BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fmgV11l08f
(605 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 345 3e-96
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 201 7e-53
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 184 9e-48
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 140 1e-34
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 100 3e-22
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 95 5e-21
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 86 3e-18
SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 53 3e-08
SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr... 31 0.13
SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 30 0.23
SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces p... 26 3.7
SPCC1840.12 ||SPCC965.02|OPT oligopeptide transporter family|Sch... 25 8.6
SPBC1683.09c |frp1||ferric-chelate reductase Frp1|Schizosaccharo... 25 8.6
>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
2|||Manual
Length = 375
Score = 345 bits (848), Expect = 3e-96
Identities = 156/167 (93%), Positives = 163/167 (97%)
Frame = +2
Query: 104 NGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPI 283
NGSGMCKAGFAGDDAPRAVFPSIVGRPRH G+MVGMGQKDSYVGDEAQSKRGILTLKYPI
Sbjct: 12 NGSGMCKAGFAGDDAPRAVFPSIVGRPRHHGIMVGMGQKDSYVGDEAQSKRGILTLKYPI 71
Query: 284 EHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNSPA 463
EHGI+ NWDDMEKIWHHTFYNELRVAPEEHP LLTEAPLNPK+NREKMTQI+FETFN+PA
Sbjct: 72 EHGIVNNWDDMEKIWHHTFYNELRVAPEEHPCLLTEAPLNPKSNREKMTQIIFETFNAPA 131
Query: 464 MYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRL 604
YVAIQAVLSLYASGRTTGIVLDSGDGV+HTVPIYEGYALPHAI+RL
Sbjct: 132 FYVAIQAVLSLYASGRTTGIVLDSGDGVTHTVPIYEGYALPHAIMRL 178
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 201 bits (490), Expect = 7e-53
Identities = 91/165 (55%), Positives = 123/165 (74%), Gaps = 1/165 (0%)
Frame = +2
Query: 104 NGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPI 283
NGSG KAGFAGDD P+ +FP+ VGR +H+ VM QKD +VG EAQ+ RG+L ++ PI
Sbjct: 16 NGSGFIKAGFAGDDIPKCLFPTCVGRIKHERVMPSSIQKDMFVGSEAQNLRGLLKIQRPI 75
Query: 284 EHGIITNWDDMEKIWHHTFYNE-LRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNSP 460
E GII NW DME+IW + + ++ L PEEHP+LLTE PL N+EK+ + +ET N P
Sbjct: 76 ERGIIQNWSDMEEIWSYIYSDQQLNTLPEEHPLLLTEPPLANIRNKEKIAEYFYETLNVP 135
Query: 461 AMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAI 595
A+ ++Q VL+LYAS RTTGIVL+ GDG++H+VPIY+G+++P AI
Sbjct: 136 ALSFSLQPVLALYASARTTGIVLECGDGLTHSVPIYDGFSIPSAI 180
>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 390
Score = 184 bits (448), Expect = 9e-48
Identities = 86/169 (50%), Positives = 120/169 (71%), Gaps = 2/169 (1%)
Frame = +2
Query: 104 NGSGMCKAGFAGDDAPRAVFPSIVGRP--RHQGVMVGMGQKDSYVGDEAQSKRGILTLKY 277
NG+G K G+A D+ PR FPSIVGRP R + + KD VGDEA++ R +L +KY
Sbjct: 10 NGTGFVKVGYAKDNFPRFQFPSIVGRPILRAEEKTGNVQIKDVMVGDEAEAVRSLLQVKY 69
Query: 278 PIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNS 457
P+E+GII ++++M ++W +TF+ +L++ P +LLTE P+NP ANREKM + MFE +
Sbjct: 70 PMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPPMNPVANREKMCETMFERYGF 129
