BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fmgV11l14f (569 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g34412.1 68417.m04888 expressed protein 31 0.54 At3g18180.1 68416.m02313 hypothetical protein contains Pfam doma... 29 2.2 At3g57190.1 68416.m06367 peptide chain release factor, putative ... 28 5.0 At3g49600.1 68416.m05421 ubiquitin-specific protease 26 (UBP26) ... 27 8.8 >At4g34412.1 68417.m04888 expressed protein Length = 143 Score = 31.1 bits (67), Expect = 0.54 Identities = 25/64 (39%), Positives = 35/64 (54%), Gaps = 3/64 (4%) Frame = -2 Query: 289 DGSCKL-TTFI--TLIG*ADPELVALSKSLLKTETGQLSTRTLSSQKLAQNSVYTEHQTH 119 DGS KL +F+ +LI P L A K+L+ LSTRTL S+ L N ++H T Sbjct: 32 DGSLKLEVSFLNASLIPDIFPLLAAAQKALISKSRDSLSTRTLHSE-LVYNYSGSKHITE 90 Query: 118 A*RR 107 + +R Sbjct: 91 SLKR 94 >At3g18180.1 68416.m02313 hypothetical protein contains Pfam domain, PF04577: Protein of unknown function (DUF563) Length = 470 Score = 29.1 bits (62), Expect = 2.2 Identities = 14/29 (48%), Positives = 17/29 (58%) Frame = -2 Query: 103 SLQTCRVTARRKPAAYLRERTRKRAFRTA 17 SL+ VT RRKP + R+R RAF A Sbjct: 288 SLRNSAVTTRRKPRILILSRSRSRAFVNA 316 >At3g57190.1 68416.m06367 peptide chain release factor, putative similar to SP|P07012 Peptide chain release factor 2 (RF-2) {Escherichia coli}; contains Pfam profile PF03462: PCRF domain Length = 406 Score = 27.9 bits (59), Expect = 5.0 Identities = 10/27 (37%), Positives = 19/27 (70%), Gaps = 1/27 (3%) Frame = +3 Query: 405 LPAGLP-PEPRLWTREDVSVFLKWCER 482 + +G P + ++WT + VS+++KW ER Sbjct: 175 IKSGSPGAKSQIWTEQVVSMYIKWAER 201 >At3g49600.1 68416.m05421 ubiquitin-specific protease 26 (UBP26) similar to GI:11993492; RNA binding protein - Homo sapiens, EMBL:AB016089 (N-terminus), several ubiquitin carboxyl-terminal hydrolases from aa pos. 712 Length = 1067 Score = 27.1 bits (57), Expect = 8.8 Identities = 14/43 (32%), Positives = 21/43 (48%) Frame = +1 Query: 127 GALCTLNSVLTFVMIMYVY*VVQFLFSTRILTTQPVPDQLSRL 255 GA C NS+L + + + F +L PV DQ++RL Sbjct: 112 GATCYANSILQCLYMNTAFREGVFSVEVHVLKQNPVLDQIARL 154 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 11,633,886 Number of Sequences: 28952 Number of extensions: 223797 Number of successful extensions: 601 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 583 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 601 length of database: 12,070,560 effective HSP length: 77 effective length of database: 9,841,256 effective search space used: 1102220672 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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