSNTGGQAFPQCVFDHWQVLPGDPCEPQSKP 199 +S GF DLR +T GQA Q VFDHWQV+PGDP + KP Sbjct: 892 DSCGFETDLRVHTQGQAMCQMVFDHWQVVPGDPLDKSIKP 931 >SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Manual Length = 1000 Score = 101 bits (241), Expect = 1e-22 Identities = 53/161 (32%), Positives = 88/161 (54%), Gaps = 9/161 (5%) Frame = -2 Query: 675 NEIKDSVVAGFQWAAKEGVMAEENLRGV-----RFNIYDVTLHTDAIHRGG----GQIIP 523 +++ + VV FQ +G + E ++G+ +F+I D + + + GQ+I Sbjct: 786 SDLSEYVVTAFQLITHQGPLCAEPVQGICVSIDQFDISDDSEDSKLLTINNPQIPGQVIS 845 Query: 522 TTRRCLYACLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFI 343 + + L PRLM +Y C++Q +G +YGV+++RRG V +E GTP FI Sbjct: 846 VVKESIRHGFLGWSPRLMLAMYSCDVQATSEVLGRVYGVVSKRRGRVIDEEMKEGTPFFI 905 Query: 342 VKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQVLPGDP 220 VKA +PV ESFGF ++ T G A+PQ +F +++L +P Sbjct: 906 VKALIPVVESFGFAVEILKRTSGAAYPQLIFHGFEMLDENP 946 >SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schizosaccharomyces pombe|chr 2|||Manual Length = 770 Score = 46.4 bits (105), Expect = 4e-06 Identities = 30/125 (24%), Positives = 56/125 (44%) Frame = -2 Query: 645 FQWAAKEGVMAEENLRGVRFNIYDVTLHTDAIHRGGGQIIPTTRRCLYACLLTAQPRLME 466 F A K+G + ++ RF + D H ++ T L A P ++E Sbjct: 619 FYEALKKGFLIGHPIKNCRFVLEDGAYHPVDSSELAFRL--ATISAFRTAFLQANPMVLE 676 Query: 465 PVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRS 286 P+ I P GG+ G L++R+ + + F ++A +P+N F +++D+R+ Sbjct: 677 PIMNVSITAPVEHQGGVIGNLDKRKATIVDSD--TDEDEFTLQAEVPLNSMFSYSSDIRA 734 Query: 285 NTGGQ 271 T G+ Sbjct: 735 LTKGK 739 >SPAC227.18 |lys3|SPAC2F7.01|saccharopine dehydrogenase [NAD+, L-lysine forming] |Schizosaccharomyces pombe|chr 1|||Manual Length = 368 Score = 26.2 bits (55), Expect = 4.5 Identities = 14/40 (35%), Positives = 22/40 (55%) Frame = +2 Query: 518 VVGMIWPPPLWMASVWSVTS*MLNLTPRKFSSAITPSLAA 637 V G+ PPPL + S+ + + + + FS A+ PSL A Sbjct: 305 VKGVTTPPPLEVISIDHLPTLLPRESSEAFSEALIPSLLA 344 >SPCP31B10.05 |||tyrosyl-DNA phosphodiesterase |Schizosaccharomyces pombe|chr 3|||Manual Length = 536 Score = 26.2 bits (55), Expect = 4.5 Identities = 30/139 (21%), Positives = 54/139 (38%), Gaps = 4/139 (2%) Frame = -3 Query: 635 PLRKELWLKRICVVLDSTSMM*HSILMPSIEV-VAKSFQQLEDACTHVC*LLS--PVLWS 465 P K+ W CV L+ H+ + P+++V + + D+ + ++ PV Sbjct: 50 PQLKQTWQFNFCVDLNFLLENMHASVFPTVDVRITHGYDSKSDSLARLTAQMNHCPVNVK 109 Query: 464 LYIFVKFSVLK*LWXXXXXXXXXXXXTFSKSPRWQVHLCSL*RPTYLSMSRSVL-LPICV 288 LY SV +W S + +H +L P ++ MS+++ P+ Sbjct: 110 LY-----SVYVPMWGTHHSKIMVNFFK-DDSCQIVIHTANLVEPDWIGMSQAIFKTPLLY 163 Query: 287 PTPADRPSRSAYSTIGRSS 231 P D S S+ G S Sbjct: 164 PKANDSLSTSSVPEYGNPS 182 >SPAC227.16c |||GINS complex subunit Psf3|Schizosaccharomyces