Query: 458 PAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRL 604
+YVAIQAVLSLYA G ++G+V+DSGDGV+H VP+YE L H + RL
Sbjct: 130 GGVYVAIQAVLSLYAQGLSSGVVVDSGDGVTHIVPVYESVVLNHLVGRL 178
>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 427
Score = 140 bits (340), Expect = 1e-34
Identities = 81/196 (41%), Positives = 109/196 (55%), Gaps = 32/196 (16%)
Frame = +2
Query: 104 NGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVG---------MGQK------------ 220
NG+G K G+AG+DAP VFP+++ R G G M K
Sbjct: 12 NGTGYSKLGYAGNDAPSYVFPTVIAT-RSAGASSGPAVSSKPSYMASKGSGHLSSKRATE 70
Query: 221 --DSYVGDEAQSKRGI-LTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTE 391
D ++G++A K +L YPI HG I NWD ME+ W + + LR PE+H LLTE
Sbjct: 71 DLDFFIGNDALKKASAGYSLDYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYFLLTE 130
Query: 392 APLNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGRT--------TGIVLDSGDGV 547
PLNP NRE +IMFE+FN +Y+A+QAVL+L AS + TG V+DSGDGV
Sbjct: 131 PPLNPPENRENTAEIMFESFNCAGLYIAVQAVLALAASWTSSKVTDRSLTGTVVDSGDGV 190
Query: 548 SHTVPIYEGYALPHAI 595
+H +P+ EGY + +I
Sbjct: 191 THIIPVAEGYVIGSSI 206
>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 721
Score = 99.5 bits (237), Expect = 3e-22
Identities = 52/160 (32%), Positives = 93/160 (58%), Gaps = 3/160 (1%)
Frame = +2
Query: 104 NGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTL-KYP 280
NGS +AG+ G+ P+ VF ++V R R + + + + VG++ + G ++ + P
Sbjct: 31 NGSWQLRAGWGGEKDPKLVFDNLVSRYRDRK----LSRTSTLVGNDTLIEVGSRSIARSP 86
Query: 281 IEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNSP 460
E +I+NWD ME++ +TF +L + EHP+ +TE NP R MT+++FE +N+P
Sbjct: 87 FERNVISNWDLMEQVLDYTFL-KLGIDRMEHPICMTEPLANPTYVRSTMTELLFELYNAP 145
Query: 461 AMYVAIQAVLSLYASGR--TTGIVLDSGDGVSHTVPIYEG 574
++ I + S Y + + ++GIVL+ G+ SH +P+ G
Sbjct: 146 SVAYGIDGLFSFYHNTKPSSSGIVLNLGNAASHVIPVLNG 185
>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 95.5 bits (227), Expect = 5e-21
Identities = 54/159 (33%), Positives = 82/159 (51%)
Frame = +2
Query: 107 GSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIE 286
GS + GF+G+D P+ V PS G + G + Y+ +S G + +K I
Sbjct: 19 GSKWTRIGFSGEDIPKCVLPSYCGEFSDGRRLFG----EEYI---YKSNPG-MEIKNAIR 70
Query: 287 HGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNSPAM 466
+G + NWD +W + +L+ P EHP+L+TE NP NR K + MFE+ PA
Sbjct: 71 NGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKTLETMFESLRCPAT 130
Query: 467 YVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYAL 583
Y+A Q + +ASG+ T ++D G S IY+G+ L
Sbjct: 131 YLAKQETCAAFASGKGTACLVDIGAERSSVSAIYDGFVL 169
>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
Arp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 430
Score = 86.2 bits (204), Expect = 3e-18
Identities = 46/167 (27%), Positives = 87/167 (52%), Gaps = 2/167 (1%)
Frame = +2
Query: 107 GSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGI--LTLKYP 280
GS + G+AG+++P + PS + GV + ++ YV DE Q I + +K
Sbjct: 18 GSCWTRFGYAGEESPMTILPS------YYGVRSDVTGRNKYVVDELQIHAPIPGMEVKNG 71
Query: 281 IEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNSP 460
