BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fmgV11l18r
(711 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3A12.12 |atp11||F1-ATPase chaperone Atp11 |Schizosaccharomyc... 68 1e-12
SPCC18B5.08c |||isoleucine-tRNA ligase|Schizosaccharomyces pombe... 28 1.1
SPBC20F10.04c |nse4|rad62|Smc5-6 complex non-SMC subunit Nse4|Sc... 27 3.5
SPCC1223.01 ||SPCC285.18|ubiquitin-protein ligase E3 |Schizosacc... 26 4.6
SPBC12C2.02c |ste20|ste16|sterility protein Ste20|Schizosaccharo... 25 8.1
>SPAC3A12.12 |atp11||F1-ATPase chaperone Atp11 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 286
Score = 68.1 bits (159), Expect = 1e-12
Identities = 49/186 (26%), Positives = 86/186 (46%), Gaps = 13/186 (6%)
Frame = -3
Query: 535 KQALGKEINVPDKK-LEDIFKIELVIDKSSTEIKSIWEEYHQNKEVISATIPVDVYSNIK 359
K +G+ D L +E + + ++ I+ +W + +++SA IP ++Y +
Sbjct: 91 KSLVGQNAKKSDLSGLNRYIDVEKIKELPTSTIEKLWRARNIGDDILSACIPKEIYEKML 150
Query: 358 ETMNHFPVFLFPLPR-SQGYEFIMCQM---YGHTVHF--TPLLAYQVHKENAPECLTMVH 197
+P F+ PLPR +G E Q + H T LL Y++ A M+H
Sbjct: 151 SRARMYPYFVLPLPRGDKGIESHFLQWNFPNKNEAHLLVTSLLEYKLKGSYAAPHTIMLH 210
Query: 196 YTELSD-KGIILMRGEYDKNVLNGKEAQCIANQFQMYYSGKD-----PERLKLLESFTKS 35
+ +L + KGI LMR +++ L+ + Q + Q +Y+ + ERL LL +F+K
Sbjct: 211 FADLLNLKGITLMRCQFEPKKLSANDVQLLVLAIQKFYNASENTPLGKERLALLAAFSKG 270
Query: 34 PDTFKH 17
D H
Sbjct: 271 ADFDLH 276
>SPCC18B5.08c |||isoleucine-tRNA ligase|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 973
Score = 28.3 bits (60), Expect = 1.1
Identities = 15/33 (45%), Positives = 21/33 (63%), Gaps = 1/33 (3%)
Frame = -3
Query: 496 KLEDIFKIELV-IDKSSTEIKSIWEEYHQNKEV 401
+L I K+ LV +DK TEI+ ++E Y NK V
Sbjct: 711 ELRGIDKLALVQLDKFQTEIRELYESYSINKVV 743
>SPBC20F10.04c |nse4|rad62|Smc5-6 complex non-SMC subunit
Nse4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 300
Score = 26.6 bits (56), Expect = 3.5
Identities = 12/41 (29%), Positives = 24/41 (58%)
Frame = -3
Query: 487 DIFKIELVIDKSSTEIKSIWEEYHQNKEVISATIPVDVYSN 365
+I I+LV+D + ++I +EY+ + +I PV+ +N
Sbjct: 254 EIRNIQLVLDMTMDLYENIIKEYNIKESIIPTRAPVETSTN 294
>SPCC1223.01 ||SPCC285.18|ubiquitin-protein ligase E3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 732
Score = 26.2 bits (55), Expect = 4.6
Identities = 16/74 (21%), Positives = 37/74 (50%)
Frame = -1
Query: 615 VTRLRKMKQKRNLHLLILGNSHQSLIQNKHSEKKLMCQTRNWRTYLK*NLLLTRVQQKLK 436
V R+ K+ K + L + N +L++NK ++L+ + W+ +L + ++
Sbjct: 538 VARITKLVAKPHEQLSGVFNQIANLLENKEKSRELLEAWQEWK------ILNAKDDTRIG 591
Query: 435 VYGKSIIKIKRSYR 394
+++++KRS R
Sbjct: 592 TTNSNLLRLKRSNR 605
>SPBC12C2.02c |ste20|ste16|sterility protein
Ste20|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1309
Score = 25.4 bits (53), Expect = 8.1
Identities = 7/19 (36%), Positives = 14/19 (73%)
Frame = -3
Query: 694 YYEKYANRIAELQNTSPEE 638
+Y+ ++NR + +QNT P +
Sbjct: 754 FYKPFSNRFSSIQNTKPNQ 772
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,085,830
Number of Sequences: 5004
Number of extensions: 67607
Number of successful extensions: 243
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 226
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 242
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 331187010
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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