pombe|chr 1|||Manual Length = 166 Score = 26.2 bits (55), Expect = 4.5 Identities = 13/47 (27%), Positives = 23/47 (48%) Frame = +2 Query: 542 PLWMASVWSVTS*MLNLTPRKFSSAITPSLAAHWNPATTESLISLRY 682 P W+A V ++ S + P FSS + +L A+ N + + + Y Sbjct: 43 PFWLAEVLAINSFVSIHMPAPFSSVVRNALKANPNSVSIRDITTHYY 89 >SPAC1006.08 |etd1||ethanol-hypersensitive mutant protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 391 Score = 25.8 bits (54), Expect = 6.0 Identities = 11/29 (37%), Positives = 17/29 (58%) Frame = +1 Query: 520 CWNDLATTSMDGISMECYIIDVESNTTQI 606 CW++L+TTS + + II + TT I Sbjct: 211 CWDELSTTSPESSKVSEPIIQDNTQTTHI 239 >SPCC550.05 |nse1||Smc5-6 complex non-SMC subunit 1|Schizosaccharomyces pombe|chr 3|||Manual Length = 232 Score = 25.4 bits (53), Expect = 7.9 Identities = 12/27 (44%), Positives = 14/27 (51%) Frame = -2 Query: 246 HWQVLPGDPCEPQSKPYNVVQETRKRK 166 H+Q L GDP + PY VQ RK Sbjct: 78 HFQNLSGDPVSQMATPYPPVQIELMRK 104 >SPBC1734.15 |rsc4|brd1|RSC complex subunit Rsc4|Schizosaccharomyces pombe|chr 2|||Manual Length = 542 Score = 25.4 bits (53), Expect = 7.9 Identities = 17/67 (25%), Positives = 29/67 (43%), Gaps = 1/67 (1%) Frame = -2 Query: 528 IPTTRRCLYACLLTAQPRLMEPVYL-CEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTP 352 IP T RC+ + + + P+L P L + CP + + V+ H F + P Sbjct: 386 IPNTERCILSAFICSPPQLPLPNPLRMYLPCPSLNSTEV-SVITLAPQHSFLNIVINLNP 444 Query: 351 MFIVKAY 331 +K+Y Sbjct: 445 ALALKSY 451 >SPAC23H4.16c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 328 Score = 25.4 bits (53), Expect = 7.9 Identities = 23/83 (27%), Positives = 39/83 (46%), Gaps = 7/83 (8%) Frame = +1 Query: 442 LNFTKIYRLHKTGLSS*QTCVQASSSCWND-----LAT--TSMDGISMECYIIDVESNTT 600 L+++K++ LH +Q S S +ND LA ++ + + Y++ E + Sbjct: 192 LSYSKVFFLH---------LLQHSDSSFNDKFLLLLANIPVTVSNVEVLLYLLQQEESLA 242 Query: 601 QILFSHNSFLSGPLESSHNRVLN 669 Q + SFL L S HN+V N Sbjct: 243 QFDLNGKSFLYHMLVSLHNQVTN 265 >SPAC11D3.15 |||oxoprolinase |Schizosaccharomyces pombe|chr 1|||Manual Length = 1317 Score = 25.4 bits (53), Expect = 7.9 Identities = 11/31 (35%), Positives = 18/31 (58%) Frame = -2 Query: 681 YLNEIKDSVVAGFQWAAKEGVMAEENLRGVR 589 YL+ + +AGFQ G+ ++N +GVR Sbjct: 248 YLSPVVRRYLAGFQSGFLHGLKTKDNSKGVR 278 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,034,714 Number of Sequences: 5004 Number of extensions: 65578 Number of successful extensions: 156 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 148 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 155 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 323158234 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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