+GII +W+ W +L+V P E+ +++TE NP++ R+++ + FE + P
Sbjct: 72 KSNGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITEPSWNPQSVRQQIMEAAFEQLHVP 131
Query: 461 AMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILR 601
A Y+ QAV +A+ ++T +++D G + P+ +G + I +
Sbjct: 132 AFYLTKQAVCVAFANSKSTALIVDIGSDNASVTPVVDGLIIRKGIFK 178
>SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit
Arp9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 523
Score = 53.2 bits (122), Expect = 3e-08
Identities = 31/103 (30%), Positives = 49/103 (47%), Gaps = 4/103 (3%)
Frame = +2
Query: 278 PIEHGIITNWDDMEKIWHHTFYNELRVAPEE----HPVLLTEAPLNPKANREKMTQIMFE 445
PI+ G + +W+ ++ W H Y+ L P + +PV L +RE TQ FE
Sbjct: 113 PIQRGRVVDWEALKAFWKH-LYSLLLKDPNDTTFRYPVCLVIPTYWSLYDRELATQFFFE 171
Query: 446 TFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEG 574
P +A + ++ LYA G G+V+D G + PI +G
Sbjct: 172 ECQVPGFTIAYEPLMGLYAIGILHGLVIDIGYEKTDITPILDG 214
>SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1517
Score = 31.1 bits (67), Expect = 0.13
Identities = 16/48 (33%), Positives = 26/48 (54%)
Frame = +2
Query: 137 GDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYP 280
G+ PRA F ++ P H G+++ M KD G+E S +G + + P
Sbjct: 502 GNQNPRATFVPLLCLPEHGGMVISM--KDWIGGEEFMSPKGFKSPRTP 547
>SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1666
Score = 30.3 bits (65), Expect = 0.23
Identities = 18/53 (33%), Positives = 31/53 (58%), Gaps = 5/53 (9%)
Frame = +2
Query: 338 FYNELRVAPEEHPVLLTE--APLNPKANREKMTQIMFETFNSPAM---YVAIQ 481
+Y L E+HP+LLT+ A L P+ + ++ +I ++ N+P + VAIQ
Sbjct: 1399 YYRALNFYLEQHPMLLTDLLAALTPRIDHPRVIRIFEKSENTPLILNFMVAIQ 1451
>SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 226
Score = 26.2 bits (55), Expect = 3.7
Identities = 10/25 (40%), Positives = 18/25 (72%)
Frame = +2
Query: 380 LLTEAPLNPKANREKMTQIMFETFN 454
+L APL+ + +KM +++FET+N
Sbjct: 181 VLETAPLHAEEVSKKMKELLFETYN 205
>SPCC1840.12 ||SPCC965.02|OPT oligopeptide transporter
family|Schizosaccharomyces pombe|chr 3|||Manual
Length = 791
Score = 25.0 bits (52), Expect = 8.6
Identities = 18/51 (35%), Positives = 25/51 (49%), Gaps = 2/51 (3%)
Frame = -3
Query: 333 WCQIFS-MSSQLVMIPCSMGYFRVRIPLLL-WASSPT*ESFWPIPTITPWW 187
WC+ S + SQ+ + +G F PL L WA + + P ITPWW
Sbjct: 304 WCKPSSAVLSQIFGVKTGLGLF----PLTLDWAQISSLSN----PLITPWW 346
>SPBC1683.09c |frp1||ferric-chelate reductase
Frp1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 564
Score = 25.0 bits (52), Expect = 8.6
Identities = 11/29 (37%), Positives = 17/29 (58%)
Frame = +2
Query: 197 VMVGMGQKDSYVGDEAQSKRGILTLKYPI 283
+M+G +Y+G AQ KR +LT + I
Sbjct: 165 IMIGAIHGFAYIGLAAQGKRALLTARVTI 193
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,274,267
Number of Sequences: 5004
Number of extensions: 43765
Number of successful extensions: 160
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 144
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 152
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 266270664
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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