BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fmgV11l12r (749 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g03010.1 68418.m00247 kelch repeat-containing protein contain... 30 1.4 At1g76920.1 68414.m08954 F-box family protein (FBX3) contains si... 30 1.4 At3g23630.1 68416.m02972 adenylate isopentenyltransferase 7 / cy... 30 1.9 At2g43880.1 68415.m05455 polygalacturonase, putative / pectinase... 29 4.4 At3g47890.1 68416.m05222 ubiquitin carboxyl-terminal hydrolase-r... 28 5.8 At1g69830.1 68414.m08034 alpha-amylase, putative / 1,4-alpha-D-g... 28 5.8 At3g19440.1 68416.m02465 pseudouridine synthase family protein l... 28 7.6 At1g34130.1 68414.m04234 oligosaccharyl transferase STT3 subunit... 28 7.6 >At5g03010.1 68418.m00247 kelch repeat-containing protein contains Pfam PF01344: Kelch motif (1 repeat) Length = 232 Score = 30.3 bits (65), Expect = 1.4 Identities = 11/40 (27%), Positives = 19/40 (47%) Frame = -2 Query: 373 HNMVVFRNTAGNGALTNWWDNGSNQIAFCRGNQAFIAFNN 254 + +V N AG G +T WW +G + G + + + N Sbjct: 146 YQLVSVANAAGGGRVTVWWKSGLKVLDLLSGTETWECYTN 185 >At1g76920.1 68414.m08954 F-box family protein (FBX3) contains similarity to stamina pistilloidia GI:4101570, the pea ortholog of Fim and UFO from [Pisum sativum] Length = 374 Score = 30.3 bits (65), Expect = 1.4 Identities = 19/64 (29%), Positives = 28/64 (43%), Gaps = 3/64 (4%) Frame = -2 Query: 517 PQLMSSFAFTDTEAGPPMNSRGDITSPTINADNSCGNGWIC--EHRWRQIHNMVVF-RNT 347 P LMSS F + G P S+ + S + W+C +H W I +++ R Sbjct: 201 PVLMSSSVFALCDVGSPWRSQWKLFSCKLTNLTITHTNWVCLEKHEWGDIFDIIKRPRLL 260 Query: 346 AGNG 335 GNG Sbjct: 261 RGNG 264 >At3g23630.1 68416.m02972 adenylate isopentenyltransferase 7 / cytokinin synthase (IPT7) identical to adenylate isopentenyltransferase (IPT7) [Arabidopsis thaliana] GI:14279066 Length = 329 Score = 29.9 bits (64), Expect = 1.9 Identities = 16/53 (30%), Positives = 32/53 (60%), Gaps = 1/53 (1%) Frame = +2 Query: 86 HHVILVAAVDDTSATIAAYYNRFSSAAVTRPGAGNNIAVCSGGETS-LKSLVH 241 HH++ V + + T A Y+R +S A+++ A N + + +GG S +++LV+ Sbjct: 91 HHLLGVFDSEAGNLT-ATQYSRLASQAISKLSANNKLPIVAGGSNSYIEALVN 142 >At2g43880.1 68415.m05455 polygalacturonase, putative / pectinase, putative similar to polygalacturonase 4 [Lycopersicon esculentum] GI:2459815; contains Pfam profile PF00295: Glycosyl hydrolases family 28 (polygalacturonases) Length = 394 Score = 28.7 bits (61), Expect = 4.4 Identities = 25/115 (21%), Positives = 49/115 (42%), Gaps = 2/115 (1%) Frame = -2 Query: 385 WRQIHNMVVFRNTAGNGALTNWWDNGSN--QIAFCRGNQAFIAFNNDAWDMDQTLQTCLP 212 W N++ F+N A N+WD G++ I F + N+ + + +C Sbjct: 81 WGPCKNIITFKNDGTLVAPANYWDIGNSGYWILFAKVNRISV-YGGTIDARGAGYWSCRK 139 Query: 211 AGTYCDIISGARSGNRCTGKSIVVGSDGRARIIHRSNEYDMMVAIHRGADSRL*N 47 G++C GARS + ++++ + N +M V +H ++ R+ N Sbjct: 140 KGSHCP--QGARSISFSWCNNVLLSG------LSSFNSQNMHVTVHHSSNVRIEN 186 >At3g47890.1 68416.m05222 ubiquitin carboxyl-terminal hydrolase-related contains Pfam profiles PF00443: Ubiquitin carboxyl-terminal hydrolase, PF04780: Protein of unknown function (DUF629), PF04781: Protein of unknown function (DUF627) Length = 1568 Score = 28.3 bits (60), Expect = 5.8 Identities = 13/41 (31%), Positives = 28/41 (68%), Gaps = 1/41 (2%) Frame = -2 Query: 124 ARIIHRSNEYDMMVAI-HRGADSRL*NGNRTGKYLKTGNLK 5 AR++ + +E + + A+ ++G+D+ L +G R+G+ K GN + Sbjct: 235 ARLLQQKSECENVGAVDNKGSDATLGSGKRSGERRKHGNAR 275 >At1g69830.1 68414.m08034 alpha-amylase, putative / 1,4-alpha-D-glucan glucanohydrolase, putative similar to SP|P17859 Alpha-amylase precursor (EC 3.2.1.1) (1,4-alpha-D-glucan glucanohydrolase) {Vigna mungo}, alpha-amylase [Malus x domestica] GI:7532799; contains Pfam profile PF00128: Alpha amylase, catalytic domain Length = 887 Score = 28.3 bits (60), Expect = 5.8 Identities = 17/54 (31%), Positives = 29/54 (53%) Frame = -2 Query: 646 ALTFIDNHDNQRGHGAGGNILTYKQSRQYKGAIAFMLAHPYGYPQLMSSFAFTD 485 A+TFI+NHD G+ + + ++ +G A++L HP G P + F+D Sbjct: 766 AVTFIENHDT----GSTQGHWRFPEGKEMQG-YAYILTHP-GTPAVFFDHIFSD 813 >At3g19440.1 68416.m02465 pseudouridine synthase family protein low similarity to SP|P23851 Ribosomal large subunit pseudouridine synthase C (EC 4.2.1.70) (Pseudouridylate synthase) (Uracil hydrolyase) {Escherichia coli}; contains Pfam profile PF00849: RNA pseudouridylate synthase Length = 477 Score = 27.9 bits (59), Expect = 7.6 Identities = 12/29 (41%), Positives = 18/29 (62%) Frame = +3 Query: 105 LRWMIRARPSLPTTIDFPVQRLPDLAPEI 191 LRW++R RP LP T+ + RL + E+ Sbjct: 93 LRWILRCRPDLPRTLVQKLFRLRQVRREM 121 >At1g34130.1 68414.m04234 oligosaccharyl transferase STT3 subunit, putative similar to SP|P39007 Oligosaccharyl transferase STT3 subunit {Saccharomyces cerevisiae}; contains Pfam profile PF02516: Oligosaccharyl transferase STT3 subunit Length = 735 Score = 27.9 bits (59), Expect = 7.6 Identities = 14/38 (36%), Positives = 20/38 (52%) Frame = -2 Query: 355 RNTAGNGALTNWWDNGSNQIAFCRGNQAFIAFNNDAWD 242 +NTA + + +WWD G A GN+ I NN W+ Sbjct: 551 QNTATDAKIMSWWDYGYQITAM--GNRTVIVDNN-TWN 585 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 17,868,104 Number of Sequences: 28952 Number of extensions: 404514 Number of successful extensions: 973 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 941 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 973 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1663169840 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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