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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fmgV11l14f
         (569 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At4g34412.1 68417.m04888 expressed protein                             31   0.54 
At3g18180.1 68416.m02313 hypothetical protein contains Pfam doma...    29   2.2  
At3g57190.1 68416.m06367 peptide chain release factor, putative ...    28   5.0  
At3g49600.1 68416.m05421 ubiquitin-specific protease 26 (UBP26) ...    27   8.8  

>At4g34412.1 68417.m04888 expressed protein
          Length = 143

 Score = 31.1 bits (67), Expect = 0.54
 Identities = 25/64 (39%), Positives = 35/64 (54%), Gaps = 3/64 (4%)
 Frame = -2

Query: 289 DGSCKL-TTFI--TLIG*ADPELVALSKSLLKTETGQLSTRTLSSQKLAQNSVYTEHQTH 119
           DGS KL  +F+  +LI    P L A  K+L+      LSTRTL S+ L  N   ++H T 
Sbjct: 32  DGSLKLEVSFLNASLIPDIFPLLAAAQKALISKSRDSLSTRTLHSE-LVYNYSGSKHITE 90

Query: 118 A*RR 107
           + +R
Sbjct: 91  SLKR 94


>At3g18180.1 68416.m02313 hypothetical protein contains Pfam domain,
           PF04577: Protein of unknown function (DUF563)
          Length = 470

 Score = 29.1 bits (62), Expect = 2.2
 Identities = 14/29 (48%), Positives = 17/29 (58%)
 Frame = -2

Query: 103 SLQTCRVTARRKPAAYLRERTRKRAFRTA 17
           SL+   VT RRKP   +  R+R RAF  A
Sbjct: 288 SLRNSAVTTRRKPRILILSRSRSRAFVNA 316


>At3g57190.1 68416.m06367 peptide chain release factor, putative
           similar to SP|P07012 Peptide chain release factor 2
           (RF-2) {Escherichia coli}; contains Pfam profile
           PF03462: PCRF domain
          Length = 406

 Score = 27.9 bits (59), Expect = 5.0
 Identities = 10/27 (37%), Positives = 19/27 (70%), Gaps = 1/27 (3%)
 Frame = +3

Query: 405 LPAGLP-PEPRLWTREDVSVFLKWCER 482
           + +G P  + ++WT + VS+++KW ER
Sbjct: 175 IKSGSPGAKSQIWTEQVVSMYIKWAER 201


>At3g49600.1 68416.m05421 ubiquitin-specific protease 26 (UBP26)
           similar to GI:11993492; RNA binding protein - Homo
           sapiens, EMBL:AB016089 (N-terminus), several ubiquitin
           carboxyl-terminal hydrolases from aa pos. 712
          Length = 1067

 Score = 27.1 bits (57), Expect = 8.8
 Identities = 14/43 (32%), Positives = 21/43 (48%)
 Frame = +1

Query: 127 GALCTLNSVLTFVMIMYVY*VVQFLFSTRILTTQPVPDQLSRL 255
           GA C  NS+L  + +   +    F     +L   PV DQ++RL
Sbjct: 112 GATCYANSILQCLYMNTAFREGVFSVEVHVLKQNPVLDQIARL 154


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,633,886
Number of Sequences: 28952
Number of extensions: 223797
Number of successful extensions: 601
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 583
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 601
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1102220672
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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